Cryogen Safety. Dr. Guenter Resch. Online, September 2014
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1 Cryogen Safety Dr. Guenter Resch Online, September 2014
2 Contents Liquid Nitrogen Liquid Ethane and Propane Summary
3 Section 1 Liquid Nitrogen
4 Use of Liquid Nitrogen in EM Direct freezing of cryoprotected specimens Primary coolant for immersion freezing Coolant for instrumentation (HPF, cryomicrotome,... ) Specimen storage Coolant for electron microscopes... Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 4 / 24
5 Physical Properties Melting point 63 K / C Boiling point 77 K / C Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 5 / 24
6 Risks Cold burns Oxygen deficiency Explosion Liquid oxygen condensation Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 6 / 24
7 Cold Burns LN 2 used in laboratories is at its boiling point. Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 7 / 24
8 Cold Burns LN 2 used in laboratories is at its boiling point. LN 2 exhibits Leidenfrost Effect Temporary protection by insulating gas layer Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 7 / 24
9 Prevention of Cold Burns Prevent contact with LN 2 cooled surfaces Prevent (prolonged) contact with LN 2 Prevent LN 2 from being trapped at your body or being soaked up by your clothes. Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 8 / 24
10 Prevention of Cold Burns Leica Microsystems Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 9 / 24
11 Prevention of Cold Burns Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 10 / 24
12 Prevention of Cold Burns Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 10 / 24
13 Oxygen Deficiency 1 l evaporating LN 2 generates 700 l N 2 gas. N 2 displaces O 2 from room atmosphere. Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 11 / 24
14 Oxygen Deficiency 1 l evaporating LN 2 generates 700 l N 2 gas. N 2 displaces O 2 from room atmosphere. 21 % -- Atmospheric O 2 level < 18 % -- Decreased physical and mental performance < 11 % -- Risk of unconciousness, irreversible brain damage, death Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 11 / 24
15 Oxygen Deficiency 1 l evaporating LN 2 generates 700 l N 2 gas. N 2 displaces O 2 from room atmosphere. 21 % -- Atmospheric O 2 level < 18 % -- Decreased physical and mental performance < 11 % -- Risk of unconciousness, irreversible brain damage, death Example: Closed room, m. 10 l LN 2 evaporate at once. Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 11 / 24
16 Oxygen Deficiency 1 l evaporating LN 2 generates 700 l N 2 gas. N 2 displaces O 2 from room atmosphere. 21 % -- Atmospheric O 2 level < 18 % -- Decreased physical and mental performance < 11 % -- Risk of unconciousness, irreversible brain damage, death Example: Closed room, m. 10 l LN 2 evaporate at once. O 2 concentration decreased to 16 %. Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 11 / 24
17 Precautions against Oxygen Deficiency Avoid work with large quantities of LN 2 in confined spaces Only work in well ventilated areas Use oxygen concentration monitors with recent calibration Do not work alone Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 12 / 24
18 Explosion In confined volumes... Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 13 / 24
19 Explosion In confined volumes evaporating LN 2 quickly builds up pressure. Example: A tightly closed container 50 % filled with LN 2 will reach 350 bar -- if it can withstand the pressure for so long. YouTube: "liquid nitrogen bomb" Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 13 / 24
20 Precautions against Explosion Only transport LN 2 in vessels with a loose lid All pressurised LN 2 containers and lines have to be equipped with pressure relief valves Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 14 / 24
21 Liquid Oxygen Condensation O 2 can condense from the air into LN 2 LOx can ignite fires or react with organic materials Do not store LN 2 for long periods in an uncovered container! Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 15 / 24
22 Section 2 Liquid Ethane and Propane
23 Liquid Ethane and Propane Use of Liquid Ethane or Propane in EM Secondary cryogen for Immersion freezing Self-pressurized rapid freezing Used slightly above melting point Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 17 / 24
24 Liquid Ethane and Propane Physical Properties Ethane Melting point 90 K / C Flash point 138 K / C Boiling point 185 K / --89 C Propane Melting point 86 K / C Flash point 169 K / C Boiling point 231 K / --42 C Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 18 / 24
25 Liquid Ethane and Propane Risks Cold burns Explosion Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 19 / 24
26 Liquid Ethane and Propane Cold Burns No Leidenfrost effect! Direct contact with Specimen Skin Eyes Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 20 / 24
27 Liquid Ethane and Propane Explosion Above flash point (--135 C for ethane), ignitable mixtures can form in air. Burn liquefied cryogen under controlled conditions Allow liquefied cryogen to evaporate in a chemical hood Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 21 / 24
28 Section 3 Summary
29 Summary Before Working with Cryogens Understand (!) the risks Familiarize yourself with local safety regulations and follow them Recommended reading: Leica Microsystems: Safety Instructions Liquid Nitrogen (PDF) Cryogen Safety September 2014 (C) Nexperion e.u., 2014 Slide 23 / 24
30 Thank you for your attention. Nexperion e.u. Solutions for Electron Microscopy nexperion
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POLYMER DYNAMIX MATERIAL SAFETY DATA SHEET
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Material Safety Data Sheet
Material Safety Data Sheet Apricot Flavor MSDS 0 He a lt h Fire Re a c t iv it y Pe rs o n a l Pro t e c t io n 0 A Section : Chemical Product and Company Identification Product Name: Apricot Flavor Catalog
2b Product description Black irregular pieces with little or no odor.
Safety Data Sheet Product Names: AGC-30-D, AGC-30 AD, AGC-30 AW, AGC-30 W AGC-40-D, AGC-40 AD, AGC-40 AW, AGC-40 W AGC-50-D, AGC-50 AD, AGC-50 AW, AGC-50 W, AGC-40-MG (Granular Activated Carbon) Effective
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Material Safety Data Sheet
Material Safety Data Sheet NFPA HMIS Personal Protective Equipment 0 Health Hazard Fire Hazard 0 See Section 5. Section. Chemical Product and Company Identification Common Name/ Trade Name Manufacturer
2b Product description Black irregular pieces with little or no odor.
Safety Data Sheet Product Names: AGC-30-D, AGC-30 AD, AGC-30 AW, AGC-30 W AGC-40-D, AGC-40 AD, AGC-40 AW, AGC-40 HP, AGC-40 W AGC-50-D, AGC-50 AD, AGC-50 AW, AGC-50 W, AGC-40-MG (Granular Activated Carbon)
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Date of issue: 04/24/2016 Version: 1.0 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product form Product name : Mixture Product code : 94360
Material Safety Data Sheet
Material Safety Data Sheet Agar-agar MSDS 0 He a lt h Fire Re a c t iv it y Pe rs o n a l Pro t e c t io n 0 A Section : Chemical Product and Company Identification Product Name: Agar-agar Catalog Codes:
Material Safety Data Sheet 1 PRODUCT AND COMPANY IDENTIFICATION 2 COMPOSITION/INFORMATION ON INGREDIENTS
Material Safety Data Sheet 1 PRODUCT AND COMPANY IDENTIFICATION Product Name : Supplier : Kuraray Co., Ltd., Chemicals Company, Elastomer Division, Elastomer Department, Polymer Sales Section Ote Center
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Safety Data Sheet SECTION 1. IDENTIFICATION Product Identifier Other Means of Identification Other Identification Product Family Recommended Use Restrictions on Use Manufacturer / Supplier Emergency Phone
Material Safety Data Sheet
Material Safety Data Sheet Dragon's Blood Powder MSDS 0 He a lt h Fire Re a c t iv it y Pe rs o n a l Pro t e c t io n 0 E Section : Chemical Product and Company Identification Product Name: Dragon's Blood