VARIAN, INC. Flash Chromatography

Transcription

1 VARIAN, INC. Chromatography Isolate compounds from synthesis mixtures quickly and easily Superior purification columns maximize compound purity and recovery Solid loading system enhances gradient accuracy 64

2 Contents 66 Super Purification Columns 67 Super Si Super Si Super Aminopropyl NH2 69 Super C18 69 Super Alumina 70 Puri F Columns 70 DASi Sample Loading Module chromatography purifies reaction products to isolate the target compound. Varian s flash columns are purpose built for purification. Every element has been thought out, custom designed and carefully manufactured by us for excellent purification performance, time after time. 71 Purification Chromatography can be used to purify synthetic compounds for screening as potential drug candidates. 65

3 VARIAN, INC. Super Purification Columns Maximize Compound Purity and Recovery With Unsurpassed Performance and Reliability Sixteen standard sizes plus customized sizes for a wide application range Short, wide columns for speed and long, thin columns for resolution Flat packaging for stock room organization and supply visibility to maintain inventory Each element of the Super compound purification column, with our patented and patent-pending technologies, delivers optimal performance, offering maximum recovery of high purity compounds time after time. Our columns, available in Si 50, Si 35, C18 for normal and reverse phase separations, and a variety of other sorbents, eliminate the common problems of leaking, size limitations, complicated connections and poor compound separation. Instead, you receive a cost-effective, high performance disposable column specifically designed for delivering convenient, efficient separations. Standard Female Luer Lock Easily connects to any system or to a DASi injection cartridge. Identification Stripe Solvent proof (laser etched/no ink) red stripe includes data on column specifications and helpful information for reordering. Normal phase columns have a red stripe, while reverse phase columns have a blue stripe. Solvent Resistant Column Body The column body and cap design use a material that does not swell, warp or crack under prolonged solvent contact. Unitized Construction Patent pending unitized construction hermetically seals the column into a single unit to eliminate leaking up to 200 psi. Dispersion Channels Loads sample evenly onto the column. Standard Male Luer Lock Male luer outlet attaches to any system. 66 For more information, please visit or visit to place an order.

4 Super Purification Columns Media selection Sample Acidic Acid Sensitive Neutral Basic Low or medium Polarity High Polarity Low or medium Polarity High Polarity Low or medium Polarity High Polarity See Also Prep to Process, for flawless purification, page 260 Normal phase (NP) Acidic alumina (NP) Reverse phase (RP) Reverse phase (RP) Neutral alumina (NP) Normal phase (NP) Neutral alumina (NP) Reverse phase (RP) Normal phase (NP) Amine (NP/RP) Basic alumina (NP) Reverse phase (RP) Solvent Polarity Polarity 20 C Solvent Non-polar 0.0 Heptane 0.0 Hexane 0.0 Pentane 0.2 Cyclohexane 1.0 Trichloroethylene 1.6 Carbon tetrachloride 2.8 di-ethyl ether 3.1 Dichloromethane 3.9 Propan-2-ol 4.0 Propan-1-ol 4.0 Tetrahydrofuran 4.1 Chloroform 5.1 Acetone 5.1 Methanol 5.2 Ethanol 5.8 Acetonitrile Polar 9.0 Water Super Si 50, 50 μm particle size, maximum pressure for all columns is 14 bar (200 psi) 1 Model Diameter x Length 2 Flow Rate 3 (ml/min) Sample Load 4 Volume (ml) Quantity (per flat pack/flat packs per case) SF10 4 g 14.2 x mg 6 8/12 AX SF10 8 g 14.2 x mg 11 8/12 AX SF15 12 g 20.8 x mg 1.2 g 16 7/11 AX SF15 24 g 20.8 x mg g 32 7/11 AX SF25 40 g 28.2 x mg 4 g 53 6/9 AX SF25-60 g 28.2 x mg 6 g 79 6/9 AX SF25 80 g 28.2 x mg 8 g 106 6/9 AX SF g 28.2 x g 158 6/9 AX SF g 28.2 x g 211 6/9 AX SF40 80 g 40.6 x mg 8 g 110 4/6 AX SF g 40.6 x g 152 4/6 AX SF g 40.6 x g 200 4/6 AX SF g 40.6 x g 317 4/6 AX SF g 66 x g 264 3/4 AX SF g 66 x g 519 3/4 AX SF g 66 x g 792 3/4 AX For safety obey pressure maximum limits marked on every column. Confirm the instrument has been set to the appropriate maximum pressure before attaching column. 2. Dimensions are for sorbent bed diameter x overall column length. 3. Equilibrium flow rates on 971-FP are typically 40% higher. 4. Sample loading values are suggested. Results may vary with specific samples. For more information, please visit or visit to place an order. 67

5 VARIAN, INC. Super Purification Columns Super Si 35, 35 μm particle size, maximum pressure for all columns is 14 bar (200 psi) 1 Model Diameter x Length 2 Flow Rate 3 (ml/min) Sample Load 4 Volume (ml) Quantity (per flat pack/flat packs per case) SF10 4 g 14.2 x mg 6 8/12 AX SF10 8 g 14.2 x mg 10 8/12 AX SF15 12 g 20.8 x mg 1.2 g 16 7/11 AX SF15 24 g 20.8 x mg g 31 7/11 AX SF25 40 g 28.2 x mg 4 g 52 6/9 AX SF25-60 g 28.2 x mg 6 g 78 6/9 AX SF25 80 g 28.2 x mg 8 g 104 6/9 AX SF g 28.2 x g 156 6/9 AX SF g 28.2 x g 209 6/9 AX SF40 80 g 40.6 x mg 8 g 109 4/6 AX SF g 40.6 x g 150 4/6 AX SF g 40.6 x g 198 4/6 AX SF g 40.6 x g 313 4/6 AX SF g 66 x g 261 3/4 AX SF g 66 x g 512 3/4 AX SF g 66 x g 782 3/4 AX Super Aminopropyl NH2, 40 µm particle size, maximum pressure for all columns is 14 bar (200 psi) 1 Model Diameter x Length 2 SF10-5 g 14.2 x 9.6 AX SF10-10 g 14.2 x 13.6 AX SF15-15 g 20.8 x 11.3 AX SF15-30 g 20.8 x 17.4 AX SF25-50 g 28.2 x 16.3 AX SF25-75 g 28.2 x 22 AX SF g 28.2 x 27.7 AX SF g 28.2 x 39.1 AX SF g 28.2 x 50.6 AX SF g 40.6 x 15.9 AX SF g 40.6 x 20.7 AX SF g 40.6 x 25.5 AX SF g 40.6 x 37.9 AX SF g 66 x 15.7 AX SF g 66 x 26.2 AX SF g 66 x 36.5 AX For more information, please visit or visit to place an order.

6 Super Purification Columns Super C18,18% carbon, end capped on 50 μm particles, maximum pressure for all columns is 14 bar (200 psi) 1 Model Diameter x Length 2 Flow Rate 3 (ml/min) Sample Load 4 Volume (ml) Quantity (column per pack) SF g 14.2 x mg 3 1 AX SF10 10 g 14.2 x mg 5 1 AX SF15 16 g 20.8 x mg 8 1 AX SF15 30 g 20.8 x mg 15 1 AX SF25 55 g 28.2 x mg 27 1 AX SF25 75 g 28.2 x mg 37 1 AX SF g 28.2 x mg 1 g 50 1 AX SF g 28.2 x mg 1.5 g 74 1 AX SF g 28.2 x mg 2 g 99 1 AX SF g 40.6 x mg 1 g 50 1 AX SF g 40.6 x mg 1.5 g 74 1 AX SF g 40.6 x mg 2 g AX SF g 40.6 x mg - 3 g AX SF g 66 x mg 3 g AX SF g 66 x g AX SF g 66 x g AX Super Alumina, 125 µm particle size, maximum pressure for all columns is 14 bar (200 psi) 1 Model Diameter x Length 2 Sample Load 4 Alumina Neutral Alumina Acidic Alumina Basic SF10-8 g 14.2 x mg AX (8/Flat pack) AX (8/Flat pack) AX (8/Flat pack) SF10-16 g 14.2 x mg AX (8/Flat pack) AX (8/Flat pack) AX (8/Flat pack) SF15-24 g 20.8 x mg 1.2 g AX (7/Flat pack) AX (7/Flat pack) AX (7/Flat pack) SF15-48 g 20.8 x mg 2.2 g AX (7/Flat pack) AX (7/Flat pack) AX (7/Flat pack) SF25-80 g 28.2 x mg 2.2 g AX (6/Flat pack) AX (6/Flat pack) AX (6/Flat pack) SF g 28.2 x g AX (6/Flat pack) AX (6/Flat pack) AX (6/Flat pack) SF g 28.2 x g AX (6/Flat pack) AX (6/Flat pack) AX (6/Flat pack) SF g 28.2 x g AX (6/Flat pack) AX (6/Flat pack) AX (6/Flat pack) SF g 28.2 x g AX (6/Flat pack) AX (6/Flat pack) AX (6/Flat pack) SF g 40.6 x g AX (4/Flat pack) AX (4/Flat pack) AX (4/Flat pack) SF g 40.6 x g AX (4/Flat pack) AX (4/Flat pack) AX (4/Flat pack) SF g 40.6 x g AX (4/Flat pack) AX (4/Flat pack) AX (4/Flat pack) SF g 40.6 x g AX (4/Flat pack) AX (4/Flat pack) AX (4/Flat pack) SF g 66 x g AX (3/Flat pack) AX (3/Flat pack) AX (3/Flat pack) SF g 66 x g AX (3/Flat pack) AX (3/Flat pack) AX (3/Flat pack) SF g 66 x g AX (3/Flat pack) AX (3/Flat pack) AX (3/Flat pack) 1. For safety obey pressure maximum limits marked on every column. Confirm the instrument has been set to the appropriate maximum pressure before attaching column. 2. Dimensions are for sorbent bed diameter x overall column length. 3. Equilibrium flow rates on 971-FP are typically 40% higher. 4. Sample loading values are suggested. Results may vary with specific samples. For more information, please visit or visit to place an order. 69

7 VARIAN, INC. Puri F DASi Module Puri F Columns: Cartridges for Compression Module Systems Available in a variety of sizes for development systems Convenient sorbents to meet your needs Consistent packing for less channelling and fraction dilution If you regularly purify more than a few grams of compound, Puri F development flash cartridges deliver the convenience and compatibility you need. The cartridges are packed with silica for normal phase separations and silica C18 for reverse phase purifications. For development scale they are available in different bed diameters and bed masses to provide solutions for a range of sample sizes. Puri F75 cartridges Model Sorbent Quantity F75S 200 g Si 50 2 AX F75S 200 g Si AX F75S 200 g Si 35 2 AX F75S 200 g Si AX F75S 300 g C18 1 AX F75M 400 g Si 50 2 AX F75M 400 g Si AX F75M 400 g Si 35 2 AX F75M 400 g Si AX F75M 600 g C18 1 AX F75L 800 g Si 50 2 AX F75L 800 g Si AX F75L 800 g Si 35 2 AX F75L 800 g Si AX F75L 1.2 kg C18 1 AX F75XL 1.6 kg Si 50 2 AX Puri F150 cartridges DASi Sample Loading Module: For Even Loading of Low Solubility and High Viscosity Compounds Modules are available in three sizes to match your sample needs Adjustable plunger eliminates dead volume and maintains gradient accuracy Provides security as a guard column for high cost specialty sorbent columns DASi module kits Standard Female Luer Lock Easily connect the DASi to any system with a female Luer lock top fitting. Patent Pending Locking Mechanism Easily push piston down. Assembly will remain in position until released. Adjustable Plunger Head Eliminates dead volume to maintain the superior gradient accuracy of the 971-FP (especially important for DCM/methanol solvent combination). Dispersion Channels These distribute solvent evenly on the sample bed for tight, thin separation bands. Standard Male Luer Lock Male Luer outlet attached to any system or to the top of a Super column. Solvent Compatible Cartridge The cartridge body does not swell, warp, or crack under prolonged solvent contact. Model Sorbent Quantity F150M 2.5 kg Si 50 2 AX F150M 2.5 kg Si AX F150M 2.5 kg Si 35 2 AX F150M 2.5 kg Si AX F150M 3.9 kg C18 1 AX F150L 5 kg Si 50 2 AX F150L 5 kg Si AX F150L 5 kg Si 35 2 AX F150M 5 kg Si AX F150L 9 kg C18 1 AX DASI 12 DASi 35 DASi 65 DASi 12, 35 and 65 DASi consumables SI sampler pack DASI 12 DASi 35 DASi 65 AX AX AX AX AX AX AX For more information, please visit or visit to place an order.

8 Purification Excellent Purification Performance of UV Active Compounds at Different Wavelengths Eliminates uncontrolled sample loss to ensure sample security and retention Method-guiding functionality optimizes solvent, column and gradient options for increased efficiency and flexibility Ready to Run technology reduces downtime The 971-FP system enhances productivity through its readyto-run technology that eliminates warm-up time, performs self diagnostics to ensure proper operation, supplies helpful navigation run start-up software and introduces walk away start-up features like system auto-prime and sample auto-inject. The instrument incorporates the latest compound separation innovations, and contains new sample security and retention technology. Instrument Supplies We offer several accessories to support the Varian 971-FP, including a Multi-column Controller to connect additional stations for uninterrupted column operation. The Advanced Feature Package (AFP) offers uninterrupted solvent supply, waste level monitoring and feedback, Guide Me functionality and dynamic run queues for multi-column control capacity. A high-speed processor and advanced operating software are integral to the AFP. The integral fume enclosure traps solvent fumes for use in areas without hoods (requires a 4 in. or greater exhaust ventilation connection), and the solvent bottle safety tray provides additional support for storing 4 L solvent bottles. 971-FP Single Wavelength UV Chromatography Workstation with Intelli Software, Binary Gradient Pump and Fraction Collector 971-FP Multi-wavelength UV Chromatography Workstation with Intelli Software, Binary Gradient Pump and Fraction Collector MCC2 - Multi-column Controller, Includes One Column Station 65 AFP310 Advanced Feature Package IFE310 - Integral Fume Enclosure SBT310 - Solvent Bottle Safety Tray AX AX AX AX AX AX See Also Puri F, for purifying larger quantities, page 70 A variety of accessory racks for the Varian 971-FP, all with radio frequency identification (RFID) technology, is also available. 13 x 100 mm Rack, Holds 90 Tubes AX x 100 mm Rack, Holds 60 Tubes AX x 150 mm Rack, Holds 60 Tubes AX x 150 mm Rack, Holds 40 Tubes AX x 150 mm Rack, Holds 24 Tubes AX For more information, please visit or visit to place an order. 71