Phyto-toxicological Effects of Copper Nanoparticles in Bell Pepper (Capsicum annum) plants

Transcription

1 Phyto-toxicologicl Effects of Copper Nnoprticles in Bell Pepper (Cpsicum nnum) plnts Swti Rwt ESE PhD Student Grde Group, University of Texs t El Pso Sustinble Nnotechnology Orgniztion Orlndo, FL, November,

2 Structure of the presenttion Introduction - Nnoprticles - Bell pepper plnts Methodology Results Conclusions 2

3 Introduction 3

4 Fctors ffecting NPs induced toxicity towrds terrestril plnts Size C 2+ Mg 2+ nzno nceo 2 nsi MWCNT s Surfce chrge H C=O COO H + OH - P H Structure nd defects Reddy, P. V. L., Hernndez-Viezcs, J. A., Perlt-Vide, J. R., & Grde-Torresdey, J. L. (2016). Lessons lerned: Are engineered nnomterils toxic to terrestril plnts?. Science of The Totl Environment, 568,

5 Copper Nnoprticles (NPs) SEM Microgrphs of copper nnoprticles 5 Hong, Jie, Cyren M. Rico, Lijun Zho, Adeyemi S. Adeleye, Arturo A. Keller, Jose R. Perlt-Vide, nd Jorge L. Grde-Torresdey. "Toxic Effects of Copper-Bsed Nnoprticles Or Compounds to Lettuce (Lctuc Stiv) nd Alflf (Medicgo Stiv)." Environmentl Science: Processes & Impcts 17, no. 1 (2015):

6 Applictions of Copper NPs 6

7 Globl flows for Cu nd oxides of Cu (metric tons/yr) in 2010 Keller, Arturo A., Suznne McFerrn, Anstsiy Lzrev, nd Sngwon Suh. "Globl Life Cycle Releses of Engineered Nnomterils." Journl of Nnoprticle Reserch 15, no. 6 (2013):

8 Bell pepper plnts Cpsicum nnum Rich in nti-oxidnts like crotenoid, sugrs, vitmin C. Fruit is 92% wter, rest re crbohydrtes nd smll mount of protein nd ft 8

9 Methodology 9

10 Soil Soil collected on the est side of El Pso, TX. Soil chrcteriztion conducted on Mlvern Mstersizer Hybrid 2000G - Snd : 19.7 % - Silt : % - Cly : % Nturl soil : silt lom 10

11 Sowing seeds t the green house for seedling trnsplnttion 11

12 Prepring pots in the lb 12

13 Plnt growth stges : full growth cycle 90 dys Seedlings growing Plnts 10 dys post trnsplnttion Seedlings redy for trnsplnttion Freshly trnsplnted seedlings 13

14 Plnt growth stges : full growth cycle 90 dys Plnts 30 dys post trnsplnttion Fully mtured plnts, 90 dys post trnsplnttion Plnts 45 dys post trnsplnttion, flowering Plnts 60 dys post trnsplnttion, fruiting 14

15 Conditions t the green house Controlled environment, temperture, reltive humidity, nd light intensity - Averge light 10.1 mol/m 2 /d - Averge dy temperture 27.2±1.6 C - Averge night temperture 25±2.1 C Wter every other dy, or s need be with fertilizer solution, rtio of N-P 2 O 5 -K 2 O, ph: 5.8, EC: 1.00 ms/cm Abmectin, Avid 0.15 EC, to tret phids or white fly 15

16 Hrvesting 16

17 Gs exchnge mesurement: LI-6400XT portble photosynthesis system 17

18 18

19 Acid digestion nd smple nlysis on the ICP-OES 19

20 Results 20

21 Reltive Chlorophyll Content (SPAD) Chlorophyll content, vs ionic copper tretments 80 Chlorophyll Content Control Concentrtion of the tretments, mg/kg 21

22 Evpotrnspirtion mmol/m 2 /s Gs Exchnge : Evpotrnspirtion, vs ionic copper tretments Evpotrnspirtion 6 b b 5 b b b 1 0 Control Concentrtion of the tretments, mg/kg 22

23 Stomtl Conductnce mol/m2/s Gs Exchnge: Stomtl conductnce, vs ionic copper tretments 500 Stomtl Conductnce c bc 350 bc bc 300 bc b 50 0 Control Concentrtion of the tretments, mg/kg 23

24 Photosynthesis µmol/m 2 /s Gs exchnge : Photosynthesis, vs ionic copper tretments Photosynthesis b b b b b b b Control Concentrtion of the tretments, mg/kg 24

25 Elementl nlysis of root smples, copper c mg of Cu/kg of root dry wt b b b b b b b 0 Control Control Control Control Tretment Concentrtion, mg/kg 25

26 mg of Cu/kg leves dry wt. Elementl nlysis of leves smples, copper 30 c bc bc bc b bc bc bc Control Control Control 250 Tretment Concentrtion, mg/kg 250 Control

27 mg of Cu/kg of fruit dry wt. Elementl nlysis of fruit smples, copper Control Control Control Control Tretment Concentrtion, mg/kg 27

28 Conclusions Gs exchnge : evpotrnspirtion, stomtl conductnce, nd photosynthesis were not significntly different with respect to the control but were sttisticlly different with respect to ech other t the different concentrtions of nd CuCl 2. The copper content in root smples ws significntly incresed t 125 mg/kg CuCl 2, 250 mg/kg nd CuCl 2, nd t 500 mg/kg nd CuCl 2 wrt the control. The two tretments were significntly different t the highest concentrtion. The lef smples found significntly higher mount of copper t 250 mg/kg nd 500 mg/kg concentrtion of both the compounds wrt the control. Significntly higher mount of copper ws found in the fruit smples t 125 mg/kg ionic tretment. 28

29 Acknowledgements UCCEIN for funding the reserch Texs AnM Agrilife Reserch nd Extension Centre t El Pso, TX. University of Texs t El Pso Lb Mtes Fculty - Dr Youping Sun - Dr Jose A. Hernndez - Dr Jose R. Perlt - Dr Jorge Grde Torresdy The SNO conference orgnizers 29

30 References Reddy, P. Venkt Lxm, J. A. Hernndez-Viezcs, J. R. Perlt-Vide, nd J. L. Grde-Torresdey. "Lessons Lerned: Are Engineered Nnomterils Toxic to Terrestril Plnts?" Science of the Totl Environment 568, (10/15, 2016): Hong, Jie, Cyren M. Rico, Lijun Zho, Adeyemi S. Adeleye, Arturo A. Keller, Jose R. Perlt-Vide, nd Jorge L. Grde- Torresdey. "Toxic Effects of Copper-Bsed Nnoprticles Or Compounds to Lettuce (Lctuc Stiv) nd Alflf (Medicgo Stiv)." Environmentl Science: Processes & Impcts 17, no. 1 (2015): Keller, Arturo A., Suznne McFerrn, Anstsiy Lzrev, nd Sngwon Suh. "Globl Life Cycle Releses of Engineered Nnomterils." Journl of Nnoprticle Reserch 15, no. 6 (2013):

31 Thnk You! Questions? 31