UV/Vis/NIR Certified Reference Materials

Transcription

1 UV/Vis/NIR Certified Reference Materials SPRING 2015 ISO/IEC Accredited ISO Guide 34 USB Drive supplied with each set contains the reference certificate and all vital information required

2 Why do you need to use reference materials? Ultraviolet, visible and NIR spectrophotometers are fundamentally both accurate and precise but it is vital to check instrument performance on a regular basis to ensure operation is within satisfactory parameters. References provided by Starna are based on materials, which have been used or produced by a National Metrology Institute (NMI), such as the National Institute of Standards and Technology (NIST) or fundamental metrological principles. When used in routine testing of instrument performance, a quality control database of the operating characteristics for the specific instrument can be built. This database can then be used for the following purposes: * Demonstration of satisfactory instrument performance to any inspection or regulatory agency. * Detection of instrument malfunction. * Provide data to a Customer Support engineer allowing detection of instrument faults and assistance in repair. Each spectrophotometer has specific operating characteristics based on the geometry and basic design of the optical system. These reference materials become your guide to the efficient operation of your instrument. What makes Starna reference materials unique? All sealed, Glass Filters or Metal on Quartz reference materials produced by Starna are Ready to Use. References are supplied in either heat fusion sealed quartz cells or, in the case of the Glass or Metal on Quartz Filters, in an aluminum holder with the same external dimensions as a standard spectrophotometer cell. Starna's sealed references are environmentally friendly as they eliminate the need to open an ampoule, use it once and throw it away. Wet chemistry methods are no longer required to make up the references from raw materials. With care, Construction of reference materials the sets will last for many years. All Certified Reference Material (CRM) sets can be recertified. Properties of Starna heat fusion sealed Quartz cells, glass or Metal on Quartz references: 1) Sealed cells ensure cleanliness, repeatability and reliability 2) They can be used multiple times over many years 3) The range covers the Far UV, UV, Visible and NIR 4) All Certified Reference Materials (CRMs) can be recertified. Starna has over forty-five years of experience pioneering the technology of sealing reference materials into quartz spectrophotometer cells. The technology allows the use of materials in the UV range where Glass Filters are not usable. The sealed reference cells have the same optical characteristics as cells used in normal analysis thus removing many variables, which might be introduced using any other form of optical configuration or reference preparation. The references have a lifetime guarantee subject to specified conditions and are supplied in suitable storage containers. The Glass Filters are made to the highest industry standards with raw material of known characteristics. UKAS ISO/IEC Accredited CRMs NIST (National Institute for Standards and Technology in Washington DC, USA) traceability is available on all our reference materials, unless otherwise indicated. Traceability is established during the calibration of each of our five reference spectrophotometers using NIST primary Standard Reference Materials (SRMs). Documentation supplied with Starna references details the analysis procedure, NIST traceability, the analyzed values of the reference and the confidence limits. Certification supplied with Starna reference materials is suitable for use with all inspecting agencies when Should I use Starna CRMs? Traceability to a NMI allows for a definitive, certifiable method of analysis of the reference using the same methods as the NMI. Additionally, each reference raw material is either purchased from NIST (where available) or directly tested against a NIST supplied reference. The most important element of traceability is the Starna certificate of analysis and traceability. used in the context of standard operating procedures for quality control, established in your laboratory. Our calibration laboratory is inspected and accredited by UKAS under ISO/ IEC for the Starna calibration laboratory and under ISO Guide 34 as a Reference Material producer. This level of accreditation assures the user that the certification of our reference materials is of the highest standard and may be used to validate instrument performance in all ISO/IEC and/or ISO/IEC regulated environments. This certificate details the method of analysis and the certified results of the analysis. You can use these certified results as the basis of your quality program. If your lab is routinely monitored by a certifying agency, then the Starna level of accreditation and accredited traceability is invaluable. 2 Starna Scientific

3 Table of Contents: Ultraviolet References Material: Used for: Traceable to: Configuration: Page(s): Potassium Dichromate Absorbance, Linearity, UV SRM 935a Filled Quartz Cell 4, 5, 6 & 7 Nicotinic Acid Absorbance, Far UV SRM 935a Filled Quartz Cell 8 & 9 Metal on Quartz Filters Absorbance, Linearity, UV/VIS SRM 2301 Metal Coated Quartz Window 10 Starna Green Absorbance, Wavelength, Linearity UV/VIS SRM 935a/2034 Filled Quartz Cell 11 Sodium Chloride Stray light Far UV SRM 2032 Filled Quartz Cell 18 & 19 Potassium Chloride Stray light Far UV SRM 2032 Filled Quartz Cell 18 & 19 Potassium Iodide Stray light UV SRM 2032 Filled Quartz Cell 18 & 19 Sodium Iodide Stray light UV SRM 2032 Filled Quartz Cell 18 & 19 Lithium Carbonate Stray light UV SRM 2032 Filled Quartz Cell 18 & 19 Sodium Nitrite Stray light VIS SRM 2032 Filled Quartz Cell 18 & 19 Acetone Stray light VIS SRM 2032 Filled Quartz Cell 18 & 19 Holmium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 12 Didymium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 13 Samarium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 14 Rare Earth Oxide Wavelength Far UV SRM 2034 Filled Quartz Cell 15 Benzene Vapor Resolution not controlled Filled Quartz Cell 20 Toluene in Hexane Resolution SRM 935a Filled Quartz Cell 21 Visible References Material Used for Traceable to: Configuration: Page: Neutral Density Glass Filters Absorbance, Linearity, VIS SRM 930e Glass Filters 22, 23 & 25 Holmium Glass Filter Wavelength VIS SRM 2034 Glass Filter 24 & 25 Didymium Glass Filter Wavelength VIS SRM 2034 Glass Filter 24 Near Infrared Material Used for Traceable to: Configuration: Page: Metal on Quartz Filters Absorbance, Linearity, NIR SRM 2301 Metal Coated Quartz Window 16 NIR Wavelength material Wavelength, NIR SRM 2065 Filled Quartz Cell 17 DNA and RNA 260/280 Ratio References Material Used for Traceable to: Configuration: Page: Stable Aqueous 260/280 Absorbance UV SRM 935a Filled Quartz Cell 28 Stable Aqueous 260/280 Absorbance UV SRM 935a Vials, Screw or Crimp top 29 Microplate Reader References Material Used for Traceable to: Configuration: Page: Glass Filters Wavelength, Absorbance, VIS SRM 2034, 930e Glass Filters 26 Adaptor To facilitate the use of standard sealed quartz cells or glass filters to validate Microplate Readers 27 Fluorescent & Raman References Material Used for Traceable to: Configuration: Page: Quinine Sulphate Solution Wavelength, Intensity & Linearity SRM 2034, 930e Filled Quartz Cell 30 Ultra High Purity Water Fluorescent Instrument Sensitivity Filled Quartz Cell 31 References for use with methods described in... Material Test Range USP Ph. Eur. DAB BP ASTM TGA Holmium Oxide Wavelength UV/Visible Toluene in Hexane Spectra Resolution UV Potassium Chloride Stray Light UV Sodium Iodide Stray Light UV Potassium Iodide Stray Light UV Potassium Dichromate Absorbance UV Neutral Density Filters Absorbance Visible 3

4 Potassium Dichromate - UV - Absorbance/Linearity up to 1.5A Potassium Dichromate from NIST (SRM 935a), permanently heat fusion sealed in quartz cells, CRM complete with UKAS ISO/IEC accredited certificate of calibration. Primary Usage: Testing absorbance and linearity scale in the UV region of the spectrum up to 1.5 Absorbance. Certified Wavelengths: 235, 257, 313 and 350 nm for 20mg/L to 100mg/L. 430 nm for 600mg/L. UV quartz cells that have been permanently heat fusion sealed. Product Potassium Dichromate solvated in dilute perchloric acid is deemed to be the best method for testing the absorbance scale and linearity of spectrophotometers in the UV. Solid Potassium Dichromate used by Starna is purchased directly from NIST (SRM 935a). Solutions are made up in accordance to NIST certification and then permanently heat fusion sealed in Far UV quartz spectrophotometer cells. The filled cells are tested and certified against the expected values from NIST. A certificate is provided with each set of cells, which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for quality control, established in your laboratory. Each set is supplied with a sealed blank cell used to obtain the certified measurements. The following chart is a listing of approximate absorbance values for each concentration based on the wavelength at which the Potassium Dichromate cells are to be read: Concentration 235 nm 257 nm 313 nm 350 nm 430 nm 20 mg/l n/a 40 mg/l n/a 60 mg/l n/a 80 mg/l n/a 100 mg/l n/a European Pharmacopoeia compliance requires the following additional reference: 600mg/L n/a n/a n/a n/a Suggestions for Use: Scan the absorbance of the Potassium Dichromate cells over the certified wavelength range, against the blank supplied. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the four wavelengths. If you determine that your instrument is not giving you the correct values, consult your Customer Support engineer for advice on how to determine and correct any problem, which this reference may have detected. For expanded uncertainty budget and expected parameters see page 35 Description Part Number Linearity Dichromate set: Potassium Dichromate, 5 concentrations & Blank Cell 20, 40, 60, 80 & 100mg/L & Blank Cell RM Single Concentrations: Potassium Dichromate, 20 mg/l & Blank Cell Potassium Dichromate, 40 mg/l & Blank Cell Potassium Dichromate, 60 mg/l & Blank Cell Potassium Dichromate, 80 mg/l & Blank Cell Potassium Dichromate, 100 mg/l & Blank Cell RM-02 RM-04 RM-06 RM-08 RM-10 Additional concentration required for European Pharmacopoeia Compliance: Potassium Dichromate, 600 mg/l RM-60 Linearity Dichromate set, with additional concentration for European Pharmacopoeia Compliance: Potassium Dichromate, 6 concentrations & Blank Cell RM Starna Scientific

5 Potassium Dichromate - UV - Absorbance/Linearity up to 1.5A RM set: This CRM set of one blank and five increasing concentrations of Potassium Dichromate is our most useful and widely purchased set consisting of 6 cells with the following materials: Cell# Containing Concentration 1 Perchloric acid blank 0.001N 2 Potassium Dichromate 20 mg/l 3 Potassium Dichromate 40 mg/l 4 Potassium Dichromate 60 mg/l 5 Potassium Dichromate 80 mg/l 6 Potassium Dichromate 100mg/L Spectral scan: The graph to the left is a scan of 5 different concentrations of Potassium Dichromate. It clearly shows the peaks and troughs in relation to absorbance and the differences due to Potassium Dichromate concentration. Peaks: 257 and 350 nm Troughs: 235 and 313 nm The certificate supplied with the CRM sets lists the absorbance values for each peak and trough at the four wavelengths measured against the perchloric acid blank at the four wavelengths. When measured in your spectrophotometer you can validate your transmittance/absorbance scale for accuracy and linearity using the set with 5 different concentrations and the blank. Linearity: Plotting concentration against Absorbance using the set of 5 cells against the blank, you can test your spectrophotometer for linearity through the UV range. The use of the CRM set with 5 different concentrations and a blank is recommended for best results. 5

6 Potassium Dichromate - UV - Absorbance/Linearity up to 3.3A Potassium Dichromate from NIST (SRM 935a), permanently heat fusion sealed in quartz cells, CRM complete with UKAS ISO/IEC accredited certificate of calibration. Primary Usage: Testing absorbance and linearity scale in the UV region of the spectrum up to 3.3 Absorbance. Certified Wavelengths: 235, 257, 313 and 350 nm for 20mg/L to 240mg/L. UV quartz cells that have been permanently heat fusion sealed. Product Potassium Dichromate solvated in dilute perchloric acid is deemed to be the best method for testing the absorbance scale and linearity of spectrophotometers in the UV. Solid Potassium Dichromate used by Starna is purchased directly from NIST (SRM 935a). Solutions are made up in accordance to NIST certification and then permanently heat fusion sealed in Far UV quartz spectrophotometer cells. The filled cells are tested and certified against the expected values from NIST. A certificate is provided with each set of cells, which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for quality control, established in your laboratory. Each set is supplied with a sealed blank cell that was used to obtain the certified measurements. Suggestions for Use: The following chart is a listing of approximate absorbance values for concentrations 120mg/l or higher, based on the wavelength at which the Potassium Dichromate cells are to be read: Concentration 235nm 257 nm 313 nm 350 nm 120 mg/l mg/l mg/l mg/l mg/l mg/l mg/l Scan the absorbance of the Potassium Dichromate cells over the certified wavelength range against the blank supplied. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the four wavelengths. If you determine that your instrument is not giving you the correct values, consult your Customer Support engineer for advice on how to determine and correct any problem, which this reference may have detected. For expanded uncertainty budget and expected parameters see page 35 Description Part Number Linearity Dichromate set from 1.5 to 3.5A Potassium Dichromate, 6 concentrations & Blank Cell 120, 140, 160, 180 & 220 & 240mg/L & Blank RM Linearity Dichromate set from 0.19 to 3.5A Potassium Dichromate, 6 concentrations & Blank Cell 40, 80, 120, 160, 200 & 240 mg/l & Blank RM Customer specified Potassium Dichromate Concentration options, including those up to 1.5A from page 4: Potassium Dichromate, 1 concentration & Blank Potassium Dichromate, 2 concentrations & Blank Potassium Dichromate, 3 concentrations & Blank Potassium Dichromate, 4 concentrations & Blank Potassium Dichromate, 5 concentrations & Blank Potassium Dichromate, 6 concentrations & Blank Potassium Dichromate, 7 concentrations & Blank 6 Starna Scientific

7 Potassium Dichromate - UV - Absorbance/Linearity up to 3.3A RM set: This CRM set of one blank and six increasing concentrations of Potassium Dichromate is our most useful and widely purchased set consisting of 7 cells with the following materials: Cell# Containing Concentration 1 Perchloric acid blank 0.001N 2 Potassium Dichromate 120 mg/l 3 Potassium Dichromate 140 mg/l 4 Potassium Dichromate 160 mg/l 5 Potassium Dichromate 180 mg/l 6 Potassium Dichromate 220 mg/l 7 Potassium Dichromate 240 mg/l Spectral scan: The graph to the left is a scan of 11 different concentrations of Potassium Dichromate. It clearly shows the peaks and troughs in relation to absorbance and the differences due to Potassium Dichromate concentration. Peaks: 257 and 350 nm Troughs: 235 and 313 nm The certificate supplied with the CRM sets lists the absorbance values for each peak and trough at the four wavelengths measured against the perchloric acid blank at the four wavelengths. When measured in your spectrophotometer you can validate your absorption scale for accuracy and linearity using the set with 5 different concentrations and the blank. Linearity: Plotting concentration against absorption using the set of 5 cells against the blank, you can test your spectrophotometer for linearity through the UV range. The use of the CRM set with 5 different concentrations and a blank is recommended for best results. 7

8 Nicotinic Acid - Far UV - Absorbance/Linearity Primary Usage: Certified Wavelengths: Nicotinic Acid, Far UV Absorbance standard, sealed in quartz cells, CRM complete with UKAS ISO/IEC accredited certificate of calibration Testing absorbance scale and linearity in the Far UV region of the spectrum. 213 nm, 261 nm UV quartz cells that have been permanently heat fusion sealed Product The use of Nicotinic Acid solvated in dilute hydrochloric acid is a well documented method for the validation of the absorbance scale and linearity of a spectrophotometer in the Far UV region. When prepared in 0.1M hydrochloric acid, Nicotinic Acid gives a spectral scan containing characteristic peaks at approx. 210 nm and 260 nm. Within the concentration range 5-25 mg/l, if the absorbance scale of a narrow SBW (2 nm) spectrophotometer is linear, the apparent absorbances of a series of concentrations will be a linear function of concentration, at a specified SBW. The filled cells are tested and certified against the calculated values. A certificate is provided with each set of cells which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for the quality control that is established in your laboratory. Each set is supplied with a sealed blank cell that was used for the certified measurement. The following chart is a listing of approximate absorbance values for each concentration based on the wavelength at which the Nicotinic Acid cells are to be read at 1 nm SBW*: Concentration 213 nm 261 nm 6 mg/l mg/l mg/l mg/l * Alternative SBW values available on request. Suggestions for Use: Scan the absorbance of the Nicotinic Acid cells over the certified wavelength range against the blank supplied. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the two wavelengths. If you determine that your instrument is not giving you the correct values, consult your service technician for advice on how to determine and correct any problem, which this reference may have detected. For expanded uncertainty budget and expected parameters see page 35 Description Part Number Linearity Nicotinic Acid set: Nicotinic Acid, 4 concentrations & Blank Cell Single Concentrations: Nicotinic Acid, 6 mg/l & Blank Cell Nicotinic Acid, 12 mg/l & Blank Cell Nicotinic Acid, 18 mg/l & Blank Cell Nicotinic Acid, 24 mg/l & Blank Cell RM-1A2A3A4A RM-1A RM-2A RM-3A RM-4A 8 Starna Scientific

9 Nicotinic Acid - Far UV - Absorbance/Linearity RM-1A2A3A4A set: This CRM set of one blank and four increasing concentrations of Nicotinic Acid is unique in its ability for the verification of the Far UV absorbance scale. It consists of 5 cells with the following materials: Cell# Containing Concentration 1 Hydrochloric acid blank 0.1M 2 Nicotinic Acid 6 mg/l 3 Nicotinic Acid 12 mg/l 4 Nicotinic Acid 18 mg/l 5 Nicotinic Acid 24 mg/l Spectral scan: The graph to the left is a scan of a set of the 4 different concentrations of Nicotinic Acid. It shows the peaks clearly in relation to absorbance and the differences due to Nicotinic Acid concentration. Peaks: 213 and 261 nm The certificate supplied with these CRMs shows the absorbance values for each peak when measured against the hydrochloric acid blank, at the two wavelengths. When measured in your spectrophotometer you can validate your transmittance/absorbance scale for accuracy and linearity using the set with 4 different concentrations and the blank. Far UV Linearity Assessment: By plotting concentration against absorption when measuring the set of 4 Nicotinic Acid cells against the blank, you can test your spectrophotometer for linearity through the Far UV range. 9

10 Metal on Quartz UV/Vis Absorbance/Linearity Certified Wavelengths: Metal on Quartz Filter for absorbance and linearity in the UV and Visible area of the spectrum. CRM complete with UKAS ISO/IEC accredited certificate of calibration 250, 280, 340, 360, 400, 465, 500, 546.1, 590 and 635 nm at Spectral Band Width of 5 nm or less. Metal coated quartz filter with coated side protected by an optically contacted quartz window. The filters are mounted in protective aluminum holders that will fit a standard instrument cell holder. Product The Starna RM-1Q3Q9Q is a direct equivalent to the NIST SRM 2031, with a standard range of Metal on Quartz absorbance standards for the UV/Visible area of the spectrum. All Starna Metal on Quartz Filters have an optically contacted Quartz Cover Plate to protect the metalised surface and facilitate easy cleaning. This was also a feature of the original NIST SRM The filters allow Absorbance and Linearity at the ten wavelengths across the range and the relative values are shown on the table below. They RM-1Q3Q9Q (SRM 2031 Equivalent) can be certified at wavelengths other than those shown above, on request. The filters are produced in our ISO 9001 accredited production facility, under ISO Guide 34 as a reference material producer. Calibration is carried out in our ISO/IEC accredited Calibration Laboratory. Available Metal on Quartz Filters: Part No. %T Transmittance Absorbance Q1 1% 2.00 A Q3 3% 1.52 A 1Q 10% 1.00 A 3Q 30% 0.52 A 5Q 50% 0.30 A 9Q 90% 0.06 A Suggestions for Use: The UV/Visible Metal on Quartz Filters have a relatively flat profile across all UV/Visible wavelengths. Normally three representative absorption filters would be chosen for any given application but the filters may be bought individually, in sets as shown below or any combination from the standard range. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the For expanded uncertainty budget and expected parameters see page 35 Ordering Information (Let us know what combination you require): Description absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. It is important to confirm that Metal on Quartz Filters are suitable for use with your instrument as they have a highly reflective surface and some instruments are sensitive to back reflection within the sample compartment. Part Number Metal on Quartz Reference, 30%, & Blank Holder RM-3Q Metal on Quartz Reference, 3%, 50%, & Blank Holder RM-Q35Q Metal on Quartz Reference, 10%, 30%, 90%, & Blank Holder RM-1Q3Q9Q (NIST 2031a) 10 Starna Scientific

11 Starna Green Broadband - Wavelength, Absorbance/Linearity Primary Usage: Certified Wavelengths: A mixed ultra stable dye solution permanently heat fusion sealed in quartz cells. CRM complete with UKAS ISO/IEC accredited certificate of calibration Testing wavelength accuracy, absorbance scale and linearity in UV/Vis region. 257, 416, and 630 nm with a SBW up to 12 nm Far UV quartz cell that has been permanently heat fusion sealed. Product The use of stable aqueous dye solutions is an established and well recognized method for the validation of the absorbance scale and linearity of a spectrophotometer. When prepared at the appropriate concentrations, these references have spectral characteristics that allow both the certification of the wavelength (257, 416, and 630 nm) and absorbance scale (0.25, 0.5, 0.75 or 1.0 A) in one discrete reference. Within the concentration range available, if the absorbance scale of the spectrophotometer is linear, the apparent absorbencies of a series of concentrations will be a linear function of concentration. The Starna Green reference set of proven stability is supplied with a Certificate of Calibration valid for 2 years by the Starna Calibration Laboratory, which is accredited by UKAS under ISO/IEC 17025, and ISO Guide 34. Description Starna Green, Blank, 25X concentration Starna Green, Blank, 50X concentration Starna Green, Blank, 75X concentration Starna Green, Blank, 100X concentration Starna Green, Blank, 4 concentrations Part Number RM-1SG RM-2SG RM-3SG RM-4SG RM-1SG2SG3SG4SG 11

12 Holmium Oxide - UV/Vis - Wavelength Reference Primary Usage: Holmium oxide (4%) in perchloric acid (10%) sealed in a quartz cell, CRM complete with UKAS ISO/IEC accredited certificate of calibration Verification of wavelength scale accuracy in both UV and Visible Certified Wavelengths: 241.0, 249.9, 278.2, 287.1, 333.5, 345.6, 361.4, 385.4, 416.1, 451.1, 467.8, 485.2, 536.5, nm Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2, and 3 nm. Other SBWs can be provided on request. Filled Far UV quartz cell that has been permanently heat fusion sealed Product Holmium has been used as a wavelength standard for many years in both a doped glass filter and in liquid format. Starna has extended the use by creating a filled and permanently heat fusion sealed far UV quartz cell which allows it to be used into the UV. Offering sharp, stable peaks over the range of 240 to 650 nm Holmium perchlorate is used to confirm that the wavelength scale of your instrument is within the manufacturer's tolerances for the actual wavelength being measured. The use of the liquid filled quartz cell ensures that the optical configuration method for quality control is exactly the same as for a normal analysis. Suggestions for Use: Holmium presents a wide range of sharp resolvable peaks, which are easily used to compare the wavelength indication on your spectrophotometer to the known peak. The first procedure should be to scan the cell over the usable range to assure that all peaks can be resolved. Adjust the slit width (SBW) if possible and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances of the wavelength readout. If not, a Customer Support engineer may Description be required to check or adjust your instrument. Holmium is measured against air as a blank makes no difference to the certified values. It is not recommended or good practise to evaluate absorbance using a wavelength reference. Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Holmium reference will enable the user to build a data log of the instrument s spectral accuracy for use with certification and troubleshooting should the correlation change over time. Part Number Holmium, Wavelength reference cell RM-HL 12 Starna Scientific

13 Didymium Oxide - Visible - Wavelength Reference Primary Usage: Verification of wavelength scale accuracy in the Visible spectrum Certified Wavelengths: 298.0, 329.0, 354.0, 444.1, 468.7, 481.8, 512.0, 521.3, 574.9, 731.8, 740.1, 794.1, 801.2, nm Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2 and 3 nm. Other SBWs can be provided on request. Product Didymium Oxide in perchloric acid, sealed in a quartz cell, CRM complete with UKAS ISO/IEC accredited certificate of calibration Filled far UV quartz cell that has been permanently heat fusion sealed Didymium is a combination of two chemical elements, Praseodymium and Neodymium and has been used as a visible wavelength standard in the form of doped Glass Filters. Starna provides a quartz cell filled and permanently heat fusion sealed with a Didymium Perchloric solution that will facilitate more effectively the evaluation of your instrument. Didymium offers sharp, stable peaks over the range of 290 to 870 nm. The primary use of Didymium is to confirm that the wavelength scale of your instrument is within the manufacturer's tolerances for the actual wavelength being measured. The use of the liquid filled quartz cell ensures that the optical configuration method for quality control is exactly the same as for a normal analysis. Suggestions for Use: Didymium presents a wide range of sharp resolvable peaks, which are easily used to compare the wavelength indication on your spectrophotometer to the known peak. The first procedure should be to scan the cell over the usable range to assure that all peaks can be resolved. Adjust the slit width (SBW) if possible and scan rate, to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances of the wavelength readout. If not, a Customer Support engineer may be required to check or adjust your instrument. Didymium is measured against air as a blank makes no difference to the certified values. It is not recommended or good practise to evaluate absorbance using a wavelength reference. Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Didymium reference will enable the user to build a data log of the instrument s spectral accuracy for use with certification and troubleshooting should the correlation change over time Description Part Number Didymium, Wavelength reference cell RM-DL 13

14 Samarium Oxide - UV/Vis - Wavelength Primary Usage: Verification of wavelength accuracy in both UV/Visible, resolving complex peaks Certified Wavelengths: 235.2, 266.7, 279.1, 280.2, 305.3, 317.6, 331.7, 344.6, 401.5, , nm Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2 and 3 nm. Other SBWs can be provided on request. Product Samarium Oxide in perchloric acid, sealed in a quartz cell, CRM complete with UKAS ISO/IEC accredited certificate of calibration Far UV quartz cell that has been permanently sealed by heat fusion Samarium Oxide in a perchlorate solution is a particularly good reference for establishing wavelength calibration because it has useful peaks from 230 to 560 nm. Many peaks have a natural bandwidth of less than 5 nm permitting precise location of the peaks. This offers a distinct advantage over Glass Filters, which may have natural bandwidths greater than 30 nm. In addition to offering wavelength calibration, the Samarium oxide can be used to check instrument spectral bandwidth. Using peaks in the nm or the nm region, the instrument capability to resolve complex peaks can be tested. Suggestions for Use: Samarium presents a wide range of sharp resolvable peaks, which are easily used to compare the wavelength indication on your spectrophotometer to the known peak. The first procedure should be to scan the cell over the usable range to assure that all peaks can be resolved. Adjust the slit width (SBW) if possible and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances of the wavelength readout. If not, a Customer Support engineer may be required to check or adjust your instrument. Samarium is measured against air as a blank makes no difference to the certified values. It is not recommended or good practise to evaluate absorbance using a wavelength reference. Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Samarium reference will enable the user to build a data log of the instrument s spectral accuracy for use with certification and troubleshooting should the correlation change over time. Description Samarium Oxide, Wavelength reference Part Number RM-SL 14 Starna Scientific

15 Rare Earth Oxide - Far UV - Wavelength Reference Primary Usage: Certified Wavelengths: Verification of the Far UV wavelength scale of spectrophotometers 201, 212, 223, 240, 253 nm Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2 and 3 nm. Other SBWs can be provided on request. Rare Earth Oxide (0.05%) in sulfuric acid (0.03%) sealed in a quartz cell, CRM complete with UKAS ISO/IEC accredited certificate of calibration Filled far UV quartz cell that has been permanently heat sealed Product The validation of the wavelength scale in the Far UV is difficult because of the lack of suitable reference materials. Starna has developed a new Rare Earth reference material which allows wavelength scale validation down to 200 nm. The reference consists of a solution of the Rare Earth material dissolved in an aqueous solution of very dilute sulfuric acid, which has been permanently heat fusion sealed into a high quality quartz cuvette. The use of the liquid filled quartz cell ensures that the optical configuration method for quality control is exactly the same as for a normal analysis. Each reference set is supplied with an ISO/IEC certificate, which indicates the expected wavelength of each of five peaks and the confidence limit of the analysis. Suggestions for Use: This Rare Earth reference presents a wide range of resolvable peaks, which are easy to compare with the wavelength indication on your spectrophotometer. The first procedure should be to scan the Rare Earth reference cell over the usable range to ensure that all of the peaks can be resolved. Adjust the slit width and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances for the wavelength of your spectrophotometer. If not, a service technician may be required to check or adjust your instrument. Rare Earth is measured against air as a blank makes no difference to the certified values. It is not recommended or good practise to evaluate absorbance using a wavelength reference. Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Rare Earth reference will enable the user to build a data log of the instrument s spectral accuracy in the Far UV for use with certification and troubleshooting should the correlation change over time. Description Far UV Wavelength Reference Part Number RM-RE 15

16 Metal on Quartz Near Infra Red (NIR) Absorbance/Linearity Certified Wavelengths: ISO Accredited: Metal on Quartz Filter for absorbance/linearity in the Near IR area of the spectrum. CRM complete with UKAS ISO/IEC accredited certificate of calibration , 360.0, 465.0, 546.1, 635.0, 1100, 1700, 2210, 2500, 2800 nm Metal coated quartz filter with coated side protected by an optically contacted quartz window. The filters are mounted in protective aluminum holders that will fit a standard instrument cell holder. ISO/IEC 17025, ISO Guide 34 by UKAS & ISO 9001 by BSI. Product A standard range of Metal on Quartz absorbance standards for the NIR area of the spectrum to facilitate full compliance with USP requirements. All Starna Metal on Quartz Filters have an optically contacted quartz cover plate to protect the metalised surface and facilitate easy cleaning. The filters allow absorbance and linearity at certified wavelengths across the range from 250 nm to 2800 nm and the relative values are shown on the table to the right. They can be certified at wavelengths other than those shown above, on request. The filters are produced in our ISO 9001 accredited production facility under ISO Guide 34 as a reference material producer and calibrated in our ISO/ IEC accredited Calibration Laboratory. Suggestions for Use: RM-1NQ3NQ9NQ The NIR Metal on Quartz Filters are intended for use across a broad spectrum including the NIR with a relatively flat transmission profile across all wavelengths. Normally three representative absorption filters would be chosen for any given application but, the filters may be bought individually, in sets as shown below or any combination from the standard range. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty For expanded uncertainty budget and expected parameters see page 35 Available Metal on Quartz NIR Filters: Part No. %T Transmittance Absorbance NQ1 1% 2.00 A NQ3 3% 1.52 A 1NQ 10% 1.00 A 3NQ 30% 0.52 A 5NQ 50% 0.30 A 9NQ 90% 0.06 A budget of the certified references into consideration, if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. It is important to confirm that Metal on Quartz Filters are suitable for use with your instrument as they have a highly reflective surface and some instruments are sensitive to the positioning of such materials in the sample holder. Ordering Information (Let us know what combinations you require): Description Metal on Quartz NIR Reference, 30%, & Blank Holder Metal on Quartz NIR Reference, 3%, 50%, & Blank Holder Metal on Quartz NIR Reference, 1%, 3%, 50%, Blank Holder Part Number RM-3NQ RM-NQ35NQ RM-NQ1NQ35NQ 16 Starna Scientific

17 Near Infrared - Wavelength Reference Primary Usage: Quartz cell in either Transmittance or Transmittance/Transflectance format. CRM complete with UKAS ISO/IEC accredited certificate of calibration Verification of wavelength scale accuracy in the NIR spectrum Certified Wavelengths: 990.7, , , , , , , , , , , , nm Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2 and 3 nm. Other SBWs can be provided on request. Filled near IR quartz cell that has been permanently heat sealed Product The reference is used to validate the wavelength scale of a NIR spectrophotometer. With 13 certified peaks from 990 to 2537 nm that include some complex for instrument resolution evaluation. The reference is available in two formats: Transmittance: - 10mm optical pathlength with two clear windows (opposite windows to allow light to pass through the cell). Transmittance/Transflectance - 5mm or 10mm optical path length, depending on orientation. In 5mm configuration, the rear window is mirror coated to provide reflectance optical return so pathlength is effectively 10mm ( 2 x 5mm = 10mm). Each reference set is supplied with full certification and traceability to NIST through SRM The reference material is produced and certified in our ISO Guide 34 and ISO/IEC accredited laboratory. The reference can also be recertified as required. Resolvable Peaks nm nm nm nm nm nm nm nm nm nm nm nm nm Transmittance Transmittance/Transflectance Description NIR Wavelength Reference, Transmittance, NIR Wavelength Reference, Transmittance and Transflectance, Part Number RM-NIR RM-NIR/T 17

18 Stray Light Reference Materials Primary Usage: Usable Range: Materials with sharp cutoffs in transmission at specified wavelengths, CRM complete with UKAS ISO/IEC accredited certificate of calibration Detection of stray light in the UV and Visible 200 nm to 390 nm, depending on the material Filled far UV quartz cell that has been permanently heat fusion sealed Product Stray light is an indication of light transmitted through the sample, when there should not be light being transmitted. The presence of more stray light than specified in the instrument operator's manual may cause errors in your analysis. Stray light can be a problem over any wavelength range but the problem increases the further into the UV that instrument measurements are taken. The stray light reference materials are intended to determine the presence of stray light in your instrument. Each material stops the transmission of light below a specified wavelength. Any indication of light transmission below the specified cutoff wavelength by definition, must be stray light. Testing for stray light is important even if the spectrophotometer is not used below 390 nm, because it is an excellent indication of the overall health of the instrument optics, grating and deuterium lamp. Stray light determinations are run against a water blank supplied with all NIST traceable stray light references. The stray light calibration certificate is measured against the water blank supplied with the reference cell. When there are more than one stray light references in a set, they are all calibrated against a single stray light blank cell. Material: Cutoff: Concentration: Sodium Nitrite 390 nm 5% aqueous Potassium Iodide 260 nm 1% aqueous Sodium Iodide 260 nm 1% aqueous Lithium Carbonate 227 nm saturated Sodium Chloride 205 nm 1% aqueous Potassium Chloride 200 nm 1.2% aqueous Acetone 326 nm Spectroscopy grade Suggestions for Use: The procedure for using the stray light references is similar for all materials. Set your spectrophotometer s wavelength 20 nm above the cutoff for the stray light reference that you are using (for Potassium Iodide you would start at 280 nm). Insert the stray light reference cell in the measurement cell holder and the stray light blank cell in the blank cell holder of your instrument. Scan down into the UV to the lowest wavelength that your instrument manual specifies. Any light transmitted Description Sodium Nitrite & Blank Cell Potassium Iodide & Blank Cell Sodium Iodide & Blank Cell Lithium Carbonate & Blank Cell Potassium Chloride & Blank Cell Sodium Chloride & Blank Cell Acetone below the cutoff wavelength will be stray light. If the amount of stray light is greater than the specification given in your instrument manual, a service technician may be required to investigate and correct the problem. Periodically rescan with the same instrument configuration and compare the results. Over time you will have a data trail for your instrument which will make the detection and correction of any problems relating to stray light much more reliable. Part Number RM-SN RM-KI RM-SI RM-LC RM-KC RM-SC RM-AC 18 Starna Scientific

19 Stray Light Reference Materials Measured Absorbance (A) 19

20 Benzene Vapor for Spectral Resolution Primary Usage: Usable Range: 0.1ml Benzene in a vapor state sealed in a quartz cell Test the resolution at various bandwidths of grating based instruments in the UV 270 nm to 230 nm Far UV quartz cell that has been permanently heat fusion sealed Product The ability of your spectrophotometer to resolve absorption peaks is the basis of its theory of operation. If absorption peaks cannot be resolved adequately, then interfering peaks will be integrated with the correct peaks, thus giving an inaccurate measurement. Benzene vapor is an effective material for the detection of a grating based spectrophotometer s resolving power, as it has a great number of close but distinct absorption peaks. Benzene vapor may not work well with a photodiode array spectrophotometer. Suggestions for Use: Set your spectrophotometer to a Spectral Band Width (SBW) and a scan rate and note the settings. Scan from 240 to 265 nm and compare the peaks to the two charts and determine what changes need to be made to the setting of your spectrophotometer to improve the resolution of the scan. Continue to alter the slit width until you have optimized the resolution. Make a note of the settings in your quality procedures. Each time that the scan is repeated you can compare the new scan with previous scans to check for any variance. If your instrument needs service because of a drop in resolution, the historical data will greatly assist the service technician. Description Part Number Instrument Resolution reference cell, Benzene Vapor RM-BZ 20 Starna Scientific

21 Ultraviolet Bandwidth- Toluene in Hexane Primary Usage: Usable Range: Quartz cell filled with Toluene in Hexane and permanently heat fusion sealed, CRM complete with UKAS ISO/IEC accredited certificate of calibration Determination of bandwidth and resolution in the UV region nm and nm Far UV quartz cells that have been permanently sealed by heat fusion Product The Spectral Bandwidth (SBW) of a spectrophotometer is the basis of establishing its ability to resolve spectral features separated by small differences in wavelength. If a sample to be measured has an absorption peak at nm and there is an interfering peak at nm then it should be established whether or the spectrophotometer is able to resolve the two peaks separately or mixes the two absorption peaks into a single peak, leading to erroneous results. Toluene in Hexane is used as a reference for the calculation of SBW. The ratio of the transmittance/absorbance of the solution when read at two peak wavelengths (~268.7 and ~267.0 nm) relates directly to the SBW of the instrument being assessed. Regular use of this technique will assure that the resolution of your instrument is within the required range for your work. The Toluene in Hexane reference consists of two far UV quartz spectrophotometer cells with the solution permanently sealed by heat fusion in the cells. One cell contains 0.02% Toluene in Hexane, the other is a Hexane only blank. The Toluene in Hexane cell is traceable to SRM 935a which is a transmittance/ absorbance reference material. Table of Approximate Ratios Ratio: SBW: Description Part Number Toluene in Hexane cell with Hexane blank RM-TX 21

22 Neutral Density Filters Primary Usage: Certified Wavelengths: Neutral Density Glass Filters for absorbance/linearity in the Visible area of the spectrum. CRM complete with UKAS ISO/IEC accredited certificate of calibration Validation of the visible of photometric scale of spectrophotometers , 465.0, 546.1, 590.0, nm Glass Filters mounted in anodized aluminum holder Product The Starna neutral density filter sets are based on and traceable to the NIST sets SRM 930e, SRM 1930 and SRM These sets are used to test the photometric scale in both Transmission and Absorbance in the visible wavelength range. The filters are made from Schott NG-type glass, which block a known percentage of the light passing through it. We calibrate the glass type and thickness of the filters to produce known transmittance and absorbance values. The approximate values are shown on the chart below. Each NIST traceable filter is individually tested and certified. The neutral density filters are mounted in a black anodised aluminum holder, 12.5 x 12.5 x 45 mm, which is fully compatible with all standard spectro pho tom e ter cell hold ers. The filters have been shown most efficient in instruments with an effective bandwidth of 2.2 nm to 6.5 nm. Set RM-1N2N3N This set is identical to NIST SRM 930e and consists of three filters and a blank holder. Approximate values are: %T Transmittance Absorbance 10% 1.10 A 20% 0.78 A 30% 0.56 A Set RM-N1N35N This set is identical to NIST SRM 1930 and consists of three filters and a blank holder. Approximate values are: %T Transmittance Absorbance 1% 2.10 A 3% 1.60 A 50% 0.30 A Set RM-D1D39N This set is identical to NIST SRM 2930 and consists of three filters and a blank holder. Approximate values are: %T Transmittance Absorbance 0.1% 3.00 A 0.3% 2.50 A 92% 0.04 A Suggestions for Use: Set your spectrophotometer in either absorbance or transmission mode at nm. Using the empty filter holder (blank) zero the instrument. Insert each filter in turn and note the value. Set your instrument to each successive wavelength and repeat the procedure. The value that you read should be close to the measured certificate value listed for that filter. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. For expanded uncertainty budget and expected parameters see page 35 These readings from your instrument may also be used to plot a graph of the instrument linearity. The user may also build a data log of the instrument s spectral photometric accuracy. This can be used for certification and troubleshooting should the correlation change over time. If there is any discrepancy in the readings, a service technician can use the data collected to diagnose and correct any problems with your instrument. Description Neutral Density Filter set, 10, 20, 30 %T, & Blank Holder Neutral Density Filter set, 1, 3, 50 %T, & Blank Holder Neutral Density Filter set, 0.1, 0.3, 92 %T, & Blank Holder Part Number RM-1N2N3N RM-N1N35N RM-D1D39N 22 Starna Scientific

23 Visible References - Neutral Density Filters Absorbance Wavelength in nanometers 23

24 Glass Wavelength Filter - Holmium, Didymium Primary Usage: Usable Range: Verification of wavelength scale accuracy in both UV and Visible 241 nm to 640 nm (Holmium for UV/Vis). 430 nm to 890 nm (Didymium for Visible) Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.50, 1, 1.5, 2, and 3 nm. Other SBWs can be provided on request. Product Both Holmium and Didymium provide distinctive peaks that make each suitable for use as a wavelength standard. These robust Starna filters are more suitable for some environments than the liquid cells. They are mounted in an anodized aluminum housing to protect the polished surfaces. The Holmium and Didymium Glass Filters are used to ensure that the instrument wavelength scale is within the expected tolerances for the actual wavelength being measured. Holmium glass filter and Didymium glass filter, CRM complete with UKAS ISO/IEC accredited certificate of calibration Stress free glass filter mounted in an anodized aluminium holder Suggestions for Use: Holmium and Didymium filters present a wide range of peaks that can be resolved. The number of peaks resolved will depend on the spectral bandwidth used. The wavelength peaks can then be used to compare the wavelength indicated by your spectrophotometer to the known peak. The initial procedure should be to scan the filter over the usable range to identify all the peaks that can be resolved at the specified SBW of the instrument. Adjust the slit width (SBW)and scan rate to produce a usable spectra. Check each peak to ensure that the reading on your spectrophotometer is Description Holmium Glass, Wavelength reference filter Didymium Glass, Wavelength reference filter within the manufacturer's tolerances of the wavelength readout. If not, a service technician may be required to check or adjust your instrument. The filters should be measured against an air blank. Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Holmium or Didymium reference will enable the user to build a data log of the instrument s spectral accuracy for use with certification and troubleshooting should the correlation change over time. Part Number RM-HG RM-DG 24 Starna Scientific

25 RM-1N3N5DHG - Photometric Scale / Wavelength Primary Usage: Usable Range: Neutral Density Filter Set plus Holmium Glass Filter, CRM complete with UKAS ISO/IEC accredited certificate of calibration Validation of the visible of photometric scale and the UV/Vis wavelength scale to nm (1N3N5D); 241 to 640 nm (Holmium for UV/Vis) Glass Filters mounted in anodized aluminum holder Product The elements of this set include neutral density filters that are used to test the photometric scale in both Transmission and Absorbance in the visible wavelength range. The filters are made from Schott NG-type glass which absorbs a known percentage of the light passing through it. Adding the Holmium glass filter to the set facilitates validation of the wavelength scale by utilizing the distinctive peaks of the Holmium filter. The thickness of the filters is calculated to produce known transmission and absorption values. The approximate values are shown on the chart. Each NIST traceable filter is individually tested and certified. The neutral density and Holmium filters are mounted in black anodized aluminum holders, 12.5 x 12.5 x 45 mm, which is fully compatible with all standard spec tro pho tom e ter cell hold ers. The filters have been shown to be effective in instruments with bandwidths of 2.2 nm to 6.5 nm. Set RM-1N3N5DHG This set consists of 3 neutral density filters, a blank holder and a Holmium Glass filter. Neutral Density filter's Approximate values are: %T Transmittance Absorbance 10% 1.00 A 30% 0.50 A 52% 0.25 A The Holmium Glass filter is certified at 11 distinctive peaks ranging from 241 to 640. The seven certified Spectral Band Widths (SBW) measured are 0.10, 0.25, 0.50, 1.00, 1.50, 2.00, and 3.00 nm. Suggestions for Use: Set your spectrophotometer in either absorbance or transmission at nm. Using the empty filter holder (blank) zero the instrument. Insert each filter in turn and note the value. Set your instrument to each successive wavelength and repeat the procedure. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. These readings can be plotted to check the instrument linearity. For wavelength validation the procedure should be to scan the For expanded uncertainty budget and expected parameters see page 35 Holmium wavelength reference filter over the usable range to ensure that all of the peaks can be resolved. Adjust the slit width (SBW) and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances for the wavelength of your spectrophotometer. Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Neutral Density and Holmium references will enable the user to build a data log of the instrument s spectral accuracy for use with validation and troubleshooting should the correlation change over time Description Neutral Density & Wavelength Filter set Part Number RM-1N3N5DHG 25

26 Microplate Reader Visible Reference Plate Primary Usage: Certified Wavelengths: 96 well microplate validation filters for wavelength and absorbance scales, CRM complete with UKAS ISO/IEC accredited certificate of calibration Validation of the visible of photometric scale of 96 well microplate readers. Wavelength: Neutral Density , 465.0, 546.1, 590.0, nm. Holmium - 360, 418, 445, 453, 460, 536, 637 nm, Absorbance scale: 440 nm to 635 nm Glass Filters mounted in 96 well format anodized aluminum holder Product Our 96 well microplate reader filter sets are based on and traceable to the NIST sets SRM 930e, SRM 1930 and NIST This microplate is used to test the photometric scale in both Transmission and Absorbance and wavelength accuracy in the visible wavelength range. The absorbance filters are made from Schott NG-type glass, which absorbs a known percentage of the light passing through it. The thickness of the particular glass type used is calculated to produce filters of approximate transmittance/absorbance values, these approximate values are shown on the chart below. Each NIST traceable filter is individually tested and certified. The wavelength filter is made of Holmium glass and is effective across the visible range. The filters have been shown to be effective in all microplate readers on the market. Specifications: Certified Wavelengths: Neutral Density Absorbance Filters: nm nm nm nm nm Holmium Wavelength Filter (approximate wavelengths): 637 nm 536 nm 460 nm 453 nm 445 nm 418 nm 360 nm Filters in reference plate: Configuration of Reference Plate: Well Positions Filter Type Filter Values C2 - H2 Air Blank C4 - H4 Neutral Density 30%T (0.5 Absorbance) C6 - H6 Neutral Density 10%T (1.0 Absorbance) C8 - H8 Neutral Density 3%T (1.5 Absorbance) C10 - H10 Neutral Density 1%T (2.0 Absorbance) C12 - H12 Holmium Peaks from 360 to 637 nm Description Part Number 96 Well Microplate Reader Validation Plate RMMR-1N3NN1N3HG 26 Starna Scientific

27 Microplate Reader UV Reference Plate 96 well microplate validation filters for wavelength and absorbance scales, CRM complete with UKAS ISO/IEC accredited certificate of calibration Primary Usage: Certified Wavelengths: Product Validation of the UV wavelength and photometric scales of 96 well microplate readers. Wavelength: Potassium Dichromate , 257.0, 313.0, nm. Absorbance scale: 0-2 A. Holmium liquid 241.0, 249.9, 278.2, 287.1, 333.5, 345.6, 361.4, 385.4, 416.1, 451.1, 467.8, 485.2, 536.5, nm. Liquid reference (patented cell design) mounted in 96 well format anodized aluminum holder Our 96 well microplate reader liquid UV sets are based on and traceable to the NIST CRMs, SRM 935e, and NIST This microplate is used to test the photometric scale in both Transmittance and Absorbance and wavelength accuracy in the UV wavelength range. All the liquid filled references are permanently heat fused sealed in 5.0 mm path length, Far UV quartz cells, each containing a patented bubble trap. The absorbance/transmittance references are potassium dichromate solutions, with the concentration adjusted to match the 5.0 mm cell path length. Each NIST traceable CRM is individually tested and certified. The wavelength reference is Holmium oxide liquid and is effective across the UV/visible range. The references have been shown to be effective in all microplate readers on the market. Specifications: Certified Wavelengths: Potassium Dichromate Absorbance/Transmittance References: nm nm nm nm Holmium Wavelength: 241.0, 249.9, 278.2, 287.1, 333.5, 345.6, 361.4, 385.4, 416.1, 451.1, 467.8, 485.2, 536.5, nm Configuration of Reference Plate: Well Positions Reference Type Reference Values C2 - H2 C4 - H4 C6 - H6 C8 - H8 C10 - H10 C12 - H12 Absorbance/Transmittance Absorbance/Transmittance Absorbance/Transmittance Absorbance/Transmittance Absorbance/Transmittance Wavelength 0.001M Perchloric acid Blank Potassium Dichromate (40 mg/l) mm path length Potassium Dichromate (80 mg/l) mm path length Potassium Dichromate (120 mg/l) mm path length Potassium Dichromate (160 mg/l) mm path length Holmium Peaks from 240 to 650 nm Description Part Number 96 Well Microplate Reader UV Validation Plate RMMR HL 27

28 260/280 nm Validation Reference for DNA and RNA Primary Usage: Certified Wavelengths: Standard solution which gives a stable 260/280 nm ratio of 1.8 to 2.0, CRM complete with UKAS ISO/IEC accredited certificate of calibration Control the quality of DNA/RNA purity analysis 260 nm and 280 nm Far UV quartz cell that has been permanently heat fusion sealed or vials with a crimp cap or screw cap Product When running DNA or RNA purity analysis on your spectrophotometer, it is important to ensure that your instrument is working correctly. Our DNA/RNA validation standard in a permanently sealed quartz cell or vial (crimp or screw cap) contains a stable solution, which mimics the 260/280 nm ratio of DNA and RNA. The volume of each vial is 1.5 ml. The RM-DNA (permanently sealed reference) is supplied with a certificate which lists the expected 260/280 nm ratio of the cell Suggestions for Use: The importance of this reference is that it allows you to validate your work in real time. When measuring unknown samples you can assure that your instrument is working correctly by reading the validation standard both before, during and after each run of unknowns. If you are using a spectrophotometer which automatically calculates the 260/280 ratio, then all that you need to do is place the DNA/RNA validation reference in your spectrophotometer and run the instrument's routine for the 260/280 ratio. If the ratio is valid when compared to the certificate then you can be assured that your instrument is calculating the ratio correctly. In a spectrophotometer without an automatic calculation feature, simply use the DNA/RNA validation reference as you would your other samples to validate your analytical procedure. Description Permanently heat fusion sealed into a cuvette 5X concentration, 1 Crimp Cap Vial 5X concentration, 2 Crimp Cap Vials 5X concentration, 4 Crimp Cap Vials 5X concentration, 1 Screw Cap Vial 5X concentration, 2 Screw Cap Vials 5X concentration, 4 Screw Cap Vials and the confidence limit of the ratio. The validation analysis is performed by our ISO/IEC accredited laboratory and is traceable to NIST. The vials with caps (both crimp cap and screw cap) reseal to maintain the integrity of the DNACON. This format allows for the removal of the DNACON by pipet or syringe. The vials are available in concentrations of 5x and 10x. The number of vials and the concentrations are listed below. Generally, the 5x concentration is suitable for the NanoDrop instrument while the 10x concentration is suitable for micro drop systems such as Jenway/Bibby. Additionally, at 260 nm, results are approximately as follows: Light Path 5X Concentration 10X Concentration 0.2mm 0.1 A 0.2 A 0.5mm 0.25 A 0.5 A 1mm 0.5 A 1 A 10mm 5 A 10 A Part Number RM-DNA DNACON5X-CC1 DNACON5X-CC2 DNACON5X-CC4 DNACON5X-SC1 DNACON5X-SC2 DNACON5X-SC4 10X concentration, 1 Crimp Cap Vial DNACON10X-CC1 10X concentration, 2 Crimp Cap Vials DNACON10X-CC2 10X concentration, 4 Crimp Cap Vials DNACON10X-CC4 10X concentration, 1 Screw Cap Vial DNACON10X-SC1 10X concentration, 2 Screw Cap Vials DNACON10X-SC2 10X concentration, 4 Screw Cap Vials DNACON10X-SC4 28 Starna Scientific

29 260/280 nm Validation Reference for DNA and RNA RM-DNA Screw Cap DNACON 1.5 ml Vials Crimp Cap Typical Uncorrected Absorbance Values: 260 nm 0.765A 280 nm 0.405A Ratio:

30 Quinine Sulfate Fluorescence Reference Primary Usage: Usable Range: A solution of 1mg/L Quinine Sulfate in mol/l Perchloric Acid. The reference material is permanently heat fusion sealed in quartz fluorometer cells, CRM complete with UKAS ISO/IEC accredited certificate of calibration. Calibration and evaluation of methods used on a fluorescence spectrophotometer. 375 nm to 675 nm Far UV quartz fluorescence cell that has been permanently heat fusion sealed. The reference is also available with a blank if required. Product NIST SRM 936a is supplied as a crystalline material and is made into a solution as described in the Certificate of Analysis supplied with NIST SRM 936a. Starna then fills a high quality far UV quartz fluorometer cell with the SRM solution and permanently heat fusion seals the cell. The reference is designed for multiple use over time and for inter instrument comparisons. Quinine Sulfate references can also be supplied with a perchloric acid blank. Where instrument linearity needs to be established, sets with different concentrations of Quinine Sulphate and a blank are available in the following concentrations, 0.25, 0.5, 0.75 & 1.0 mg/l. The Starna reference set is supplied with a Certificate of Validation by our Calibration Laboratory which is accredited by UKAS under ISO 9001, ISO/IEC 17025, and ISO Guide 34. The certificate is valid for a period of two years. Corrected molecular emission spectrum Linearity Regression Coefficient Description Quinine Sulfate Reference (1 concentration 1.0 mg/l, without blank) Quinine Sulfate Reference (1 concentration 1.0 mg/l & blank) Quinine Sulfate Reference (2 concentrations, & blank) Quinine Sulfate Reference (3 concentrations, & blank) Quinine Sulfate Reference (4 concentrations, & blank) Part Number RM-QS RM-QS00 RM-2QS00 RM-3QS00 RM-4QS00 30 Starna Scientific

31 Raman Reference, very high signal to noise ratio Primary Usage: Usable Range: ISO/IEC Accreditation: Ultra-high pure water, permanently heat fusion sealed in a high quality quartz fluorometer cell. Evaluation of sensitivity in the qualification of fluorescence spectrophotometers specifically the instrument Signal to Noise Ratio near the detection limits using the Raman band emission at specific wavelengths. 250 to 600 nm Far UV quartz fluorescence cell that has been permanently heat fusion sealed. Produced and manufactured under ISO/IEC 17025, ISO Guide 34 by UKAS, protocols Product With years of development, Starna has perfected the technique of producing Ultra Pure water and permanently heat fusion sealing the water into a high quality Far UV quartz fluorometer cell. The reference can be used multiple times on multiple instruments because the material does not deteriorate. The Starna reference with a Certificate of Validation by our reference lab which is accredited by UKAS under ISO 9001, ISO/IEC 17025, and ISO Guide 34. Molecular Emission Spectrum Raman Water Sensitivity Excitation: 350 nm Emission Peak: 398 nm Molecular Emission Spectrum Raman Water Sensitivity Excitation: 500 nm Emission Peak: nm Description Ultra-pure Water Reference Part Number RM-H2O 31

32 Sets Recommended For Instrument Qualification To make instrument Qualification easier, we have assembled reference materials into sets which allow you to meet specific verification requirements. It is more cost effective to purchase a group of references in a set instead of purchasing several references individually. The following list of sets will allow you to meet the qualifications of the European Pharmacopoeia, the United States Pharmacopeia and sets that will satisfy the requirements of both the EP and USP. European Pharmacopoeia: Part Number: RM-0660HLKCTX This set is used to meet European Pharmacopoeia requirements and contains: Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 60 mg/l Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Wavelength Accuracy 240 nm to 650 nm Stray Light blank Stray Light 200 nm to 400 nm Potassium Chloride, blank Stray Light 198 nm Toluene in Hexane Resolution 267 nm to nm European Pharmacopoeia and USP: Part Number: RM-0660HLKCSITX This set meets European Pharmacopoeia requirements as well as the United States Pharmacopeia requirements and contains: Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 60 mg/l Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 600mg/L Photometric Accuracy 430 nm Holmium Oxide Wavelength Accuracy 240 nm to 650 nm Stray Light blank Stray Light 200 nm to 400 nm Potassium Chloride Stray Light 198 nm Sodium Iodide Stray Light 220 nm Toluene in Hexane Resolution 267 nm to nm Professional & Support Services Set for all Pharmacopoeias and USP: Part Number: RM-0660HLKCSISNTX This set meets European Pharmacopoeia requirements as well as the requirements of the United States Pharmacopeia. Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 60 mg/l Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Wavelength Accuracy 240 nm to 650 nm Stray Light blank Stray Light 200 nm to 400 nm Potassium Chloride Stray Light 198 nm Sodium Iodide Stray Light 220 nm Sodium Nitrate Stray Light 340 nm Toluene in Hexane Resolution 267 nm to nm 32 Starna Scientific

33 Sets Recommended for Instrument Qualification United States Pharmacopeia set: Part Number: RM-06HLKI This set is primarily used in North America due to it's similarity to NIST SRMs and contains: Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 60 mg/l Photometric Accuracy 235 nm to 350 nm Holmium Perchloric Wavelength Accuracy 240 nm to 650 nm Stray Light blank Stray Light 200 nm to 400 nm Potassium Iodide Stray Light 260 nm United States Pharmacopeia set with Didymium: Part Number: RM-06DLKI This set is used where wavelength verification is required at higher visible wavelengths and contains: Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 60 mg/l Photometric Accuracy 235 nm to 350 nm Didymium Perchloric Wavelength Accuracy 290 nm to 870 nm Stray Light blank Stray Light 200 nm to 400 nm Potassium Iodide Stray Light 260 nm United States Pharmacopeia set with linearity: Part Number: RM HLKI This set is used where absorbance linearity in the UV is required to be validated as well as the wavelength and stray light requirements of the USP. The set contains: Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 20, 60, 100 mg/l Photometric Accuracy 235 nm to 350 nm Holmium Perchloric Wavelength Accuracy 240 nm to 650 nm Potassium Iodide, blank Stray Light 260 nm UV Absorbance/Transmittance Scale & Linearity validation: Part Number: RM With five concentrations of Potassium Dichromate, used to test the photometric scale and linearity between 0.095A and 1.5A. Potassium Dichromate Blank Potassium Dichromate 20, 40, 60, 80, 100 mg/l Photometric Accuracy 235 nm to 350 nm Part Number: RM With six concentrations of Potassium Dichromate, used to test the photometric scale and linearity between 1.5A and 3.3A. Potassium Dichromate Blank Potassium Dichromate 120, 140, 160, 180, 220 & 240 mg/l Photometric Accuracy 235 nm to 350 nm Part Number: RM With six concentrations of Potassium Dichromate used to test the photometric scale and linearity between 0.19A to 3.3A. Potassium Dichromate Blank Potassium Dichromate 40, 80, 120, 160, 200 & 240 mg/l Photometric Accuracy 235 nm to 350 nm 33

34 Product Summary Part No. Photometric Scale Verification UV Potassium Dichromate 20 mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM mg/l RM-60 PDC 5 concentration set RM PDC 6 concentration set RM PDC 6 concentration set RM Photometric Scale Verification UV.VIS Metal on Quartz 1% & Blank RM-Q1 3% & Blank RM-Q3 10% & Blank RM-1Q 30% & Blank RM-3Q 50% & Blank RM-5Q 90% & Blank RM-9Q 3%, 50% & Blank RM-Q35Q 10%, 30%, 90% & Blank RM-1Q3Q9Q Starna Green 25X conc & Blank RM-1SG 50X conc & Blank RM-2SG 75X conc & Blank RM-3SG 100X conc & Blank RM-4SG 4 conc & Blank RM-1SG2SG3SG4SG Far UV Photometric Scale Verification Nicotinic Acid 6mg/L RM-1A 12mg/L RM-2A 18mg/L RM-3A 24mg/L RM-4A 4 concentration set RM-1A2A3A4A UV/VIS Wavelength Accuracy Holmium Glass filter RM-HG Didymium Glass filter RM-DG Holmium Oxide RM-HL Didymium Oxide RM-DL Samarium Oxide RM-SL Far UV Wavelength Accuracy Rare Earth RM-RE Stray Light Sodium Iodide Potassium Iodide Lithium Carbonate Potassium Chloride Sodium Chloride Sodium Nitrite Acetone Instrument Resolution Benzene Vapor RM-SI RM-KI RM-LC RM-KC RM-SC RM-SN RM-AC RM-BZ Part No. SBW Bandwidth Calibration Toluene in Hexane RM-TX Validation of 260/280 ratio DNACON Sealed Cell RM-DNA Crimp Cap Vial 1 Vial 5X concentration DNACON5X-CC1 2 Vials 5X concentration DNACON5X-CC2 4 Vials 5X concentration DNACON5X-CC4 1 Vial 10X concentration DNACON10X-CC1 2 Vials 10X concentration DNACON10X-CC2 4 Vials 10X concentration DNACON10X-CC4 Screw Cap Vial 1 Vial 5X concentration DNACON5X-SC1 2 Vials 5X concentration DNACON5X-SC2 4 Vials 5X concentration DNACON5X-SC4 1 Vial 10X concentration, DNACON10X-SC1 2 Vial 10X concentration, DNACON10X-SC2 4 Vial 10X concentration, DNACON10X-SC4 Absorbance scale & Linearity Visible Neutral Density Filter sets 10, 20, 30%T RM-1N2N3N 1, 3, 50%T RM-N1N35N 1, 0.3, 92%T RM-D1D39N Microplate Absorbance scale and Wavelength Accuracy Visible Microplate Reference RMMR-1N3NN1N3HG Microplate Cell Adaptor SCP-96-8R Wavelength Accuracy NIR NIR transmittance reference NIR transmittance/reflectance reference RM-NIR RM-NIR/T Photometric Scale Verification NIR Metal on Quartz 1% & Blank RM-NQ1 3% & Blank RM-NQ3 10% & Blank RM-1NQ 30% & Blank RM-3NQ 50% & Blank RM-5NQ 90% & Blank RM-9NQ 3%, 50% & Blank RM-NQ35NQ 10%, 30%, 90% & Blank RM-1NQ3NQ9NQ Fluorescent Reference Quinine Sulfate 1 x 1.0 mg/l, RM-QS 1 x1.0 mg/l & blank RM-QS00 2 concentrations, & blank RM-2QS00 3 concentrations, & blank RM-3QS00 4 concentrations, & blank RM-4QS00 Raman Reference, Very High Signal to Noise Ratio Ultra Pure Water RM-H20 34 Starna Scientific

35 Calibration Services & Certificates on USB Stick Calibration Services Certification on USB Stick The Starna Calibration Laboratory offers a calibration and re-calibration service to ISO/IEC 17025, the highest level of accreditation currently available, second only to National Metrology Institute laboratories. In addition to this calibration service being available for reference materials manufactured by Starna it is also available for many similar reference materials produced by third parties, including organisations such as NIST, Stranaska, as well as a number of other companies. Starna Cells is also able to extend the service to cover a wide range of optical products within the UV/VIS/NIR range, We welcome enquiries for all types of similar product, even though in some instances they may not have been produced in the first instance as certified references. Calibration Production Experience & Accreditation History Starna has over forty-five years experience in the production of both Glass Filters and permanently heat fusion sealed liquid Reference materials. Calibration accreditation through UKAS to ISO/IEC17025 was achieved in This was followed in 2006 by Starna also achieving ISO/IEC Guide 34 as a Certified Reference Material Producer. Calibration is carried out on any one of five fully qualified reference spectrophotometers. The above accreditation, experience and accumulated data puts Starna in a unique position with regard to Certified Reference Materials ranging from the Deep UV to the NIR The scope detailing specifically what the Starna accreditation covers, including uncertainty values, can be viewed at the following URL on the UKAS web site. Certification Provided in USB Electronic Format As a result of increased customer requests for certificates and associated data to be supplied in electronic format, especially from those customers working in controlled environments, in 2013 Starna changed certificates to an electronic format. This advancement in presentation has helped the efficiency of Pharmaceutical companies, Instrument Manufacturers and other customers worldwide, another example of Starna taking the lead in reference material presentation. This environmentally friendly approach allows greater customer flexibility in the use and circulation of Calibration Certificates, allowing data to be input directly into other databases. As part of the recalibration protocol certified references are measured as recieved, prior to any cleaning or other analysis. Many customers request the 'as received data' to be included when the sets are returned after recalibration, particularly where this is part of the customer ISO/IEC protocols. This data is a good "health" indicator relating to the environment in which the references have been used and handled. Starna has often been able to offer advice in their use and handling, which has saved customers from unnecessary expense by replacing references, when it could otherwise have been avoided. Expected Parameters and Expanded Uncertainty Budgets Guarantees Starna Reference Materials carry a lifetime Guarantee subject to: the references being re-calibrated by Starna at intervals not greater than two years; the references have not been subject to thermal, optical or physical abuse. Starna will replace free of charge any references that, under normal environmental conditions, move outside their expanded uncertainty budget (k=3), within a two year period and cannot be corrected by cleaning or any other process. The expanded uncertainty budget (k=2) is the tolerance that we could expect to measure in the assigned value on any one of our reference spectrophotometers during the 2 year period of certification. When used to verify the performance of any given spectrophotometer this certificate tolerance must be added in a simple linear manner to the stated appropriate specification accuracy of the instrument on the test, see following example: Wavelength Absorbance Certificate ±0.10 nm ± A Instrument ±0.30 nm ± A Total ±0.40 nm ± A 35