Applications of HTRF and Tag-lite Assays for HTP Antibody Screening

Size: px
Start display at page:

Download "Applications of HTRF and Tag-lite Assays for HTP Antibody Screening"

Transcription

1 Applications of HTRF and Tag-lite Assays for HTP Antibody Screening Brigitte Devaux, PhD Bristol Myers Squibb, Redwood City CA HTRF Symposium April 25,

2 Introduction Generate human therapeutic antibodies to large number of targets (soluble or membrane bound) for various therapeutic indications Also produce reagent antibodies for research/development/clinical applications Hybridoma technology Fusion of splenocytes with myeloma partner Need to develop HTP assays amenable to screening hybridoma supernatants from fusion plates Immunization Create potential for screening on multiple antigens or cell types Tagged antigen versus irrelevant Ag with same tag human/cyno/mouse Ag target Conjugated Ag versus un-conjugated Ag (with PEG for example) Homologues from same family Transfectants and cancer cells 2

3 Hybridoma Workflow Human Ig Tg mice spleens E-Fusion Splenocytes + fusion partner Balb/c spleens HuG/K Screening HTRF Automation-96 ELISA (soluble Ag) Hybridoma-96 HTRF (soluble Ag) AD-384 Antigen Screening FACS (cells) Hybridoma-96 FMAT/Tag-lite (cells) AD-384 Ag specific Hit Picking Automation-24 Rescreen of positive lines Subcloning Expansion Purification

4 HTP Antigen Screening Assays Requirements: Detect antibody binding to antigen (Soluble or Cell based) Robust & flexible assays No wash steps Work in conditioned media Sensitivity: Adequate for Ab concentration range from 10 ng-10 g /ml +/- response (no need for EC50 determination) Up to 10,000 samples in a run Time sensitive (24 hour turn around) Rapid assay development against a wide range of targets

5 Presentation Outline HTRF antigen screening assays (3 examples) HTRF with tagged antigens (e.g. His, Fc - Ag1 & 2) HTRF with non-tagged antigen (Ag3) and labeled secondary reagents (non-blocking polyclonal) HTRF with multiple labeled antigens in one well (duplexing, 2 examples) HTRF with Drug Ab IgG4 versus irrelevant IgG4 HTRF with PEG versus non PEG Ag HTRF Blocking assay (1 example) HTRF Tag-Lite (2 examples) Tag-lite with GPCR1 Tag-lite with GPCR2 5

6 Presentation Outline HTRF antigen screening assays (3 examples) HTRF with tagged antigens (Ag1 & 2) HTRF with non-tagged antigen (Ag3) and labeled secondary reagents (non-blocking polyclonal) HTRF with multiple labeled antigens in one well (duplexing, 2 examples) HTRF with Drug Ab IgG4 versus irrelevant IgG4 HTRF with PEG versus non PEG Ag HTRF Blocking assay (1 example) HTRF Tag-Lite (2 examples) Tag-lite with GPCR1 Tag-lite with GPCR2 6

7 HTRF 665 Ratio HTRF 665 Ratio HTRF with Tagged Antigens (His, Fc): Ag 1 Tb Streptavidin Tb Biotin anti-his Ab Ag1-His (monomer) Murine Ab in supernatant Anti-mouse Fc AlexaFluor 647 Tb Anti-mouse Fc Tb Murine Ab in Supernatant Ag1-Hu Fc (dimer) Anti-Human Fc D Ag1-His MAB 1 MAB 2 MAB 3 migg Ag 1 - Hu Fc MAB 1 MAB 2 MAB 3 migg Clone Conc. [ug/ml] Mab1 & 3: Positive Control Mab 2, Mouse IgG : Negative Control Clone Conc. [ug/ml]

8 AG1-His HTRF 665 Ratio HTRF Ag1-His Fusion Screening at 96 well stage Blue : Positive Control Red : Binding Supernatants Well Identification of Six Ab Supernatants with Binding Activity

9 Ag1-His HTRF 665 Ratio Ag1-His HTRF 665 Ratio Supernatants tested in Ag1 ELISA and FACS assays at 24 well stage Red : Identified by the HTRF assay at the 96 well stage Ag1-hFc ELISA (OD) CHO-Ag1 FACS MFI Same hits in HTRF and ELISA assays Not all HTRF/ELISA hits bind by FACS

10 HTRF with Tagged Antigens (His): Ag 2 Antigen has a large number of leucine rich repeats on its extracellular surface Primary screening: HTRF and ELISA in parallel HTRF binding in solution ELISA with Ag coated on plate HTRF Format Tb Anti-mouse Fc Tb mouse anti-his Ag2-His Human Clone in supernatant Anti-Human Fc AlexaFluor 647

11 Ag 2 - HTRF & ELISA vs. FACS All HTRF positive are FACS positives Good correlation between HTRF signal and FACS signal FACS positives (also HTRF +) missed by ELISA(OD<0.3)! Blue are FACS positive - subcloned

12 HTRF 665 Ratio HTRF with Labeled Secondary Reagent Detection: Ag3 Tb or Anti-mouse FC Tb Mouse Clone in Supernatant Ag3 Polyclonal anti-ag3 DL649 Polyclonal Biotinylated anti-ag3 and Streptavidin DL HTRF - Ag3 - Labeled vs Biotinylated Secondary Antibody Labeled (100 ng/ml Ag3) Labeled (20 ng/ml Ag3) Labeled (5 ng/ml Ag3) Biotinylated (100 ng/ml Ag3) to 25 1 to to to Biotinylated (20 ng/ml Ag3) Biotinylated (5 ng/ml Ag3) Positive Control Clone (Dilution) Direct and indirect labeling of polyclonal generated same results

13 Ag3 HTRF 650 Ratio Positive Control (1:500) Positive Control (1:10) Potential Hits in Red Negative Control

14 Presentation Outline HTRF antigen screening assays (3 examples) HTRF with tagged antigens (Ag1 & 2) HTRF with non-tagged antigen (Ag3) and labeled secondary reagents (non-blocking polyclonal) HTRF with multiple labeled antigens in one well (duplexing, 2 examples) HTRF with Drug Ab IgG4 versus irrelevant IgG4 HTRF with PEG versus non PEG Ag HTRF Blocking assay (1 example) HTRF Tag-Lite (2 examples) Tag-lite with GPCR1 Tag-lite with GPCR2 14

15 HTRF with multiple Labeled Antigens in one well Search for anti-id Abs The antigen is an Ab! Do not want Abs binding to Fc domain Duplexing: Drug Ab IgG4 2 assays in 1 Tb Anti-mouse Fc Tb Mouse Clone in Supernatant Drug Ab (IgG4) DL649 AND Irrelevant IgG4 DL488

16 665 Ratio Drug Ab IgG4 DL Ratio Yellow : Selected hits Light Blue : Positive Control Dark Blue or Pink : Not selected Immunogen : Drug Ab V domain only Ratio Immunogen : Drug Ab IgG Ratio Irrelevant IgG4 DL 488 More anti-ids obtained when immunizing with V part of the Ab

17 HTRF with PEG-conjugated Antigen versus non-peg Ag Duplexing: PEG-Ag versus non-peg Ag Positive Control Ab PEG & non-peg Ag selective Sups PEG-Ag Selective Sups Positive Control Ab Non-PEG Ag selective Sups PEG-Ag did not coat to plates by ELISA No problem with HTRF

18 Presentation Outline HTRF antigen screening assays (3 examples) HTRF with tagged antigens (Ag1 & 2) HTRF with non-tagged antigen (Ag3) and labeled secondary reagents (non-blocking polyclonal) HTRF with multiple labeled antigens in one well (duplexing, 2 examples) HTRF with Drug Ab IgG4 versus irrelevant IgG4 HTRF with PEG versus non PEG Ag HTRF Blocking assay (1 example) HTRF Tag-Lite (2 examples) Tag-lite with GPCR1 Tag-lite with GPCR2 18

19 HTRF Blocking assay Goal: identify anti-ids which bind to Ab when ligand is bound (non-blockers) or anti-ids which interfere with ligand binding (blockers) Tb Anti-mouse FC Tb Mouse Clone in Supernatant Irrelevant Ligand (In Excess) Drug Ab (IgG4) DL649 Or Tb Anti-mouse FC Tb Mouse Clone in Supernatant Ligand (In Excess) Irrelevant Ab (IgG4) DL649

20 21F3 23C2 11C2 8B5 14H10 16C11 3G4 15F7 27D5 13G6 15F8 22A10 21D2 22G10 38C1 38H2 42C5 42D9 49B3 55D9 4E9 17F9 18H2 25E5 Neg. control HTRF 665 Ratio HTRF Blocking Assay +/- Ligand HTRF Assay - Drug +/- Ligand - Anti-Id Ab Titration NB NB 8000 No Ligand - Clone at 5 ug/ml 6000 No Ligand - Clone at 1 ug/ml No Ligand - clone at 0.2 ug/ml ug/ml Ligand - Clone at 5 ug/ml 25 ug/ml Ligand - Clone at 1 ug/ml ug/ml Ligand - Clone at 0.2 ug/ml Preincubation ligand/drug Ab for 45 min Map Clone ID Most clones Most shown clones are are blockers Two Two non-blockers (anti-framework) (anti-framework)

21 HTRF: Conclusions Advantages Homogenous assay - No wash Sensitive (5ng/ml or less) Rapid (1 hr incubation time) Easily developed and automated Reagents stable Low volume 384 well plates Small reaction volumes Binding in solution Binding antigen to plates for ELISA may block a subset of epitopes Disadvantages Homogenous assay Hook effect Can make distinguishing supernatants based on single point signals difficult Requires antigen to be tagged or labeled May remove access to certain epitopes Matrix (conditioned media) can interfere with HTRF ratio Ideally positive control in media

22 Presentation Outline HTRF antigen screening assays (3 examples) HTRF with tagged antigens (Ag1 & 2) HTRF with non-tagged antigen (Ag3) and labeled secondary reagents (non-blocking polyclonal) HTRF with multiple labeled antigens in one well (duplexing, 2 examples) HTRF with Drug Ab IgG4 versus irrelevant IgG4 HTRF with PEG versus non PEG Ag HTRF Blocking assay (1 example) HTRF Tag-Lite (2 examples) Tag-lite with GPCR1 Tag-lite with GPCR2 22

23 Tag-lite Technology: Applied to two GPCR Targets Mix and Read Alexafluor labeled Secondary Ab Ligand or Ab binding to GPCR target Tb-Labeled Receptor F1 F2 HTRF signal when binding occurs Clip, Snap or Halo enzyme (20 kd)

24 HTRF 665 Ratio HTRF 665 Ratio HTRF 665 Ratio GPCR 1 HTRF Ligand & Ab Binding to Cells Tag-lite - Cell Comparison Tag-lite - Primary Ab Titration GPCR1-Tb 293-GPCR1 (Unlabeled) Labeled Ligand [nm] Tag-lite - Unlabeled Ligand Titration Primary Ab (ug/ml) Positive Control A Positive Control B GPCR1-Tb 0 Unlabeled Ligand [nm] (10 nm Labeled Ligand)

25 Tag-lite GCR1 HTRF 665 ratio CHO/GPCR1 Signal Taglite CHO/GPCR1 Binding of Ab supernatants CHO/GPCR1 - Tag-lite GPCR1-Tb CHO-GPCR1 - FMAT Blue Squares : Positive Controls Red Squares : Taglite Positives GMAP Well CHO-S Signal Many non-specific hits identified when FMAT was used Results much cleaner with Tag-lite 25

26 Ab Binding on Various Cell Lines - FMAT Clone CHO-Ag CHO 293-Ag Ag Snap 293Ag SnapTb 293-irrAg SnapTb Pos.Control MuIgG (-cont) Ab Clone Ab Clone Ab Clone Ab Clone Original screening run in // by FMAT and Tag-lite 26

27 GPCR 2 - HTRF Ligand Binding No ligand binding cell assay available due to low affinity binding of ligand to GPCR receptor No possibility to assess blocking activity of antibodies or small molecule inhibitors Labeled Ligand Titration Unlabeled Ligand Competition (50 nm labeled Ligand) (Negative Control) 50 nm ligand chosen for further inhibition experiments (balancing the needs for maximizing the signal and minimizing ligand concentration) Cold ligand IC50 = ~ 3nM High concentrations of ligand are needed

28 HTRF 665 Ratio Inhibition by Blocking Antibodies 3000 Taglite - GPCR2 - Blocking Antibodies Anti-Ligand Anti-GPCR2 Mouse IgG Antibody (ug/ml) Anti-ligand Ab preincubated with ligand for 15 minutes Anti-GPCR2 and migg preincubated with cells for 15 minutes Both anti-ligand Ab and anti-gpcr2 Ab demonstrate significant inhibition of ligand binding, while irrelevant mouse IgG does not Anti-GPCR2 does not appear to reach 100% inhibition, even when saturating (background is ~500)

29 HTRF GPCR2 (665 ratio) Blocking with Anti-GPCR2 Ligand Supernatants ELISA (binding) versus Tag-lite (blocking) Most blocking supernatants (HTRF Ratio < 2000) have higher Elisa OD signals ELISA GPCR2 Ligand (OD)

30 Taglite Conclusions Strengths: Easy, rapid, robust assay development Faster to execute than FMAT Less influenced by non-specific cell binding Can examine ligand/receptor interactions, even in cases where ligand binding assay could not be established using other means due to low affinity binding of ligand Can use same cells to look at binding and signaling Weaknesses Presence of Snap tag on the receptor can affect antibody binding Labeling ligand can alter its properties

31 Thanks to Matthew Tomlinson Scott Kurose Edwin Haghnazari & Na Tang From Cisbio Paula So Andrea Tatum Anan Chuntharapai Helen Cai Erika Meaddough Peter Brams Keith Joho Nils Lonberg 31

High-Throughput Screening for Antibody Discovery Using Mirrorball

High-Throughput Screening for Antibody Discovery Using Mirrorball High-Throughput Screening for Antibody Discovery Using Mirrorball Christyne Kane Senior Scientist I AbbVie Bioresearch Center Antibody Engineering & Therapeutics December 12, 2017 Christyne Kane is an

More information

Application Note AN001

Application Note AN001 Testing hybridoma supernatants with the Spots On Dots Antibody Screening Kit Application Note AN1 Table of Contents Overview... 2 Figure 1. Screening of hybridomas raised against peptide antigens... 3

More information

Target/drug interference considerations in immunogenicity assessment

Target/drug interference considerations in immunogenicity assessment Target/drug interference considerations in immunogenicity assessment Eric Wakshull, PhD Senior Scientist March 9, 2016 EMA Workshop on Immunogenicity Outline Page 2 Basic Immunogenicity screening assay

More information

Interference from Fc-Fc Interactions in Bridging Immunogenicity Assays for IgG4 mab Therapeutics

Interference from Fc-Fc Interactions in Bridging Immunogenicity Assays for IgG4 mab Therapeutics Interference from Fc-Fc Interactions in Bridging Immunogenicity Assays for IgG4 mab Therapeutics November, 2014 Michael Partridge Ph.D. IgG4 Antibody Therapeutics Natalizumab (Tysabri): Marketed Pembrolizumab

More information

Drug Tolerance in ADA Analysis

Drug Tolerance in ADA Analysis Drug Tolerance in ADA Analysis Matthew Bentley www.eurofins.com DRUG TOLERANCE What is Drug Interference and Why an issue in ADA analysis? Methodologies to overcome Drug Interference Acid Dissociation

More information

Make Your Immunology Research Easy. Kun YIN Associate Director of Marketing Division, GenScript

Make Your Immunology Research Easy. Kun YIN Associate Director of Marketing Division, GenScript Make Your Immunology Research Easy Kun YIN Associate Director of Marketing Division, GenScript GenScript Overview Branch Office Amsterdam, Netherlands Branch Office Tokyo, Japan Headquarter, New Jersey,

More information

Design and Validation of a Non Cell-based Receptor Binding Assay for the Detection of Neutralizing Antibodies to a Biological Therapeutic

Design and Validation of a Non Cell-based Receptor Binding Assay for the Detection of Neutralizing Antibodies to a Biological Therapeutic Design and Validation of a Non Cell-based Receptor Binding Assay for the Detection of Neutralizing Antibodies to a Biological Therapeutic Shalini Gupta Associate Director Amgen, Thousand Oaks, CA shalinig@amgen.com

More information

Immunogenicity Assay Considerations

Immunogenicity Assay Considerations Immunogenicity Assay Considerations Jochem Gokemeijer Jochem.gokemeijer@bms.com Bioassays and Bioanalytics Method Development, Berkley CA October 8 th 2013 Multi Tiered Immunogenicity Assay Approach Screening

More information

Advanced Therapeutic Antibody Discovery with Multiplexed Screening

Advanced Therapeutic Antibody Discovery with Multiplexed Screening Advanced Therapeutic Antibody Discovery with Multiplexed Screening White Paper Scientists need powerful tools that can deliver results to fully understand the ability of candidate antibodies to interrupt

More information

TAG-LITE RECEPTOR LIGAND BINDING ASSAY

TAG-LITE RECEPTOR LIGAND BINDING ASSAY TAG - L I T E TAG-LITE RECEPTOR LIGAND BINDING ASSAY PROTOCOL ASSAY PRINCIPLE The Tag-lite Ligand Binding Assay is a homogeneous alternative to radio ligand binding assays for HTS and compound profiling.

More information

Solutions for Your Research

Solutions for Your Research Solutions for Your Research Custom Antibody Services Polyclonal Monoclonal The service we offer is very complete starting from rabbits, mice and rats. The different formats provided by Primm depend on

More information

Revised Immunogenicity Guideline: Assays and methods- Presentation of the draft guideline and introduction of the topics for discussion

Revised Immunogenicity Guideline: Assays and methods- Presentation of the draft guideline and introduction of the topics for discussion Revised Immunogenicity Guideline: Assays and methods- Presentation of the draft guideline and introduction of the topics for discussion Robin Thorpe & Meenu Wadhwa Revised Guideline: Differences from original

More information

ProteoGenix. Life Sciences Services and Products. From gene to biotherapeutics Target Validation to Lead optimisation

ProteoGenix. Life Sciences Services and Products. From gene to biotherapeutics Target Validation to Lead optimisation ProteoGenix Life Sciences Services and Products From gene to biotherapeutics Target Validation to Lead optimisation ProteoGenix Philippe FUNFROCK, founder and CEO French company located in Strasbourg,

More information

How to run Alpha assay: How to setup an Alpha assay Make your own assay!

How to run Alpha assay: How to setup an Alpha assay Make your own assay! How to run Alpha assay: How to setup an Alpha assay Make your own assay! 1 2009 PerkinElmer AlphaLISA kits - recommendations before starting the assay Samples: Phenol red and hemoglobin: choose AlphaLISA

More information

Biochemical Binding ADCC Assays Utilizing LANCE Toolbox Reagents for the Characterization of higgs and FcγR1A

Biochemical Binding ADCC Assays Utilizing LANCE Toolbox Reagents for the Characterization of higgs and FcγR1A APPLICATION NOTE LANCE TR-FRET Author: Daniel Cardillo PerkinElmer, Inc. Hopkinton, MA Biochemical Binding ADCC Assays Utilizing LANCE Toolbox Reagents for the Characterization of higgs and FcγR1A Introduction

More information

phab Amine and Thiol Reactive Dyes for Antibody Internalization Studies Nidhi Nath, Ph.D. Group Leader, Protein Analysis Promega Corporation

phab Amine and Thiol Reactive Dyes for Antibody Internalization Studies Nidhi Nath, Ph.D. Group Leader, Protein Analysis Promega Corporation phab Amine and Thiol Reactive Dyes for Antibody Internalization Studies Nidhi Nath, Ph.D. Group Leader, Protein Analysis 1 Outline 1. phab Dyes 2. Protocols for conjugating phab Dyes to antibodies 3. Applications:

More information

Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System

Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System ILA Application Note Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System INTRODUCTION Accurate measurement of the concentration

More information

Development of Multiplex Sensitive Anti-Drug Antibody Assays for CRISPR/Cas9 Gene Therapies

Development of Multiplex Sensitive Anti-Drug Antibody Assays for CRISPR/Cas9 Gene Therapies Development of Multiplex Sensitive Anti-Drug Antibody Assays for CRISPR/Cas9 Gene Therapies September 27, 2017 Junxia Wang editasmedicine.com 1 Overview of the presentation Immunogenicity Introduction

More information

Comparing MSD Electrochemiluminescent Detection Technology to Traditional ELISA for Clinical Applications

Comparing MSD Electrochemiluminescent Detection Technology to Traditional ELISA for Clinical Applications Enzyme- linked immunosorbent assays (s) are widely used in clinical settings for detecting substances such as antibodies, peptides, or hormones. However, assays have been gaining in popularity due to advantages

More information

Tag-lite Tachykinin NK1 labeled Cells, ready-to-use (transformed & labeled), 200 tests* (Part# C1TT1NK1)

Tag-lite Tachykinin NK1 labeled Cells, ready-to-use (transformed & labeled), 200 tests* (Part# C1TT1NK1) TAG - L I T E Tag-lite Tachykinin NK1 Receptor Ligand Binding Assay protocol Tachykinin NK1 labeled cells for: 200 tests Part#: C1TT1NK1 Lot#: 03-1 March 2016 (See expiration date on package label) Store

More information

Mouse PECAM-1/CD31 ELISA Kit (mpecam-elisa)

Mouse PECAM-1/CD31 ELISA Kit (mpecam-elisa) Mouse PECAM-1/CD31 ELISA Kit (mpecam-elisa) Cat. No. EK0874 96 Tests in 8 x 12 divisible strips Background Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is an important target GP in DITP. 1 PECAM-1/CD31

More information

MANAGING THE CHALLENGES OF

MANAGING THE CHALLENGES OF 1 MANAGING THE CHALLENGES OF TECHNOLOGY CHANGES IN REGULATED BIOANALYSIS OF BIOTHERAPEUTICS RENUKA C. PILLUTLA, PHD BIOANALYTICAL SCIENCES BRISTOL-MYERS SQUIBB Challenges of Novel / Single-Vendor Technologies

More information

Streamline Your Antibody Enrichment Using Scalable Magnetic Bead-Based Chemistries

Streamline Your Antibody Enrichment Using Scalable Magnetic Bead-Based Chemistries Streamline Your Antibody Enrichment Using Scalable Magnetic Bead-Based Chemistries Samantha Lewis, Ph.D. 2013. Webinar Outline Introduction Magnetic Bead-Based Method Overview Scalability Chemistries o

More information

Chapter 17: Immunization & Immune Testing. 1. Immunization 2. Diagnostic Immunology

Chapter 17: Immunization & Immune Testing. 1. Immunization 2. Diagnostic Immunology Chapter 17: Immunization & Immune Testing 1. Immunization 2. Diagnostic Immunology 1. Immunization Chapter Reading pp. 505-511 What is Immunization? A method of inducing artificial immunity by exposing

More information

1. Immunization. What is Immunization? 12/9/2016. Chapter 17: Immunization & Immune Testing. 1. Immunization 2. Diagnostic Immunology

1. Immunization. What is Immunization? 12/9/2016. Chapter 17: Immunization & Immune Testing. 1. Immunization 2. Diagnostic Immunology Chapter 17: Immunization & Immune Testing 1. Immunization 2. Diagnostic Immunology 1. Immunization Chapter Reading pp. 505-511 What is Immunization? A method of inducing artificial immunity by exposing

More information

Anti-HB-EGF (Human) mab

Anti-HB-EGF (Human) mab Page 1 For Research Use Only. Not for use in diagnostic procedures. CODE No. D308-3 Anti-HB-EGF (Human) mab CLONALITY CLONE ISOTYPE QUANTITY SOURCE IMMUNOGEN FORMURATION STORAGE Monoclonal 3H4 Mouse IgG1

More information

BIOMAN 2015 IMMUNOASSAYS. Barbara Bielska Northampton Community College Tannersville, PA

BIOMAN 2015 IMMUNOASSAYS. Barbara Bielska Northampton Community College Tannersville, PA BIOMAN 2015 IMMUNOASSAYS Barbara Bielska Northampton Community College Tannersville, PA 1 Immunoassays An immunoassay is a biochemical test that measures the presence or concentration of a molecules (typically

More information

SUPPLEMENTARY FIGURES and LEGENDS

SUPPLEMENTARY FIGURES and LEGENDS DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency Nikolas Stefan 1, 3, Patricia Martin-Killias 1, 3, Sascha Wyss-Stoeckle 1,

More information

ELISA. An introduction to the basic principles and assay formats. Innova Biosciences ltd All rights reserved

ELISA. An introduction to the basic principles and assay formats. Innova Biosciences ltd All rights reserved ELISA An introduction to the basic principles and assay formats Agenda Agenda: Introduction to ELISA The different assay types Assay development tips Data analysis tips Troubleshooting tips Indirect vs.

More information

Assays for Immunogenicity: Are We There Yet?

Assays for Immunogenicity: Are We There Yet? Assays for Immunogenicity: Are We There Yet? Mark Wener, MD Department of Laboratory Medicine & Rheumatology Division Department of Medicine University of Washington Seattle, WA 98195 wener@uw.edu Goals:

More information

Human ICAM-2 ELISA Kit

Human ICAM-2 ELISA Kit Human ICAM-2 ELISA Kit CATALOG NO: IRKTAH5128 LOT NO: SAMPLE INTENDED USE For quantitative detection of human ICAM-2 in cell culture supernates, tissue homogenates, serum and plasma (heparin, EDTA). BACKGROUND

More information

INVESTIGATION OF THE BINDING SPECIFICITY OF IGF-IR USING MONOCLONAL ANTIBODIES

INVESTIGATION OF THE BINDING SPECIFICITY OF IGF-IR USING MONOCLONAL ANTIBODIES INVESTIGATION OF THE BINDING SPECIFICITY OF IGF-IR USING MONOCLONAL ANTIBODIES By Mehrnaz Keyhanfar, Pharm.D. A thesis submitted to the University of Adelaide, South Australia in fulfilment of the requirements

More information

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies A Brief Review of Antibody Structure A Brief Review of Antibody Structure The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known

More information

PROF. DR. ASMAA HUSSEIN DIRECTOR OF THE MOLECULAR BIOLOGY RESEARCH UNIT

PROF. DR. ASMAA HUSSEIN DIRECTOR OF THE MOLECULAR BIOLOGY RESEARCH UNIT BY PROF. DR. ASMAA HUSSEIN DIRECTOR OF THE MOLECULAR BIOLOGY RESEARCH UNIT Immunoassay are based on the strong and highly specific interaction occurring between antigens (Ag)) and antibodies (Ab). Ag Ab

More information

Gyrolab ADA assay protocol

Gyrolab ADA assay protocol Gyrolab ADA assay protocol Gyrolab ADA assay protocol D0016561/G, June 2017 Table of Contents Abbreviations... 2 Introduction... 2 Prerequisites... 3 Procedure... 4 Gyrolab ADA protocol overview... 4 Without

More information

The World Leader in SPR Technology. Jimmy Page, PhD, Biacore, Inc.

The World Leader in SPR Technology. Jimmy Page, PhD, Biacore, Inc. The World Leader in SPR Technology Jimmy Page, PhD, Biacore, Inc. Objectives of Biacore Experiments Yes/No Data» Is there binding?» Ligand Fishing Concentration Analysis: How MUCH? Active Concentration

More information

Human ICAM-2 ELISA Kit

Human ICAM-2 ELISA Kit OriGene Technologies, Inc 9620 Medical Center Dr., Suite 200, Rockville, MD 20850 Phone: 1.888.267.4436 Fax: 301-340-9254 Email: techsupport@origene.com Web: Human ICAM-2 ELISA Kit Catalog No. EA100643

More information

RAT/MOUSE NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN (NGAL) ELISA KIT

RAT/MOUSE NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN (NGAL) ELISA KIT RAT/MOUSE NGAL ELISA KIT PAGE 1 RAT/MOUSE NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN (NGAL) ELISA KIT FOR THE QUANTITATIVE DETERMINATION OF NGAL CONCENTRATIONS IN RAT AND MOUSE SERUM AND PLASMA. PURCHASE

More information

Serology as a Diagnostic Technique

Serology as a Diagnostic Technique Serology as a Diagnostic Technique Characteristics of Any Diagnostic Techniques Any useful detection strategy must be: Specific: yield a positive response for only the target organism or molecule. Sensitive:

More information

Mouse CD30L ELISA Kit

Mouse CD30L ELISA Kit Product Information Sheet Catalog No. BO0150 Size 96T Range 15.6pg/ml-1000pg/ml Sensitivity < 1pg/ml Specificity No detectable cross-reactivity with any other cytokine. Storage Store at 4 for frequent

More information

Protein A ELISA for samples containing IgG

Protein A ELISA for samples containing IgG Kit manual of Protein A ELISA for samples containing IgG Catalogue Number: 03-96 Edition: 2006-12-08 IMMUNSYSTEM AB S-751 83 Uppsala, Sweden Phone +46 18 53 89 09 Fax +46 18 53 89 97 www.immunsystem.com

More information

Monoclonal Antibodies

Monoclonal Antibodies Monoclonal Antibodies Homogeneous antibody preparations produced in the laboratory by hybridomas. Produced by identical type of B cell clones. Recognize a signal epitope on an antigen. Hybridoma Technology

More information

Experience the Mabtech ELISA

Experience the Mabtech ELISA Experience the Mabtech ELISA Mabtech provides well-designed ELISA kits for specific, sensitive, and robust quantification of analytes in solution. A comprehensive selection of ELISA kits is available for

More information

6 th EBF Open meeting, Barcelona November 21st, 2013

6 th EBF Open meeting, Barcelona November 21st, 2013 Validation of an immunoassay to selectively quantify the naked antibody of a new Sanofi Antibody Drug Conjugate: an additional tool for improvement of PK interpretation 6 th EBF Open meeting, Barcelona

More information

IgG1 (Human) ELISA Kit

IgG1 (Human) ELISA Kit IgG1 (Human) ELISA Kit Catalog Number KA1730 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General

More information

Mouse IGF-1 ELISA Kit

Mouse IGF-1 ELISA Kit GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Phone: 858.458.0866 Fax: 858.458.0833 Email: sales@genwaybio.com http://www.genwaybio.com Mouse IGF-1 ELISA Kit Catalog No GWB-ZZD063 Size

More information

PD-1 Pathway Recombinant Protein Collection

PD-1 Pathway Recombinant Protein Collection Pathway Recombinant Protein Collection www.acrobiosystems.com Content I. Introduction II. Biotinylated Pathway Proteins IIa. ELISA IIb. FAC Sorting IIc. Biopanning III. HPLC-verified Proteins IV. Full-length

More information

His Tag ELISA Detection Kit

His Tag ELISA Detection Kit His Tag ELISA Detection Kit Technical Manual No. TM0635 Version 12242015 Product Name Cat.No Size His Tag ELISA Detection Kit L00436 1 plate (8 wells x 12 strips) The product is used for rapid and high

More information

ClonePix 2. Screen and select more clones in less time. Genetix Now part of Molecular Devices.

ClonePix 2. Screen and select more clones in less time. Genetix Now part of Molecular Devices. ClonePix 2 Screen and select more clones in less time www.moleculardevices.com/genetix Genetix Now part of Molecular Devices Screen more clones in less time Cut cell line and antibody development times

More information

CD47 / SIRP ALPHA BINDING ASSAY KITS

CD47 / SIRP ALPHA BINDING ASSAY KITS CD47 / SIRP ALPHA BINDING ASSAY KITS PROTOCOL Part # 64ICP02PEG & 64ICP02PEH Test size: 500 tests (64ICP02PEG), 10,000 tests (64ICP02PEH) - assay volume: 20 µl Revision: 02 (July 2017) Store at: -60 C

More information

Biochemical Binding ADCC Assays Utilizing AlphaLISA Toolbox Reagents for the Characterization of higgs and FcγR1A

Biochemical Binding ADCC Assays Utilizing AlphaLISA Toolbox Reagents for the Characterization of higgs and FcγR1A APPLICATION NOTE AlphaLISA Technology Authors: Daniel Cardillo Stephen Hurt PerkinElmer, Inc. Hopkinton, MA Biochemical Binding ADCC Assays Utilizing AlphaLISA Toolbox Reagents for the Characterization

More information

The Trianni Mouse: Best-In-Class Technology for Human Antibody Discovery

The Trianni Mouse: Best-In-Class Technology for Human Antibody Discovery The Trianni Mouse: Best-In-Class Technology for Human Antibody Discovery Corporate Overview Co David Meininger, PhD, MBA Chief Business Officer, Trianni 1 Our Mission Trianni is a biotech company with

More information

Drug Discovery Research Clinical Screening. Comparison of ELISA and AlphaScreen Assay Technologies for Measurement of Protein Expression Levels

Drug Discovery Research Clinical Screening. Comparison of ELISA and AlphaScreen Assay Technologies for Measurement of Protein Expression Levels Drug Discovery Research Clinical Screening FLAG Expression Measurement Application Note Comparison of ELISA and AlphaScreen Assay Technologies for Measurement of Protein Expression Levels ALPHASCREEN APPLICATION

More information

A New Peptide Drug Modality; Helix-Loop-Helix Technology. Interprotein Corporation

A New Peptide Drug Modality; Helix-Loop-Helix Technology. Interprotein Corporation A New Peptide Drug Modality; Helix-Loop-Helix Technology Interprotein Corporation 1 Interprotein Corporation Location: Osaka, Japan Year Established: 2001 CEO & President: Masato Hosoda www.interprotein.com

More information

Human IgG ELISpot BASIC

Human IgG ELISpot BASIC Human IgG ELISpot BASIC Product Code: 3850-2H CONTENTS: Vial 1 (yellow top) Monoclonal antibodies MT91/145 (1.2 ml) Concentration: 0.5 mg/ml Vial 2 (green top) Biotinylated monoclonal antibodies MT78/145

More information

Mouse IgM ELISpot BASIC

Mouse IgM ELISpot BASIC Mouse IgM ELISpot BASIC Product Code: 3885-2H CONTENTS: Vial 1 (green top) Monoclonal antibody MT6A3 (1.2 ml) Concentration: 0.5 mg/ml Vial 2 (yellow top) Biotinylated monoclonal antibody MT9A2 (100 μl)

More information

Human IgG Antigen ELISA Kit

Human IgG Antigen ELISA Kit Human IgG Antigen ELISA Kit Catalog No: IHUIGGKT Lot No: SAMPLE INTENDED USE This human immunoglobulin G antigen assay is intended for the quantitative determination of total human IgG antigen in serum,

More information

ELISA antibody pair Technical Data Sheet

ELISA antibody pair Technical Data Sheet ELISA antibody pair Technical Data Sheet 10-plate and 20-plate format For research use only. Not for use in diagnostic or therapeutic procedures. This Technical Data Sheet applies for the following U-CyTech

More information

Rat sl-selectin ELISA Kit

Rat sl-selectin ELISA Kit Rat sl-selectin ELISA Kit CATALOG NO: IRKTAH5534 LOT NO: SAMPLE INTENDED USE For quantitative detection of rat soluble L-Selectin in cell culture supernates, serum and plasma (heparin, EDTA). BACKGROUND

More information

Human IFN-α (Interferon-Alpha) Pre-Coated ELISA Kit

Human IFN-α (Interferon-Alpha) Pre-Coated ELISA Kit Human IFN-α (Interferon-Alpha) Pre-Coated ELISA Kit Catalog No: 90-2235 1 96 well Format (96 tests) Detection Range: 15.6 1000 pg/ml Sensitivity:

More information

LAMPIRE Hybridoma Project Initiation Form

LAMPIRE Hybridoma Project Initiation Form 1. General Information Company / Institution: Investigator Name: Contact Name: Phone: Fax: 2. Billing / Shipping Information Accounts Payable contact: Company: Dept./Bldg./Room#: Address: Project Name

More information

Human TIMP-2. Pre-Coated ELISA Kit

Human TIMP-2. Pre-Coated ELISA Kit Human TIMP-2 (Metalloproteinase Inhibitor-2) Pre-Coated ELISA Kit Catalog No: 90-2125 1 96 well Format (96 tests) Detection Range: 0.156 10 ng/ml Sensitivity: < 0.094 ng/ml This immunoassay kit allows

More information

Overcoming drug & target interference in ADA and nabassays

Overcoming drug & target interference in ADA and nabassays Overcoming drug & target interference in ADA and nabassays Robert Nelson Ph.D. MRQA Head of Bioanalytical Laboratory, Novimmune SA 1 Presentation Outline Case Study 1 Drug & target interference in a clinical

More information

Recruitment of Grb2 to surface IgG and IgE provides antigen receptor-intrinsic costimulation to class-switched B cells

Recruitment of Grb2 to surface IgG and IgE provides antigen receptor-intrinsic costimulation to class-switched B cells SUPPLEMENTARY FIGURES Recruitment of Grb2 to surface IgG and IgE provides antigen receptor-intrinsic costimulation to class-switched B cells Niklas Engels, Lars Morten König, Christina Heemann, Johannes

More information

FIVEphoton Biochemicals

FIVEphoton Biochemicals Human Insulin Degrading Enzyme (IDE) ELISA Kit Biotin Detection Antibody Format 96T FIVEphoton Biochemicals. For research use only. Not for diagnostics. Part No. hide-biotin(96t) FIVEphoton Biochemicals

More information

Human IgG ELISA Kit. Strip well format. Reagents for up to 96 tests

Human IgG ELISA Kit. Strip well format. Reagents for up to 96 tests Human IgG ELISA Kit Strip well format. Reagents for up to 96 tests Catalog No. CS222A Quantity: 1 x 96 tests CS222B 5 x 96 tests Intended Use: Background: Assay Principle: This human immunoglobulin G antigen

More information

Mouse TNF alpha ELISA Kit

Mouse TNF alpha ELISA Kit Mouse TNF alpha ELISA Kit Catalog No. GWB-ZZD049 Size 96 wells/kit Sandwich ELISA kit for quantitative detection of mouse TNF alpha in cell culture supernates, serum and plasma(heparin, EDTA). Typical

More information

Highly Sensitive Detection of the Interaction Occurring Between Phage Displayed Peptides and their Target using AlphaScreen

Highly Sensitive Detection of the Interaction Occurring Between Phage Displayed Peptides and their Target using AlphaScreen Highly Sensitive Detection of the Interaction Occurring Between Phage Displayed Peptides and their Target using AlphaScreen N. Rouleau, P. Allard, P. Roby, D. Sexton*, F. Whelihan* and R. Bossé * DYAX

More information

For quantitative detection of mouse IGF-1 in serum, body fluids, tissue lysates or cell culture supernatants.

For quantitative detection of mouse IGF-1 in serum, body fluids, tissue lysates or cell culture supernatants. Mouse IGF-1 ELISA Kit (migf-1-elisa) Cat. No. EK0378 96 Tests in 8 x 12 divisible strips Background Insulin-like growth factor 1 (IGF-1), also known as somatomedin C, is a polypeptide protein hormone similar

More information

Mouse TNF alpha ELISA Kit

Mouse TNF alpha ELISA Kit Mouse TNF alpha ELISA Kit Catalog No. GWB-ZZD049 Size 96 wells/kit Sandwich ELISA kit for quantitative detection of mouse TNF alpha in cell culture supernates, serum and plasma(heparin, EDTA). Typical

More information

Mouse thyroxine(t4) ELISA Kit

Mouse thyroxine(t4) ELISA Kit Mouse thyroxine(t4) ELISA Kit Catalog Number. CSB-E05083m For the quantitative determination of endogenic mouse thyroxine(t4) concentrations in serum, plasma, tissue homogenates. This package insert must

More information

ACE2 (Human) ELISA Kit

ACE2 (Human) ELISA Kit ACE2 (Human) ELISA Kit Catalog Number KA4223 96 assays Version: 10 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...

More information

Rat thyroxine(t4) ELISA Kit

Rat thyroxine(t4) ELISA Kit Rat thyroxine(t4) ELISA Kit Catalog Number. CSB-E05082r For the quantitative determination of endogenic rat thyroxine (T4) concentrations in serum, plasma, cell culture supernates, tissue homogenates,

More information

PicoKine ELISA. Catalog number: EK1326

PicoKine ELISA. Catalog number: EK1326 PicoKine ELISA Catalog number: EK1326 For the quantitation of Human IGF2R concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This package insert must be read in

More information

Data Sheet CD137/NF-κB Reporter - HEK293 Recombinant Cell Line Catalog # 79289

Data Sheet CD137/NF-κB Reporter - HEK293 Recombinant Cell Line Catalog # 79289 Data Sheet CD137/NF-κB Reporter - HEK293 Recombinant Cell Line Catalog # 79289 Background Human CD137 (4-1BB; TNFRS9) is an inducible co-stimulatory molecule that activates T cells. CD137:CD137L-mediated

More information

Deer insulin-like growth factors 1 (IGF-1) ELISA Kit. This package insert must be read in its entirety before using this product.

Deer insulin-like growth factors 1 (IGF-1) ELISA Kit. This package insert must be read in its entirety before using this product. Deer insulin-like growth factors 1 (IGF-1) ELISA Kit Catalog Number. For the quantitative determination of deer insulin-like growth factors 1 (IGF-1) concentrations in serum, plasma, tissue homogenates.

More information

MSD GOLD Streptavidin and Avidin Plates

MSD GOLD Streptavidin and Avidin Plates MSD GOLD Streptavidin and Avidin Plates SECTOR Plates QUICKPLEX Plates 96-well Streptavidin L15SA L55SA 96-well Small Spot Streptavidin L45SA N/A 96-well High Bind Avidin L15AB L55AB FOR RESEARCH USE ONLY.

More information

Custom Antibody Services. Antibodies Designed Just for You. HuCAL Recombinant Monoclonal Antibody Generation Service

Custom Antibody Services. Antibodies Designed Just for You. HuCAL Recombinant Monoclonal Antibody Generation Service Custom Antibody Services Antibodies Designed Just for You HuCAL Recombinant Monoclonal Antibody Generation Service Antibodies Designed Just for You What if there was a technology that would allow you to

More information

Rat IGF-1 ELISA Kit (rigf-1-elisa)

Rat IGF-1 ELISA Kit (rigf-1-elisa) Rat IGF-1 ELISA Kit (rigf-1-elisa) Cat. No. EK0377 96 Tests in 8 x 12 divisible strips Background Insulin-like growth factor 1 (IGF-1), also known as somatomedin C, is a polypeptide protein hormone similar

More information

IMPACT OF HIGHER ORDER COMPLEXES ON BIOMARKER TARGET QUANTITATION

IMPACT OF HIGHER ORDER COMPLEXES ON BIOMARKER TARGET QUANTITATION IMPACT OF HIGHER ORDER COMPLEXES ON BIOMARKER TARGET QUANTITATION SURENDRAN RAJENDRAN Bristol-Myers Squibb Immunogenicity and Bioassay Summit 2014 - Immunogenicity Assessment & Clinical Relevance - Assay

More information

FIVEphoton Biochemicals

FIVEphoton Biochemicals Apo E ELISA Kit Biotin Detection Antibody Format 96T. General Protocol. FIVEphoton Biochemicals For research use only. Not for diagnostics. Part No. mapoe-biotin Use this protocol as a general guide. For

More information

Androgen Receptor (Phospho-Tyr363) Colorimetric Cell-Based ELISA Kit. Catalog #: OKAG02138

Androgen Receptor (Phospho-Tyr363) Colorimetric Cell-Based ELISA Kit. Catalog #: OKAG02138 Androgen Receptor (Phospho-Tyr363) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02138 Please read the provided manual entirely prior to use as suggested experimental protocols may have changed. Research

More information

Rat Testosterone (T) ELISA Kit (OKCA00179)

Rat Testosterone (T) ELISA Kit (OKCA00179) Rat Testosterone (T) ELISA Kit (OKCA00179) For the quantitativ e determination of endogenic rat testosterone concentrations in serum, plasma, cell culture supernatants, tissue homogenates. This package

More information

PicoKine ELISA. Catalog number: EK0544

PicoKine ELISA. Catalog number: EK0544 PicoKine ELISA Catalog number: EK0544 For the quantitation of Human KDR concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This package insert must be read in its

More information

Hitting the target in phenotypic drug discovery: Advances in receptor deconvolution

Hitting the target in phenotypic drug discovery: Advances in receptor deconvolution Hitting the target in phenotypic drug discovery: Advances in receptor deconvolution Jim Freeth, Retrogenix, UK Jim.Freeth@retrogenix.com ELRIG Research and Innovation Meeting March 11th 2014 The right

More information

FDA Draft Guidance on Immunogenicity Testing

FDA Draft Guidance on Immunogenicity Testing FDA Draft Guidance on Immunogenicity Testing Susan Kirshner, Ph.D. Associate Chief, Laboratory of Immunology Division of Therapeutic Proteins OBP/CDER/FDA EBF 2010 Guidance for Industry Assay Development

More information

Exceptional Human Antibody Discovery. Corporate Overview

Exceptional Human Antibody Discovery. Corporate Overview Exceptional Human Antibody Discovery Corporate Overview Co 1 1 Our Mission Trianni is a biotech company with the scientific mission of creating optimized and highly versatile platforms for generation of

More information

Perform reproducible immunoprecipitation in less than 40 minutes

Perform reproducible immunoprecipitation in less than 40 minutes Perform reproducible immunoprecipitation in less than 40 minutes Dynabeads products Immunoprecipitation made easy with low nonspecific binding, high yield, and reproducibility Magnetic beads have become

More information

Discovery and Humanization of Novel High Affinity Neutralizing Monoclonal Antibodies to Human IL-17A

Discovery and Humanization of Novel High Affinity Neutralizing Monoclonal Antibodies to Human IL-17A Discovery and Humanization of Novel High Affinity Neutralizing Monoclonal Antibodies to Human IL-17A Contacts: Marty Simonetti martysimonetti@gmail.com Kirby Alton kirby.alton@abeomecorp.com Rick Shimkets

More information

Chicken Calprotectin ELISA Kit. User Manual

Chicken Calprotectin ELISA Kit. User Manual Nordic BioSite AB Propellervägen 4A, 183 62 Täby, Sweden T +46 (0)8 544 433 40, F +46 (0)8 756 94 90 info@nordicbiosite.com, www.nordicbiosite.com Org. No: 556539-9374, Residence: Täby Chicken Calprotectin

More information

Antibody Generation: challenges and solutions. Glen Marszalowicz, PHD May 10, AM

Antibody Generation: challenges and solutions. Glen Marszalowicz, PHD May 10, AM Antibody Generation: challenges and solutions Glen Marszalowicz, PHD May 10, 2015 9AM GenScript the most cited biology CRO CRISPR Services Gene Services Peptide Services Protein Services Antibody Services

More information

RAT MONOCLONAL ANTIBODIES ANTI-MOUSE IMMUNOGLOBULINS

RAT MONOCLONAL ANTIBODIES ANTI-MOUSE IMMUNOGLOBULINS RAT MONOCLONAL ANTIBODIES ANTI-MOUSE IMMUNOGLOBULINS Supplied by: dianova GmbH Warburgstrasse 45 20354 Hamburg Phone: +49 (0)40 45 06 70 Email: info@dianova.de www.dianova.com MOUSE IMMUNOGLOBULINS 1 (Sub)classes

More information

Human IgG ELISpot BASIC

Human IgG ELISpot BASIC Distribuito in ITALIA da Li StarFish S.r.l. Via Cavour, 35 20063 Cernusco S/N (MI) telefono 02-92150794 fax 02-92157285 info@listarfish.it www.listarfish.it Human IgG ELISpot BASIC Product Code: 3850-2A

More information

MyBioSource.com. Human VEGF ELISA Kit

MyBioSource.com. Human VEGF ELISA Kit Human VEGF ELISA Kit Catalog No.: MBS355343 Size: 96T Range: 31.2 pg/ml-2000 pg/ml (human serum, plasma, body fluids) 15.6 pg/ml-1000 pg/ml (cell culture supernates) Sensitivity < 1 pg/ml Storage and Expiration:

More information

PicoKine ELISA. Catalog number: EK1316

PicoKine ELISA. Catalog number: EK1316 PicoKine ELISA Catalog number: EK1316 For the quantitation of Human CRP concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This package insert must be read in its

More information

Anti-Tilapia (Oreochromis niloticus) IgM monoclonal antibody. Product no: F04

Anti-Tilapia (Oreochromis niloticus) IgM monoclonal antibody. Product no: F04 Anti-Tilapia (Oreochromis niloticus) IgM monoclonal antibody Product no: F04 Product Description This monoclonal antibody (Mab) reacts with Anti-Tilapia (Oreochromis niloticus) immunoglobulin M (IgM).

More information

Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction

Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction APPLICATION NOTE AlphaLISA Technology Authors: Hong Cao Jen Carlstrom PerkinElmer, Inc. Hopkinton, MA Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction Introduction

More information

Mouse Betacellulin/BTC ELISA Kit

Mouse Betacellulin/BTC ELISA Kit OriGene Technologies, Inc 9620 Medical Center Dr., Suite 200, Rockville, MD 20850 Phone: 1.888.267.4436 Fax: 301-340-9254 Email: techsupport@origene.com Web: Mouse Betacellulin/BTC ELISA Kit Catalog No.

More information

MyBioSource.com. Human Vitamin K2 ELISA USER INSTRUCTION

MyBioSource.com. Human Vitamin K2 ELISA USER INSTRUCTION Human Vitamin K2 ELISA USER INSTRUCTION Cat.No MBS163021 Standard Curve Range: 5ng/ml - 1000ng/ml Sensitivity: 2.52ng/ml Size: 96 wells Storage: Store the reagents at 2-8 C. For over 6-month storage refer

More information

Rat Neurotrophin-3 ELISA Kit

Rat Neurotrophin-3 ELISA Kit Rat Neurotrophin-3 ELISA Kit GenWay ID: GWB-ZZD026 Size 96T(8 12 divisible strips) For quantitative detection of rat Neurotrophin-3 in cell culture supernates and serum. Typical Data Obtained from Rat

More information