Recombinant Antibody Production in Therapeutic Antibody Projects. Keshav Vasanthavada Senior Marketing Specialist, GenScript April 7, 2016
|
|
- Walter Ferguson
- 6 years ago
- Views:
Transcription
1 Recombinant Antibody Production in Therapeutic Antibody Projects Keshav Vasanthavada Senior Marketing Specialist, GenScript April 7, 2016
2 Presentation Outline Introduction Recombinant Ab Production GenScript Capabilities & Services Case Studies Summary & Conclusion Make Research Easy 2
3 Introduction Make Research Easy 3
4 About GenScript Gene Cell Line Peptide Discovery Biology GenScript Products & Services Make Research Easy Protein Antibody Make Research Easy Introduction 4
5 Expression Systems Introduction Make Research Easy 5
6 Production Track Record Track Record >6000 batches of high quality protein >1500 batches of high quality rabs >92% success rate for all projects Successfully delivered a variety of protein targets We deliver guaranteed and fully customizable, high quality recombinant antibody and protein services with fast turnaround timelines and at very competitive prices Introduction Make Research Easy 6
7 GenScript Ab Drug Discovery Services Introduction Make Research Easy 7
8 Recombinant Ab (rab) Production Make Research Easy 8
9 Why rabs? Traditional mab technology first developed in 1975 by Kӧhler and Milstein. Mature technology, very robust and cost-effective. Issues include Ethical concerns Non-specificity Instability of hybridomas causing loss of Ab genes Potential immunogenicity (HAMA response) Irreproducibility Series of Nature articles reported on the reproducibility crisis in biomedical sciences caused by Abs. Nearly $350M/year wasted on irreproducible Abs. Scientists at leading organizations including >100 co-signatories proposed shifting entirely towards rabs. Recombinant Ab Production Make Research Easy 9
10 Advantages of rabs Non-animal technology More control over the process Speed production time in weeks HTP processing Can be constructed against virtually any antigen Immunogenicity not an issue rabs can be produced in several formats: Fabs, scfv, diabodies Amenable to fusion with drugs and toxins Sequence-defined making them more reliable and reproducible Conducive to optimization and engineering There are various ways in which a researcher can utilize rab technology rab technology is not intended to entirely replace conventional technologies. In most cases rab technology supplements existing technologies. Recombinant Ab Production Make Research Easy 10
11 Entry Point into rab Production Display Transgenic Animal Hybridoma rab Production Other Recombinant Ab Production Make Research Easy 11
12 GenScript Capabilities & Services Make Research Easy 12
13 Options for rab Production Transient Expression 50ug to gram-level expression Rapid - as early as 6 weeks Starting from $499 Production Technologies Stable Cell Pool 300mg to gram-level expression As early as 8 weeks Competitive pricing Stable Clonal Cell Line Multiple gram-level expression Between weeks Suitable for large scale consistent production of your rab GenScript Capabilities & Services Make Research Easy 13
14 Transient Expression Licensed NRC Technologies Licensed from NRC, Canada HEK293 Highly stable 293EBNA1 clone. Afford the advantages of serum-free production. Rapid and high yield recombinant production. CHO-pTT Provides comparable protein expression level to those of HEK293 system. Rapid production of recombinant proteins that require post-translation modification for research or pharmaceutical use. GenScript Capabilities & Services Make Research Easy 14
15 Stable Cell Pool Use of GenScript Technology results in high-producing cell pools. Cell pools achieved within 8 weeks GenScript Capabilities & Services Make Research Easy 15
16 Titer (g/l) GenScript CHO-GS Stable Cell Line GS GS based selection system High productivities without amplification Speed no need for rounds of gene amplification Scalable CHO CHO derived host cell line for expression Adapted to suspension and AFCDM Selected for superior growth and expression Cell Line Development Fully optimized process Product concentrations of >5g/L achieved High probability of monoclonality 6 4 mab Expression level High volumetric productivity 2 0 Pool Single cell clone Batch culture Fed-batch round 1 Fed-batch round 2 Cost mab Expression level GenScript has created cell lines producing >5g/L of recombinant antibody with production rates in the range pg/cell/day. GenScript Capabilities & Services Make Research Easy 16
17 General Schematic of rab Production GenScript Capabilities & Services Make Research Easy 17
18 Industry Pipeline Discovery Preclinical Development GLP Workup GMP Workup Target Validation Screening Preparation Lead Generation Lead Optimization & Characterization Lead Selection No. Examples 1 HTP expression and purification of recombinant proteins & antibodies: Quantity: 1-5ml, purification: 96 well plates Protein A column 2 Lab scale transient expression in shaking flasks (100ml-10L): Transient expression in mammalian cells, mostly HEK293 3 Mid scale expression in wave bags (10L-25L): Transient (HEK293) or Stable pool production (CHO cells) 4 5 Large scale wave bags or bioreactor (varies): Transient or Stable pool production (CHO cells, >25L), or Stable cell line production (CHO-GS, >10L, deliver cell line) Characterization services with LC-MS as the core technology: Critical for verification and the release of bio products, more important in the latter stage of R&D, absolutely required in clinical stage GenScript Capabilities & Services Make Research Easy 18
19 rab Service Guide With solid expertise in recombinant antibody (rab) production techniques, GenScript provides a comprehensive rab service portfolio. Choose from services that deliver microgram to gram quantities of pure rab for each stage of your Ab drug discovery program Purpose Screening Candidate validation In vitro efficacy In vivo efficacy Safety test with GLP *Not including gene synthesis # Including gene synthesis Service HTP Gene to Antibody Production MamPower guaranteed recombinant antibody production MamPower guaranteed recombinant antibody production Large scale recombinant antibody production Large scale recombinant antibody production Custom Services Custom Services Production Type HTP Transient Expression Transient Expression in Shaking flask Transient Expression in Shaking flask Transient Expression in Wavebag Volume Wavebag cell 10L-25L pool Bioreactor cell 25L-100L pool Gram Level Bioreactor cell Bioproduction cell line 10L-100L line service Antibody quantity Timeline Guiding Price per unit QC standard 1ml-5ml 40μg-200μg 2-3 weeks ~$300 per variant* Titer: ELISA Purity: SDS-PAGE -85% Quantitation: UV A280 Titer: ELISA 0.1L-1L 4mg-100mg 3-4 weeks $30-$500/mg antibody # Purity: SDS-PAGE -90% Quantitation: UV A280 1L-10L 40mg-1g 4-5 weeks $12-75/mg antibody # 10L-25L 400mg-2.5g 6-8 weeks $10-30/mg antibody # 1g-12.5g 2.5g-50g 10g-100g weeks weeks Titer: ELISA Purity: SDS-PAGE -95%; HPLC -95% Quantitation: UV A280 Endotoxin:<1EU/mg Titer: ELISA Purity: SDS-PAGE -95%/99%; HPLC -95%/99% Quantitation: UV A280 $3.2-22/mg antibody # Endotoxin:<1EU/mg or <0.1EU/mg $2.4-16/mg antibody # Titer: ELISA Purity: SDS-PAGE -95%/99%; HPLC -95%/99% Quantitation: UV A280 $1.5-6/mg antibody # Endotoxin:<1EU/mg or <0.1EU/mg Identification: MS Make Research Easy weeks GenScript Capabilities & Services
20 MamPower Guaranteed Services Customer provides Amount Purity & endotoxin level options Timeline Deliverables Price starting from Antibody sequence 5mg 95% by SDS-PAGE 5-6 weeks 10mg 25mg 50mg 100mg 250mg 500mg 95% by SDS-PAGE Endotoxin 10EU/mg Concentration 1mg/ml 95% by HPLC Endotoxin 1EU/mg 6-8 weeks 8-10 weeks LC and HC in puc57 vector Optimized gene sequence report Purified antibody at listed amount and purity QC data $2,999 Key features of MamPower guaranteed recombinant antibody production services Gene synthesis is included in the package no additional cost to you. Guarantee recombinant antibody amount, purity and endotoxin control no cost to you if we don't deliver as promised. Flexible scale from 5mg to 500mg. Competitive price. Fast turnaround time - as little as 5 weeks. Make Research Easy GenScript Capabilities & Services 20
21 HTP Gene to Antibody Service Workflow Ideal for Antibody Screening and Optimization Campaigns Minimum 24 Abs required 50ug or 1mg packages 6-8 weeks Starting at $499/Ab (including gene synthesis) GenScript Capabilities & Services Make Research Easy 21
22 Case Studies Make Research Easy 22
23 Case Study 1: Low Expression Expression level was low (2.5 mg/l) Optimized Antibody Sequence OptimumGene TM Codon Optimization Optimized Transfection and Purification Expression level was significantly increased (25 mg/l) Make Research Easy Case Studies 23
24 Case Study 2: No Expression Target: Antibody 1 (MW ~ 150 kda) Western blot Western blot Fig.1. SDS-PAGE and Western blot analysis of Antibody 1 Lane M: Marker Lane 1~3: Cell culture supernatant from day 2, 4 and 6 post-transfection R (293) Lane 4~6: Cell culture supernatant from day 2, 4 and 6 post-transfection R(CHO) Lane 7~9: Cell culture supernatant from day 2, 4 and 6 post-transfection N (293) Lane 10~12: Cell culture supernatant from day 2, 4 and 6 post-transfection N (CHO) Lane P: Mouse IgG1, Kappa (Sigma, Cat.No.M9269) as positive control The target Antibody has no expression (left panel) Upon sequence revision, the expression level of target antibody reached ~15 mg/l. Case Studies Make Research Easy 24
25 Case Study 3: Client HTP Ab Production Client has access to a proprietary technology for identifying and isolating antigen-specific B-cells. Using a group of patients [4, healthy immunocompetent] who contracted and cleared HIV infection: Identified B-cells specific for the desired epitope Isolated and sequenced the unique antibody sequences Cloned, purified and confirmed reactivity of antibodies Engaged GenScript to continue pre-clinical development of a panel of positive Abs. Client focused on biology and developability of lead Abs to ensure efficacy while minimizing downstream risks. Case Studies Make Research Easy 25
26 Case Study 3 Specifics Screening & Candidate Validation Biotech Client 4, patient-derived anti-hiv neutralizing Abs Customer in-silico optimized sequences for production and development of Abs HTP GtA, 1mg HTP Biophysical Characterization [thermal stability, aggregation propensity] MamPower TM and customized rab services Few 100 mgs for: Viscosity screening Colloidal stability Solubility Stability Screening assay Filterability Finally 3-10 Abs Identify that are active and with desirable biophysical properties Case Studies Make Research Easy 26
27 Case Study 3 Specifics In vitro & In vivo efficacy & Safety Few 100mgs required for: MamPower TM, Large scale rab In vitro neutralization assays [current strategies to measure the neutralization activity of anti-hiv-1 mabs involve wellestablished TZM-bl cell-based assays] Few grams required for: Large scale rab, Custom services cell pool In vivo protective activity of anti-hiv-1 Ab in mouse and macaques Safety test Cell pool, Cell line Case Studies Make Research Easy 27
28 Summary & Conclusion Make Research Easy 28
29 GenScript Capability >1500 batches of high quality rabs BiScFv C-Fc tag protein N-Fc tag protein VL V H ScFv1 Linker ScFv2 V H VL His Summary & Conclusion Make Research Easy 29
30 Quotations & Ordering Workflow Summary & Conclusion Make Research Easy 30
31 Why Choose GenScript Summary & Conclusion Make Research Easy 31
32 Antibody Handbook Topics include Antibody structure, isotypes and formats Antibody fragments and advantages Antibody drug discovery process Humanization and Affinity maturation rab Production rab purification and characterization and more Summary & Conclusion Make Research Easy 32
33 Upcoming Webinars To register for other webinars in the GenScript Webinar Series visit April 12, 2016 / 2:00 PM EST Genetic variants: tips & techniques for creating novel synthetic genes Laura Geuss, Ph.D. April 21, 2016 / 9:00 AM EST Anti-idiotype antibody generation and application in antibody drug discovery - Liusong Yin, Ph.D. April 28, 2016/ 9:00 AM EST CRISPR grna libraries: design options, applications, and how they can accelerate your genetic screening studies Peter Dai, Ph.D. May 10, 2016 / 9:00 AM EST Antibody generation: challenges and solutions Glen Marszalowicz, Ph.D. May 18, 2016/ 9:00 AM EST Recombinant protein production in baculovirus/insect expression system Yong Liu, Ph.D. keshav.vasanthavada@genscript.com Summary & Conclusion Make Research Easy 33
34 Thank You! Make Research Easy 34
Antibody Services from GenScript
Services from GenScript www.genscript.com GenScript USA Inc. 860 Centennial Ave., Piscataway, NJ 08854 USA Toll-Free: 1-877-436-7274 Fax: 1-732-210-0262 1-732-855-5878 Academic Services Pharmaceutical
More informationCustom Antibody Services. Antibodies Designed Just for You. HuCAL Recombinant Monoclonal Antibody Generation Service
Custom Antibody Services Antibodies Designed Just for You HuCAL Recombinant Monoclonal Antibody Generation Service Antibodies Designed Just for You What if there was a technology that would allow you to
More informationProteoGenix. Life Sciences Services and Products. From gene to biotherapeutics Target Validation to Lead optimisation
ProteoGenix Life Sciences Services and Products From gene to biotherapeutics Target Validation to Lead optimisation ProteoGenix Philippe FUNFROCK, founder and CEO French company located in Strasbourg,
More informationChaperone Co-expression Strategies for Soluble Protein Production in E. coli
Chaperone Co-expression Strategies for Soluble Protein Production in E. coli Bo Wu, Ph.D. Bo.Wu@genscript.com Table of Contents 1 2 3 4 5 6 E. Coli Expression Overview Inclusion Bodies & Refolding Expression
More informationAntibody Generation: challenges and solutions. Glen Marszalowicz, PHD May 10, AM
Antibody Generation: challenges and solutions Glen Marszalowicz, PHD May 10, 2015 9AM GenScript the most cited biology CRO CRISPR Services Gene Services Peptide Services Protein Services Antibody Services
More informationGenes to Proteins to Antibodies
Genes to Proteins to Antibodies About Us Fusion Antibodies is a CRO established in 2001 as a spin-out from Queen s University Belfast. The company building is situated in a charming area of Springbank
More informationExpression of Next Generation Biologics Requires Next Generation Expression Systems
Expression of Next Generation Biologics Requires Next Generation Expression Systems Joachim Klein, Ph.D., Associate Director, Head of Strain Development and Cell Banking Georg Blaser, Ph.D., Associate
More informationWestern-GUARANTEED Antibody Service FAQ
Western-GUARANTEED Antibody Service FAQ Content Q 1: When do I need a Western GUARANTEED Peptide Antibody Package?...2 Q 2: Can GenScript provide a Western blot guaranteed antibody?...2 Q 3: Does GenScript
More informationCHO-GSN PLATFORM STABLE CELL LINE GENERATION. NR v5
CHO-GSN PLATFORM STABLE CELL LINE GENERATION NR3160 20180122 v5 Highlights of LakePharma s CHO-GSN Cell Line Platform 2 LakePharma proprietary technology Complete cell line lineage and clear path to commercialization,
More informationACCELERATE YOUR ANTIBODY DRUG DISCOVERY & DEVELOPMENT
ACCELERATE YOUR ANTIBODY DRUG DISCOVERY & DEVELOPMENT Since 2004, GenScript has been customizing antibodies for widespread applications. Our team has experience with over 150 antibody drug discovery projects.
More informationFusion Antibodies. Genes to Proteins to Antibodies
Fusion Antibodies Genes to Proteins to Antibodies Why Fusion Antibodies? 12 years experience of Antibody drug development Milestoned Payments European based limited company High Quality outcomes for the
More informationPartnered Discovery of High-Quality Antibody Drug Candidates. Company Presentation
Partnered Discovery of High-Quality Antibody Drug Candidates Company Presentation AbCheck s Unique Offering A unique source of human antibodies with one of the industry's most versatile technology platforms
More informationPeptide libraries: applications, design options and considerations. Laura Geuss, PhD May 5, 2015, 2:00-3:00 pm EST
Peptide libraries: applications, design options and considerations Laura Geuss, PhD May 5, 2015, 2:00-3:00 pm EST Overview 1 2 3 4 5 Introduction Peptide library basics Peptide library design considerations
More informationSynthetic Biology. Sustainable Energy. Therapeutics Industrial Enzymes. Agriculture. Accelerating Discoveries, Expanding Possibilities. Design.
Synthetic Biology Accelerating Discoveries, Expanding Possibilities Sustainable Energy Therapeutics Industrial Enzymes Agriculture Design Build Generate Solutions to Advance Synthetic Biology Research
More informationPosition1: Senior Scientist/Principal Scientist
Position1: Senior Scientist/Principal Scientist Field: Immunology, Immuno-Oncology Department: Discovery Biology : o Develop and conduct cell or immune based assays to support drug discovery in cancer
More informationUpstream mammalian cell processing challenges and prospects
BioProcess International Berlin, April 11-14, 2005 Upstream mammalian cell processing challenges and prospects John Birch Lonza Significance of Mammalian Cell Processes Commercial significance of biopharmaceutical
More informationRECENT IMPROVEMENTS TO LONZA S GLUTAMINE SYNTHETASE (GS) GENE EXPRESSION SYSTEM. Dr Robert Gay
RECENT IMPROVEMENTS TO LONZA S GLUTAMINE SYNTHETASE (GS) GENE EXPRESSION SYSTEM Dr Robert Gay Lonza Biologics plc, 2004 The Challenge of the MAb Market Global market for Monoclonal Antibody Therapeutics
More informationBioreactor Scale-Up of BacMam-Transduced CHO-S Cells for the Production of Soluble Recombinant Proteins
Bioreactor Scale-Up of BacMam-Transduced CHO-S Cells for the Production of Soluble Recombinant Proteins Chris Kemp and April Birch Kempbio, Inc. Frederick, MD USA Presentation Summary BacMam Overview BacMam
More informationConverting rabbit hybridoma into recombinant antibodies with effective transient production in an optimized human expression system
Converting rabbit hybridoma into recombinant antibodies with effective transient production in an optimized human expression system Dr. Tim Welsink Molecular Biology Transient Gene Expression OUTLINE Short
More informationPartnered Discovery of High-quality Antibody Drug Candidates COMPANY PRESENTATION DECEMBER 2016
Partnered Discovery of High-quality Antibody Drug Candidates COMPANY PRESENTATION DECEMBER 2016 Corporate Overview A unique source of therapeutic human antibodies with one of the industry's most versatile
More informationOptimization Strategies of Expression Cell Line Construction to Reduce the Biological Drug Development Risk. Feng Gao, MD, PhD. AutekBio CO.
Optimization Strategies of Expression Cell Line Construction to Reduce the Biological Drug Development Risk Feng Gao, MD, PhD AutekBio CO. Expression Systems used for Approved Antibody and Antibody-Related
More informationStreamline Your Antibody Enrichment Using Scalable Magnetic Bead-Based Chemistries
Streamline Your Antibody Enrichment Using Scalable Magnetic Bead-Based Chemistries Samantha Lewis, Ph.D. 2013. Webinar Outline Introduction Magnetic Bead-Based Method Overview Scalability Chemistries o
More informationFuture Perspectives of Antibody Manufacturing
BioProduction 2005 Amsterdam Future Perspectives of Antibody Manufacturing John Birch Lonza Biologics Monoclonal Antibodies A Success Story Fastest growing segment of the pharmaceutical market Sales forecast
More informationPartnered Discovery of High-quality Antibody Drug Candidates
Partnered Discovery of High-quality Antibody Drug Candidates COMPANY PRESENTATION SEPTEMBER 2017 Corporate Overview A unique source of therapeutic human antibodies with one of the industry's most versatile
More informationRapid selection of high yielding GS-CHO cell lines using the GS expression system in a protein-free, chemically defined, animal component-free process
Rapid selection of high yielding GS-CHO cell lines using the GS expression system in a protein-free, chemically defined, animal component-free process David Mainwaring, Lonza Biologics. Introduction Chemically
More informationSolutions for Your Research
Solutions for Your Research Custom Antibody Services Polyclonal Monoclonal The service we offer is very complete starting from rabbits, mice and rats. The different formats provided by Primm depend on
More informationMaximizing Assembly and Yield of Unmodified Bispecific Antibodies
Maximizing Assembly and Yield of Unmodified Bispecific Antibodies Pauline Malinge Head of Molecular Interaction Facility 1 Outline Introduction The κλ body format The κλ body platform Bispecific Antibody
More informationMammalian Expression Platform
Mammalian Expression Platform Partners for Life Advancing tomorrow s medicines w Your CDMO partner for biologics and advanced therapies Good science, experience and a quality driven approach Line/Strain
More informationCellca Technology Platform
Cellca Technology Platform Custom Cell Line & Process Development April 2018 Overview: Cellca Technology Platform Cellca CMO/Client Cell Line Development Process Development Process Transfer GMP Production
More informationAPPROACHES TO IMPROVING THE PERFORMANCE OF MAMMALIAN CELL CULTURES FOR PROTEIN PRODUCTION
BioLOGIC USA BOSTON, 20 th OCTOBER 2004 APPROACHES TO IMPROVING THE PERFORMANCE OF MAMMALIAN CELL CULTURES FOR PROTEIN PRODUCTION Dr Robert Gay Lonza Biologics 2004 The Challenge of the MAb Market Global
More informationDiscovery and Humanization of Novel High Affinity Neutralizing Monoclonal Antibodies to Human IL-17A
Discovery and Humanization of Novel High Affinity Neutralizing Monoclonal Antibodies to Human IL-17A Contacts: Marty Simonetti martysimonetti@gmail.com Kirby Alton kirby.alton@abeomecorp.com Rick Shimkets
More informationIntroduction of Development Center for Biotechnology TAIWAN
Introduction of Development Center for Biotechnology TAIWAN DCB Nonprofit Organization Founded in 1984 Funded Mainly by Ministry of Economic Affairs (MOEA), National Science Council and the Industry 394
More informationSartorius Stedim Cellca. Custom Cell Line & Process Development for Pharmaceutical Purposes
Sartorius Stedim Cellca Custom Cell Line & Process Development for Pharmaceutical Purposes May 2017 Overview of Contents 1 2 3 Cellca History Overview: Cellca Technology Platform Products & Services 4
More informationEffectively utilizing Post Translational Modification analysis to fast track process development for Biosimilars
Effectively utilizing Post Translational Modification analysis to fast track process development for Biosimilars Sanjeev Gupta DGM-Advanced Biotech (R&D) Ipca Laboratories Ltd., Mumbai, India March 04,
More informationBIOLOGICS DEVELOPMENT From Discovery to Manufacturing
BIOLOGICS DEVELOPMENT From Discovery to Manufacturing About LakePharma LakePharma is the leading US-based biologics company specializing in antibody and protein, engineering, cell line development and
More informationTransient production of recombinant proteins using the MEXi system
Transient production of recombinant proteins using the MEXi system Dennis Niermeier IBA GmbH Dennis Niermeier 216 Small amounts (mg range) of protein are required in a short time for many experiments and
More informationAAV vectors for gene therapy. Any Gene to Any Cell
AAV vectors for gene therapy Any Gene to Any Cell % Population WHY WORK WITH SIRION? OVERCOME MAJOR ROAD BLOCKS IN AAV GENE THERAPY Invent improved AAV vectors with optimized transduction and expression
More informationThe Trianni Mouse: Best-In-Class Technology for Human Antibody Discovery
The Trianni Mouse: Best-In-Class Technology for Human Antibody Discovery Corporate Overview Co David Meininger, PhD, MBA Chief Business Officer, Trianni 1 Our Mission Trianni is a biotech company with
More informationXpress CF+ : A Cell-Free Platform for the Rapid Screening and Production of Homogeneous ADCs
Xpress CF+ : A Cell-Free Platform for the Rapid Screening and Production of Homogeneous ADCs Alexander R. Steiner, M.S. Director, Protein Biochemistry Tuesday Feb 3 rd, 215 Making novel drugs is Pammolli
More informationNEW! CHOgro Expression System
NEW! CHOgro Expression System At Mirus Bio, we know it s all about expression. Introducing the new CHOgro Expression System, a transient transfection platform that finally gets high protein titers with
More informationSynthetic vaccine research and development. Comprehensive and innovative synthetic biology solutions and technologies
Synthetic vaccine research and development Comprehensive and innovative synthetic biology solutions and technologies From plan to product, Thermo Fisher Scientific supports your synthetic vaccine goals
More informationCustom Antibodies & Recombinant Proteins
Custom Antibodies & Recombinant Proteins INTRODUCTION Custom services to meet your research and development requirements Improvements in health, medicine and diagnostics over the past century can be largely
More informationAdvanced Therapeutic Antibody Discovery with Multiplexed Screening
Advanced Therapeutic Antibody Discovery with Multiplexed Screening White Paper Scientists need powerful tools that can deliver results to fully understand the ability of candidate antibodies to interrupt
More informationApplications involving the ViroCyt Virus Counter in the production of various recombinant proteins
Applications involving the ViroCyt Virus Counter in the production of various recombinant proteins Chris Kemp Kempbio, Inc. Frederick, MD USA chris.kemp@kempbioinc.com Presentation Summary Kempbio, Inc.
More informationLAMPIRE Hybridoma Project Initiation Form
1. General Information Company / Institution: Investigator Name: Contact Name: Phone: Fax: 2. Billing / Shipping Information Accounts Payable contact: Company: Dept./Bldg./Room#: Address: Project Name
More informationMolecular Biology Services. Make Research Easy
Molecular Biology Services Make Research Easy Gene-on-Demand Technology Platform Any gene conceivable in any vector you desire GenScript s Gene-on-Demand technology platform combines patented OptimumGene
More informationExceptional Human Antibody Discovery. Corporate Overview
Exceptional Human Antibody Discovery Corporate Overview Co 1 1 Our Mission Trianni is a biotech company with the scientific mission of creating optimized and highly versatile platforms for generation of
More informationImplementation of a Micro Bioreactor System for Platform Cell Culture Process Development at Cobra Biologics
Implementation of a Micro Bioreactor System for Platform Cell Culture Process Development at Cobra Biologics Kristina Lae, Scientist, Cell Culture Cobra Biologics, Södertälje, Sweden Cobra Biologics and
More informationDevelop Better Assays for Every Human Protein
Develop Better Assays for Every Human Protein OriGene overview Application of transfected over-expression lysates Application of purified recombinant human proteins About OriGene Started in1996 with the
More informationHOST CELL PROTEIN & BIOPROCESSING REAGENT DEVELOPMENT
HOST CELL PROTEIN & BIOPROCESSING REAGENT DEVELOPMENT INTRODUCTION Biopharmaceuticals require products to be free of residual host cell protein (HCP) contaminants from the bioprocessing workflow. To evaluate
More informationProCode TM. Life Science, Inc. A Rapid Flexible MAb-Like Discovery Platform for Creating Diagnostic Antibodies.
ProCode TM A Rapid Flexible MAb-Like Discovery Platform for Creating Diagnostic Antibodies Life Science, Inc. www.meridianlifescience.com Custom Monoclonal Development Meridian Life Science, Inc. (MLS)
More informationMATF Antigen Submission Details and Standard Project Deliverables
MATF Antigen Submission Details and Standard Project Deliverables What we require when you submit your antigen: Proteins For a recombinant protein target, we require a minimum of 400µg soluble recombinant
More informationCustom Antibodies Services. GeneCust Europe. GeneCust Europe
GeneCust Europe Laboratoire de Biotechnologie du Luxembourg S.A. 2 route de Remich L-5690 Ellange Luxembourg Tél. : +352 27620411 Fax : +352 27620412 Email : info@genecust.com Web : www.genecust.com Custom
More informationChallenges in the cgmp Manufacturing of hescs: Lessons Learned from Monoclonal Antibodies
Challenges in the cgmp Manufacturing of hescs: Lessons Learned from Monoclonal Antibodies ISCT 2011 Annual Meeting Rotterdam, The Netherlands May 18 21, 2011 BioProcess Technology Consultants www.bptc.com
More informationClonePix 2. Screen and select more clones in less time. Genetix Now part of Molecular Devices.
ClonePix 2 Screen and select more clones in less time www.moleculardevices.com/genetix Genetix Now part of Molecular Devices Screen more clones in less time Cut cell line and antibody development times
More informationProduct Data Sheet - ANTIBODY
888.267.4436 techsupport@origene.com www.origene.com Name:Mouse Monoclonal MBP Antibody (2H9) Product Data Sheet - ANTIBODY Catalog: TA336919 Components: Mouse Monoclonal MBP Antibody (2H9) (TA336919)
More informationBuilding DNA Libraries to Explore the Combinatorial Design Space. Dr. Yifan Li Senior Scientist GenScript USA Inc.
Building DNA Libraries to Explore the Combinatorial Design Space Dr. Yifan Li Senior Scientist GenScript USA Inc. Outline Combinatorial Optimization for Metabolic Pathway Engineering Case Studies for Combinatorial
More informationOpportunities for Accelerating Cell Line Development and Beyond
Opportunities for Accelerating Cell Line Development and Beyond European CM&C Strategy Forum May 24, 2017 Christopher Frye, Ph.D. Research Advisor & Group Leader Bioprocess R&D Presentation Outline CM&C
More information博奥信生物技术 ( 南京 ) 有限公司. Biosion (China) Recruiting
博奥信生物技术 ( 南京 ) 有限公司 www.biosion.com Biosion (China) Recruiting Who Are We? Biosion is a Nanjing-based biotech company focusing on the discovery and preclinical development of innovative biologicals for
More informationGala s Gene Product Expression (GPEx ) Platform
Gala Biotech A Company with Gene Insertion and Manufacturing Technologies for the Next Generation of Gene Expression and Biologics Production Gala s Gene Product Expression (GPEx ) Platform Rapid creation
More informationMake Your Immunology Research Easy. Kun YIN Associate Director of Marketing Division, GenScript
Make Your Immunology Research Easy Kun YIN Associate Director of Marketing Division, GenScript GenScript Overview Branch Office Amsterdam, Netherlands Branch Office Tokyo, Japan Headquarter, New Jersey,
More informationRisk Assessments for Host Cell Protein Control Strategies: CDER Experiences
Risk Assessments for Host Cell Protein Control Strategies: CDER Experiences Laurie Graham FDA/CDER Office of Pharmaceutical Quality (OPQ) Office of Policy for Pharmaceutical Quality (OPPQ) 1 Disclaimer
More informationTake the Rational Approach of the BalanCD CHO Media Platform
Accelerate your development of therapeutics from discovery to commercialization Take the Rational Approach of the BalanCD CHO Media Platform It takes ambition, dedication, and fortitude to translate a
More informationCHOgro Expression System
CHOgro Expression System At Mirus Bio, we know it s all about expression. Introducing the new CHOgro Expression System, a transient transfection platform that finally gets high protein titers with robust
More informationINTEGRATED PRODUCTION PLATFORMS
FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME PROCESS DEVELOPMENT, SCALE UP, GMP-COMPLIANT PRODUCTION AND CONSULTING INTEGRATED PRODUCTION PLATFORMS EVERYTHING STARTS WITH YOUR BRILLIANT
More informationPharma&Biotech. Developability Assessment Platform Reduce Attrition Rates, Improve Clinical Safety and Avoid the Valley of Death
Pharma&Biotech Developability Assessment Platform Reduce Attrition Rates, Improve Clinical Safety and Avoid the Valley of Death The Valley of Death Development risk, safety and high attrition rates remain
More informationDon Stewart, PhD President and CEO (416)
PlantForm Corporation Don Stewart, PhD President and CEO (416) 452 7242 don.stewart@plantformcorp.com www.plantformcorp.com PlantForm Opportunity Proprietary, plant-based manufacturing of monoclonal antibody
More informationAntibody Discovery at Evotec
Antibody Discovery at Evotec - Overview - Evotec Antibodies Adding value to our partners research Innovative and flexible solutions from target ID to pre-clinical candidate The people A wide therapeutic
More informationProduct Data Sheet - TRUEMAB
888.267.4436 techsupport@origene.com www.origene.com Name:KRT7 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) Product Data Sheet - TRUEMAB Catalog: TA802932 Components: KRT7 mouse monoclonal antibody,
More informationJob Title: Cell Culture Technician (open June 1 through Oct 31, 2016)
Rockland Immunochemicals Inc. is recognized as one of the world's leading manufacturers and suppliers of immunochemical reagents for basic research and discovery. Rockland Immunochemicals provides reagents
More informationDevelopment. - Be Your Partner-
Development - Be Your Partner- 1 Product development and management 2 Product development and management A good product development is based on a strong product management. Mycenax sproject management
More informationLFB BIOMANUFACTURING YOUR CDMO PARTNER FOR MABS AND RECOMBINANT PROTEINS B IOMANUFACTURING
YOUR CDMO PARTNER FOR MABS AND RECOMBINANT PROTEINS B IOMANUFACTURING COMMITMENT AND FLEXIBILITY LFB BIOMANUFACTURING is a medium-sized CDMO offering Bioproduction services, from an industrial facility
More informationMonoclonal Antibody Generation. Ivo Lorenz Tri-Institutional Therapeutics Discovery Institute
Monoclonal Antibody Generation Ivo Lorenz Tri-Institutional Therapeutics Discovery Institute Common Antibody Formats Heavy chain VL VH Light chain Fab CL CH1 VH VL Linker CH2 VH VL Fc CH3 IgG Immunoglobulin
More informationPersonalized CAR-T Immunotherapy Platform
GLP, GMP, and CLIA-Certified Lab Personalized CAR-T Immunotherapy Platform Accelerate your cancer research and drug discovery Platform Overview 1500 Existing Hybridomas and Antibody Engineering Custom
More informationManufacturing Integrated Biologics Manufacturing
Manufacturing Integrated Biologics Manufacturing Integrated Biologics Manufacturing Abzena provides customer focused process development and manufacturing services for cgmp and non-gmp production of recombinant
More informationUNLEASH THE POWER OF PRECISION MEDICINE
FROM BENCH TO BEDSIDE UNLEASH THE POWER OF PRECISION MEDICINE DCB, pioneer in biotech. Your partner in drug discovery. www.dcb.org.tw The Center for Biotechnology With over 400 dedicated researchers and
More informationProtein or Peptide Antigen?
Protein or Peptide Antigen? Liyan Pang, Ph.D. liyan.pang@genscript.com Table of Contents 1 2 3 4 5 6 Choosing mab or pab Characteristics of a Good Antigen Protein Antigen Peptide Antigen How to Choose
More informationThe SMARTag TM ADC Technology Platform
The SMARTag TM ADC Technology Platform 2 3 4 World Class Protein Production Capability New State of the Art Facility Madison, WI Expanded GPEx Cell Line Engineering capacity Flexible Non cgmp production
More informationOptimization of Protein Purification Using Small-Scale Separation Columns
Optimization of Protein Purification Using Small-Scale Separation Columns Chris Suh, Ph.D Application Scientist, Inc. San Jose, Ca 2 nd Therapeutics & Drug Discovery Conference Presentation Outline 1.
More informationThermo Scientific Metabolic Pathway Design Process Cell culture media and process optimization approaches for optimal biotherapeutic production
Thermo Scientific Metabolic Pathway Design Process Cell culture media and process optimization approaches for optimal biotherapeutic production May/June 2013 1 The world leader in serving science Agenda
More informationAntibody-Drug Conjugate Characterization and Quality Assurance
Antibody-Drug Conjugate Characterization and Quality Assurance Sarah Kennett Division of Monoclonal Antibodies Office of Biotechnology Products OPS/CDER/FDA October 12, 2011 1 Disclaimer The views and
More informationLinker p. 177 Helper Lipid p. 178 Delivery to Target Cells p. 180 Cell Entry p. 182 Receptor-Mediated Uptake p. 182 Endosomai Release p.
Overview of Regulatory Expectations for Introducing Novel Therapies into Clinical Trials Introduction p. 1 Roles of Regulatory Scientists p. 2 Product Development and Availability p. 2 Data Requirements
More informationProtein Purification Products. Complete Solutions for All of Your Protein Purification Applications
Protein Purification Products Complete Solutions for All of Your Protein Purification Applications FLAG-Tagged Protein Products EXPRESS with the pcmv-dykddddk Vector Set Fuse your protein of interest to
More informationYour Antibody Source. prosci-inc.com. Extensive Antibody Services Broad Antibody Catalog
Your Source prosci-inc.com Extensive Services Broad Catalog Company Overview Established in 1998, ProSci Incorporated is a leading provider of high performance antibodies and custom antibody services.
More informationCell Line Characterization and Product Testing for CHO-Derived Biopharmaceutical Products
Cell Line Characterization and Product Testing for CHO-Derived Biopharmaceutical Products Founded in 96, Eurofins Lancaster Laboratories is a global leader in comprehensive laboratory services enabling
More informationHYPE-CHO Transfection Kit Results
HYPE-CHO Transfection Kit Results HYPE-CHO Transfection Kit is the newest reagent dedicated to achieve High Yield Protein Expression in CHO cells. This kit has been designed for maximum efficiency in CHO
More informationICANtibodies TM. Has discovered more than 200 different antibodies for less than 2 years.
An innovative antibody development technology using breakthrough nanotaxis leading to the antigen expression by the chosen animal species. Has discovered more than 200 different antibodies for less than
More informationCONTRACTING ORGANIZATION: Albert Einstein College of Medicine Bronx, NY 10461
AD Award Number: W81XWH-08-1-0011 TITLE: Defining B. Anthracis Protective Antigen Antigenic Domains PRINCIPAL INVESTIGATOR: Arturo Casadevall, M.D., Ph.D. CONTRACTING ORGANIZATION: Albert Einstein College
More informationPhage Antibody Selection With Reichert SPR System
Phage Antibody Selection With Reichert SPR System Reichert Technologies Webinar April 4, 2016 Mark A. Sullivan, Ph.D. Department of Microbiology and Immunology University of Rochester Presentation Outline
More informationThe Elite Provider. Cell & Gene. Therapy Manufacturing
The Elite Provider Cell & Gene Therapy Manufacturing GMP Manufacturing Services The cgmp manufacturing in Vigene is compliant with US FDA and EU EMA regulatory requirements. The manufacturing area consists
More informationChallenges in Scaling Up Newly Developed Microbial Manufacturing Processes
Challenges in Scaling Up Newly Developed Microbial Manufacturing Processes Susan Dana Jones, Ph.D. BioProcess Technology Consultants BIO Process Zone Theater June 19, 2008 Microbial Process Development
More informationVaccines based on Recombinant Proteins and Adjuvant Systems: GSK's malaria vaccine candidate as a case study.
Vaccines based on Recombinant Proteins and Adjuvant Systems: GSK's malaria vaccine candidate as a case study. CMC Forum Vienna May 25-27 Vaccine workshop M.-C. Uwamwezi, Senior scientist Regulatory Affairs,
More informationProduct Data Sheet - TRUEMAB
888.267.4436 techsupport@origene.com www.origene.com Name:VAPA mouse monoclonal antibody, clone OTI10E10 (formerly 10E10) Product Data Sheet - TRUEMAB Catalog: TA809100 Components: VAPA mouse monoclonal
More informationAutomated Electrophoresis Applications for Synthetic Biology
Automated Electrophoresis Applications for Synthetic Biology Melissa Huang Liu, Ph.D. Product Manager, Electrophoresis Agilent Technologies 1 Outline 2100 Bioanalyzer & 4200 TapeStation Systems Automated
More informationFDA HCP assays are current the weakest analytical link in the total control strategy for biotech products
FDA HCP assays are current the weakest analytical link in the total control strategy for biotech products What are the best ways to prepare antigen/antibody reagents with highest HCP coverage? There was
More informationHigh-Throughput Screening for Antibody Discovery Using Mirrorball
High-Throughput Screening for Antibody Discovery Using Mirrorball Christyne Kane Senior Scientist I AbbVie Bioresearch Center Antibody Engineering & Therapeutics December 12, 2017 Christyne Kane is an
More informationThe World Leader in SPR Technology. Jimmy Page, PhD, Biacore, Inc.
The World Leader in SPR Technology Jimmy Page, PhD, Biacore, Inc. Objectives of Biacore Experiments Yes/No Data» Is there binding?» Ligand Fishing Concentration Analysis: How MUCH? Active Concentration
More informationExamining the components of your peptide sample with AccuPep QC. Lauren Lu, Ph.D. October 29, 2015, 9:00-10:00 AM EST
Examining the components of your peptide sample with AccuPep QC Lauren Lu, Ph.D. October 29, 2015, 9:00-10:00 AM EST When do I need custom peptides? Custom peptides play an important role in many research
More informationEvolving of Biological Product Expression Systems with Host Cell Engineering
Evolving of Biological Product Expression Systems with ost Cell Engineering Lianchun Fan Bristol-Myers Squibb CMC Strategy Forum Jan. 23rd, 2017 Outline Choice of expression systems New technologies and
More informationPurification of alpha-1 antitrypsin using an antibody based affinity chromatography medium
Purification of alpha-1 antitrypsin using an antibody based affinity chromatography medium Ulrika Meyer a, Hanna Wlad a, Sven Blokland b, Frank J.M. Detmers b and Henrik Ihre a a GE Healthcare Bio-Sciences
More information