impact Innovation with Integrity Maximum Impact Definitive Answers UHR-TOF MS

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1 Maximum Impact Definitive Answers impact Innovation with Integrity UHR-TF MS

2 Redefining Accurate Mass LC-MS/MS Until now, mass spectrometry technologies have forced scientists to choose between performance characteristics for a given application. ften, a system is designed for qualitative work, sacrificing quantitation performance in the process. There is no need to make compromises in mass spectrometry anymore. The maxis impact sets a new technology standard where industry leading performance values are all simultaneously available in a single acquisition at full sensitivity. Powered by a series of patented technology innovations, the maxis impact simply provides the very best results without compromise in a cost effective, benchtop format. maxis impact: Qualitative and Quantitative results from a single LC/MS analysis Sub-ppm mass accuracy and, full sensitivity resolution Extreme sensitivity across the entire mass range from very small molecules to intact proteins High speed MS/MS capability with full U-HPLC compatibility Simultaneous analysis of major and trace sample components Isotopic fidelity for definitive molecular formulae determination Robust and easy to operate system And all in an economical and compact design!

3 Unparalleled Performance Now in a Bench-top Package Dual Stage Reflector High Transmission CID Cell Flight Tube with in-flight focussing for full sensitivity resolution ESI Source Patented Dual Ion Funnel Quadrupole Long Life Flash Detector The only no-compromise full sensitivity and full resolution UHR-TF on the market A game changing step forwards in TF technology featuring nine new patents. The maxis impact is powered by a number of outstanding technology innovations including: Unique Full Sensitivity Resolution (FSR) technology in all modes Proven Bruker UHR-TF technology in bench-top format New broadband transmission CID cell providing ground-breaking sensitivity Fast G sample/sec digitizer enabling data acquisition at up to 5 Hz Long life FLASH Detector Flexible choice of API sources including new CaptiveSpray TM and solid sample (DIP) and GC inlets Refining capabilities across the analytical world With it s unique capabilities, the maxis impact liberates your work from restriction and compromise. Whether the need is for small or large molecule analysis, quantitation or molecular identification, the maxis impact is more than capable of providing the results needed to make decisions first time, every time. Let the maxis impact achieve your goals in: Forensics, toxicology & doping control Food & environmental testing Synthetic chemistry support Drug metabolite and impurity identification and quantitation Metabolomics Intact protein analysis & characterization of biophamaceuticals Biomarker discovery & validation

4 ne-shot Certainty for Qual/Quant Analysis maxis impact captures the full picture of your sample in one analysis alleviating the need for multiple runs. More than orders of fundamental dynamic range achieved without artificial compensators allows simultaneous measurement of major and trace sample components in a single analysis - even if coeluent. Area Buspirone MS/MS linearity.5 to 5 ppb.e+7.5e+7.e+7 5.E+6 Buspirone MSMS linearity y = x R =.999 LD =.5 ppb ( fg absolute on column).e LD =.5 ppb ( fg absolute on column) Qualitative and quantitative linear range from.5 results to 5 ppb from (R =.999) a single auto MS/MS run conc [ppb] Ultra fast acquisition speed up to 5 Hz m/z Mass accuracy better be er than ppm Resolution Resolu on 5k-k 5Hz 5Hz Hz 5Hz Hz 5Hz Hz 5Hz Hz 5Hz Hz MS of Buspiron acquired at Hz up to 5 Hz spectra to disk. Resolution and spectral accuracy maintained even at maximum speed x 6 5 TIC [%] 8. +MS, min #- MS matrix background Time [min] m/z x hrxic 5 mda selectivity [%] MS(.75),.eV, min #5-5 6 MS/MS Isotopes Time [min] m/z x hrxic data points MS and MS/MS zoomed hrxic Time [min]. TIC from complex sample (6 pesticides spiked into ginger extract (QuEChERS)).. High resolution extracted ion chromatogram (hrxic) of m/z.75 with 5mDa discrimination width (Acetamiprid).. Magnified LC peak. High speed acquisition rate in MS and MS/MS mode (MS: ms, MS/MS: ms spectra time). data points across this LC peak allows precise quantitation.. High resolution survey MS with m/z.75 selected as precursor. 5. High spectral accuracy means isotopic fidelity for identification certainty. 6. High sensitivity MS/MS spectrum even at very low MW (56.98 m/z) for confirmation by fragment information.

5 Time [min] Precisely Targeted Small Molecule Analysis Ideally suited for broad band multi-target screening Multi-target screening at mda hrxic selectivity x 5 Mix_5ppb_GB_5_997.d Resolve and identify multiple compounds from extremely complex mixtures across a wide mass range even at low analyte concentrations. With these key performance attributes, the maxis impact is an ideal fit for rapid, high target number screening tasks such as food testing or forensic analysis Amitrole, [M+H] +, m/z 85 Avermec n, [M+NH ] +, m/z 89 xamyl, Fragment m/z 7 Spinosad A, [M+H] +, m/z 7 For the laboratory challenged with rapid response to novel analytes, retrospective in-silico screening of complete datasets using TargetAnalysis TM software makes maxis impact the most agile screening tool available Time [min] mda high EIC resolution width mda XIC from pesticides. Each color represents the hrxic of one pesticide. Sample at a concentration of 5 ppb each separated with U- HPLC maxis impact. Triple quad sensitivity and quantitation with high-res, accurate mass orders of magnitude Very low LD 5 fg The maxis impact encapsulates the capabilities of low fg range sensitivity with more than four orders of magnitude dynamic range and quantitative capacity along with the performance of an exceptional high resolution accurate mass instrument. The simultaneous combination of attributes is ideal for many discovery, development, screening and drug metabolism applications which often require both the identification and quantitation of sample components that vary widely in both structure and concentration. Excellent for use in the analysis of both known and unknown compounds, the maxis impact represents a new level of achievement in the challenge to provide one step qualitative and quantitative data in a single rapid analysis. Fluoxastrobin (m/z 59) has been quantified over orders of magnitude in concentration from ng down to 5fg. The linearity is R=.9996 with an ultra low LD coupled with accurate mass certainty.

6 H H H H H H Small Molecule ID & Structure Elucidation Impacting Metabolomics results A key bottleneck in metabolomics research is structural confirmation and elucidation of metabolites. Diterpene glycosides (DTGs) are abundant plant defence compounds with largely unknown modes of action. utstanding MS and MS/MS data for Nicotiana attenuata (tobacco) DTGs acquired on a maxis impact enabled the rapid identification of the entire compound family. To achieve this, the fragmentation results are visualized and interpreted using Bruker s novel FragmentExplorer TM. The FragmentExplorer has been especially designed for faster interpretation of MS/MS data. It provides an interactive link between SmartFormula D TM results, mass spectra and molecular structures. Compound identification and structure confirmation x H H H H MS(99.75) m/z SmartFormulaD TM co-developed with Pfizer, utilizes maxis impact high isotopic fidelity in MS and MS/MS in a unique relational algorythm to assign molecular formulae of unknown analytes without prior knowledge or assumptions. SmartFormulaD FragmentExplorer C H, -. mda, 7. C 5 H 9,.5 mda, C 5 H,.6 mda, C 5 H,. mda, 95.8 C 5 H 5,.5 mda, C 6 H,. mda, C 8 H 5,.7 mda, C 8 H 5 6,. mda, C 9 H 8,. mda, C 9 H 5 9,.6 mda, C H 8,.8 mda, C H 9,.5 mda, C H 5,. mda, C 5 H 55,.7 mda, C H 7,.5 mda, C H 9,.8 mda, C H 69,.8 mda, C H, -.5 mda, C 6 H 9,. mda, FragmentExplorer with embedded ChemDraw TM expertly assists fragment assignments.

7 Biomarker Discovery and Validation In-depth qualitative analysis of complex proteomics samples ID more than 5 proteins with FDR <% Get full performance first time, every time The CaptiveSpray is a revolutionary LC-MS source that combines the sensitivity of nanospray with the ease of use and robustness of electrospray. CaptiveSpray utilizes patented technology to capture and sweep sample ions into the MS independent of LC flow rate. LC auto MS/MS survey. The human cell line HT9 is an established model system to study colon cancer progression. A µg digest of HT9 cells was separated on a RSLCnano system ( h gradient, 5 cm column) and measured on a maxis impact with a captive spray source. CaptiveSpray LC-MS source Identification over a dynamic range of decades of concentration from complex samples Real-life MS mass accuracy decades of concentration. pmol fmol fmol. fmol +/-.6 ppm Sigma UPS- standard sample Plot of all peptides from a digest of ng E.coli

8 Bruker Daltonics is continually improving its products and reserves the right to change specifications without notice. BDAL 6-, #7678 BioPharma QC x 5 5 x 5 5 Isotopic resolution of intact proteins Beta lactoglobulin B, (pmol/µl infusion) ' MS,.-7.5min, Deconvoluted (MaxEnt) MS spectrum MaxEnt -deconvoluted of Beta lactoglobulin B (Infusion of pmol/µl) with fully resolved isotopes C87HN68S9, M+nH,865.9 Simulated spectrum with a, Resolu on resolution, Quantum leap in data quality for biopharmaceutical characterization With k-k resolution for intact full sensitivity with true isotopic patterns, the maxis impact allows you to simultaneously characterize, impurity detect and quantify your biopharmaceutical products. Combined with MS& MS/MS accuracy < ppm the maxis impact ensures certainty first time every time, allowing you to report your results with confidence m/z Source ptions Wide choice of ionization and coupling techniques for a broad range of sampling including insoluble compounds: GC-MS coupling APCI II (atmospheric pressure chemical ionization) source with direct probe APPI II (atmospheric pressure photo ionization) source CaptiveSpray LC-MS source CE-MS coupling with grounded needle GC-MS coupling Solid probe mounted on an APCI II source LC ptions Bruker fully supports and integrates a wide range of leading HPLC systems, autosamplers and accessories. APPI II source For research use only. Not for use in diagnostic procedures. Bruker Daltonik GmbH Bremen Germany Phone +9 ()-5- Fax +9 ()-5- sales@bdal.de Bruker Daltonics Inc. Billerica, MA USA Fremont, CA USA Phone + (978) Phone + (5) 68- Fax + (978) Fax + (5) ms-sales@bdal.com ms-sales@bdal.com