Synchronised solutions for cell culture

Transcription

1 GE Healthcare Life Sciences Synchronised solutions for cell culture Choose Whatman consumables for quality and knowledgeable support from GE Healthcare

2 Synchronised solutions for cell culture Whether you culture millilitres or litres of cells, GE Healthcare Life Sciences has Whatman products for you. See the workflows below to identify appropriate products for your needs. SMALLER VOLUMES Puradisc sterile syringe filter Lens cleaning tissue for optical lenses BugStopper venting device Filtration of culture media & additives (p. 2 3) Cell observation (p. 8 9) Venting during cell growth (p. 4 5) Clarification of cultures (p. 6 7) Nuclepore membrane HEPA-VENT glass fibre disc filter GD/X sterile multiple-layer syringe filter, PES

3 Our filters and other devices enable you to filter media, grow cells, clarify cultures, and observe cells. Klari-Flex bottle top filter LARGER VOLUMES Lens cleaning tissue for optical lenses Filtration of culture media & additives (p. 2 3) HEPA-CAP capsule filter Cell observation (p. 8 9) Venting during cell growth (p. 4 5) Clarification of cultures (p. 6 7) Nuclepore membrane Polycap TC PES capsule filter 1

4 Filtration of culture media and additives Volumes up to 100 ml Puradisc syringe filters Whatman Puradisc syringe filters from GE Healthcare are available with a wide selection of sizes and membrane types to provide full flexibility for each application. For cold sterilization we recommend the Puradisc sterile syringe filter with a 0.2 µm membrane. The membrane (polyethersulfone or cellulose acetate) is inherently hydrophilic, has low extractables, and exhibits low protein binding. These features allow samples to be filtered without any risk of contamination. The housing is made of pigment-free material ensuring high-quality sample preparation for critical applications. Two options are available: Puradisc 25 mm, with polyethersulfone (PES) membrane Puradisc FP 30 mm, with cellulose acetate (CA) membrane Puradisc features: Clean room manufacturing/production Pore sizes of 0.2 µm and 0.45 µm Nonsterile options available Other membrane types available Fig 1. Puradisc syringe filters. Puradisc syringe filters, sterile Puradisc FP 30: 0.2 µm, 30 mm diam, CA membrane, polycarbonate housing 50 per pack Puradisc FP 30: 0.45 µm, 30 mm diam, CA membrane, polycarbonate housing Puradisc 25: 0.2 µm, 25 mm diam, PES membrane, polypropylene housing Puradisc 25: 0.45 µm, 25 mm diam, PES membrane, polypropylene housing 50 per pack per pack per pack Volumes up to several litres Polycap AS capsule filters The Polycap AS capsule filter is recommended for the filtration of aqueous solutions. It combines a glass microfibre (GMF) prefilter and a nylon membrane, to create a high performance capsule that improves flow rates and prolongs filter life. Users can filter large volumes (up to 20 l) and difficult samples more easily. Polycap capsules can filter buffers or heavily loaded biological solutions including virus suspensions, biological suspensions, admixtures, and enzymes. Different capsule sizes are available with a choice of inlet/ outlet connections. Product In* Out* Filtration area Quantity Code no. Polycap 36 AS 0.2 µm nylon, sterile ½ SB ½ SB 400 cm 2 1 per pack µm nylon, sterile SB SB 400 cm 2 1 per pack µm nylon, sterile SB SB 400 cm 2 1 per pack Polycap 36 AS with filling bell 0.2 µm nylon, sterile SB SB 400 cm 2 1 per pack Polycap 75 AS 0.2 µm nylon, sterile ½ SB ½ SB 820 cm 2 1 per pack Fig 2. Polycap 36 AS with filling bell. 0.2 µm nylon, sterile SB SB 820 cm 2 1 per pack µm nylon, sterile ½ SB ½ SB 820 cm 2 1 per pack Polycap 75 AS with filling bell 0.2 µm nylon, sterile SB SB 820 cm 2 1 per pack Polycap 150 AS 0.2 µm nylon, sterile ½ HB ½ HB 1650 cm 2 5 per pack µm nylon, sterile ½ SB ½ HB 1650 cm 2 5 per pack 2807 * SB = stepped barb for 6 to 10 mm tubing (¼" to ⅜" ); ½ SB = stepped barb for 10 to 12 mm tubing (⅜" to ½"); ½ HB = hose barb Other pore sizes and connections are available. For more information see or contact whatmaninformation@ge.com.

5 Volumes of 250 to 1000 ml Klari-Flex filtration system Whatman Klari-Flex Bottle-Top Filtration System provides an easy-to-use stand-alone filtration device that improves overall efficiency and minimises the risk of spills and contamination. Klari-Flex includes the low protein binding PES membrane. Klari-Flex system features Drop-Connect Technology, which requires just one hand to connect the unit to the base. There is no need to detach/attach tubing because the vacuum can be controlled using the vacuum knob. Product is gamma sterile and individually bagged. Connect vacuum to the docking station only once and plug in the Klari-Flex filtration unit Pour in your culture media or additives, close the lid and... start filtration by turning on the knob on the docking station Remove the bottle, close it with the cap done! Fig 3. Klari-Flex Bottle-Top Filtration System is available in three sizes. Fig 4. Klari-Flex system in use. Klari-Flex Bottle-Top Filtration System, sterile Klari-Flex Bottle Top 250 ml, 0.22 µm PES (complete unit) 12 per pack Klari-Flex Bottle Top 250 ml, 0.45 µm PES (complete unit) 12 per pack Klari-Flex Bottle Top 500 ml, 0.22 µm PES (complete unit) t12 per pack Klari-Flex Bottle Top 500 ml, 0.45 µm PES (complete unit) 12 per pack Klari-Flex Bottle Top 1000 ml, 0.22 µm PES (complete unit) 12 per pack Klari-Flex Bottle Top 1000 ml, 0.45 µm PES (complete unit) 12 per pack Klari-Flex Filter Funnel 250 ml, 0.22 µm PES 12 per pack Klari-Flex Filter Funnel 250 ml, 0.45 µm PES 12 per pack Klari-Flex Bottle-Top Filtration System, sterile cont. Klari-Flex Filter Funnel 500 ml, 0.22 µm PES 12 per pack Klari-Flex Filter Funnel 500 ml, 0.45 µm PES 12 per pack Klari-Flex Filter Funnel 1000 ml, 0.22 µm PES 12 per pack Klari-Flex Filter Funnel 1000 ml, 0.45 µm PES 12 per pack Klari-Flex Bottle 1000 ml 24 per pack Klari-Flex Bottle 250 ml 24 per pack Klari-Flex Bottle 500 ml 24 per pack Klari-Flex Cradle Ring 1 per pack Klari-Flex Docking Station 1 per pack Glass microfibre prefilters GMF µm, 66 mm disc for 250 ml unit 20 per pack GMF µm, 90 mm disc for 500 ml and 1000 ml units 20 per pack

6 Venting during cell growth Standard flasks (125 to 2500 ml) BugStopper sterile closure devices BugStopper devices prevent bacteria and viruses from entering or exiting a culture vessel, while allowing air and gas to pass through the membrane. BugStopper devices (BugStopper and BugStopper 10) will withstand 50 standard autoclave cycles without loss of performance. Repeated use reduces cost per use significantly. Needles may be used to penetrate the silicone portion of the BugStopper device for use as a sample port or for gas infusion. This is not recommended for BugStopper 10 because of the wall thickness. BugStopper/BugStopper 10 devices are easy to use; you simply push the device into the neck of the flask and autoclave the entire vessel. BugStopper Stainless steel reinforcement ring surrounds the media for added support Vent is composed of hydrophobic ultrafine glass microfibre media reinforced with a polyester monofilament layer Fig 5. BugStopper 10 (left) and BugStopper (right). BugStopper 10 Biosafe silicone wall Stainless steel reinforcement ring surrounds the media for added support Vent is composed of hydrophobic ultrafine glass microfibre media reinforced with a polyester monofilament layer Integral silicone shroud to keep flask lip clean Biosafe silicone wall Fig 6. Schematic diagram showing design features of BugStopper and BugStopper 10. Fig 7. BugStopper device in use. BugStopper 10 per pack BugStopper per pack

7 Alternative culture vessels HEPA-VENT and HEPA-CAP filters The design of these HEPA depth filters gives a high flow rate and low pressure drop, ensuring that clean air passes into and out of vessels. HEPA-VENT and HEPA-CAP prevent bacterial, algal, or fungal contamination in fermentors and incubators and are suitable for particle removal from air and gases. These filters can be sterilized by autoclaving with steam. Product In* Out* Filtration area Quantity Code no. HEPA-VENT and HEPA-CAP filters HEPA-VENT 50 mm disc SB SB 16 cm 2 10 per pack HEPA-CAP 36 capsule SB SB 625 cm 2 1 per pack HEPA-CAP 75 capsule ½ SB ½ SB 1300 cm 2 1 per pack * SB = stepped barb for 6 to 10 mm tubing (¼" to ⅜"); ½ SB = stepped barb for 10 to 12 mm tubing (⅜" to ½") Fig 8. HEPA-VENT disc and HEPA-CAP capsule filters. 5

8 Clarification of cultures Syringe filters for smaller volumes Whatman GD/X syringe filters Whatman GD/X syringe filters contain multiple glass microfibre prefilters in addition to a layer of PES membrane. Filtering suspensions that contain a high loading of particulate matter becomes easier and a higher sample volume can be filtered than with standard syringe filters. GD/X features: Low protein binding PES membrane Pigment-free polypropylene (PP) housing Pore sizes of 0.2 μm and 0.45 μm Nonsterile options available Other membrane types available GD/X syringe filters contain four filtration layers which substantially reduces blockage and increase volume throughput Sample flow Layers 1 and 2 Glass microfibre prefilter GMF 150 (graded density) filters from 10 µm to 1 µm Fig 10. Schematic diagram showing layers of GD/X filters. Layer 3 Glass microfibre prefilter GF/F filters down to 0.7 µm Layer 4 Membrane filter PES, etc. Filters down to 0.45 µm or 0.2 µm Fig 9. Detailed schematic diagram of GD/X filter layers. GD/X µm PES GMF, sterile 50 per pack GD/X µm PES GMF, sterile 50 per pack Other membranes, pore sizes, and dimensions are available. For more information see or contact whatmaninformation@ge.com. Fig 11. Whatman GD/X filter in use. 6

9 Capsule filters for larger volumes Polycap TC capsule filters Critical filtrations of virus suspensions and cell culture media require outstanding products that do not contaminate the sample. The PES filtration membrane, together with the test methods used during the manufacturing process, ensure that Polycap TC capsule filters are one of the most suitable capsules for these applications. Polycap TC capsule filters are disposable, dual-layer PES membrane filtration capsules that provide efficient filtration for critical aqueous solutions. The PES membrane is inherently hydrophilic, has excellent flow rates, has low extractables, is biosafe, and exhibits low protein binding. Polycap TC 36 has a filtration area of 400 cm 2. Product In * Out * Quantity Code no. Polycap 36 TC 0.2/0.1 µm PES, sterile SB SB 1 per pack /0.1 µm PES, sterile SB SB 1 per pack /0.1 µm PES, sterile ½ SB ½ SB 1 per pack /0.45 µm PES, sterile SB SB 1 per pack Polycap 36 TC with filling bell 0.2/0.1 µm PES, sterile SB SB 1 per pack /0.1 µm PES, sterile SB SB 1 per pack /0.45 µm PES, sterile SB SB 1 per pack * SB = stepped barb for 6 to 10 mm tubing (¼" to ⅜"); ½ SB = stepped barb for 10 to 12 mm tubing (⅜" to ½") Fig 12. Polycap TC capsule filter range. Other capsule sizes, pore sizes, and connections are available. For more information see or contact whatmaninformation@ge.com. Negative pressure Positive pressure Polycap Vacuum Polycap VACU-GUARD Peristaltic Pump Fig 13. Options for connecting a Polycap capsule filter. 7

10 Cell observation Critical optical measurements Glass bottom microplates Optically clear glass bottom microplates are suitable for use in a wide range of critical optical measurement techniques including enzyme studies, cell-based assays, and DNA-protein interaction. The plates are available with a choice of clear or black polystyrene plate bodies that have a premium quality slip glass of a uniform mm thickness. The uniformity of the glass is important to ensure a uniform background for all wells in the plate. Black plate bodies isolate the excitation or emission energy to the individual wells so that there is no interference from well to well. This can be important for sensitive probes, light-sensitive cells, and background subtraction. A further modification to the plate, which enhances its use for these applications, is the inclusion of a low profile skirt. Normally the microplate skirt lifts the well base slightly. In this case the skirt isolates the wells from outside light energy while allowing direct contact of the glass bottom with the optics of the microscope or reader. Glass bottom microplates Tissue culture treated, irradiated with lid, standard skirt 96 wells, 300 µl, black polystyrene 5 per pack Tissue culture treated, irradiated with lid, skirtless for microscopy 96 wells, 300 µl, black polystyrene 5 per pack Fig 14. Clear and black glass bottom microplates. No surface treatment, standard skirt 96 wells, 300 µl, black polystyrene 5 per pack No surface treatment, skirtless for microscopy 96 wells, 300 µl, clear polystyrene 5 per pack wells, 300 µl, black polystyrene 5 per pack Fig 15. Skirtless glass bottom microplate on viewing surface (grey). 8

11 Cell growth and observation Nuclepore membranes Cells can be readily grown on a matrix. This matrix needs to be a solid substrate that does not interact with cell growth and permits easy transportation of liquid throughout the substrate into the cells. A suitable matrix for this application is a polycarbonate membrane such as Nuclepore. Nuclepore membranes are manufactured without the use of surface tension improvers, so extractables are low and do not interact with cells during the growth process. Nuclepore membranes have a smooth and flat surface offering good visibility of cells during and after cell culture. Simply place your Nuclepore membrane on the culture media and spike your cells on the surface. Fig 16. Nuclepore membrane. Nuclepore 13 mm, 3.0 µm circle 100 per pack Nuclepore 13 mm, 5.0 µm circle 100 per pack Nuclepore 13 mm, 8.0 µm circle 100 per pack Nuclepore 13 mm, 5.0 µm, PVP-free circle 100 per pack Nuclepore 13 mm, 8.0 µm, PVP-free circle 100 per pack Nuclepore 25 mm, 2.0 µm circle 100 per pack Nuclepore 25 mm, 3.0 µm circle 100 per pack Nuclepore 25 mm, 5.0 µm circle 100 per pack Nuclepore 25 mm, 8.0 µm circle 100 per pack Nuclepore mm, 8.0 µm sheet 100 per pack Nuclepore mm, 5.0 µm, PVP free sheet 100 per pack Nuclepore mm, 8.0 µm, PVP free sheet 100 per pack Cleaning optical lenses Lens cleaning tissue This high-quality Whatman lens cleaning tissue ensures the safe removal of surface moisture or grease. The tissue provides a very soft surface. It is chemically pure and free from silicones and other additives. Lens cleaning tissue mm, 25 wallets of 25 sheets 25 per pack Fig 17. Lens cleaning tissue. 9

12 For local office contact information, visit: GE Healthcare UK Limited Amersham Place Little Chalfont, Buckinghamshire HP7 9NA, UK All the products presented in this brochure are available from GE Healthcare distributors. GE, imagination at work and GE monogram are trademarks of General Electric Company. BugStopper, Klari-Flex, Nuclepore, Whatman, and Whatman GD/X are trademarks of GE Healthcare companies General Electric Company All rights reserved. First published April AA 04/2011