Clontech Product Selection Guide

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3 Clontech Product Selection Guide PCR THigh Yield - TITANIUM Taq High Yield & Fidelity - Advantage 2 polymerase High Fidelity CloneAmp HiFi Direct PCR- Terra PCR Direct polymearase Transfection Reagent Transfection Reagent Xfect Xfect Protein Transfection Reagent Mouse Embryonic Stem Cells Transfection Reagent (mesc) Xfect Adult Stem Cell Transfection In-Fusion HD PCR Cloning GenomeWalker Kits Genomic Libraries and DNA Site-Directed Mutagenesis Kit sirna Transfection Reagent Xfect Calcium Phosphate Transfection Kits Cell Culture FBS(Tet System Approved) Antibiotics/X-GLUC Magnetic Separator Reverse Transcriptase RNA to cdna EcoDry Premix SMARTScribe RT Fluorescent protein vectors & Antibody AcGFP1 AmCyan1 DsRed2 tdtomato EGFP Monoclonal Antibody Reporter System Great EscAPe SEAP reporter Ready to Glow Secreted Luciferase Luminescent β-gal Reporter ApoAlert Annexin V Apoptosis Kit ApoAlert Caspase Assay Kits & Plates LDH Cytotoxicity Detection Kit Premixed WST-1 Cell Proliferation Reagent Cell Cycle Monitoring Vectors (Fucci)

4 Lentiviral Expression Systems Lentivirus Expression and Packaging Lentivirus Concentration, Purification and Titration Retroviral Expression Systems Retrovirus Expression and Packaging Retrovirus Concentration and Titration SMARTer for Illumina SMARTer cdna Synthsis Kit SMARTer RACE cdna Amplification Kit Marathon cdna Amplification Kit SMART Library construction Kit In-Fusion SMARTer cdna Library construction Kit Adenoviral Expression Systems Adenovirus Expression and Packaging Adenovirus Purification and Titration Mammalian Inducible Expression Tet- Inducible Expression System Tet-Express Inducible Expression System ProteoTuner System RNA Interference by shrna RNA Interference by microrna (mirna) Bacterial Expression Bacterial Expression and Purification Starter Kits Baculovirus Expression BacPAK Baculovirus Expression BacPAK qpcr Titration Kit, Media and Insect Cells Subtractive Hybridization Suppression Subtractive Hybridization (SSH), cdna Subtraction Kit PCR-Select Differential Screening Kit Matchmaker Gold Yeast Two-Hybrid Systems Matchmaker Gold Yeast One-Hybrid Systems Yeast Two Hybrid Libraries Inducible Protein-Protein Interactions idimerize Systems Co-Immunoprecipitation (Co-IP) Plasmid Isolation from Yeast Yeast Growth Media His-Tagged Protein Purification TALON - Cobalt-based resins His60Ni - Nickel-based resins GST-Tag Purification Resins Antibody Purification Resins DYKDDDDK-Tagged Protein Purification Anti-DYKDDDDK Beads Protease Inhibitor Cocktail

5 Unlock the Secrets of microrna mircury LNA Products for microrna Research 2012 Nucleus Cytoplasm? Argonaute P-bodies pri-mirna(s) Transcription RNA Pol II Micro processing Drosha DGCR8 (A)n pre-mirna Exportin-5 pre-mirna Dicing Dicer mirna duplex Asymmetric unwinding Dicer TRBP RISC loading RISC mature mirna mrna target selection Partial complementarity? v (A)n Argonaute (A)n Translation repression (A)n? RNA decay v (A)n mrna cleavage Near perfect complementarity

6 Isolation mircury TM RNA Isolation Kits Expression Analysis mircury LNA TM Universal RT microrna PCR System Expression Analysis mircury LNA TM microrna Array System Localization mircury LNA TM microrna Detection System of microrna Secrets the Unlock Functional Analysys for microrna Research 2012 mircury LNA Products mircury LNA TM microrna Inhibitors

7 Electrophorsis & Bioassay Products

8 DNA Transfection RNA Transfection Accessories Broad Spectrum Trans IT - LT1 Reagent Trans IT Reagent Trans IT - Express Reagent Cell Line Specific Trans IT Reagent Trans IT - 3T3 Reagent Trans IT - CHO Reagent Trans IT - COS Reagent Trans IT - HeLaMONSTER Reagent Trans IT - Jurkat Reagent Trans IT - Keratinocyte Reagent Trans IT - Neural Reagent Trans IT - Prostate Reagent cell line Protein Production Trans IT - PRO Kit 3D Transfection 3D Transfection System Trans IT - 3D Reagent sirna/mirna Trans IT-TKO Reagent Trans IT-siQUEST Reagent Trans IT-siPAK Kit Trans IT-siRAK Plus Kit mrna, Viral RNA Trans IT-mRNA Kit Oligo Transfection Trans IT-Oligo Kit DNA and RNA Electroporation Ingenio Electroporation Kits Ingenio Electroporation Solution Reporter Assay Beta-Gal Staining Kit Plasmid Cleanup MiraCLEAN Endotoxin Removal Kit Labeled Controls Label IT Plasmid Delivery Controls Cy3 Fluorescein Label IT RNAi Delivery Controls Cy3 Fluorescein Delivery Expression Vectors DNA Polymer-mediated Trans IT In Vivo Gene Delivery System Naked Nucleic Acid Trans IT -QR Starter Kit Trans IT -QR Delivery Solution Trans IT -EE Starter Kits Trans IT -EE Delivery Solution Gene Expression plive Vectors plive Vectors / LacZ Control Vector Kit plive Vectors / SEAP Control Vector Kit plive Vectors Complete System Cell Line Specific Polymer-mediated Trans IT In Vivo Gene Delivery System Naked Nucleic Acid Trans IT -QR Starter Kit Trans IT -QR Delivery Solution

9 Ribo-SPIA technology NuGEN s Portfolio of NGS Products..to enable simple, fast, and scalable NGS workflows

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