BioWhittaker Specialty Media Maximize Your Cell Growth and Productivity

Transcription

1 BioResearch BioWhittaker Specialty Media Maximize Your Cell Growth and Productivity BioWhittaker Specialty Media cell culture applications

2 BioResearch BioWhittaker Specialty Media Maximize Your Cell Growth and Productivity If your work leads to downstream therapeutic, diagnostic, or biopharmaceutical applications, serum-free media is the best choice initiating your research studies. BioWhittaker specialty media deliver outstanding permance, a broad selection, and consistent results, whether you need protein-free, non-animal (NAO) or chemically media. Our ISO 9000 and cgmp systems are recognized industry wide. BioWhittaker Serum-free Media deliver unique benefits: Consistent results Elimination of FBS lot qualification Elimination of FBS-borne mycoplasma and virus contamination Simplified downstream purification Reduced regulatory burdens Maximal yields General Purpose Media UltraCULTURE Serum-free Medium Delivers excellent growth and expansion of a broad range of adherent and suspension cells Simplify your media needs, no FBS needed The mulation UltraCULTURE has been submitted to the FDA as a product Master File PC-1 Defined, Serum-free Medium Superior media primary adherent cells HL-1 Defined, Serum-free Medium Unique media hybridoma and lymphocytic cells UltraCULTURE medium p Most cell types PC-1 CD medium p p Adherent primary cells and cell lines HL-1 CD medium p Hybridomas and many adherent cell lines Ordering inmation F UltraCULTURE medium 500 ml PC-1 CD medium 2 X 500 ml HL-1 CD medium 2 X 500 ml Mesenchymal Stem Cell Medium MSCGM-CD Defined, Serum-free MSC Growth Medium multiple passage expansion of all types of hmscs Cells can be directly transitioned from serum-containing medium with little to no adaptation time No need attachment matrix to plate cells Supports multi-lineage differentiation Ordering inmation Therapeak MSCGM-CD mesenchymal stem cell chemically growth medium BulletKit Kit Therapeak MSCBM-CD mesenchymal stem cell chemically basal medium 500 ml Therapeak MSCGM-CD mesenchymal stem cell chemically growth medium SingleQuots kit 5 ml 2

3 Fibroblast Medium FGM-CD Defined, Serum-free Fibroblast Growth Medium adult normal dermal fibroblasts and also supports neonatal cells Supports expression of fibroblast markers Ab5B5, CD90, and collagen I Collagen production is superior to that obtained with DMEM + FBS No weaning seamless transition from serum-containing media Ordering inmation FGM-CD fibroblast growth medium chemically, BulletKit with L-glutamine, without phenol red or antibiotics Kit FBM-CD fibroblast basal medium chemically 500 ml FGM-CD fibroblast growth medium chemically, SingleQuots kit Kit Keratinocyte Medium TheraPEAK KGM-CD Defined, Serum-free Keratinocyte growth medium Contains only non-animal orgin components KGM-CD medium supports both neonatal and adult keratinocytes Supports mation of an excellent barrier resulting in high TEER values Coating plates with fibronectin or other non-animal derived matrix material is necessary successful keratinocyte isolations with KGM-CD medium Ordering inmation CC-4455 TheraPEAK KGM-CD chemically medium 500 ml Cryopreservation Medium ProFreeze Defined Medium (2X), protein-free freezing medium Formulated cryopreserving all cell types Maintains high cell viability upon recovery from frozen storage Requires 15% DMSO at 2X Ordering inmation E ProFreeze chemically medium (2X) 100 ml 3

4 BioResearch BioWhittaker Specialty Media Hematopoietic Media X-VIVO Defined, Serum-free Hematopoietic Cell Media Superior media most hematopoietic cell types including dendritic cells, lymphocytes, monocytes, macrophages, and granulocytes Some mulations include recombinant transferrin to meet the highest regulatory requirements All current X-VIVO media products are manufactured under current GMP's and are listed with the FDA in a product Master File. Highly cited in scientific literature X-VIVO 10 medium p PBL, LAK, monocytes, macrophages, stem cells X-VIVO 15 medium p p PBL, NK, TIL, monocytes, macrophages, stem cells, dendritic cells X-VIVO 20 medium p PBL, LAK, TIL, monocytes, macrophages, stem cells, dendritic cells Ordering inmation Q X-VIVO 10 medium with L-glutamine, gentamycin and phenol red, with human transferrin 1 L Q X-VIVO 10 medium with L-glutamine, without gentamycin or phenol red, with human transferrin 1 L BE02-055Q X-VIVO 10 medium with L-glutamine, without gentamycin or phenol red, with recombinant transferrin 1 L BE04-418F* X-VIVO 15 medium with L-glutamine, gentamycin or phenol red, with human transferrin 500 ml Q X-VIVO 15 medium with L-glutamine, gentamycin or phenol red, with human transferrin 1 L BE02-053Q X-VIVO 15 medium with L-glutamine, gentamycin or phenol red, with recombinant transferrin 1 L Q X-VIVO 15 medium with L-glutamine, without gentamycin or phenol red, with human transferrin 1 L BE02-054Q X-VIVO 15 medium with L-glutamine, without gentamycin or phenol red, with recombinant transferrin 1 L Q X-VIVO 20 chemically, serum-free hematopoietic cell medium, with L-glutamine, gentamicin and phenol red 1 L * Europe only PER.C6 Medium ProPer 1 Defined, Serum-free Medium human embryonic retinoblast PER.C6 (and related) cells Recombinant protein and virus production Permexcis Defined, Serum-free Medium Maintain high viability (>90%) at high cell densities superior permance Save validation time as no weaning required during growth transition Reduce regulatory hurdles with a non-animal status ProPer 1 CD medium p p p PER.C6 (and related) cells in suspension Permexcis chemically (Please check the orgin and chemical boxes) Ordering inmation BE02-028Q ProPer 1 chemically medium 1 L BE02-039Q Permexcis virus production medium, without L-glutamine, phenol red or antibiotics 1 L 4

5 CHO Media PowerCHO Defined, Serum-free CHO Media New chemically PowerCHO media bring new levels of cell proliferation and protein production to chemically media Maintain high viability (>90%) at high cell densities UltraCHO Serum-free CHO Medium The al medium serum-free CHO use with suspension or adherent cells ProCHO CHO Media Multiple mulas to optimize your protein-free applications including adherent and suspension cells, with high proliferation rates and high protein yield Directly convert cultures from adherent with serum to suspension without serum PowerCHO and ProCHO IgG production ProCHO 4 supports concurrent transition to serum-free medium IgG (ng/ml) 2,500 2,000 1,500 1, Viable Cells /ml (x10 5 ) ProCHO 4 PowerCHO Passages PowerCHO 2 PowerCHO 3 ProCHO 4 Commercial supplier A Commercial supplier A Commercial supplier B Commercial supplier B Commercial supplier A + Insulin Commercial supplier C Commercial supplier D Commercial supplier B + Insulin CHO-K1 cells grown adherently in T-flasks using DMEM/F % FBS were subcultured directly into 500 ml spinner flasks containing 200 ml of the respective medium. PowerCHO 1, 2, 3 CD media p p p Suspension CHO ProCHO 4, 5 media p p Suspension CHO ProCHO AT medium p p Adherent CHO UltraCHO medium p Adherent and suspension CHO Ordering inmation Q PowerCHO 1 CD medium 1 L Q PowerCHO 2 CD medium 1 L Q PowerCHO 3 CD medium 1 L Q ProCHO 4 medium 1 L Q ProCHO 5 medium 1 L BE02-016Q ProCHO AT medium 1 L Q UltraCHO medium 1 L 5

6 BioResearch BioWhittaker Specialty Media Renal Media Pro293 Defined Media Formulas both suspension and adherent culture ProVero 1 Medium Supports Vero and MDCK in viral vaccine applications UltraMDCK Defined Medium Supports MDCK in viral vaccine applications at both high and low densities Pro293 s easy adaptation to suspension culture Pro293 a supports adherent high-density cell growth Viable Cells /ml (x 10 5 ) 12 60, , , ,000 Viable Cells (RFU) 4 20, , Days Pro293 s-cdm Commercial supplier A Pro293 a-cdm Commercial supplier B Days Pro293 s chemically medium p p p HEK 293 suspension Pro293 a chemically medium p p p HEK 293 adherent ProVero 1 medium p p Vero, MDCK UltraMDCK chemically medium p p MDCK, Vero Ordering inmation Q Pro293 s chemically medium 1 L Q Pro293 a chemically medium 1 L BE02-030Q ProVero 1 medium 1 L Q UltraMDCK chemically medium 1 L 6

7 Insect Medium Insect-XPRESS Insect Cell Medium Versatile medium shaker flask or stationary culture of SF9, SF21, High Five and Drosophila cells Insect-XPRESS medium p SF9, SF21, High Five and Drosophila cells Ordering inmation F Insect-XPRESS medium 500 ml Q Insect-XPRESS medium 1 L Hybridoma Media UltraDOMA Defined, Serum-free Hybridoma Medium Designed cultivation of murine, human and chimeric hybridomas in batch culture ProDoma Serum-free Hybridoma Medium, protein-free media your hybridoma needs Scalable from small flasks to large-scale bioreactors UltraDOMA -PF Defined, Hybridoma Medium Based on the al hybridoma media murine, human, and chimeric cells in a completely medium with no peptides or tissue extracts UltraDOMA chemically medium p p Murine, human, and chimeric cell lines UltraDOMA -PF chemically medium p p p p Murine, human, and chimeric cell lines ProDoma 1 chemically medium p p p Murine, rat, human, and chimeric cell lines ProDoma 3 chemically medium p p Murine, rat, human, and chimeric cell lines Ordering inmation B UltraDOMA chemically medium 500 ml F UltraDOMA -PF chemically medium 500 ml BE02-029Q ProDoma 1 chemically medium 1 L BE02-032Q ProDoma 3 chemically medium 1 L 7

8 Contact Inmation North America Customer Service: (toll free) Scientific Support: (toll free) Europe Customer Service: Scientific Support: International Contact your local Lonza distributor Customer Service: Online ordering International offices Australia Belgium Brazil France (toll free) Germany (toll free) India Ireland (toll free) Italy (toll free) Japan Luxemburg Poland Singapore Spain (toll free) The Netherlands (toll free) United Kingdom (toll free) Lonza Cologne GmbH Cologne, Germany For research use only. Not use in diagnostic procedures. PER.C6 and Permexcis are trademarks of Crucell N.V. High Five is a trademark of Boyce Thompson Institute Plant Research Inc. Unless otherwise noted, all trademarks herein are marks of the Lonza Group or its affiliates. The inmation contained herein is believed to be correct and corresponds to the latest state of scientific and technical knowledge. However, no warranty is made, either expressed or implied, regarding its accuracy or the results to be obtained from the use of such inmation and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. No statement is intended or should be construed as a recommendation to infringe any existing patent. Copyright 2011, Lonza Cologne GmbH. All rights reserved. BR-SpecMedia 01/12 CD-BR020