GenomeLab GeXP Genetic Analysis System

Transcription

1 GenomeLab GeXP Genetic Analysis System Octobe 2006 Beckman Coulte, Inc N. Habo Blvd., Fulleton, CA Copyight 2006 Beckman Coulte, Inc. Pinted in U.S.A.

2 Copyight, Licenses and Tademaks Copyight Beckman Coulte, Inc., All ights eseved. No pat of this publication may be epoduced, tanscibed, tansmitted, o tanslated into any language in any fom by any means without the witten pemission of Beckman Coulte, Inc. Licenses and Tademaks Beckman Coulte is a egisteed tademak of Beckman Coulte, Inc. GenomeLab is a tademak of Beckman Coulte, Inc. All othe tademaks ae popety of thei espective ownes.

3 Table of Contents Section 1 Oveview Geneal Desciption Section 2 Pe-Installation Pepaation Check List Section 3 Site Requiements Tabletop Space Section 4 Specifications GenomeLab GeXP System Specifications Section 5 Requied Consumable Mateials Sequence Analysis Fagment Analysis Section 6 Additional Mateials Mateials Requied but not Supplied Optional Mateials not Supplied by Beckman Coulte Section 7 GenomeLab GeXP System Taining Taining Plan iii

4 iv GenomeLab GeXP

5 Oveview Geneal Desciption 1 1Oveview 1.1 Geneal Desciption This guide povides the infomation necessay to pepae a laboatoy fo the installation of the GenomeLab GeXP Genetic Analysis System pio to its delivey to the laboatoy. It also povides infomation concening the post-installation taining of uses of the system. 1

6 Oveview Geneal Desciption 2 GenomeLab GeXP

7 Pe-Installation Pepaation Check List 2 2Pe-Installation 2.1 Pepaation Check List The following is a summaized list of the items that must be addessed pio to installation. Mak each item as it is completed. When these peequisites have been met, contact you local Beckman Coulte epesentative to schedule the installation. B Appopiate table o bench ("Tabletop Space" on page 5) B Suitable laboatoy envionment ("GenomeLab GeXP System Specifications" on page 7) B Suitable powe souce (Table 1 on page 7) B Consumable items (Table 3 on page 9 o Table 4 on page 10) B Custome supplied equipment and mateials (Table 5 on page 11) B Taining peequisites met ("Taining Plan" on page 13) Technical Suppot If you encounte a poblem that is not discussed in this guide and you need technical suppot, contact you local deale, the povide of this poduct, o contact Beckman Coulte diectly by using the infomation below: NOTE Wheneve you call you local deale o Beckman Coulte, be sue to have you egistation mateial, instument seial numbe, and softwae vesion numbe available. Fo futue efeence, ecod this infomation hee. Instument Seial Numbe: Softwae Vesion: Fimwae Vesion: Deale Name: Deale Phone Numbe: Mail Beckman Coulte, Inc Noth Habo Blvd. Fulleton, CA NOTE The system equies a netwok connection. Pio to aiving on site to install the instument, contact the site s IT depatment to aange fo netwok connections and vius potection. 3

8 Pe-Installation Pepaation Check List 4 GenomeLab GeXP

9 Site Requiements Tabletop Space 3 3Site Requiements 3.1 Tabletop Space The GenomeLab GeXP System is compised of the DNA analysis instument, an optional pinte and two (2) of the following: IBM PC towe monito keyboad mouse device Figue 1 shows the oveall system. Figue 2 shows the footpint and dimensions of the DNA analysis instument. Use this infomation along with the weight enty in Table 1 on page 7 to select an appopiate table o bench fo the system. CAUTION Ensue that the system is placed on a suface that is level and stong enough to suppot in excess of 200 lbs. Ventilation To povide adequate ventilation and convenient access to cabling, allow fo ten inches on the left side (facing instument) of the instument as well as at the ea of the IBM PC and the pinte. All othe sides of the equipment should have a six inch clea space to ensue pope ventilation (see Figue 2 fo the system dimensions). Figue 1 GenomeLab GeXP System Components 5

10 Site Requiements Tabletop Space Figue 2 System Footpint and Dimensions 361/2" 92.70cm 271/8" 68.90cm 261/4" cm 221/2" 57.15cm L.AI 6 GenomeLab GeXP

11 Specifications GenomeLab GeXP System Specifications 4 4Specifications 4.1 GenomeLab GeXP System Specifications Table 1 lists the specifications of the GenomeLab GeXP System. Use this infomation to pepae the envionment whee the system will be located. Table 1 System Specifications Envionment Item Weight (GenomeLab GeXP) Powe Requiements GenomeLab GeXP PC (typical) Monito (17 typical) Hub (typical) Fuses (GenomeLab GeXP) Maximum Heat Dissipation (to oom) GenomeLab GeXP PC and Monito Intenet Ambient Opeating Tempeatue Range Humidity Restictions Altitude Restictions PC (Towe) Installation Categoy Pollution Degee 2 Sound Pessue Level Indoo use only 81.8 Kg (180 lbs) Desciption CAUTION The supply voltage must not exceed 10% of nominal VAC, 5.0A, 50/60Hz 6.0A, 50/60Hz 1.8A, 50/60Hz 120VAC/60Hz and 220VAC/60Hz 6.3A Time Delay; 20mm (2 ea.); VAC 400W (1365 BTUs/Hou) 936W (3194 BTUs/Hou) All netwok connections via RJ-45 connecto pots and CAT 5 cables fo Local Aea Netwok (LAN) Ethenet with access to the Intenet C (59-86 F) <85% 30 C (86 F) up to 2000m (6,562ft) Beckman Coulte cetified contolle. Fo specific featues, contact you local Beckman Coulte epesentative. Categoy II Lase Categoy Class 1 Maximum Sound Pessue: 73 db Maximum Sound Pessue at 1 Mete away: 67 db WARNING This instument uses a Class 3B lase. The lase is housed in a sealed containe. Duing nomal opeation of the system, lase light is not accessible to the use. Theefoe, oveall lase classification of the sequence is Class 1. 7

12 Specifications GenomeLab GeXP System Specifications EMI Effect on GenomeLab GeXP Pefomance and Recommended Mitigations Unde the test conditions specified by the Euopean nomative electomagnetic compatibility standad EN , the GenomeLab instument may exhibit tempoay degadations in pefomance in accodance with Table 2 below. Because the envionmental cicumstances contibuting to the poblem can vay, seveal diffeent mitigation techniques have been povided that should help eliminate o educe the intefeence Table 2 EMI Effect on GenomeLab GeXP Pefomance and Recommended Mitigations. Test Condition Effect on Pefomance Mitigation Radio Fequency Field Intefeence (RFI) System exposed to electomagnetic field stengths of geate than o equal to 3 V / m at multiple fequency bands. May cause tempoay degadation of base calling accuacy. In some cases, significant noise will appea in the measued esults. Will etun to nomal pefomance once exposue is emoved. May cause tempoay loss of capillay tempeatue contol esulting in lowe than expected capillay tempeatue. Affect will be documented in the un log. Will etun to nomal pefomance once exposue is emoved. May cause tempoay loss of capillay tempeatue contol esulting in un not being initiated because tempeatue cannot each desied level. Re-un sample. Move location of the poduct by seveal metes. Change the oientation of the equipment by 90 degees. Avoid using tansmittes o cellula phones within 1 mete of the equipment. Review the tempeatues in the un log fo any anomalies and e-un as necessay. Apply mitigation techniques above. Disabling the tempeatue limit featue in the Method Edit sceen (Capillay Tempeatue box) will allow the un to be pefomed without desied tempeatue being eached. CAUTION The GenomeLab GeXP system equies five gounded electical eceptacles. The powe line to the laboatoy should be connected diectly fom a main powe line tansfome of a powe souce that is known to be clea of eatic powe loads, spikes and electomagnetic intefeence. CAUTION Powe lines used with this system must have an adequate eseve capacity to allow fo stat-up loads and the additional equipment of this system. Beckman Coulte ecommends that the nomal loading not exceed 50% of the nominal capacity of the powe lines. NOTE Installation of the GenomeLab GeXP PC contolle onto a local netwok is not equied fo the system to opeate. Installation, suppot and administation of local netwoks o intenet connections ae the esponsibility of the use. Netwok and intenet connections can expose the system to secuity isks, potential loss of data and possible system damage caused by hackes, softwae viuses o othe extenal factos. It is the uses esponsibility to ensue that appopiate secuity measues, anti-vius softwae, fiewalls and pocedues have been implemented. 8 GenomeLab GeXP

13 Requied Consumable Mateials Sequence Analysis 5 5Requied Consumable Mateials 5.1 Sequence Analysis Table 3 povides a list of the equied consumable items fo pefoming sequence analysis using the GenomeLab GeXP System. Contact you local Beckman Coulte epesentative fo specific consumable mateial equiements based on you application and fequency of use. Table 3 Requied Consumbables fo Pefoming Sequence Analysis Item P/N QTY Desciption Methods Development Kit (DNA Sequencing RXN Kit) o Dye Teminato Cycle Sequencing Kit fo 96 eactions. Includes: DNA polymease Dye Teminatos (ddutp, ddgtp, ddctp, ddatp) dntp Mix Solution Sequencing Reaction Buffe puc18 Contol Template -47 Sequencing Pime Glycogen Mineal Oil Sample Loading Solution (SLS) DTCS Quick Stat Kit Dye Teminato Cycle Sequencing Kit fo 96 eactions in a single tube containing: DNA polymease Dye Teminatos (ddutp, ddgtp, ddctp, ddatp) dntp Mix Solution Sequencing Reaction Buffe puc18 Contol Template -47 Sequencing Pime Glycogen Mineal Oil Sample Loading Solution (SLS) Sepaation Gel LPA ml of gel in CE system compatible containe. Sufficient to un two 96 well plates. Sepaation Buffe Each containe has a scew top and pou tip. The containe has enough buffe (30 ml) to fill a CE system 96-well, flat bottom Buffe Plate. (Each well being ¾ full.) 4/Pack DNA Sepaation Capillay Aay 33-75B Eight capillaies, 75 µm i.d., 33 cm long, 200 o.d. complete with electode block and detecto aay fitting. Ready fo installation into CE system. Buffe Plates Box Package of 100 flat-bottom polystyene plates, non steile, without lids. Requied fo use as the sepaation buffe plate. Sample Plates Box Package of 25 V-bottom themal cycle-compatible polypopylene plates with 200 µl volume capacity. Requied fo use as the CE system sample plate. 25 Plates/Box 9

14 Requied Consumable Mateials Fagment Analysis 5.2 Fagment Analysis Table 4 povides a list of the equied consumable items fo the pefoming fagment analysis using the GenomeLab GeXP System. Contact you local Beckman Coulte epesentative fo specific consumable mateial equiements based on you application and fequency of use. Table 4 Requied Consumables fo Pefoming Fagment Analysis Item P/N QTY Desciption DNA Sepaation Capillay Aay 33-75B Eight capillaies, 75 µm i.d., 33 cm long, 200 o.d. complete with electode block and detecto aay fitting. Ready fo installation into the GenomeLab GeXP. Sepaation Gel LPA ml of gel in CE system compatible containe. Sufficient to un two 96 well plates. Sepaation Buffe Each containe has a scew top and pou tip. The containe has enough buffe (30 ml) to fill a CE System 96-well, flat bottom Buffe Plate. (Each well being ¾ full.) 4/Pack DNA Size Standad Kit Contains fagments of 13 and 80 nucleotides designed to accommodate a wide ange of sizes fo multiplexed and poolplexed SNP fagments. DNA Size Standad Kit DNA size standad fo analysis of fagments up to 400 nucleotides. Includes: Mineal Oil DNA fagments of the following sizes labeled with CE system WellRED fluoescent dye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, and 420 nucleotides Sufficient fo 96 fagment analysis sepaations. DNA Size Standad Kit DNA size standad fo analysis of fagments up to 600 nucleotides. Includes: Mineal Oil SNPStat Kit Includes: Sample Loading Solution (SLS) DNA fagments of the following sizes labeled with CE system fluoescent dye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500, 520, 540, 560, 580, 600, 620, and 640 nucleotides Sufficient fo 96 fagment analysis sepaations SNPStat Maste Mix Contol Mineal oil Sample Loading Solution Sample Loading Solution (SLS), 6.0 ml Buffe Plates Box Package of 100 flat-bottom polystyene plates, non steile, without lids. Requied fo use as the CE system sepaation buffe plate. Sample Plates Box Package of 25 V-bottom themal cycle-compatible polypopylene plates with 200 µl volume capacity. Requied fo use as the CE system sample plate. 25 Plates/Box 10 GenomeLab GeXP

15 Additional Mateials Mateials Requied but not Supplied 6 6Additional Mateials 6.1 Mateials Requied but not Supplied Table 5 Additional Mateials Requied Item Sequence Analysis Fagment Analysis Refigeated micofuge Molecula Biology Gade: steile dh 2 O, 95% (v/v) ethanol/dh 2 O, 70% (v/v) ethanol/ dh 2 O Steile tubes ml micofuge, 0.2 ml thin wall themal cycling tubes o plates Themal cycle with heated lid Sample Loading Solution (SLS) Micoduste III AccTech, P/N: (VWR) Mini Alpha Swab fom Texwipe, PN: TX754B (VWR) 20 mg/ml Glycogen - Boehinge Mannheim Cat. # M Sodium Acetate ph5.2 - Sigma, Cat. # S mM Na 2 -EDTA ph8.0 (500 mm Sigma Cat. # 7889) PCR enzyme and buffe Labeled pimes (available fom Reseach Genetics) 6.2 Optional Mateials not Supplied by Beckman Coulte Aluminum Foil Seal and Sample, PN Themowell Cap Stips - Coning Cat. #

16 Additional Mateials Optional Mateials not Supplied by Beckman Coulte 12 GenomeLab GeXP

17 GenomeLab GeXP System Taining Taining Plan 7 7GenomeLab GeXP System Taining 7.1 Taining Plan Basic taining should take place immediately following the installation of the system. The Beckman Coulte Field Enginee will tain two uses in basic instument opeation, use of the softwae pogam and outine maintenance. Advanced taining sessions may be scheduled fo an additional fee. Contact you local Beckman Coulte epesentative fo moe infomation. To optimize the taining session, the following peequisites have been established fo uses: Expeience in the handling of biological samples Woking knowledge of the Windows XP Familiaity with DNA sequencing and/o fagment analysis NOTE Beckman Coulte Field Enginees do not povide Windows taining. 13

18 GenomeLab GeXP GenomeLab Genetic Analysis System (Octobe 2006) Copyight 2006 Beckman Coulte, Inc.. All ights eseved. No pat of this document may be epoduced o tansmitted in any fom o by any means, electonic, mechanical, photocopying, ecoding, o othewise, without pio witten pemission fom Beckman Coulte, Inc. Find us on the Wold Wide Web at: