HTS of Ca 2+ Transients in Human ips-derived Cardiomyocytes as a Predictive and Cost Effective Assay Early on in Drug Development
|
|
|
- Ashlee Singleton
- 6 years ago
- Views:
Transcription
1 Use our discoveries to advance yours HTS of Ca 2+ Transients in Human ips-derived Cardiomyocytes as a Predictive and Cost Effective Assay Early on in Drug Development Dr. Ralf Kettenhofen! Axiogenesis AG, Cologne, Germany Hamamatsu 10th European Functional Drug Screening Symposium! Actelion, Allschwil, Switzerland
2 Content Introduction into the production of Cor.4U cardiomyocytes Overview of established applications with Cor.4U cardiomyocytes HTS calcium transient assay in the Hamamatsu FDSS/ µcell Compound screening Data analysis Outlook 2
3 Generation of Cor.4U human ips derived Cardiomyocytes ips generated according to Yamanaka (licensed from ips Academia) Production system by Axiogenesis enables large lot sizes Tightly controlled differentiation results in minimal lot to lot variations Selection based purification technology results in pure cardiomyocytes Cryopreserved in various formats GFP positive and colourless varieties will be available Complete FTO for commercial use on the limited use label license Cryopreserved Fresh cultures on different substrates (cell culture flasks, 96/384 well plates) 3
4 4
5 Use our discoveries to advance yours Introduction Recording of Calcium Transients in Cor.4U Cardiomyocytes
6 Excitation-Contraction Coupling Action Potential Currents Na + Ca 2+ L-type Ca 2+ T-type Na + /Ca 2+ -exchanger K + Ito Amplitude K+ IKs K+ IKr K+ IKur Time Many Channel Types contribute to the Action Potential Calcium is the messenger that integrates the electrochemcial signals of the action potential with the molecular signaling pathways that regulate contraction
7 Hamamatsu FDSS/µCell
8 Plating Efficiency on 384 Well Plates Fluo-4 Assay Data was kindly provided by Dr. Thomas Licher, Sanofi Germany
9 FDSS Parameters Analysed by the CalDio/Wave Checker Software (3) P-P duration time (1)Peak Number (2)P rate(bpm) (6) A M P Bottom (7)Max_slope[Fluorescence counts/ms] same as upstroke slope (but bottom to peak) (8) MaxNeg_slope[Fluorescence counts/ms] same as downstroke slope (but peak to bottom) (5) RMP=Fluorescence intensity at the bottom peak AMP= Peak Fluorescence count- RMP (4)RATIO = AMP/RMP (9) APD 10 to 90 APD10 APD50(Peak Width, FWHM) M APD90 PWD 90 (1) Peak number (total, BPM) (2) P-P time [ms] (Ave, Std, Max, Min) (3) Ratio (Ave, Std)! Ratio = (AMP+RMP)/RMP (4) AMP (Ave, Std) (5) RMP (Ave, Std) (6) Slope (Ave, Std)! Rising Slope: Slope from bottom to peak! Falling Slope: Slope from peak to bottom! 0% - 10%, 10% - 90%, 20% - 80%, 30% - 70% (7) Integration (Ave, Std) (8) - (16) PWD (10% - 90%) [ms] (Ave, Std)
10 Use our discoveries to advance yours herg/ikr blocker
11 Astemizole herg Blocker Use our discoveries to advance yours
12 FDSS µcell - Astemizole 5 min vehicle control 41 nm 370 nm
13 FDSS µcell - Astemizole 30 min vehicle control 4.57 nm 13.7 nm
14 FDSS µcell - Astemizole 30 min 41 nm 123 nm 370 nm
15 Use our discoveries to advance yours Data Analysis with the Wave Checker Module Astemizole
16 FDSS µcell - Astemizole Wave Checker Module
17 FDSS µcell - Astemizole
18 FDSS µcell - Astemizole
19 FDSS µcell - Astemizole export of text file format
20 FDSS µcell - Astemizole
21 FDSS µcell - Astemizole
22 FDSS µcell - Astemizole
23 0,00# 5,00# 10,00# 15,00# 20,00# 25,00# 30,00# 35,00# 40,00# 45,00# 10000# 3333# 1111# 370# 123# 41# 14# 5# 2# 1# Control# bea$ng'frequency'[bpm]' concentra$on'[nm]' baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 35#min# 0,00# 2,00# 4,00# 6,00# 8,00# 10,00# 12,00# 14,00# 10000,00# 3333,33# 1111,11# 370,37# 123,46# 41,15# 13,72# 4,57# 1,52# 0,51# Control# average&falling&slope&[rfu/ms]& concentra7on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 35#min# FDSS µcell - Astemizole
24 0,00# 2000,00# 4000,00# 6000,00# 8000,00# 10000,00# 12000,00# 14000,00# 10000,00# 3333,33# 1111,11# 370,37# 123,46# 41,15# 13,72# 4,57# 1,52# 0,51# Control# average&peak&width&60%&[ms]& concentra8on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 35#min# 0,00# 2000,00# 4000,00# 6000,00# 8000,00# 10000,00# 12000,00# 14000,00# 10000,00# 3333,33# 1111,11# 370,37# 123,46# 41,15# 13,72# 4,57# 1,52# 0,51# Control# average&peak&width&70%&[ms]& concentra8on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 35#min# 0,00# 2000,00# 4000,00# 6000,00# 8000,00# 10000,00# 12000,00# 14000,00# 10000,00# 3333,33# 1111,11# 370,37# 123,46# 41,15# 13,72# 4,57# 1,52# 0,51# Control# average&peak&width&80%&[ms]& concentra8on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 35#min# 0,00# 2000,00# 4000,00# 6000,00# 8000,00# 10000,00# 12000,00# 14000,00# 16000,00# 18000,00# 10000,00# 3333,33# 1111,11# 370,37# 123,46# 41,15# 13,72# 4,57# 1,52# 0,51# Control# average&peak&width&90%&[ms]& concentra8on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 35#min# FDSS µcell - Astemizole
25 Dofetilide herg Blocker Use our discoveries to advance yours
26 FDSS µcell - Dofetilide 5 min vehicle control 24.7 nm 222 nm
27 FDSS µcell - Dofetilide 30 min vehicle control 24.7 nm
28 FDSS µcell - Dofetilide 0.00# 10.00# 20.00# 30.00# 40.00# 50.00# 60.00# 70.00# Control# 0.03# 0.10# 0.30# 0.91# 2.74# 8.23# 24.69# 74.07# # # # bea$ng'frequency'[bpm]' concentra$on'[nm]' baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 35#min# 0.00# 5.00# 10.00# 15.00# 20.00# 25.00# 30.00# 35.00# Control# 0.03# 0.10# 0.30# 0.91# 2.74# 8.23# 24.69# 74.07# # # # average&rising&slope&[rfu/ms]& concentra6on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 0.00# 1.00# 2.00# 3.00# 4.00# 5.00# 6.00# 7.00# 8.00# 9.00# 10.00# Control# 0.03# 0.10# 0.30# 0.91# 2.74# 8.23# 24.69# 74.07# # # # average&falling&slope&[rfu/ms]& concentra7on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min#
29 FDSS µcell - Dofetilide 0.00# # # # # # # # # Control# 0.03# 0.10# 0.30# 0.91# 2.74# 8.23# 24.69# 74.07# # # # average&peak&width&30%&[ms]& concentra8on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 0.00# # # # # # # # # Control# 0.03# 0.10# 0.30# 0.91# 2.74# 8.23# 24.69# 74.07# # # # average&peak&width&50%&[ms]& concentra8on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 0.00# # # # # # # # # # # Control# 0.03# 0.10# 0.30# 0.91# 2.74# 8.23# 24.69# 74.07# # # # average&peak&width&70%&[ms]& concentra8on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min# 0.00# # # # # # # # Control# 0.03# 0.10# 0.30# 0.91# 2.74# 8.23# 24.69# 74.07# # # # average&peak&width&90%&[ms]& concentra8on&[nm]& baseline# 5#min# 10#min# 15#min# 20#min# 25#min# 30#min#
30 FDSS µcell - Clustering of Compounds Confidential
31 Outlook
32 Outlook - Hamamatsu FDSS 7000EX Camera for fast recording of fluorescence and luminescence.
33 Outlook Evaluation of different kinds of fluorescent calcium and membrane potential dyes for the Cor.4U Cardiomyocytes Establishment of cardiac cytotoxicity/viability assay Combination of different luminescence assays viability cell death detection apoptosis
34 Dopaminergic and Sensory Neurons
35 Patch Clamp of hips-derived Dopamineric Neurons Manual current clamp recording of spontaneous action potentials Manual voltage clamp recording of sodium currents U[V] T[s] Data was kindly provided by PoreGenic GmbH, Rostock, Germany
36 Patch Clamp of hips-derived Peripheral Neurons Manual voltage clamp recording of sodium currents raw data I/V diagram 1.00E E E-009 I[A] -2.00E E E U[V] Data was kindly provided by PoreGenic GmbH, Rostock, Germany
37 Use our discoveries to advance yours Health and Environmental Science Institute (HESI) Initiative! CiPA Initiative (Comprehensive in vitro Pro-Arrhythmia Assay)
38
39 HESI - Initiative Stem Cell Working Groups - Newly Initiated Following the workshop, the Cardiac Stem Cell Working Group formed and three subteams were created: Cytotoxicity Contractility, Electrophysiology to explore issues of sensitivity, reproducibility, and predictivity for safety endpoints.
40 Cardiac Stem Cell Working Group: Timeline 4Q Q Q Subteam goals and objec1ves dra4ed Data collec1on ini1ated Literature search conducted Laboratory study protocol planning Laboratory study ini1ated Manuscript(s) dra4ing Manuscript(s) dra4ing and submission Link to CIPA project
41 Thanks to: Axiogenesis AG Anika Duenbostell Hamamatsu Jean Marc D'Angelo Thomas Niedereichholz Cyril Guerinot Thibault Poissinger Hirofumi Horai Sanofi, Frankfurt Dr. Thomas Licher
HTS automated patch clamp takes cardiac safety testing to the next level
Nanion Technologies HTS automated patch clamp takes cardiac safety testing to the next level Dr. Elena Dragicevic Senior Scientist / Sales Manager at Nanion Technologies Agenda Meet Nanion Technologies
Overview of FDSS application: With a focus on EFS assay
Overview of FDSS application: With a focus on EFS assay 9 th June 2016 Hamamatsu 12 th European Functional Drug Screening Symposium Natsumi KATO Application Engineer 1 Imaging Plate Reader FDSS series
TECHNOLOGY PLATFORMS FOR THE STUDY OF ION CHANNEL BIOLOGY AVAILABLE AT REACTION BIOLOGY CORP
TECHNOLOGY PLATFORMS FOR THE STUDY OF ION CHANNEL BIOLOGY AVAILABLE AT REACTION BIOLOGY CORP Introduction Ion channels are integral membrane proteins that mediate the regulated passage of charged particles
High Throughput (HTP) Fluorescence Imaging with Electric Field Stimulation (EFS): Phenotyping Human ipsc-derived Cardiomyocytes and Neurons
High Throughput (HTP) Fluorescence Imaging with Electric Field Stimulation (EFS): Phenotyping Human ipsc-derived Cardiomyocytes and Neurons Shouming DU, PhD. sdu@hamamatsu.com Oct 18, 2016 Content Part
CiPA Myocyte study update: Voltage-sensing optical (VSO) probes. Godfrey Smith University of Glasgow Clyde Biosciences UK
CiPA Myocyte study update: Voltage-sensing optical (VSO) probes Godfrey Smith University of Glasgow Clyde Biosciences UK VSOs provide a transmembrane potential signal from monolayers of spontaneously active
Myocyte Approaches to MEA & VSO Pilot Study & Preliminary Data
Myocyte Approaches to MEA & VSO Pilot Study & Preliminary Data Jiwen Zhang, Gary Gintant, Jennifer Pierson CiPA Myocyte Work Stream, Micro-Electrode Array (MEA) Subteam Voltage Sensitive Optical Probes
CIPA - Replacing the TQT with Non-clinical Proarrhythmia Testing: A New Paradigm
CIPA - Replacing the TQT with Non-clinical Proarrhythmia Testing: A New Paradigm Current status, opportunities, and challenges Hugo M. Vargas, PhD, DSP Scientific Director Safety & Exploratory Pharmacology
Triggering Calcium Responses in Various Human ipsc-derived Neural Cell Types. Giorgia Salvagiotto, PhD June 2016
Triggering Calcium Responses in Various Human ipsc-derived Neural Cell Types Giorgia Salvagiotto, PhD June 216 Transformative Potential of ipsc Technology: Enabling for Drug Discovery, Toxicology, and
Cor.4U on QPatch Cells provided by AxioGenesis
Application Report: Cor.4U on QPatch This report shows how cardiomyocytes from Axiogenesis can be used on the QPatch. Action potentials can be induced in a controlled and reproducible manner using a mix
Defining the CiPA Paradigm: An Update from the Ion Channel Working Group (ICWG) Bernard Fermini Pfizer Inc. Global Safety Pharmacology For the ICWG
Defining the CiPA Paradigm: An Update from the Ion Channel Working Group (ICWG) Bernard Fermini Pfizer Inc. Global Safety Pharmacology For the ICWG Ion Channel Working Group (ICWG) CiPA initiative is a
Manual patch clamp evaluation of herg channel 37 C and next steps
Manual patch clamp evaluation of herg channel pharmacology @ 37 C and next steps Wendy Wu, Ph.D. Division of Applied Regulatory Science Office of Clinical Pharmacology Office of Translational Sciences
Comprehensive In Vitro Proarrhythmia Assay Initiative (CiPA): Evolving Efforts
Comprehensive In Vitro Proarrhythmia Assay Initiative (CiPA): Evolving Efforts Gary Gintant for the CiPA Steering Committee & volunteers (CSRC/HESI/FDA/Japan Nat nl Inst. of Health Sciences, Health Canada,
herg Trafficking Assay -Luminescence
B SYS GmbH herg Trafficking Assay -Luminescence Specification Sheet B SYS GmbH herg Trafficking Assay - Luminescence Page 2 1. BACKGROUND 1.1. Drug-induced QT Prolongation The QT interval as measured in
Supplementary Information
1 Supplementary Information Supplementary Figures Supplementary Figure 1: The new CiPA concept and a role for OptoDyCE in cardiotoxicity testing. The new CiPA 1 (Comprehensive In Vitro Pro-arrhythmia Assay)
CSRC/HESI/SPS/FDA Think Tank. Ion Channel Testing Approach Najah Abi Gerges / Bernard Fermini December 11, 2014
CSRC/HESI/SPS/FDA Think Tank Ion Channel Testing Approach Najah Abi Gerges / Bernard Fermini December 11, 2014 Objectives of ICWG Address important questions related to best practices including: 1. Which
CHO-Tet herg Cell Line
F B SYS GmbH CHO-Tet herg Cell Line Specification Sheet B SYS GmbH B SYS GmbH CHO-Tet herg Cells Page 2 TABLE OF CONTENTS 1 BACKGROUND...3 1.1 Drug-induced QT Prolongation...3 1.2 Regulatory Issues...3
The Potential Role of CiPA on Drug Discovery, Development, and Regulatory Pathways
The Potential Role of CiPA on Drug Discovery, Development, and Regulatory Pathways David Strauss, MD, PhD Director, Division of Applied Regulatory Science Office of Clinical Pharmacology, Office of Translational
1.7 on Nanion's SyncroPatch 384PE. The electrophysiology team at Nanion Technologies GmbH, Munich, Germany. Cells were kindly provided by Anaxon.
Channel: h 1.7 Cells: CHO Tools: SyncroPatch 384PE Characterization of h 1.7 on Nanion's SyncroPatch 384PE The electrophysiology team at, Munich, Germany. Cells were kindly provided by Anaxon. Summary
SyncroPatch 384/768PE. Patch clamp meets HTS.
SyncroPatch 384/768PE. Patch clamp meets HTS. The SyncroPatch 384PE The SyncroPatch 768PE SyncroPatch 384/768PE. Patch clamp meets HTS. 384 768 cells in parallel (modular approach) Giga-seal recordings
Measuring ion channel block for CiPA:
Measuring ion channel block for CiPA: Lessons learned and recommendations Wendy W. Wu, Ph.D. Division of Applied Regulatory Science Office of Clinical Pharmacology, Office of Translational Sciences Center
SI8000 Live Cell Imaging System
SI8000 Live Cell Imaging System Sony Biotechnology Inc. SI8000 Cell Motion Imaging System The Sony SI8000 Live Cell Imaging System detects and quantifies cellular motion using proprietary video processing
CHO herg-duo Cell Line
B SYS GmbH CHO herg-duo Cell Line Specification Sheet B SYS GmbH B SYS GmbH CHO herg DUO Cells Page 2 TABLE OF CONTENTS 1. BACKGROUND...3 1.1. DRUG-INDUCED QT PROLONGATION...3 1.2. REGULATORY ISSUES...3
Rechanneling the Cardiac Proarrhythmia Safety Paradigm: An Update
Rechanneling the Cardiac Proarrhythmia Safety Paradigm: An Update Synopsis of the December 11 th, 2014 FDA/CSRC/HESI/SPS Think Tank Meeting Introduction and Background The year 2015 marks the 10 th anniversary
TECHNICAL BULLETIN. Cor.At GFP-i-Starter Kit. Catalog Number AXIO0059
Cor.At GFP-i-Starter Kit Catalog Number AXIO0059 TECHNICAL BULLETIN Product Description Cor.At cells are cardiomyocytes derived from transgenic mouse induced pluripotent stem (mips) cells. These cells
TECHNICAL BULLETIN. Cor.At GFP-i Cardiomyocytes (Mouse ips cell derived) Catalog Numbers AXIO0010 and AXIO0014
Cor.At GFP-i Cardiomyocytes (Mouse ips cell derived) Catalog Numbers AXIO0010 and AXIO0014 TECHNICAL BULLETIN Product Description Cor.At cells are cardiomyocytes derived from transgenic mouse induced pluripotent
FDSS Applications. Optogenetic. New High Resolution Camera. New Screening Technology. Cardiovascular Disease. Antibody.
Optogenetic Cardiovascular Disease New Screening Technology New High Resolution Camera Safety/Toxicology Antibody Cell Health and Energy Metabolism GPCR Enzymatic Assay High Speed Acquisition Protein-Protein
SUMMER SCHOOL LABORATORY ACTIVITIES
SUMMER SCHOOL LABORATORY ACTIVITIES ACTIVITIES Monday Tuesday Wednesday Thursday 18 th 19 th 20 th 21 st 1 and 2 A B C D 3 and 4 B C D A 5 and 6 C D A B 7 and 8 D A B C The students are divided into 4
Cor.At. Cardiomyocytes derived from Mouse Embryonic Stem Cells. Protocol. Cor.At Cardiomyocytes Transfection
Use our Discoveries to Help Advance Yours Cor.At Cardiomyocytes derived from Mouse Embryonic Stem Cells Protocol Cor.At Cardiomyocytes Transfection Using the Nucleofector Technology (Amaxa/Lonza Cologne)
CHO-hERG DUO optimized for QPatch
Application Report CHO-hERG DUO optimized for QPatch Functional validation and performance data for the CHO herg DUO cell line available from. This cell line is made in collaboration with B SYS GmbH (Switzerland).
Human ips/es Cell Technology and Its Applica;on to Toxicology Tes;ng
Human ips/es Cell Technology and Its Applica;on to Toxicology Tes;ng - Especially focusing on in vitro cardiac func;on toxicity- Atsushi Sanbuissho (Daiichi Sankyo Co. Ltd.) Kenji Yasuda (Tokyo Medical
Utility of preclinical PKPD modeling in QT safety testing
Utility of preclinical PKPD modeling in QT safety testing Sandra Visser & Piet van der Graaf EMA/EFPIA M&S Workshop on the role and scope of modelling and simulation in drug development BOS1, London 1
Stem Cell Services. Driving Innovation for Stem Cell Researchers
Driving Innovation for Stem Cell Researchers Stem Cell Services Partner with us and have access to the most advanced and comprehensive stem cell services available today. 675 W. Kendall St. Cambridge,
SOCIETY OF TOXICOLOGY Exhibitor-Hosted Sessions
SOCIETY OF TOXICOLOGY 2017 Baltimore, Maryland March 12-16, 2017 Exhibitor-Hosted Sessions Tuesday, March 14 10:30 AM to 11:30 AM CC Room 337 Exhibitor-Hosted Session: Neurotox and Cardiac Safety Assessment:
Recent years have witnessed an expansion in the disciplines encompassing drug
Course overview Recent years have witnessed an expansion in the disciplines encompassing drug discovery outside the pharmaceutical industry. This is most notable with a significant number of Universities
CiPA- Regulatory considerations/ Next steps K Prasad, MD, FRCP
CiPA- Regulatory considerations/ Next steps K Prasad, MD, FRCP Licensing Division, MHRA, Cardiovascular WP of EMA 1 Challenges/ Regulatory considerations 1. Construct of the CiPA package What and How different
Quantification of nuclear Ca 2+ transients in cardiac myocytes
ESC Congress 2010, Stockholm, 1 st September 2010 Quantification of nuclear transients in cardiac myocytes Senka Ljubojević a, Stefanie Walther a,b, Burkert Pieske a, Jens Kockskämper a, c Disclosures:
EarlyTox Cardiotoxicity Kit
EarlyTox Cardiotoxicity Kit Table 1-1: Available Kits Assay Kit Explorer Kit Bulk Kit EarlyTox Cardiotoxicity Kit R8210 R8211 The EarlyTox Cardiotoxicity Kit from Molecular Devices provides a fast, simple,
Development of Novel Advanced Cell Culture Surfaces that Provide Better Cell Growth and Attachment for Cell-Based Assays
Development of Novel Advanced Cell Culture Surfaces that Provide Better Cell Growth and Attachment for Cell-Based Assays Elizabeth J. Abraham, Ph.D. BD Biosciences Outline Overview of surfaces and culture
An Update on CiPA Activities from an FDA Perspective
An Update on CiPA Activities from an FDA Perspective Zhihua Li, Ph.D. Division of Applied Regulatory Science Office of Clinical Pharmacology, Office of Translational Sciences Center for Drug Evaluation
BioFarma USEF. Expertise in drug discovery projects
BioFarma USEF Expertise in drug discovery projects Since more than 15 years, we have provided custom assay development, compound profiling, high-throughput screening services and collaborations in over
SUMMER SCHOOL LABORATORY ACTIVITIES
SUMMER SCHOOL LABORATORY ACTIVITIES ACTIVITIES Monday Tuesday Wednesday Thursday 18 th 19 th 20 th 21 st 1 and 2 A B C D 3 and 4 B C D A 5 and 6 C D A B 7 and 8 D A B C The students are divided into 4
Introduction to Assay Development
Introduction to Assay Development A poorly designed assay can derail a drug discovery program before it gets off the ground. While traditional bench-top assays are suitable for basic research and target
Tips for Multiplexing Cell-Based Assays:
Tips for Multiplexing Cell-Based Assays: Plan for success Fall 2010 Click this icon to view speakers notes for each slide. 2010, Promega Corporation. Considerations for Successful Cell-Based Assays Assay
Cell Health and Viability Assays Real-time automated measurements of cell health and viability inside your incubator
INCUCYTE LIVE-CELL ANALYSIS SYSTEM Cell Health and Viability Assays Real-time automated measurements of cell health and viability inside your incubator See what your cells are doing and when they do it
Human ips/es Cell Technology and Its Application to Toxicology Testing
Human ips/es Cell Technology and Its Application to Toxicology Testing -Especially focusing on in vitro cardiac function toxicity- Atsushi Sanbuissho, PhD Daiichi Sankyo Co., Ltd. Kenji Yasuda, Prof Tokyo
Agilent Solutions for Measuring Cell Metabolism on your Plate Reader
gilent Solutions for Measuring Cell Metabolism on your Plate Reader Unlock the full potential of your plate reader Discover how gilent s MitoXpress Xtra oxygen consumption assay, ph Xtra glycolysis assay,
Peri.4U TM Application Protocol Multiwell-MEA
Peri.4U TM Application Protocol Multiwell-MEA Measuring Neuronal Electrical Activity of Peri.4U TM (Axiogenesis AG) on the 24-well Multiwell-MEA System (Multi Channel Systems MCS GmbH). Version 1.0: July
Accurate and Automated cell confluence assessment in microplates
Accurate and Automated cell confluence assessment in microplates TECAN S SPARK 20M MICROPLATE READER WITH INTEGRATED CELL IMAGING SIMPLIFIES CELL CULTURE QC AND SIGNAL NORMALIZATION TO CELL CONFLUENCE
Peri.4U. in Maestro microelectrode array (MEA) assays
Peri.4U in Maestro microelectrode array (MEA) assays User Guide Version 4.1 / March 2018 Contents 1. General Information 1 2. Safety Information 1 3. Material 1 3.1 Cells and media provided by Ncardia
GPCR induction followed by a fluorometric Ca 2+ assay in Thermo Scientific Varioskan LUX
GPCR induction followed by a fluorometric Ca 2+ assay in Thermo Scientific Varioskan LUX SP&A Application Laboratory, Thermo Fisher Scientific, Vantaa, Finland Application Notes Key Words Cellular signal,
Innovative Solutions for Drug Discovery and Life Sciences Research
Innovative Solutions for Drug Discovery and Life Sciences Research Unleash your brilliance Advancing cell and protein analysis technologies for your landmark scientific achievements Molecular Devices is
Cor.At. Cardiomyocytes derived from Mouse Embryonic Stem Cells. Technical Manual
Cor.At Cardiomyocytes derived from Mouse Embryonic Stem Cells Technical Manual Axiogenesis AG Nattermannallee 1, D-50829 Köln Tel.:+49-221-99 88 18-0, Fax: +49-221-99 88 18-10 Email: info@axiogenesis.com
Fluo-8 No Wash Calcium Assay Kit
ab112129 Fluo-8 No Wash Calcium Assay Kit Instructions for Use For detecting calcium in cells by using our proprietary fluorescence probe This product is for research use only and is not intended for diagnostic
Propelling GPCR drug discovery through innovative products and services Be the change the market seeks
Propelling GPCR drug discovery through innovative products and services Be the change the market seeks With the cost of bringing a new therapeutic agent to market now estimated at around $2 billion and
Application Note 2011/06. Orion II Microplate Luminometer Simplicity 4
Berthold Detection Systems GmbH Bleichstrasse 56 68 D-75173 Pforzheim/Germany Phone: +49(0)7231/9206-0 Fax: +49(0)7231/9206-50 E-Mail: contact@titertek-berthold.com Internet: www.titertek-berthold.com
CNS.4U Handling guide
CNS.4U Handling guide Human ipsc-derived central nervous system cells Last update: Oct. 2017 Europe Nattermannallee 1/S20 50829 Cologne, Germany +49 221 99 88 18-0 North America 600 W Germantown Pike,
Cor.4U in CardioExcyte 96 assays
Cor.4U in CardioExcyte 96 assays Application protocol Last update: July 2017 Europe Nattermannallee 1/S20 50829 Cologne, Germany +49 221 99 88 18-0 North America 600 W Germantown Pike, STE 110 Plymouth
Calcium Assay Kit. Technical Data Sheet. Product Information. Description. Storage. Materials not included
BD Technical Data Sheet Calcium Assay Kit Product Information Catalog Number: 640176 Size Reagents for 10 plates Components: Calcium Indicator, 1 vial, lyophilized 10X Signal Enhancer, 10 ml 1X Calcium
Fluo-8 Medium Removal Calcium Assay Kit
ab112128 Fluo-8 Medium Removal Calcium Assay Kit Instructions for Use For detecting calcium in cells by using our proprietary fluorescence probe This product is for research use only and is not intended
Why Doing Live-Cell Imaging?
Why Doing Live-Cell Imaging? The Snapshot Bias Different Endpoint = Different Result IncuCyte System Key Advantages IncuCyte Live-Cell Imaging System Software Advantage: Distributed Access NETWORK Unlimited
EarlyTox Cell Integrity Kit
EarlyTox Cell Integrity Kit The EarlyTox Cell Integrity Kit from Molecular Devices is an optimized set of reagents that simplifies the measurement of live and dead cells in a single well. The assay uses
CytoScan Fluoro Assay
089PR G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name CytoScan Fluoro Assay (Cat. # 786 211) think proteins! think G-Biosciences www.gbiosciences.com
Human Pluripotent Stem Cell Research for Regenerative Medicine and Drug Discovery
Human Pluripotent Stem Cell Research for Regenerative Medicine and Drug Discovery Our Multidisciplinary Academia-Industry Collaboration Project in Japan Norio Nakatsuji Professor and Founding Director
Rhod-4 No Wash Calcium Assay Kit
ab112157 Rhod-4 No Wash Calcium Assay Kit Instructions for Use For detecting calcium in cells without washing by using our proprietary fluorescence probe This product is for research use only and is not
CHO K V 4.3 Cell Line
B SYS GmbH CHO K V 4.3 Cell Line Specification Sheet B SYS GmbH B SYS GmbH CHO K V 4.3 Cells Page 2 TABLE OF CONTENTS 1. PRODUCT SHIPMENT...3 1.1. Product Format...3 1.2. Mycoplasma Certificate...3 1.3.
Cor.4U Cardiomyocytes. Human ipsc-derived Cardiomyocytes
Cor.4U Cardiomyocytes Human ipsc-derived Cardiomyocytes User Manual Updated January 2018 Contents 1. General Information 2 2. Safety Information 2 3. Material 3 3.1 Cells and media provided by Ncardia
PRODUCT DESCRIPTION Recombinant HEK293 cell line expressing the four subunits α1, β1, δ and ε of the human nicotinic acetylcholine receptor type I.
PRODUCT DATASHEET PrecisION hnachr α1/β1/δ/ε-hek Recombinant Cell Line Catalog Number: CYL3052 PRODUCT DESCRIPTION Recombinant HEK293 cell line expressing the four subunits α1, β1, δ and ε of the human
Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis
Introduction APPLICATION NOTE IncuCyte Live-Cell Analysis System Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis Susana L. Alcantara, Miniver Oliver, Kalpana Patel,
SensoLyte 520 Factor Xa Assay Kit *Fluorimetric*
SensoLyte 520 Factor Xa Assay Kit *Fluorimetric* Catalog # 72208 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit is optimized to detect Factor Xa enzyme activity Enhanced Value: It
Culture and Differentiation of Human Embryonic Stem Cells to Form Specific, Functional Cell Types. NAS June 3, 2010
Culture and Differentiation of Human Embryonic Stem Cells to Form Specific, Functional Cell Types NAS June 3, 2010 hescs: Specific Cell Types Available On Demand for Numerous Applications Human Embryonic
RECOMMENDED CULTURE CONDITIONS Recommended culture conditions and standard operating procedure are provided with the product.
PRODUCT DATASHEET PrecisION hnav1.5-hek Recombinant Cell Line Catalog Number: CYL3004 PRODUCT DESCRIPTION Recombinant HEK293 cell line expressing the human Nav1.5 (type V voltage-gated sodium channel alpha
A Comparison of PerkinElmer s LANCE camp Assay Performance to that of State of the Art Competitive Technologies
A Comparison of PerkinElmer s LANCE camp Assay Performance to that of State of the Art Competitive Technologies GPCR ASSAYS A P P L I C A T I O N N O T E Introduction G-protein coupled receptors (GPCRs)
Axol Bioscience SNAPSHOT PRESENTATION ELRIG ADVANCES IN CELL BASED SCREENING Zoe Allen, PhD Product Specialist
Axol Bioscience SNAPSHOT PRESENTATION ELRIG ADVANCES IN CELL BASED SCREENING 2017 Zoe Allen, PhD Product Specialist z.allen@axolbio.com Overview human cell culture systems induced pluripotent stem cells
An Automated, Cell-based Platform for the Rapid Detection of Novel Androgen Receptor Modulators
A p p l i c a t i o n N o t e An Automated, Cell-based Platform for the Rapid Detection of Novel Androgen Receptor Modulators Brad Larson and Peter Banks, BioTek Instruments, Inc., Winooski, VT Bruce Sherf,
Kinetic measurement of cytotoxicity using CellTox Green Cytotoxicity Assay on the IncuCyte TM FLR or ZOOM
Kinetic measurement of cytotoxicity using CellTox Green Cytotoxicity Assay on the IncuCyte TM FLR or ZOOM The CellPlayer TM cytotoxicity assay described in this protocol utilizes CellTox Green Dye (Promega,
Description. Lipodin-Pro TM - Protein Transfection Reagent. 1. Kit Benefits
Description Lipodin-Pro TM - Protein Transfection Reagent The delivery of proteins inside living cells represents an alternative to nucleic acids transfection and a powerful strategy for functional studies
CFSE Cell Division Assay Kit
CFSE Cell Division Assay Kit Catalog Number KA1302 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...
SensoLyte Rh110 Factor Xa Assay Kit *Fluorimetric*
SensoLyte Rh110 Factor Xa Assay Kit *Fluorimetric* Catalog # 72207 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit is optimized to detect Factor Xa enzyme activity Enhanced Value: It
Measurement of ion channel functions under in vitro conditions. Dr. Norbert Nagy Research Associate Department of Pharmacology and Pharmacotherapy
Measurement of ion channel functions under in vitro conditions Dr. Norbert Nagy Research Associate Department of Pharmacology and Pharmacotherapy Topics: -Electrophysiological techniques for basic research
IMAP TR-FRET Phosphodiesterase Evaluation Assay Kit With Progressive Binding System Quantity: 800 test points (20 L reactions)
Product Insert Product # R8176 IMAP TR-FRET Phosphodiesterase Evaluation Assay Kit With Progressive Binding System Quantity: 800 test points (20 L reactions) The IMAP TR-FRET Phosphodiesterase Assay Kit
NEW INSIGHTS. NEW DISCOVERIES. Real-time automated measurements of cell health, movement and function inside your incubator.
THE NEXT GENERATION HAS ARRIVED IncuCyte S3 Live-Cell Analysis System Real-time automated measurements of cell health, movement and function inside your incubator. NEW INSIGHTS. NEW DISCOVERIES. See what
Integration of FDSS7000 into a modular robotic system for Open Innovation drug discovery
Integration of FDSS7000 into a modular robotic system for Open Innovation drug discovery José Manuel Brea & María Isabel Loza BioFarma, Santiago de Compostela DRUG DISCOVERY OPEN INNOVATION CLOSED innovation
Measurement by Spark 20M / Spark 10M Multimode microplate reader
Application Note Fluorospark Kinase/ADP Multi-Assay Kit Measurement by Spark 20M / Spark 10M Multimode microplate reader Introduction Fluorospark Kinase/ADP Multi-Assay Kit Phosphorylation and dephosphorylation
FLIPR Potassium Assay Kit
FLIPR Potassium Assay Kit Table A-1: Available Kits Assay Kit Explorer Kit Bulk Kit FLIPR Potassium Assay Kit R8222 R8223 The FLIPR Potassium Assay Kit from Molecular Devices provides a fast, simple, and
BD Ratiometric Calcium Assay Kit
BD Technical Data Sheet BD Ratiometric Calcium Assay Kit Product Information Catalog Number: 644243 Components: Ratiometric Calcium Indicator, 1 vial, lyophilized 10X Signal Enhancer, 10 ml Calcium Assay
Transcreener PDE Assay
Technical Manual Transcreener PDE Assay Transcreener PDE Assay Kit Catalog #3006-1K, 3006-10K 1.0 Introduction p.2 2.0 Assay Principle p.2 3.0 Instrumentation p.3 4.0 Transcreener PDE Assay Kit Components
NEW INSIGHTS. NEW DISCOVERIES. Real-time automated measurements of cell health, movement and function inside your incubator.
THE NEXT GENERATION HAS ARRIVED IncuCyte S3 Live-Cell Analysis System Real-time automated measurements of cell health, movement and function inside your incubator. NEW INSIGHTS. NEW DISCOVERIES. See what
ApoStrand ELISA Apoptosis Detection Kit
Enabling Discovery in Life Science ApoStrand ELISA Apoptosis Detection Kit Instruction Manual BML-AK120 For research use only BML-AK120-page 1 - ApoStrand ELISA Apoptosis Detection Kit BML-AK120 BACKGROUND
Cor.4U in microelectrode array (MEA) assays
Cor.4U in microelectrode array (MEA) assays Application protocol Last update: Oct. 2017 Europe Nattermannallee 1/S20 50829 Cologne, Germany +49 221 99 88 18-0 North America 600 W Germantown Pike, STE 110
LightCycler 480 Instrument Quick Guide Protein Melting
LightCycler 480 Instrument Quick Guide Protein Melting Protein Melting Analysis Principle: Protein Melting is monitored using hydrophobic binding dyes, like SYPRO Orange, and is used to determine the stability
Mathematical modelling of the action potential of human embryonic stem cell derived cardiomyocytes
Paci et al. BioMedical Engineering OnLine 2012, 11:61 RESEARCH Open Access Mathematical modelling of the action potential of human embryonic stem cell derived cardiomyocytes Michelangelo Paci 1, Laura
Ratiometric Calcium Assay Kit
BD Technical Data Sheet Ratiometric Calcium Assay Kit Product Information Catalog Number: 644244 Components: Ratiometric Calcium Indicator, 10 vials, lyophilized 10X Signal Enhancer, 100 ml Description
SensoLyte Homogeneous AFC Caspase-8 Assay Kit *Fluorimetric*
SensoLyte Homogeneous AFC Caspase-8 Assay Kit *Fluorimetric* Catalog # 7288-2 Kit Size 2 Assays (96-well plate) Optimized Performance: This kit is optimized to detect caspase-8 activity. Enhanced Value:
Application Note. ADME and cytotoxicity assays Use of the CyBi -Well for reagent addition and washing procedures
ADME and cytotoxicity assays Use of the CyBi -Well for reagent addition and washing procedures Heidi Prüfer, Michael Busch; CyBio Screening GmbH, Jena Cells have several strategies which they can use to
Accelerating Scientific Discovery
Elite TM Fluorescent Membrane Potential Dye Kit CATALOG NUMBER: CA-M165 Description camp is a key second messenger involved extensively in cellular signal transduction pathways associated with the majority
Supplementary Figures
Supplementary Figures Supplementary Figure S1. In vitro determination of the detection limit of vanco-800cw (a) Specificity of vanco-800cw for different clinical bacterial isolates. The in vitro imaging
FLUORESCENCE TRACKING OF CARDIAC MYOCYTE EXCITATION
FLUORESCENCE TRACKING OF CARDIAC MYOCYTE EXCITATION Phys 173 / BGGN 266 Amy Davenport and Laurent Palmatier 06/12/09 ABSTRACT Cultured cardiac myocytes were fluorescently tagged with fluo-4am and stimulated
CHO Na V 1.4 Cell Line
B SYS GmbH CHO Na V 1.4 Cell Line Specification Sheet B SYS GmbH B SYS GmbH CHO Na V 1.4 Cells Page 2 TABLE OF CONTENTS 1. B'SYS Na V 1.4 CELL LINE...2 1.1 THE VOLTAGE GATED SODIUM CHANNELS NA V 1.4...2
Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, MD 21702, USA
Title: Cross-Site Reliability of Human Induced Pluripotent Stem-Cell Derived Cardiomyocyte Based Safety Assays using Microelectrode Arrays: Results from a Blinded CiPA Pilot Study Running Title: CiPA hsc-cm
MEA Application Note: Primary Culture Cardiac Myocytes from Chicken Embryo
MEA Application Note: Primary Culture Cardiac Myocytes from Chicken Embryo Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without