In Situ Bioremediation Technologies by Tersus Environmental
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1 In Situ Bioremediation Technologies by Tersus Environmental
2 Page 2 Gas infusion
3 Page 3 Tersus Environmental In Situ bioremediation Gas delivery Edible oil Achieve site closure Low cost solution Soil vapor extraction
4 Page 4 Groundwater Bioremediation Category Aerobic Anaerobic Cometabolic Technology Gas infusion Oxygen Sparge Systems Peroxygens Gas infusion Electron Donors Gas infusion Example Target Contaminants Petroleum Hydrocarbons Chlorinated Solvents Dioxane, NDMA, Chloroform, PCE, TCE, MTBE, atrazine
5 Page 5 Soil Remediation MicroBlower Remote soil vapor extraction system U. S. Patent 6,971,820
6 Page 6 Gas infusion isoc Technology Microporous Hollow Fiber Mass transfer device Supersaturates treatment well without sparging Bioremediation stimulated by delivery of substrates isoc
7 Page 7 Complete isoc System Two Stage Low Flow Regulator set at 50psi Distribution Header, set for 1 isoc Reusable Filter w. quick disconnect Nitrogen Bubble Release Every 1-7 Seconds Oxygen Supply, 1cf Usage Per Day Polyurethane Tubing Down Well Flood Resistant isoc Unit in 2 Well
8 Page 8 Working Demo isoc System
9 Page 9 isoc Area of Influence isoc Gas Infusion Air Sparging
10 Page 10 Dissolved Gas Concentrations Gas Type Water Column Depth in Feet (Dissolved Gas in ppm) Oxygen Methane Propane Hydrogen Ethane Carbon Dioxide 1,660 1,875 2,090 2,300 3,590
11 Page 11 isoc Well Schematic Gas infusion Well Water Table Regulator and Manifold Gas Supply Filter Contaminated Groundwater Treatment Zone Groundwater Flow isoc Unit
12 Page 12 Installation Photos
13 Page 13 Ground Vault Installation
14 Page 14 Injection Well Concentration Gradient Technology Dissolved Oxygen (ppm) isoc Peroxygens 3 Oxygen Emitters Air Sparging 8-11
15 Page 15 Gas infusion Technologies inventures isoc HiSOC BioFlo gpro CO 2 SWI NAPL Recovery
16 Cometabolic Bioremediation
17 Page 17 Cometabolism Fortuitous degradation Bugs derive no energy Contaminants are not carbon source Focus Dioxane NDMA Chlorinated compounds
18 Page 18 Degradation Pathways Aerobic Cometabolic TCE TCE epoxide CO 2, CL DCE DCE epoxide CO 2, CL VC VC epoxide CO 2, CL Anaerobic Reductive Dechlorination PCE TCE DCEs VC ETH
19 Page 19 Cometabolic Bioremediation Benefits Reduces amendment costs Applicable to low contaminant concentrations Achieve site closure
20 Page 20 Gas Management System Oxygen Gas Supply Gas infusion Wells Alkane Gas Supply Nitrogen Connection Groundwater Flow Filter Contaminated Groundwater Treatment Zone isoc Unit
21 Chlorinated Solvent Site Case Study
22 Page 22 Former Industrial Facility
23 Page 23 System Design Two isoc units Industrial grade oxygen and propane Housed separately in portable garden sheds
24 Page 24 cis-1,2-dichloroethylene
25 Page 25 Chlorobenzene
26 Page 26 1,2 Dichloroethane
27 Page 27 Chlorobenzene
28 Page 28 Results DO increased 35 feet north and 50 feet west DAP successfully supplied depleted nutrients Effectively distributed dissolved propane
29 Page 29 Conclusions Reduced COCs Allowing approval of MNA and monitoring Gained closure for groundwater
30 Vinyl Chloride Case Study
31 Page 31 Vinyl Chloride Case Study Old gravel pit site PCE release stalled at VC Plume 3000 x 400 x 30 Maximum VC: 30 ppb Anoxic groundwater High iron & sulfate (Mapped Plume 2002 > 3000 feet long)
32 Page 32 Proof of Concept Microcosm O 2, ethene and nutrients 1 um methane = 16 ppb; 1 um ethene = 28 ppb; 1 um VC = 62.5 ppb
33
34 RB-58 isoc Biobarrier with 20 Treatment Wells (under construction) isoc Treatment Well Line HST-2 34
35 Page 35 Performance Data (mid-plume) +
36 Page 36 Performance Data (downgradient)
37 EDS (Electron Donor Solution) Advancing the Science of In Situ Groundwater Remediation
38 Page 38 Target Contaminants Chlorinated organics Oxidized metals Energetic materials Nitrate Radionuclides Acid rock drainage
39 Page 39 Self Emulsifying Oil Movie Clip
40 Page 40 Hydrogen is What Counts Soybean Oil Based Electron Donor Pounds of Hydrogen per Pound of Product Equivalent Pounds for a Pound of EDS-ER EDS-ER (93% soybean oil) EVO (45% soybean oil) EVO (50% soybean oil EVO (55% soybean oil) EVO (60% soybean oil)
41 Page 41 Soluble Amendment EDS-QR Quick release >98% natural seed oils No Water
42 Page 42 Water Soluble Oil Movie Clip
43 Page 43 Convergence of Gas infusion Technology & Electron Donors Hydrogen-enriched water reduces demand for electron donor Saves money
44 Supersaturated Water Injection for Enhanced NAPL Recovery in Source Zones
45 Page 45 NAPL Source Zone Challenges Trapped free-phase LNAPL Air channeling / fingering Poor access of injected air to residual NAPL Slide courtesy of Marios A. Ioannidis
46 Page 46 Supersaturated Water Injection Water is supersaturated with CO 2 Supersaturated water is then injected into the aquifer CO 2 bubbles nucleate in the aquifer Water In Supersaturated Water Out Unsaturated Zone SWI Well Extraction Well Saturated Zone Exsolution zone
47 Page 47 Conceptual Model Rising CO 2 bubbles Contact hydrocarbons Cause volatilization Groundwater and soil vapor are extracted Water In Supersaturated Water Out Unsaturated Zone SWI Well Extraction Well Saturated Zone Exsolution zone
48 Page 48 Proof of Concept in the Lab In Situ Gas Saturation Development and Rate of Gas Ebullition Bubble flow meter V g1 V g2 V g3 Water outlet and level control V w Injection Saturated porous medium Production Supersaturated water, C
49 Page 49 In Situ Gas Evolution SWI
50 Page 50 Recovery of Residual Hexane
51 Page 51 Recovery of Residual Hexane
52 Page 52 Modeling of Lab Experiments 125 cm 125 cm Inlet 45 cm Outlet Inlet Boundary 45 cm Outlet Boundary Experimental Apparatus FEMLAB Simulation Domain
53 Page 53 Comparison of Simulation to Experiment kl 0.01S g s min 0 min min min v in = cm/s, C 0 = 5.44 g/l
54 Page 54 Proof of Concept in the Field Enclosed cell at CFB Borden, Ontario 200 L of hydrocarbon mixture added to saturated zone Pentane Hexane Soltrol Nelson. Field Trial of Residual LNAPL Recovery Using CO2- Supersaturated Water Injection in the Borden Aquifer, MS Thesis, University of Waterloo, 2007
55 Page 55 The Test Cell Nelson. Field Trial of Residual LNAPL Recovery Using CO2- Supersaturated Water Injection in the Borden Aquifer, MS Thesis, University of Waterloo, 2007
56 Page 56 NAPL Mass Removal Pentane Hexane Total Mass (kg) % Mass (kg) % Mass (kg) % Phase I Phase II Total Nelson. Field Trial of Residual LNAPL Recovery Using CO2- Supersaturated Water Injection in the Borden Aquifer, MS Thesis, University of Waterloo, 2007
57 Page 57 Demonstration Conclusions Recovery by volatilization alone 77 % of the Pentane 53 % of the Hexane Majority of the NAPL mobilized towards water table Available for liquid phase recovery
58 Page 58 Injection Photos gpro Gas infusion System Carbonated Water Sample Injection Well
59 Page 59 CO2 Injection Movie Clip
60 Page 60 CO 2 SWI Benefits Significant improvement Greater: Zone of influence Recovery rate Percentage removed Trapped NAPL mobilized upwards by gas bubbles Cost effective
61 MicroBlower Remote Soil Vapor Extraction U. S. Patent 6,971,820
62 Page 62 MicroBlower Solar powered soil vapor extraction Enhanced barometric pumping
63 Page 63 MicroBlower Installations
64 Page 64 MicroBlower Data Savannah River Site
65 Page 65 Soil Gas VOC Concentrations
66 Page 66 VOCs Removed 234 pounds removed in 10 months
67 Page 67 Micro Blower Movie Clip
68 Page 68 TersOX Inorganic Peroxygen for Enhanced Aerobic Bioremediation TersOx is proprietary formulation of food-grade, calcium hydroxide that produces a controlled-release of molecular oxygen. TersOx stimulates natural degradation of petroleum hydrocarbons such as benzene, toluene, ethylbenzene and xylenes (BTEX). This is not a chemical oxidation product. The high ratio of O 2 in TersOx (>16% by weight) provides a long-term oxygen source for up to 12 months upon hydration. This sustained release of oxygen stimulates indigenous bacteria, accelerates bioactivity, and promotes increased contaminant removal.
69 Page 69 TersOX TersOx Specifications A white to yellow, powdery material Calcium peroxide Releases >16% of its weight as oxygen when hydrated Packaged and delivered in 110 lb fiber drums Expected shelf-life of material - 2 years *MSDS for full product specifications available upon request
70 Page 70 TersOX Field Applications TersOx and water mixture (slurry) application in excavations Dry powder application in soils and excavations Injectable slurry for source area and permeable reactive barrier applications
71 Page 71 TersOX Benefits Controlled-release of molecular oxygen to support aerobic microbial biodegradation Long-term source of oxygen to the subsurface Clean, low-cost, non-disruptive application No Operations and Maintenance
72 Page 72 Contact Information Craig E. Marlow Cemcor Environmental Services 8248 Hidden Forest Drive, Holland, Ohio Office: Cell: CES Web Page:
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