Associazione Italiana Allevatori Laboratorio Standard Latte

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2 The (LSL) of Italian Breeders Association (AIA) was born in 1990 thanks to the contribute of the Ministry of Agriculture (MIPAAF). The main mission was to coordinate the activity of the 25 AIA-LSL laboratories. The analytical coordination cover an important role because the analytical results produced are an important factor and they are used for DHI improvements. The main activities of LSL are the production of reference materials and the organization of proficiencies test. About 200 laboratories of the Italian dairy sector, participate in the proficiency tests and use reference materials (industries, private laboratories, public institutes for animal health and universities). The LSL is certified CSQA according the ISO 9001:2008 for Design and Production of certified reference materials for dairy sector, design, organization and carrying out of Proficiency Test and it is accreditated Accredia_SINAL department (N.138). The LSL s staff is member of national and international organization of standardization as ISO,IDF and UNI. This participation is important to refer in these committees the daily problems and analytical difficulties met by the LSL s clients. All these observations are collected by the LSL staff with an active and proactive listening. 2

3 The LSL s staff is very well educated and motivated to orient the customers in the right choose of our reference materials and proficiency test to control and improve own analytical accuracy. To facilitate the plan activities of our customers we provide an annual calendar with our supplying. We sincerely hope that this catalogue could be an useful tool to check your analytical processes. 3

4 REFERENCE MATERIALS INTRODUCTION The use of Reference Materials is an important tool to verify the accuracy long the time, to validate a new method or the modifications on a standard method. If it is used in a system of quality assurance is an objective tool that allows the quality of analytical results according the ISO IEC DESCRIPTION The provides Reference Materials according ISO Guide 34:2000 General Requirements for the competence of reference material producers e ILAC G12:2000 Guidelines for the Requirements for the Competence of Reference Materials Producers. The batch homogeneity is checked according the ISO 13528:2005 Statistical methods for use in Proficiency Testing by Interlaboratory Comparisons. The assigned value is obtained with the execution of reference method (if it is not differently specified) and the specific proficiency test. The high number of participants and an accurate statistical elaboration assure that the characterization procedure is independent and objective. The objectivity of reference materials is also due to the confront with others European providers. In the certificate of Reference Materials is reported the parameter s value, 4

5 its uncertainty (expressed as standard deviation of reproducibility). USE Each Reference Material is specific and it must be used according the specification reported in the technical sheet and the instruction for its use. The stability and homogeneity of Reference Materials is assured till the expire date reported if the sample is maintained and preserved according the instructions. The Reference Materials if are opened should be used as soon as possible. The provider doesn t assure the homogeneity and stability if the sample is splitted in sub-samples DATA COMMUNICATION The certificate of the reference material is sent by and published on the web site: 5

6 FAT, PROTEIN, CASEIN, LACTOSE, UREA COW MILK FT 001 (series of 10 samples) FT 002 (series of 5 samples) Sample description: 80 ml raw cow milk sample in a screw colored cap plastic vials. Scope: to be used as reference material to control or calibrate Mid-Infrared instruments. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,02 % (wt/vol) and Sodium Azide 0,02 % (wt/vol). Shelf life: 9 days since its production. 6

7 FAT, PROTEIN, LACTOSE, UREA BUFFALO MILK FT 003 Sample description: series of 6 raw buffalo milk sample in a 80 ml screw colored cap plastic vials. Scope: to be used as reference material to control or calibrate Mid-Infrared instruments. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,02 % (wt/vol) and Sodium Azide 0,02 % ( wt/vol ). Shelf life: 9 days since its production. 7

8 FAT, PROTEIN, CASEIN, LACTOSE, UREA SHEEP MILK FT 004 Sample description: series of 6 raw sheep milk sample in a 80 ml screw colored cap plastic vials. Identification: the samples are identified by a label with Scope: to be used as reference material to control or calibrate Mid-Infrared instruments. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,02 % (wt/vol) and Sodium Azide 0,02 % (wt/vol). Safety: this product is supplied for laboratory use only. Shelf life: 9 days since its production. 8

9 FAT, PROTEIN, LACTOSE, UREA GOAT MILK FT 005 Sample description: series of 6 raw goat milk sample in a 80 ml screw colored cap plastic vials. Scope: to be used as reference material to control or calibrate Mid-Infrared instruments. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,02 % (wt/vol) and Sodium Azide 0,02 % (wt/vol). Shelf life: 9 days since its production. 9

10 FAT, PROTEIN, LACTOSE WHEY FT 509 Sample description: series of 5 frozen whey sample in a 80 ml screw colored cap plastic vials. production, an alphanumeric identification and product code. Scope: to be used as reference material to control or calibrate Mid-Infrared instruments. Instruction for storage and use: keep the sample frozen until its use. Best if used shortly after opening. Preservative: Bronopol 0,03 % (wt/vol). Shelf life: 2 years since its production. 10

11 SOMATIC CELLS FT 006 Sample description: series of 20 cow milk samples, including 5 different cells level ( cells/ml), 4 vials for each level. Samples are in a 24 ml screw black cap glass vials. Scope: to be used as reference material to control or calibrate fluoro-optic-electronic somatic cell counters. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,03 % (wt/vol). Shelf life: 4 months since its production. 11

12 SOMATIC CELLS PILOT SAMPLE FT 041A Sample description: 24 ml cow milk sample, heat treated, at a cells count of approximately cells/ml. The sample is in a 24 ml screw black cap glass vials. Scope: to be used as reference material to control or calibrate fluoro-optic-electronic somatic cell counters. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,03 % (wt/vol). Shelf life: 4 months since its production. 12

13 SOMATIC CELLS PILOT SAMPLE FT 041B Sample description: 24 ml cow milk sample, heat treated, at a cells count of approximately cells/ml. The sample is in a 24 ml screw black cap glass vials. Scope: to be used as reference material to control or calibrate fluoro-optic-electronic somatic cell counters. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,03 % (wt/vol). Shelf life: 4 months since its production. 13

14 DEOXYNIVALENOL (DON) CORN FLOUR FT 477 Sample description: 50 g ± 0,1 g corn flour samples under vacuum packed. Scope: to be used as reference material for DON determination in corn flour by ELISA and HPLC. Instruction for storage and use: store at 4 C ± 2 C lightproof. Best if used shortly after opening. Shelf life: 1 year since its production. 14

15 AFLATOXIN B1 CORN FLOUR FT 409 (series of 4 samples) FT 410 (single sample) Sample description: 25 g ± 0,1 g corn flour samples under vacuum packed. Scope: to be used as reference material for Aflatoxin B1 determination in corn flour by ELISA and HPLC. Instruction for storage and use: store at 4 C ± 2 C lightproof. Best if used shortly after opening. Shelf life: 1 year since its production. 15

16 FUMONISIN CORN FLOUR FT 511 Sample description: series of two 25 g ± 0,1 g corn flour samples under vacuum packed. Scope: to be used as reference material for Fumonisin determination in corn flour by ELISA and HPLC. Instruction for storage and use: store at 4 C ± 2 C lightproof. Best if used shortly after opening. Shelf life: 1 year since its production. 16

17 ZEARALENON CORN FLOUR FT 478 Sample description: series of two 50 g ± 0,1 g corn flour samples under vacuum packed. Scope: to be used as reference material for Zearelon determination in corn flour by ELISA and HPLC. Instruction for storage and use: store at 4 C ± 2 C lightproof. Best if used shortly after opening. Shelf life: 1 year since its production. 17

18 OCRATOXIN A GRINDED GRAIN FT 479 Sample description: series of three 50 g ± 0,1 g granted grain samples under vacuum packed. Scope: to be used as reference material for Ocratoxin A determination in grain by ELISA and HPLC. Instruction for storage and use: store at 4 C ± 2 C lightproof. Best if used shortly after opening. Shelf life: 1 year since its production. 18

19 AFLATOXIN M1 COW MILK FT 395 (series of 4 samples) FT 391E (single sample) Sample description: 10 ml lyophilized skim milk sample in an amber glass vials with rubber cup and metal seal. Scope: to be used as reference material for Aflatoxin M1 determination in milk by ELISA. Instruction for storage and use: store at < 0 C until its use. Best if used shortly after opening. Preservative: Bronopol (0,03% wt/vol). Shelf life: 1 year since its production. 19

20 AFLATOXIN M1 COW MILK FT 391H Sample description: 80 ml frozen skim milk sample in a screw colored cap plastic vials. Scope: to be used as reference material for Aflatoxin M1 determination in milk by HPLC. Instruction for storage and use: keep the sample frozen until its use. Best if used shortly after opening. Preservative: Bronopol (0,03% wt/vol). Shelf life: 6 months since its production. 20

21 CRYOSCOPY WATER SOLUTION FT 210 Sample s descriptions: series of 3 water solution sample at different freezing point (-0,500-0,530 C). Samples are in a 9 ml amber glass vials with rubber cup and metal seal. Scope: to be used as reference material for thermistor or plateau cryoscope. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Shelf life: 1 year since its production. 21

22 CRYOSCOPY PILOT SAMPLE FT 480 Sample description: 24 ml cow milk sample, heat treated, at a freezing point of approximately 0,520 C series. The sample is in a 24 ml screw black cap glass vials. Scope: to be used as reference material for thermistor or plateau cryoscope. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Shelf life: 1 year since its production. 22

23 ANTIBACTERIAL SUBSTANCES IN MILK NEGATIVE CONTROL FT 308A Sample description: 10 ml lyophilized skim milk sample in an amber glass vials with rubber cup and metal seal. Scope: to be used as negative control in test for detection of antibacterial substances in milk. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Shelf life: 2 years since its production. 23

24 ANTIBACTERIAL SUBSTANCES IN MILK POSITIVE CONTROL PENICILLIN G MRL FT 308B Sample description: 10 ml lyophilized skim milk sample in an amber glass vials with rubber cup and metal seal. Scope: to be used as positive control in test for the detection of antibacterial substances in milk. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Shelf life: 2 years since its production. 24

25 ANTIBACTERIAL SUBSTANCES IN MILK POSITIVE CONTROL SULFAMIDIC MRL FT 308D Sample description: 10 ml lyophilized skim milk sample in an amber glass vials with rubber cup and metal seal. Scope: to be used as positive control in test for the detection of antibacterial substances in milk. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Shelf life: 2 years since its production. 25

26 KJELDAHL FT 037 Sample description: 1,5 g lyophilized skim milk sample in an amber glass vials with rubber cup and metal seal. Scope: to be used as reference material for the determination of total nitrogen content by Kjeldahl Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Shelf life: 2 years since its production. 26

27 TOTAL BACTERIA COUNT FT 206 Sample descriptions: series of 4 lyophilized skim milk with a bacteria count ranging from a cfu/ml. Samples are in a screw cap sterile plastic vials. Scope: to be used as reference material to control and calibrate fluoro-optic-electronic counters. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Sodium Azide 0,02 % (wt/vol). Shelf life: 1 year since its production. 27

28 TOTAL BACTERIA COUNT PILOT SAMPLE FT 208 Sample description: 24 ml lyophilized skim milk with a bacteria count ranging from a cfu/ml. The sample is in a screw cap sterile plastic vials. Scope: to be used as reference material to control and calibrate fluoro-optic-electronic counters. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Sodium Azide 0,02 % (wt/vol). Shelf life: 1 year since its production. 28

29 UREA STANDARD 1 FT 110A Sample description: 4 ml skim milk sample, heat treated, at a urea content of about 30 mg/100 ml. The sample is in a 4 ml screw black cap glass vials. Scope: to be used as reference material to calibrate differential ph, conductometric and colorimetric instruments. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,03 % (wt/vol). Shelf life: 6 months since its production. 29

30 UREA STANDARD 0 FT 110B Sample description: 4 ml skim milk sample, heat and urease treated, at an urea content less than 3 mg/100 ml. The sample is in a screw black cap glass vials. Scope: to be used as reference material to calibrate differential ph, conductometric and colorimetric instruments. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,03 % (wt/vol). Shelf life: 6 months since its production. 30

31 UREA FT 223 Sample description: series of 4 heat treated milk sample, at an urea content ranging from 10 to 80 mg/100ml. The sample is in a 24 ml screw black cap glass vials. Scope: to be used as reference material to control or calibrate Mid-Infrared instruments. Instruction for storage and use: store at 4 C ± 2 C. Best if used shortly after opening. Preservative: Bronopol 0,03 % (wt/vol). Shelf life: 6 months since its production. 31

32 PROFICIENCY TEST INTRODUCTION Taking part in the Proficiency Test you can evaluate your accuracy and stability long time term. If it is used in a context of quality assurance it is an objective tool to check the reliability of analytical results as requested by ISO IEC Description The Proficiency Test provided by AIA-LSL are planned and provided according ISO Accuracy (trueness and precision) of measurement methods and results -- Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method, ILAC G13:08/2007 Guidelines for Requirements or the Competence of Providers of Proficiency Testing Scheme. According these International Standard AIA-LSL assures that all data are maintained anonymous. Each Proficiency Test has enclosed with a detailed protocol with the sample description matrix, indication on analyses execution and how to send back the results. 32

33 USE The participation to the Proficiency Test allows to validate and to verify alternative method, because it is possible to identify the systematic error (trueness) or random (repeatability and reproducibility) of your analyses. The Proficiency Test is use to characterize the reference materials as well. The results obtained in the trials it is important to plan the corrective actions to maintain stable the analytical process of the laboratory. DATA COMMUNICATION The report of the Proficiency Test will be send by to the participants and separately each participant will receive the own code of identification. The report will be published on the web site: The download is allowed to the clients that previously require Username and Password to AIA-LSL. 33

34 ROUTINE METHODS COW MILK FT 012 Cow milk samples to determine fat, Protein, Casein, Lactose, Somatic Cells, antibacterial substances, Cryoscopy, ph, Urea, dry matter, Titrable Acidity. ROUTINE METHODS SHEEP MILK FT 307 Sheep milk samples to determine Fat, Protein, Lactose, Cryoscopy. ROUTINE METHODS BUFFALO MILK FT 476 Buffalo milk samples to determine Fat, Protein, Lactose, Cryoscopy. ROUTINE METHODS GOAT MILK FT 563 Goat milk samples to determine Fat, Protein, Lactose, Cryoscopy. REFERENCE METHODS FT 019 Cow milk to determine Fat and Protein by reference methods: Rose Gottlieb e Kjeldahl. CELLULE SOMATICHE FT 217 Cow milk samples to determine somatic cells by using fluoro-opto-electronic counters. 34

35 CHEESE FT 469 Cheese samples to determine Fat, Protein, Moisture, Ash, ph, and NaCl UREA FT 218A Cow milk for the determination of Urea by differential ph, colorimetric and conductometric methods. UREA FT 218B Cow milk for the determination of Urea by Mid-Infrared Instruments. CRYOSCOPY FT 216 Cow milk samples to determine the freezing point by thermistor instruments. TOTAL BACTERIA COUNT FT 215 Cow milk samples to determine the total bacteria count by using fluoro-opto-electronic counters. 30 C FT 309 Cow milk samples to determine aerobic bacteria according to the ISO 4833:

36 AFLATOXIN M1 FT 333E Series of cow milk samples to determine Aflatoxin M1 by ELISA. AFLATOXIN M1 FT 333H Series of cow milk samples to determine Aflatoxin M1 by HPLC. AFLATOXIN M1 FT 334 Two series of cow milk samples to determine Aflatoxin M1 by ELISA and HPLC. AFLATOXIN B1 FT 341 Series of corn flour samples to determine Aflatoxin B1 by ELISA or HPLC. AFLATOXIN B1 FT 342 Two series of corn flour samples to determine Aflatoxin B1 by ELISA and HPLC. DEOXYNIVALENOL (DON) FT 470 Series of corn flour samples to determine DON by ELISA or HPLC. DEOXYNIVALENOL (DON) FT 472 Two series of corn flour samples to determine DON by ELISA and HPLC. 36

37 ZEARALENON FT 473 Series of corn flour samples to determine Zearalenon by ELISA or HPLC. ZEARALENON FT 475 Two series of corn flour samples to determine Zearalenon by ELISA and HPLC. FUMONISIN FT 510 Series of corn flour samples to determine Fumonisin by ELISA or HPLC. FUMONISIN FT 512 Two series of corn flour samples to determine Fumonisin by ELISA and HPLC. OCHRATOXIN A FT 425 Series of granted grain samples to determine Ochratoxin A by ELISA or HPLC. OCHRATOXIN A FT 427 Two series of granted grain samples to determine Ochratoxin A by ELISA and HPLC. 37