Real-time PCR Product Selection Guide

Size: px

Start display at page:

Download "Real-time PCR Product Selection Guide"

Franklin Cannon
6 years ago
Views:

1 Real-time PCR Product Selection Guide Taqman Probe SYBR Green RealMOD Green AP 5x RealMOD Probe HP 5x Cat.No / 250 rxn. / Cat.No / 250 rxn. / RECOMMEND RealMOD Green W² 2x Real-time PCR RECOMMEND RealMOD Probe W² 2x Cat.No / / Cat.No / / RealMOD Green Fast (LR/S) RealMOD LS Cat.No / 5 / S Cat.No / 5 / Cat.No / 1 / Cat.No / 5 / RECOMMEND RealMOD Probe W² 2x qrt-pcr mix Cat.No / / RealMOD Green qrt-pcr mix (LR/S) LS Cat.No / / S Cat.No / / Real-time RT-PCR RealMOD Probe HiSenScript qrt-pcr mix Cat.No / 50 rxn. / 02

2 Real-time PCR performance is possible regardless of a ROX dye requirement (5X type) RealMOD Green AP 5X Cat.No / 250 rxn. / + High specificity and reproducibility + Excellent efficiency in case of using low copy number targets + Superior performance with long (up to 500bp) and + Very low signal-to-noise ratio by suppressing GC-rich templates primer-dimer formation RealMOD Green AP 5x is designed to use regardless of a ROX dye requirement and enable to do standard or fast qpcr. Compatible with below instruments SYBR Green Real-time PCR can be performed easily (2X type) RealMOD Green W² 2x Cat.No / / + High specificity and reproducibility + Excellent efficiency in case of using low copy number targets + Very low signal-to-noise ratio by suppressing + Excellent qpcr performance with GC-rich templates primer-dimer formation RealMOD Green W² 2x is designed to do standard qpcr. Compatible with below instruments 03

Much faster qpcr master mix than general Real-time PCR products RealMOD Green FAST (LR) Cat. No.

25454 / 5 / (LR/S) + Fast activation time within 30sec + Denaturation time within 1sec + Possible for annealing/extension within 10sec + Fast quantification possible for various target genes +

Green FAST using cdna and gdna template Quantitative analyses for samples were performed by diluting by one-fifth of 20ng of cdna and gdna template.

999 RealMOD Green Fast Supplier A Fig. 1. Detection of Human GAPDH expression with RealMOD Green Fast Fig. 2.

3 Much faster qpcr master mix than general Real-time PCR products RealMOD Green FAST (LR) Cat. No / 5 / (S) Cat. No / 5 / (LR/S) + Fast activation time within 30sec + Denaturation time within 1sec + Possible for annealing/extension within 10sec + Fast quantification possible for various target genes + Minimizing the risk of external contamination using + Able to select products according to Real-time PCR instrument 2X master mix type Technical Data + Higher reproducibility and efficiency RealMOD Green FAST using cdna and gdna template Quantitative analyses for samples were performed by diluting by one-fifth of 20ng of cdna and gdna template. Ct value in amplification plot appears constantly for all samples. In addition, Signal-to-noise of NC in the experiment using fluorescent dye was also merely detected RealMOD Green Fast Supplier A Fig. 1. Detection of Human GAPDH expression with RealMOD Green Fast Fig. 2. Comparison of RealMOD Green Fast qpcr Instrument : Applied Biosystems StepOnePlus Real-Time PCR System, Target : Human GAPDH, Template : HEK293 cdna and gdna mix with supplier A (20ng, 4ng, 800pg, 160pg, 32pg, 6.4pg and 1.3pg) / NC One-step type qrt-pcr mix from cdna synthesis to Real-time PCR RealMOD Green (LR) Cat. No / / (S) Cat. No / / qrt-pcr mix (LR/S) + High efficiency with Hot-start DNA polymerase + High sensitivity and very low signal-to-noise ratio + Easy-to-use provided by premix type + Higher reproducibility and efficiency + Possible with small amount of RNA + Able to select products according to Real-time PCR instrument Technical Data Results of sensitivity and elimination of non-specific amplification using RNA template Real-time RT-PCR performed with RealMOD Green qrt-pcr mix according to RNA sensitivity. In result, amplification plots formed amplification curves at a regular interval down to 10pg of RNA. Fig. 1. Detection of Human GAPDH expression with RealMOD Green qrt-pcr mix Instrument : Applied Biosystems 7500 Real-Time PCR System, Target : Human GAPDH, Template : HEK293 RNA (100ng, 10ng, 1ng, 100pg, and 10pg), Target assay method : SYBR Green 04 intron A Company B Company Fig. 2. Melt curves of the corresponding intron, A company, and B company

4 Real-time PCR performance is possible regardless of a ROX dye requirement (5X probe type) RealMOD Probe HP 5X Cat.No / 250 rxn. / + High specificity and reproducibility in gdna and cdna + Able to control Real-time PCR reaction volume + 5X master mix type able to singleplex or duplex qpcr + Excellent qpcr performance with AT-rich or GC-rich templates RealMOD Probe HP 5x is designed to use regardless of a ROX dye requirement and enable to do standard or fast qpcr. Compatible with below instruments Able to do standard Real-time PCR performance easily using Taqman probe (2X type) RealMOD Probe W² 2x Cat.No / / + High specificity and reproducibility + Excellent efficiency in case of using low copy number targets + Very low signal-to-noise ratio by suppressing + Excellent qpcr performance with GC-rich templates primer-dimer formation RealMOD Probe W² 2x is designed to do standard qpcr. Compatible with below instruments 05

5 2X master mix type Real-time PCR product based on gene specific primer and probe RealMOD Probe Cat. No / 1 / Cat. No / 5 / + Ready-to-use without any requirements + Able to control Real-time PCR reaction volume + High sensitivity able to quantify templates less than pg + Wide dynamic range + Able to detect gene accurately in a variety of templates Technical Data Quantification analysis of Real-time PCR using various target genes Quantification analyses were performed with various target genes using RealMOD Probe. The results from the various specimens have shown that the products is suitable for quantitative and qualitative analysis. A B C D E F G H (A), van A gene(bacterial gdna); (B), van B gene(bacterial gdna); (C), RNase P(human gdna); (D), M gene of Influenza A(cDNA); (E), van C1 gene(bacterial gdna); (F), NA gene of Influenza A(cDNA); (G), HA gene of InfA H1(cDNA); (H), NP gene of Swine influenza virus (cdna) Able to do standard Real-time PCR performance easily using Taqman probe (2X type) RealMOD Probe W² 2x Cat.No / / qrt-pcr mix + High specificity and reproducibility + Excellent efficiency in case of using low copy number targets + Very low signal-to-noise ratio by suppressing + Excellent qpcr performance with GC-rich templates primer-dimer formation RealMOD Probe W² 2x qrt-pcr mix is designed to do standard qpcr. Compatible with below instruments 06

6 One-step RT-PCR product for 5' nuclease assay Real-time quantitative PCR analysis RealMOD Probe Cat. No / 50 rxn. / HiSenScript qrt-pcr mix + Performing cdna synthesis and PCR in a tube at + One-step RT-PCR product with high specificity of antibody-based Hot-start DNA polymerase a time with RNA template + PCR amplification with high sensitivity synthesizing even a trace of + Stable reproducibility and an ease of use to be provided for quantitative RT-PCR experiment RNA to cdna in RT reaction at 45 for 30mins + Showing better activation in the absence of RNase H activation decomposing RNA/DNA hybrid Technical Data Possible for Multiplex quantitative RT-PCR RealMOD Probe HisenScript qrt-pcr mix is able to amplify at maximum four target RNAs with high sensitivity and to show high specificity to any RNA molecules. HisenScript RH(-) RTase lacking RNase H activity and i-startaq GH DNA polymerase featuring antibody-based Hot-start function make stable and the best results from one-step quantitative RT-PCR with high sensitivity and specificity. 3plex Target A Target B Target C Fig. 1. Analysis amplification in triplex RT-PCR and singleplex RT-PCR with RealMOD Probe HiSenScript qrt-pcr mix. Triplex, real-time One-step qrt-pcr was performed on the ABI 7500 real-time PCR system using the RealMOD Probe HiSenScript qrt-pcr mix and TaqMan probes. The template was 10 fold diluted total RNA from the Dengue virus type 2 and 3. Reactions were performed in triplicate. Target A (Dengue type 2) was detected using a JOE labeled probe. Target B (Dengue type 3) was detected using a FAM labeled probe. Target C (control) was detected using a Cy5 labeled probe. Real-time PCR/RT-PCR Products Product Name Real-time PCR Real-time RT-PCR Cat. No. Capacity Price RealMOD Green AP 5x rxn. RealMOD Green W² 2x RealMOD Green Fast (LR) RealMOD Green Fast RealMOD Probe HP 5x rxn. RealMOD Probe W² 2x RealMOD Probe RealMOD Green qrt-pcr mix (LR) RealMOD Green qrt-pcr mix RealMOD Probe HiSenScript qrt-pcr mix rxn. RealMOD Probe W² 2x qrt-pcr mix

7 intron Biotechnology, Inc. #605, 5th JoongAng Induspia, 137, Sagimakgol-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do, Korea Tel: Fax:

8 Product Selection Guide for Real-time PCR

Technical Review. Real time PCR

Technical Review. Real time PCR Technical Review Real time PCR Normal PCR: Analyze with agarose gel Normal PCR vs Real time PCR Real-time PCR, also known as quantitative PCR (qpcr) or kinetic PCR Key feature: Used to amplify and simultaneously