Achieving High Performance With Rapid Resolution HT (1.8um) Columns

Transcription

1 Achieving High Performance With Rapid Resolution HT (1.8um) Columns Columns and Supplies Solutions Group Toll Free Telephone Number for US/Canada: International Telephone Number: Toll Free International Numbers: Belgium Norway Denmark Spain France Sweden Germany Switzerland Italy UK Netherlands Robyn C. Woolley Online Application Engineer June 14, 2006

2 Achieving High Performance With Rapid Resolution HT (1.8um) Columns Columns and Supplies Solutions Group Toll Free Telephone Number for US/Canada: International Telephone Number: Toll Free International Numbers: Belgium Norway Denmark Spain France Sweden Germany Switzerland Italy UK Netherlands Robyn C. Woolley Online Application Engineer June 14, 2006 Starts in One Minute

3 Presenter: Robyn Woolley Robyn is an Online Technical Support and Applications Engineer for LC columns and consumables. She has been with Agilent for three years, and has over ten years experience in the pharmaceutical industry -- specifically in analytical R&D GMP/GLP regulated environments. Robyn currently assists customers with questions and problems on HPLC columns as well as identifying relevant applications for laboratory investigations. Slide 3

4 What is High Performance with Rapid Resolution High Throughput (RRHT) 1.8um Columns? High Resolution better resolution than with 3.5 and 5um particle sizes High Efficiency higher efficiency than with 3.5 and 5um particle sizes High Peak Capacity better resolution of complex mixtures, another measure of efficiency Options for Fast and Ultra-Fast and High Resolution Analysis Consistent Performance with different dimensions, at different pressures, and with 3.5 and 5um particle sizes ( scales easily) Optimum resolution with multiple bonded phases (selectivities) Long Lifetime Slide 4

5 How Do We Achieve High Performance with 1.8μm (Rapid Resolution High Throughput) Columns 1. Pick a strong particle to withstand higher pressures for routine and high pressure operation 2. Bond it with multiple familiar bonded phases select the right one for the separation and all particle sizes 1.8, 3.5 and 5um give the same performance 3. Pack it into columns specially designed for high performance with small particles at higher pressures a) Short columns for fast and ultra-fast LC, long columns for high resolution b) ID s from mm for the widest range of applications 4. Verify performance use performance measures outlined (efficiency, resolution, scalability, lifetime) 5. Use it routinely a) In new 1200 Series RRLC at pressures up to 600 bar and temperatures up to 100 C b) In older LC s capable of 400 bar (limited configurations) Slide 5

6 1. Pick a Strong Particle for High Pressure Use - Compression Test Comparison Compressure Pressure (psi) Compression Distance (in) Waters Aquity 1.7 µm Rx80, 1.8 µm Rx80 SB-C18, 5 µm Typical Daiso-100-5P Xerogel, 5μm ZORBAX Rx-Sil is very strong and is a good 1.8um choice. Other silicas are not as strong. Slide 6

7 2. ZORBAX RRHT Bonded Phase Choices Multiple Selectivities for Best Separation ZORBAX Eclipse XDB first choice for method development, general purpose bonded phase, for most applications, use up to 60 C ZORBAX StableBond good for low ph and high temperature applications, for those who want to raise temperature for faster analysis SB-C18 use up to 100 C and down to ph <1 (high temp only at low ph) SB-C8 use up to 80 C and down to ph 1 SB-CN use up to 80 C at ph 2 ZORBAX Extend-C18 good at high ph up to ph 11.5 at ambient temperature Slide 7

8 Multiple Bonded Phase Choices are Important for Best Separations Improve selectivity by changing the stationary phase. mau SB-C8 α=1.35 Rs= 6.3 mau Eclipse XDB-C8α=1.14 Rs= mau Eclipse XDB-C8 α=1.58 Rs= 10.0 mau SB-C8 α= 1.20 Rs= Mobile phase: (63:37) water :acetonitrile ml H 3 PO 4 Flow = 2.0 ml/. isocratic Temp. : ambient Sample: children's ibuprofen oral suspension, with benzophenone as internal std. prepared as described in USP Mobile phase: (70:30) 200mM NaH 3 PO 4 ph 2.5 :MeOH Flow =1 ml/. isocratic Temp. : 50 C LC: Agilent 1100 w. thermally controlled column compartment Sample: doxepin HCl in mp 0.1 mg/ml (82% E-isomer) Columns: ZORBAX RRHT, 4.6 x 50 mm 1.8 μm Slide 8

9 Optimum Selectivity Resolution and Analysis Time Should be Considered (SB Family) Columns: RRHT 4.6 x 50 mm 1.8um Mobile Phase A: ph M Na Acetate, B: ACN (70:30) Flow Rate: 1 ml/ Inj. Vol: 2 ul Detection: UV 219 nm Indole Pindolol RRHT SB-C18, 1.8um RRHT Eclipse XDB-C18, 1.8um RRHT SB-Phenyl, (exp) 1.8um RRHT SB-CN, 1.8um Slide 9

10 Different Bonded Phases Can be Used to Optimize a Separation Eclipse and SB Sample: Xanthines 1-methylxanthine Column: Eclipse XDB-C18 α 2,3 = µm 1,3-dimethyluric acid 3,7-dimethylxanthine 1,7-dimethylxanthine Column: SB-C µm α 3,2 = Column: SB-CN 1, µm Column: 4.6 x 50mm, 1.8um as noted Mobile Phase: A= 0.1% formic, B=0.1% formic in MeCN Eclipse XDB-C18 (95:5), SB-C18: 98:2 Flow Rate=1mL/ Inj. Vol: 6, 4, 2 ul Detection: UV, 254 nm Flowcell: 5uL, 6 mm flow path Slide 10

11 Selectivity is Constant as a Function of Particle Size Eclipse XDB-C18 Columns 5 µm α 2,3 = 1.13 Sample: Xanthines 1-methylxanthine 1,3-dimethyluric acid 3,7-dimethylxanthine 1,7-dimethylxanthine α 2,3 = µm α 2,3 = µm Column: 4.6 x 50mm Eclipse XDB-C18 Mobile Phase: A= 0.1% formic, B=0.1% formic in MeCN (95:5) Flow Rate=1mL/ Inj. Vol: 6, 4, 2 ul Detection: UV, 254 nm Flowcell: 5uL, 6 mm flow path Slide 11

12 No Difference in Column Selectivity as a Function of Particle Size - SB-C18 Column: SB-C18, 4.6 x 50 mm Mobile Phase A= 0.1% formic, B=0.1% formic in MeCN (92:8) Flow Rate =1.5mL/ Inj. Vol: 2 ul Detection: UV 254 nm Flowcell: 2uL, 3 mm flow path mau 60 5 µm α 2,1 = 1.36 α 3,2 = 1.27 α 4,3 = methylxanthine 2. 1,3-dimethyluric acid 3. 3,7-dimethylxanthine (theobroe) 4. 1,7-dimethylxanthine mau µm α 2,1 = 1.36 α 3,2 = 1.27 α 4,3 = Slide 12

13 Scaling - Easy Conversion from Analytical to RRHT Compare 5, 3.5 and 1.8 μm Columns mau Eclipse XDB-C x 150 mm, 5 um 6 ul inj. RED - Same from column to column BLUE Changes from column to column 0 mau Rapid Resolution Eclipse XDB-C x 100 mm, 3.5 um 4 ul inj. Sunscreens: 1. 2-hydroxy-4-methoxybenzophenone 2. Padimate-O 3. 2-ethylhexyl trans-4-methoxycinnamate 4. 2-ethylhexyl salicylate mau Rapid Resolution HT Eclipse XDB-C x 50 mm, 1.8 um 2 ul inj. Mobile phase: ( 85:15) MeOH: water Flow = 1.0ml/. LC: Agilent Slide 13

14 3. New RRHT Columns with Fixed, High-Pressure End-fittings for 600 Bar Use High-pressure RRHT 1.8um columns 150mm 100mm 50mm Fittings look different on 600 bar columns than those on 400 bar columns. Metal-to-metal internal sealing for high pressure use 400 bar RRHT 1.8um column Standard fittings, support typical pressures Slide 14

15 Optimized Hardware Design Makes it Possible to Inject Dirty Samples Sample: plasma sample, proteins precipitated, filtered 0.2um filter, only Minimum sample prep means faster analysis Typical hardware design plugs faster pressure Optimized hardware design does not plug easily. 50 Almost no increase in pressure over 600 injections!!! # of injections Slide 15

16 Short Columns for Fast LC and Long Columns for High Resolution Narrow Bore Standard Bore 1mm ID 2.1mm ID 3.0mm ID 4.6mm ID High-Resolution RRLC Ultra-Fast Binary Comparison of Supported RRHT 1.8µm Columns on 1100 and 1200 RRLC Systems Slide 16

17 ????? Let s break for questions! For questions, at break please dial 1 on your phone, or send a note with your questions. Slide 17

18 4. Verify Performance of RRHT 1.8um Columns Performance measures include: Comparison of Efficiencies vs. Rapid Resolution Column Van Deemter Curve Comparisons Achieving improved HETP for smaller particles Achieving well packed columns consistent and ideal reduced plate heights High Efficiency High Resolution Column Consistency for selectivity, efficiency, tailing etc. Slide 18

19 Comparison of Efficiencies RRHT and Rapid Resolution Columns High Resolution Ultra Fast { Resolution α N 1/2 Column Resolving Power Resolving Power Length N (3.5 μm )* N (1.8 μm) ,000 32, ,000 24, ,500 17,000** , *5 µm HPLC columns of the same length have 40% fewer plates (N-value); 4.6-mm i.d. ** Available as a custom column Slide 19

20 Van Deemter Curve 1: HETP vs. Volumetric Flow Rate HETP (cm/plate) μm 3.5μm 1.8μm Column: ZORBAX Eclipse XDB- C18 Dimensions: 4.6 x 50/30mm Eluent: 85:15 ACN:Water Flow Rates: ml/ Temp: 20 C Sample: 1.0μL Octanophenone in Eluent H = A + B/u + Cu Volumetric Flow Rate (ml/) Smaller particle sizes should have flatter curves, ima shift out slightly Slide 20

21 Van Deemter Curve 2: Dimensionless Plots of a Neutral Analyte on a ZORBAX Eclipse XDB-C18 Column Reduced Plate Height Eclipse XDB-C18, 4.6x50mm 65:35 ACCN:Water 40 degc Analyte is Hexanophenone 5.0 um 3.5 um 1.8 um Reduced Interstitial Linear Velocity Looking for all curves to overlay and reduced plate height to be about 2 This indicates a well packed column independent of particle size Slide 21

22 Resolution Improvements on Long 1.8 μm Columns 4.6 x150mm SB-C18 RRHT - 5µm vs. 1.8µm 5µm: Rs(4,5) = 3.63 Peak Width, 5σ (9) = µm: Rs(4,5) = 5.45 Peak Width 5s(9) = % Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) Rs = +50% Resolution improvements on long columns are just as good as on short columns Slide 22

23 Resolution Improvements on Long 1.8 μm Columns 2.1 x150mm SB-C18 RRHT - 5µm vs. 1.8µm mau um vs. 5um + 60% Resolution (Theory: 67%) + 160% Efficiency (Theory: 177%) Pressure at start: 51bar, 5µm Rs(4,5) = 3.14 Peak Width 5σ (9) = Pressure at start: 394bar, 1.8µm Rs(4,5) = 5.07 Peak Width 5σ (9) = Resolution gains are independent of column ID on a low volume LC Slide 23

24 Column to Column Variation Efficiency and Tailing Factor Eclipse XDB-C18, 2.1x150mm, 1.8µm Plates Avg plates = 32,500 Avg Tf = Column # Tailing Factor Plates Half Tail Factor Slide 24

25 Column to Column Variation Efficiency and Tailing Factor SB-C18, 3.0x50mm, 1.8µ Plates Avg. Plates = 10,612 +/- 350 plates (3.3%) Tailing Factor Plates Half Tail Factor Avg. Tf = / (1.5%) Column # Slide 25

26 Column-to-Column Variation Selectivity and Peak Capacity SB-C18, 2.1x100mm, 1.8µ Alpha Peak Capacity Alpha Cp Column # 0 Slide 26

27 5. Use RRHT Columns Routinely 1. In older LC s, like 1100 Series LC s a) Limited configurations can be used 50mm and shorter in general b) Ideal for fast and ultra-fast analysis 2. In new 1200 Series RRLC capable of 600 bar and 100 C use a) Allows for the complete range of RRHT 1.8um columns to be used b) Allows for temperature and flow to be optimized without pressure concerns c) Most extensive range of conditions for the most applications Slide 27

28 Use RRHT Columns Routinely Available for 3 Years Wide Range of RRHT 1.8um RRHT Columns 2003 Columns Available bar Length: 15, 30, 50 mm ID: 2.1, 4.6mm Bonded Phases: 5 Eclipse XDB-C18, C8 SB-C18, C8 Extend-C18 Length: 20, 30, 50, 100, 150mm ID: 1.0, 2.1, 3.0, 4.6mm Bonded Phases: 6-8 Eclipse XDB-C18, C8 SB-C18, C8, CN Extend-C18 Eclipse Plus C18/C8 More than 80 choices to meet any application! Slide 28

29 The Highest Flexibility: RRHT 600bar Column Portfolio and Introduction Dates over 80 choices to meet any application need Dimensions Eclipse Plus C18 Eclipse Plus C8 Eclipse XDB-C18 Eclipse XDB-C8 SB-C18 SB-C8 SB-CN Extend-C x x x x x x x x x x x x x x x x x Released Blue = May Yellow = July Green = August Slide 29

30 Use RRHT Columns with 1100 Series LC s Limited configurations can be used Pressures of 400 bar and less - targeted to very fast analyses with short columns (50mm and shorter) Analytical 4.6 mm and 3.0mm ID columns work well with slightly higher delay volume instrument 1100 Series LC s can be optimized for best performance with fast RRHT 1.8um columns Slide 30

31 Reduce Analysis Time by 80% Using Rapid Resolution HT Columns 1100 LC x 250 mm, 5μm R s (4,5) = 2.1 N = 22, A B C N= 11, N = 22,000 R s (4,5) = 1.8 Rapid Resolution 4.6 x 150 mm, 3.5μm R Rapid Resolution 4.6 x 75 mm, 3.5μm s (4,5) = 1.4 D E N = 6, N = 12,000 Rapid Resolution 4.6 x 50 mm, 3.5μm Rapid Resolution HT 4.6 x 50 mm, 1.8μm R s (4,5) = 1.2 R s (4,5) = Columns: ZORBAX Eclipse XDB-C18 Mobile Phase: 73% MeOH, 27% 20mM Phosphate Buffer, ph 7. Flow Rate: 1 ml/ Temperature: Ambient Detection: UV 254 nm Sample: 1) uracil, 2) naproxen, 3) mefanamic acid, 4) butyl paraben, 5) propranolol, 6) napthalene, 7) dipropyl phthalate Baseline resolution can be maintained while analysis time is reduced by 80%. Slide 31

32 8X Faster Analysis on RRHT Columns with Easy Method Transfer High Pressure not Required mau RRHT 4.6 x 50 mm 1.8 μm Flow Rate: 3 ml/ Pressure = 229 bar Column: ZORBAX Eclipse XDB-C18 Mobile Phase: 95% ACN: 5% Water Temp: Ambient Injection volume: 1 ul Conventional 4.6 x 150 mm 5 μm Flow Rate: 1 ml/ P = 37 bar Sample: Vita E α, β, γ-tocopherols in gel cap Eclipse XDB-C18 is a good first choice for many methods. Slide 32

33 Update USP Assay for Fenprofen Calcium (non steroidal anti-inflammatory (NSAID)) mau mau mau x 150 mm, 5 um 5 ul inj R = 17.3 N/m= R = 15.8 N/m= R = 16.5 N/m=73000 Rapid Resolution 4.6 x 100 mm, 3.5 um 3.3 ul inj. Rapid Resolution HT 4.6 x 50 mm, 1.8 um 1.7 ul inj. USP Requirements: L7 column, Rs >8 TF(5%) < 2.0 for each N=3000 or 20000/m Mobile phase: ( 500:496:4) acetonitrile: water: H 3 PO 4 Flow = 2.0ml/. isocratic Temp: ambient Detection: UV 272nm LC: Agilent 1100 Sample: resolution solution, fenprofen (peak #1) with gemfibrozil prepared as described in USP Tailing factor for each of these six peaks is <1.3 High resolution and exceptional efficiency maintained for low cost updating to fast LC methods Slide 33

34 ????? Let s break for questions! For questions, at break please dial 1 on your phone, or send a note with your questions. Slide 34

35 RRLC Instrument Considerations: Can the HPLC in your lab today handle RRLC? Things to consider on your system: Data Rate match to expected peak widths. Extra-column volume imize with short capillaries, 0.12 mm (red); Uv flow cell w/ smaller volume. Gradient delay volume switch to high-pressure mixing pump (e.g. optimized 1100 binary, new RRLC 1200 SL). Extracolumn volume Data rate Gradient delay volume Slide 35

36 Possible Instrument Configurations for Cost Effective Fast and Ultra-Fast HPLC 4.6 mm ID columns 0.17 x 400 mm capillary 0.17 x 200 mm capillary 0.17 x 105 mm capillary High pressure Gradient pump Std or well Plate sampler 3 μl heat exchanger Standard assembly without standard mixer and with 80 μl mixer Thermostatted Column compartment 0.12 x 400 mm capillary 0.12 x 200 mm capillary 0.12 x 70 mm 2.1 mm ID columns High pressure Gradient pump High speed Well-plate Sampler Column oven, 3 μl heat exchanger Standard assembly without standard mixer 0.17 x 105 mm capillary x 220 mm capillary x 80 mm capillary Rapid resolution HT Column Diode Array detector Waste Union DAD equipped with a 5 ul or 500 nl flow cell 0.1 x 300 mm capillary x 80 mm capillary and/or Column Diode Array Detector) DAD equipped with a 1.7uL or 500 nl flow cell 0.13 x 600 mm PEEK Mass Spectrometer * Pieces to upgrade Slide 36

37 RRHT-1100 Upgrade Kits Make Conversion of 1100 for use of RRHT Columns Economic first step in conversion for budget constrained users mm RR HT 1100 Fast LC Kit VWD mm capillaries, 80 ul mixer, 5 ul semi-micro VWD cell - three 4.6 x 50 mm Zorbax 1.8 um columns mm RR HT 1100 Fast LC Kit DAD mm capillaries, 80 ul mixer, 5 ul semi-micro DAD cell - two 4.6 x 50 mm Zorbax 1.8 um columns mm RR HT 1100 Fast LC Kit MS/DAD mm capillaries, 80 ul mixer, 1.7 ul micro DAD cell mm std ALS and WP ALS needle seats - two 2.1 x 50 mm Zorbax 1.8 um columns Slide 37

38 1100 DAD SL UFLC Flow Cell Recommendations: Match flow cell volume to chromatographic peak widths 13 µl Standard Flow Cell: For highest sensitivity and linearity mm ID, 3.5 to 5 um columns 1.7 µl Micro Flow Cell: For highest chrom resolution UFLC 1.8 um mm ID columns Flow Cell Volume/Pathlength Uv Signal /Noise Chrom. Resolution* 13 µl / 10 mm µl / 6 mm µl / 6 mm * Depends on analytical conditions and column dimension 5 µl Semi-micro Flow Cell: Best compromise of sensitivity and selectivity HPLC/UFLC, 1.8 to 5 um mm ID columns Other flow cells available: 500 nl for capillary LC 80 nl for nano LC 0.6 mm for Prep LC Slide 38

39 Use 600 bar RRHT Columns with 1200 RRLC Routinely The complete range of RRHT 1.8um columns can be used for High Speed and High Resolution LC a) Both long and short mm b) All column ID s can be used c) High speed and high resolution LC is easily achieved Allows for pressure, temperature and flow to be optimized Most extensive range of conditions for the most applications Slide 39

40 1200 RRLC Ideal for Use with RRHT Columns for High Speed and High Resolution LC 1. Increased linear flow rates for higher analysis speed Higher Column and System Temperature: 80 C => 100 C Higher Column and System Pressure: 400bar => 600bar Minimized column pressure: 25% Minimized system pressure: 30% at 1ml/ Maintained high flow rates: ml/ 2. Increased efficiency for higher resolution and peak capacity Longer columns: 50mm => 150mm By higher P & T and reduced column & system pressure 3. HPLC sensitivity, precision, resolution at high analysis speed u-degasser with continuous flow Binary Pump SL with new Electronic damping control (EDC) HiP ALS SL with variable loop, flow-through design and new µ-valve design TCC SL with 3x T-stability: +/ C => +/ C TCC SL with post-column cooling (PCC): Noise < 50uAU at high T and flow High Speed Detectors -DAD/MWD SL with 80Hz, VWD SL with 55Hz data rate, TOF with 40Hz data rate and high-speed QTOF, QQQ, IT, Quad Slide 40

41 1200 RRLC Ideal for Use with RRHT Columns for High Speed and High Resolution LC 4. High Method Flexibility i. Support of narrow ( mm) and analytical (3.0 and 4.6mm) columns Configurable delay volume (120ul and ul) ii. Injection volume from ul without loop change 1.6ul, 3ul, 6ul heat exchangers (heating/cooling) 9 Flow cells: 5x analytical (80nl to 13ul), 3x prep, 1x SFC Support of RRLC and conventional HPLC on one configuration Std delay vol. with ul, 0.17mm capillaries, 3ul/6ul heat exch. Column length from mm High flow rates up to 5ml/ iii. Broad Range of >80 RRHT 1.8um Columns Phases: SB-C18/C8/CN, XDB-C18/C8, Extend-C18 IDs: 1, 2.1, 3, 4.6mm Length: 20, 30, 50, 100, 150mm iv. Same Chemistry for 1.8, 3.5 and 5um particle columns fast, easy, secure method transfer 5. Uncompromised Robustness, Compatibility and Scalability Based on 1100, full compatibility with 1100, stepwise upgrade to RRLC Slide 41

42 High Speed LC with RRLC and RRHT Columns Maintaining Resolution at High Analysis Speed PW=0.30se c PW=0.33sec 80Hz 80Hz versus 10Hz (20Hz) Data Rate Peak Width: 55% ( 30%) Resolution: + 90% (+ 30%) Peak Capacity: + 120% (+ 40%) App. Column Eff.: + 260% (+ 70%) PW=0.42sec 40Hz Data Rate Peak Width Resolution Peak Capacity 80 Hz Hz PW=0.67sec 20H z 10Hz PW=1.24se 5Hz c Hz Hz Hz Sample: Column: Gradient:: Flow Rate: Phenones Test Mix Zorbax SB-C18, 4.6x30, 1.8um %ACN in 0.3 5ml/ Slide 42

43 High Speed LC with RRLC and RRHT Columns SB-C18, USP Method for Triamcinolone 250 mm, 5 µm Columns: 4.6 x (250, 50 mm) SB-C18 A= water, B= MeOH (40:60) F=1.5 Inj. Vol: 10 ul Detection: 254 nm Flowcell: semimicro Conventional Analysis Time mm 1.8 µm High Speed Analysis Time Slide 43

44 High Resolution LC with RRLC and RRHT Columns Column: 150mm RRHT SB-C18 Efficiency ~ 30,000 High Resolution LC Example from Pat Sandra & Frank David, RIC Slide 44

45 Using 1200 RRLC and RRHT Columns Explore Temperature and Flow RRHT, 150mm, 1.8um Temperature Comparisons 100 C Flow Rate Comparisons at 80 C C ml/ C P= 241 bar C P = 500bar ml/ C RRLC Allows Easy Studies of Flow Rate and Temperature with any Sample Conditions: Gradient Analysis of Sudan Dyes in Food Column: RRHT SB-C x 150mm Mobile Phase: A:Water B:ACN Gradient: 25% B 75%B in 30 utes, hold at 75% B for 5 utes Analytes: Sudan Red 1 4 Detection: VIS 520 Slide 45

46 Conclusions High performance with RRHT, 1.8um columns is easily achieved. The columns can be used routinely for fast and high resolution analyses. A limited range of columns can be used on existing LC s The new 1200 RRLC, with a higher pressure and temperature limit expands the range of columns for achieving high performance. The widest range of applications can be explored with high temperature and high flow rate. Slide 46

47 Agilent Applications Technical Support (phone: US & Canada) * * Select option 4, then option 2 for LC. * Select option 4, then option 1 for GC (fax) Slide 47

48 Wrap-up E-Sear Questions Thank you for attending Agilent e-sears. Our e-sear schedule is expanding every week. Please check our website frequently at: Or register for Stay current with e-notes to receive regular updates Slide 48

49 Upcog esears ZORBAX Eclipse Plus Solutions for HPLC Separations June 22, :00 pm EDT (US) 7:00 pm CET (Europe) Inlets Used in Capillary GC July 13, :00 am EDT (US) 5:00 pm CET (Europe) Migrating from HPLC to Ultra Fast and High Resolution LC July 25, :00am EDT (US) 5:00 pm CET (Europe) Slide 49