Biosafety in Lab. Cairo University

Transcription

1 Biosafety in Lab Cairo University

2 Second year student at Faculty of Science, Zigzag University Eman Said Attia died after (Al tnerugen) Experiment in organic chemistry lab. In appropriate preparation for laboratories Lack of experience in working safely with the dangerous chemicals

3 Why is Lab Safety Important? a Problem!!!! Active Learning

4 To use effective active learning techniques To introduce participants to the concepts of research mis-conduct related to lab biosafety by generating active learning environment.

5 5 th May 2016 Workshop Registration Fees : Free Attendance with certification deadline for registration : 21 /04/2016 request for application send to haderhesham90@yahoo.com

6 No of Applicants: 30 The Selected Participants including under-graduate, post graduate students and academic stuff (demonstrators, assistant lecturers, lecturers) Divide into 5 groups No of Participants

7 Pre-Assessment Test

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9 Pre- Assessment test evaluation 10 participants answered 15% of the test 8 participants answered 20% of the test 1 participant answered 50% of the test Only 2 participants answered 75% 12 Percentage of answered questions 10 Number of participants % 15% 20% 25% 35% 75% 50%

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12 Dr. Mohamed El-Shinawi give an opening talk entitled Beyond the Egypt educational institutes on Responsible Science Challenges and Steps to Success

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14 Objectives First Session (Presentation) An overview on safety in Laboratory By Hadeer Hesham Abdel-fatah Identify good practices in lab biosafety for all students, demonstrators, assistant lecturers, lecturers and researchers at Cairo university and develop more understanding of laboratory work related threats Define lab precautions. Identify importance of using safety symbols, emergency equipment and personal protective equipment.

15 Activity 1: Case study (5 mins) A student was heating a test tube containing a mixture of chemical liquids. Instead of heating the mixture gently, he heated it strongly without shaking. After heating, he immediately put the test tube under the nose of a girl student standing next to him for her to smell the gas evolved. Think in groups about safety violations and Emergency response.

16 Activity 2: Safety Picture comment (5 mins) What is wrong here? Write a lab safety rules for this case?

17 art What is wrong here? Write a lab safety rules for this case?

18 What safety violations do you see? How could the violation be fixed? What could happen if the violation is not fixed? ( 10 mins )

19 Activity 3: Safety Symbols Flashcard Game Photocopy and prepare sets of cards and give each group an envelope containing a set of cards. (10 mins)

20 Activity 4: Safety Poster Relate all following equipment and symbols with the following Situations with a poster. Design a poster including all the roles and safety symbols that should be followed first, type of equipment should be used and type of information should be known. Emergeny respond to situations including tools and symbols. Participate in groups all group members should be present (20 mins)

21 Activity 4: Situations 1. Hend was wearing headphones unaware that the beaker she kept on flam for heating start to burn. 2. Hamza was heating an acid when the acid start to spray and scattered sprays get into eye of the student stand next to hamza. 3. One of students start conducting a new experiment on a material that he never deals with, shortly and explosions started. 4. It was summer when incendiary material spilled on one of student's clothes.

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26 The second session presented Types of lab Hazards and necessary Safety by Dr. Sherif Ibrahim Lecturer of Cell Biology, Zoology Department, Faculty of Science, Cairo Uinversity Objectives for this session: Identify types of lab hazards Identify good practices in context of lab safety

27 Activity 1: Video about lab safety Discussion? Laboratory Hazards physical chemical biological

28 Chemicals should be stored in alphabetical order?

29 Activity 2: Procedures for handling chemicals? Discussion within groups (5 mins)

30 Activity 3: What do the following pictograms stand for? (10 min)

31 Staying safe means that you Read labels on containers of chemicals Read Material Safety Data Sheets (MSDS) Handle chemicals with care Use correct protective clothing and equipment Remember emergency procedures

32 Health Hazards on a Chemical Label Carcinogen Highly toxic agent Toxic agent Reproductive hazard Irritant Corrosive Sensitizer Hepatotoxin Nephrotoxin Neurotoxin

33 Activity 4: What is useful information provided in this sheet? (10 min)

34 Chemical Labels Every container of chemicals is labeled by the manufacturer. The label will tell you Name of chemical Name, address and emergency phone number of manufacturer Physical and health hazards Precautionary measures First-aid instructions Proper handling/storage instructions

35 Common Signal Words on Labels Danger Can cause immediate serious injury or death Warning Can cause potentially serious injury or death Caution Can cause potentially moderate injury

36 Physical Hazards Flammability Explosive Corrosive Reactivity Stability

37 Contamination Routes Ocular invasion Inhalation Ingestion Skin penetration

38 Biological Hazards Aerosols and droplets are the main sources of contamination Facilities and Safety - Module 2

39 Biosafety Practices Question 1 I am required to wear my lab coat in the lab during the experiment time only. A. Yes B. No C. Don t know

40 Question 2 A tube of pathogenic Salmonella broke onto the floor and you need to look for spill cleaning kit. It should be: A. Next to the lab door B. Under the safety cabinet C. Near the sink Question 3 You need to clean the Salmonella spill: A. Wipe with water, and then disinfectant B. Wipe with disinfectant and then water C. Don t know

41 Biological Safety Cabinet (BSC) The biological safety cabinet (BSC) is the principal device used to provide containment of infectious splashes or aerosols generated by many microbiological procedures. Biological safety cabinets can only protect the worker and the experiment if they have been properly selected for the intended containment function.

42 Question 4 The best way to dispose non-labeled items in the lab is A. Throw in the biohazard bin to be autoclaved. B. Disinfect with bleach C. Don t know. Question 5 You found the BSC is busy and you want to make a quick sub-culture of your salmonella. The best alternative is: A. Laminar flow B. Fume hood C. None

43 Third Session Biosafety in a Clinical Virology Laboratory Dr. Hossam Taha Mohamed

44 What is Biosafety A biosafety level is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4). Biohazardous microorganisms Biosafety containment levels Level 1& 2: basic laboratories Level 3: Containment laboratories Level 4 : high containment laboratories

45 Risk group classification Risk groups, biosafety levels, practices and equipmentt

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47 Transport of viral samples Triple packaging Treating contaminated laboratory waste

48 Closing Session General rules and recommendation Professor. Dr. Mona Mostafa Mohamed

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52 Percentage of answered questions 120% 100% 80% 60% 40% Post- Assessment test evaluation 12 participants answered 90% of the test 14 participants answered 100% of the test 4 participant answered 75% of the test 4 Number of participants % 0%

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