REAL TIME PCR DETECTION KITS

Transcription

1 REAL TIME PCR DETECTION KITS

2 REAL TIME PCR KITS Our company Certest Biotec is a Company focused on the development and manufacture of in vitro diagnostics for human, veterinary and agro alimentary applications. The company was founded in 2002 and its success is highly associated to three basic pillars, our human resources, innovation and customer orientation. These conditions allow us to provide our customers with products for clinical diagnosis with high standards of quality, using flexible solutions to ensure customer satisfaction. We are backed by our extensive network of distributors in more than 120 countries across the 5 continents. Molecular diagnostics Molecular detection techniques have revolutionized clinical diagnosis over the past several years. In particular, the real-time quantitative Polymerase Chain Reaction (qpcr or Real Time PCR) has been turned into one of the most powerful in vitro diagnostic tools for the detection and quantification of DNA or RNA. In this way, the Real Time PCR allows us to identify the causal pathogens of the infectious diseases by the use of specific primers and a fluorescent labelled probe that hybridizes to a specific region of the target gene.

3 Work flow VIASURE VIASURE was created with the purpose to provide Molecular Diagnostic users new real-time PCR kits for the detection of pathogens of interest. VIASURE provides maximum flexibility and compatibility with leading open system thermal cyclers and the latest technology for the stabilization of products. Components VIASURE Real Time PCR Detection Kits are designed for the diagnosis of infectious diseases caused by different pathogens in human samples. This new product range is based on 5 nuclease chemistry. This technology utilizes two primers and a hydrolysis probe and exploits the exonuclease activity of Taq DNA polymerase. During the amplification, the increase in the fluorescent signal is proportional to the quantity of target sequence present in the sample and could be measured on a wide range of real time PCR platforms. VIASURE Real Time PCR Detection Kit contains in each well all the necessary components for real time PCR assay in a stabilized format, which allows the shipment at room temperature. Reagent/Material Description Quantity Low or high profile 8-well strips* A mix of enzymes, primers-probes, buffer, dntps, stabilizers and Internal control in stabilized format 6/12 X 8-well strip Low or high profile 96-well plate* A mix of enzymes, primers-probes, buffer, dntps, stabilizers and Internal control in stabilized format 1 plate Rehydration Buffer Solution to reconstitute the stabilized product 1 vial x 1,8 ml Positive Control Non-infectious synthetic lyophilized DNA 1 vial Negative Control Non template control 1 vial x 1 ml Water RNAse/DNAse free Water RNAse/DNAse free 2 vials x 1 ml Tear-off 8-cap strips* Optical caps for sealing wells during thermal cycling 6/12 x 8-cap strip Shell Frame Grid* Shell Frame Grid 1 or 2 Benefits and advantages Ready to use Kits, containing all the necessary components for the testing. Long term stability. Transport at room temperature. Easy to use. It minimizes the number of manipulations reducing time and possible errors. High sensibility, specificity and reproducibility. It allows the identification of pathogens at the early stages of the infection. Low volume of sample, 5 µl. Versatile. Possibility to analyze from 1 up to 96 samples per testing. Flexible. It can be adapted to a high number of commercial thermal cyclers. Exogenous internal control. Evaluated and validated according to ISO and CE marked. *Components according to thermocycler and customer requirements.

4 Compatibilities LOW PROFILE BLOCK THERMOCYCLERS HIGH PROFILE BLOCK THERMOCYCLERS Manufacturer Agilent Technologies Roche Roche Model AriaMx Real-Time PCR System 7500 Fast Real-Time PCR System 7500 Fast Dx Real-Time PCR System QuantStudio 12K Flex 96-well Fast QuantStudio 6 Flex 96-well Fast QuantStudio 7 Flex 96-well Fast StepOne Plus Real-Time PCR System StepOne Real-Time PCR System ViiA 7 Fast Real-Time PCR System CFX96 Touch Real-Time PCR Detection System Mini Opticon Real-Time PCR Detection System LightCycler 480 Real-Time PCR System LightCycler 96 Real-Time PCR System Manufacturer Model 7300 Real-Time PCR System 7500 Real-Time PCR System 7900 HT Real-Time PCR System ABI PRISM 7000 ABI PRISM 7700 Eppendorf Stratagene-Agilent Technologies Stratagene-Agilent Technologies Analytik Jena Biometra QuantStudio 12K Flex 96-well QuantStudio 6 Flex 96-well QuantStudio 7 Flex 96-well ViiA 7 Real-Time PCR System CFX96 Touch Deep Well Real-Time PCR Detection System icycler iq Real-Time PCR Detection System icycler iq 5 Real-Time PCR Detection System MyiQ Real-Time PCR Detection System MyiQ 2 Real-Time PCR Detection System Mastercycler ep realplex Mx3000P Real Time PCR System Mx3005P Real Time PCR System TOptical Analytik Jena Biometra qtower 2.0

5 CERTEST Biotec, S.L. Pol. Industrial Río Gállego II, Calle J, Nº 1, 50840, San Mateo de Gállego, Zaragoza (SPAIN) Real Time PCR Detection Kits Version: EN 1501