Innovative possibilities for enzyme immobilization via plasmatreated. activities, stabilities and multiple-shift usage

Transcription

1 Innovative possibilities for enzyme immobilization via plasmatreated surfacesimproved activities, stabilities and multiple-shift usage Ulf Menyes, Karsten Schröder, Marko Häckel, neoplas GmbH, Greifswald Germany Torge Vorhaben, Dominique Böttcher, Uwe Bornscheuer, Institut of Biochemistry, University of Greifswald Applications, Consulting, Services

2 neoplas an INP Spin-off company The only Institute GmbH of Leibniz Association. Amtsgericht Stralsund Handelsregister HRB 6248 Founded on November 29, 2005 Scanbalt Forum 2010, Tallin Estonia, September

3 Leibniz Association 86 non university members 14,000 employees, 5,300 scientists 1,600 postgraduates ca. 1.1 Mrd. total budget 205 Mio. third party budget duties with national importance jointly funded by states and federal governments Scanbalt Forum 2010, Tallin Estonia, September

4 Facts and figures Focus on Exploitation of scientific research results Applied plasma technology and its applications in life sciences Specific Technology Transfer Development of prototypes into market-ready products, CAD design, construction Efficient Technology Management Market analysis, (EU)project management, consulting, funding, business incubator Visible Technology Marketing Development of technology networks, profit-oriented marketing of research results Scanbalt Forum 2010, Tallin Estonia, September

5 Interface neoplas From Research FROM PROTOTYPE TO PRODUCT SERVICES To Market Laboratory samples, Patents and Prototypes neoplas GmbH Production & Distribution Companies Scanbalt Forum 2010, Tallin Estonia, September

6 Core comptences - plasma technology Scanbalt Forum 2010, Tallin Estonia, September

7 Prototypes into market-ready products PlasmaJet for surface treatment of complex 3D-shapes, sub-mm capillaries, fine holes or slits for surface activations and improved bonding of polymers Scanbalt Forum 2010, Tallin Estonia, September

8 Micro plasmajet Miniaturized plasmajets - Sources for localized surface treatment Application areas Medicine Pharmacy Microbiology Surface technology 40 mm Applications: Increase of wettability or printability Removal of chemical additives Deposition of functional layers Active principle: Capillary discharge system with gas feed through, non-thermal High frequency excitation Atmosphere pressure Scanbalt Forum 2010, Tallin Estonia, September

9 Heart catheters - sterilisation by plasmas Scanbalt Forum 2010, Tallin Estonia, September

10 Plasma-technical services AFM, FT-IR, XPS, AES- Measurements Analysis of surfaces Analysis of fluorescence lamps Plasma diagnostics Diagnostics on Plasma-Blaster/Jet Powder and nano particle coatings Surface refinements Coatings of PE-tubes Scanbalt Forum 2010, Tallin Estonia, September

11 innovative plasma technology for enzyme immobilization corporation project between three partners founding from Ministry of education and science other industrial partners in future Scanbalt Forum 2010, Tallin Estonia, September

12 innovative plasma technology for enzyme immobilization work group Prof. Uwe T. Bornscheuer Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis Greifswald University Access to biocatalysts Identification of new biocatalysts by screening, cloning and functional expression in suitable microbial hosts Creation of tailor-made enzymes using directed (molecular) evolution rational protein design Mutant library creation Biocatalyst with e.g., high stereoselectivity Transfer / Cultivation / Expression Biocatalyst Engineering Cycle Measurement via High- Throughput Screening Relative Absorbance 340nm 1 0,8 0,6 0,4 0,2 0 O O Time [s] O E~30 O Scanbalt Forum 2010, Tallin Estonia, September

13 innovative plasma technology for enzyme immobilization purpose implementation of plasma processes for customized surface modification for enzyme immobilization expansion application area of biocatalysts simplification biocatalytical processes of synthesis determined recovery and multi usage of biocatalysts cost-efficient application of biocatalysts Scanbalt Forum 2010, Tallin Estonia, September

14 innovative plasma technology for enzyme immobilization results (selection) Determination of pore structure and specific surface area using REM and BET-Method EP 100 MP 1000 BET Surface area sq.m/g BET Surface area sq.m/g x250 Adsorption BJH Pore Size Distribution Pore Dia. Pore Volume % Range(nm) (ml/g) Under Over BJH Total x250 Adsorption BJH Pore Size Distribution Pore Dia. Pore Volume % Range(nm) (ml/g) Under Over BJH Total x1700 x1700 Scanbalt Forum 2010, Tallin Estonia, September

15 innovative plasma technology for enzyme immobilization results (selection) successful immobilization of two lipases (Candida antarctica Lipase B und Geobacillus stearothermophilus Lipase) and two esterases (Pyrobaculum calidifontis Esterase und Sulfolobus tokodaii strain 7 Esterase) treating polypropylen with oxygen plasma immobilization yield increased up to 5-fold for Candida antarctica lipase B (CalB) 14-fold for Pyrobaculum calidifontis esterase (PestE) Activity [TBU/gsupport] Activity [TBU/gsupport] Applied activity [TBU] Applied activity [TBU] Immobilization on Accurel MP1001 at different enzyme loadings. Immobilized on oxygen plasma-modified support (dark grey), immobilized on untreated support (light grey) Scanbalt Forum 2010, Tallin Estonia, September

16 innovative plasma technology for enzyme immobilization results (selection) repeated use of immobilized CalB on MP1001 in the kinetic resolution of α-phenylethanol. CalB immobilized on oxygen plasma-modified support (black), Novozym 435 (striped), using untreated carrier (dark grey) or free enzyme (light grey) Conversion [%] Cycle number Vorhaben, T. et al. ChemCatChem 2010, 2, Scanbalt Forum 2010, Tallin Estonia, September

17 innovative plasma technology for enzyme immobilization results (selection) repeated use of immobilized PestE on MP1001 in the kinetic resolution of α-phenylethanol. PestE immobilized on oxygen plasma-modified support (black), lyophilized (dark grey), or using untreated carrier (light grey) Conversion [%] Cycle number Vorhaben, T. et al. ChemCatChem 2010, 2, Scanbalt Forum 2010, Tallin Estonia, September

18 innovative plasma technology for enzyme immobilization patent applied priority date: reference number: we search for partners from pharmaceutical and chemical industry corporation with enzyme producers research partners for application projects partner for introduction on the marked Scanbalt Forum 2010, Tallin Estonia, September

19 Contact us neoplas GmbH Address: Walther-Rathenau-Straße 49a, Greifswald, Germany Telephone: / , Fax: / contact@neoplas.eu, web: Scanbalt Forum 2010, Tallin Estonia, September

20 Contact us neoplas GmbH Walther-Rathenau-Straße 49a Greifswald, Germany Dr. Marko Häckel (CEO) Tel.: / Fax.: / marko.haeckel@neoplas.eu marko.haeckel@neoplas.euthanks for attention! Dr. Ulf Menyes Tel.: / Fax.: / ulf.menyes@neoplas.eu Scanbalt Forum 2010, Tallin Estonia, September