Supplemental Figure 1 Real-time RT-PCR analysis of 16 genes other than those shown in Fig. 6C.

Transcription

1 arbitrary At1g02813 unit expressed protein Relative mrna amount (X3.7) (X118) (X1.7) 5.6% act At1g06990 GDSL-motif lipase 3.0 (X2.2) /hydrolase (X39) (X1.3) 2.8% act At1g23590 expressed protein (X1.5) (X1.9) (X1.7) 5.7% act At1g23610 hypothetical protein (X1.3) (---) (X2.7) 4% act At1g32180 cellulose synthase family 3.0 (X2.1) (X5.5) (X0.7) 0.59% act At1g47980 expressed protein (X1.7) 3.0 At1g68875 expressed protein (X2.1) At1g75940 At2g41040 glycosyl hydrolase family 1 methyltransferase-related 3.0 protein/ata (X2.1) (X4.3) At3g20450 expressed protein (X0.95) (X5.5) (X2.6) 12% act (X21) (X4.3) 59% act (X15) (X2.6) 9.0% act 3.0 (X0.3) (X3.8) 25% act 0.5 (X1.7) (X1.5) 2.9% act At3g25050 endo-xyloglucan transferase (X1.8) 1.5 At4g22080/At4g22090 pectate lyase family At5g13380 auxin-responsive GH3 family protein 1.5 (X1.1) At5g44400 FAD-binding domaincontaining protein 3.0 (X1.9) At5g57810 senescence-associated protein related 1.5 (X1.1) 0.5 (X0.87) (X7.2) 0.5 (> X45) 0.5 (X1.3) (X3.5) 49% act (---) 1.5% act (---) 04% act (X4.9) (X1.1) 4.9% act (X3.2) (X0.6) 0.32% act Supplemental Figure 1 Real-time RT-PCR analysis of 16 genes other than those shown in Fig. 6C. The induction system is described in the legend of Fig. 6C.

2

3 Supplemental Figure 3. Multiple sequence alignment of MS1 homologs (In ALN format). CLUSTAL X (1.81) multiple sequence alignment MPTARRRPARRRASESESASETSDDDGTASRSTELARTSSRSPLRSRATR EERRERGFVGRVVGALTPANWGFRLGAAREESSESDDESAEESESDEEEE MPSMR MAVMNGGRATKRARRSNRISADLYDF RPMKRARRNNRVTANLYDI MVVNGRPIKRMKR--RVTADLYNF MVVNGRPIKRMKR--RVTADLYNF MVVNSRPLKRART----RVEARDF MAVRRRGASAVAVHGGGGRVPKRARVTAEPSLLDV SEGDEDSDSAGTSKSDASAASTSSEGARVEEQEARRRRSANGTTTKASDE MPVPIIETCRKRKR MAITAYDALTERQK MAITTTIFEACSKKRK ALERLASWPTPPPPPPPPPPPAKAAAAEAERPCPPSSAATTSSSSSSSAS MANLIRTDHQQHIPKK KK MAPKMVISLGSSR STFPTAEINGE STTLPPFRDGVRTFLATHARVTFPPST- LSFPSADDS VSPAVPFRDNIKAFLSRHARVTFPPS-- LSFPSSPSSSSSSSSSSP----PPPRGPFRSNIRSFLTEHALLPPPSS-- LTFPASSSSSSSSSSSSSSSL-PPPRGPFRSNVRSFLTEHALLPPPSS-- AGFPPAGDG GAAGTFREAVRGFLARYARLLPLPSIF RAFPGQKEEGEKQPARGGGKKSSSSSSTFRAQVRGFLARCAVAVPASEAG DENPKWRSDNVVKEPTPWISRDNSYSGPFRDNVKSFLNRYAACDGKYTEF -KPKVYNLQRFGEDGFP-----IQRNGAFRDQIRVFLRDCAEIEDYDIRG APKVLLINDFAISSSSSSPPFFNLAATFRDNIRSFLREYAEIEDYTIDG GSPLISTFNNSCSPPRPPPPPMPLHSGPFRDNIREFLKHCAEIEDYTVCH AAPAAAGAPRVYPLRDFPGREAAGLGGAFRDNVRWLLRQWGGASPASPAA RKRGESRVFRLKTFGESGHPAEMNEL-SFRDNLAKLLEFGHFES--SGLM RKR-EERVFRFKIFGENGYPVEFDG--SFQQNIKKLLELGHFQRNICSRM RRKRGEMLFRFEAFCQPGYPANFAGAGGFRDNVRTLLGFAHLEAGVHGET 1

4 ------LFSSLMTWQIMLRPGD-STDGS-DLSSKLVSLDVVEEDVTRSSR LFPSLLTWQILFRIGDLVLDGP-DISSVVLALDIVEEDVTRSSR LFPHLLTWQISFQVGDLVGCGGAEAGGGVVSLDVVEEDVARS-R LFPHLLTWQISFRVGDLVESGGGEAGSAVVSLDVVEEDVARS-R SPAAAAAPPHLLTWRVSLRVGE---EGDEEGGGGAVELNVVEEDVLRS-R -----ELSPGMSSWHVGFTTAG GEAVAVMEVVEEEVARA-R G IMGYTVTLSKGE PHETTLRIYEEAMAES-E MTVWCTLLSHETKS SLIPLYIVEENVKHSS WCTLLVIESKN FVVPLYTIEEDVKESV ATVSTIFLGSQANG VVFPLYIIEQQISDSSP NPVWCALLLSDSTG VVFPLYVLEENIHSSHP GGPGSASAAVAVAWRVLLSDDRTG VVVPIFAVEEPVASSP- G SWSFQLEIQRNPNP LYVLLFVVEEPIEASL- P SWSFKLEVIRQPS FHILLFVVEEPIEASL- K CWSFQLELHRHPP TVVRLFVVEEEVAASP-. : *: : : SVYCEHCCVVGWSSHPVCRKRYRFIIRSG------GDTKACTRCGNTQNL SVYCNQCRVVGWGGHPVCKKRYHFIIRANNSSPADGYQRSCNRCGNRCCF SVYCDQCRVVGWSGHPVCSKRYHFIIKADGNSIG-GYHKPCTCCGDVLHL TVYCDQCRVVGWSEHPVCSKRYHFIIKGDGNSIG-GYHKPCTCCGDILHL SVYCDQCRVVGWSGHPVCGKRYHFIIENDNNQVCGKRHSCCLRCG-TPTV RVYCEHCTVAGWSKHPVCSKRYHFIIRNENET---GTSKTCRRCGFMVAL RPHCDCCRCIGWVHHPVNKRQYHFIVHTDFKARS-----KPELAGKRICQ EPYCDHCRCTGWSNHFVSKRKYHFIIPIDT RPFCDLCRCNGWSHNSVSKRKYHMIIPVDS NPLCDFCRCFGWGHHYVTKRKYHMIIPNRD RPLCDRCRCIGWSHHFVSKRRYHLIIPKDD KPLCDYCRWAGWSHHWVSKRKYHFIIPAPA NLRCNHCQYVGWGNQMICNKKYHFVIPSKE EHHCKHCQYLGWGQNMICNRKYHFVLPSKD HRQCHLCRHIGWGRHLICSKRYHFLLPRRESAAE AD *. * ** : : ::*:::: SEGSNCKWCSMAL---DIENWVYSQLEDNTHLLHGVIHSNGYAHLLCLNG CGGCRCKGCDTVITTDDIEDWVYSQFEDNTHLLHGVVHSNGFGHLLLVNG SE-SRCKTCNHVTTADDVEEWIYHQLEDTTHLLHGVIHANGFGHLLRVNG SE-SRCKACDHVITADDVEEWVYHQLEDTTHLLHGVIHANGYGHLLRVNG AGESRCLLCNFDMDGEELEECGYMHLDDNTHLLHAVVHANGYGHLLRVNG QETRCPSCNHVGLSRDDPEDWDYVQLESPRHLLHGIVHENGFGHLVRING LCCCAVSASERKCSVCDEVDKECSILDHQTHLLHGLIHANGFGHLKRVNG EWSLPLEDDAFD-SQSHVLHGLIHCNGFGHLVCVNG EWSQKLEDGVCD-LQTHLLHGLIHCNGFGHLLCING EWNEPLKRESLT-LSSHLMHGLIHCNGFGHLLCINT QWNKPLNKESLE-KNNHLLHGLIHCNGFGHLLCING DWDRQLAADVILGRTDHLLHGLIHSNGFGHLVMLRG -TMAAFLKLEGGGYAFPEKESFSHLVELQGHVLHGFFHSNGFGHLLSLNG -IEAAFLNCQDG--AISTKDNFN-LVQSRGHIMHGIFHSNGFGHLLCVNG GLCFAINHGGGGGAEKASSKGTTTTASSRGHLLHGVVHLNGYGHLVALHG *::*...* **:.** :. REGGSGFLTGRAIMDFWDRLCSSLAVRKASVMDVSRKY-GMDYRLLHGIT REGGSSVLTGSDIMNFWDRLCETLAVRKISVMDVSRKY-GMEYRLLNAIT KEGGSRVLSGVHIMDFWDRLCKTLGVRKVSVMDVSKKY-GLEYRLLHAIT KEGGSRVLSGCHIMDFWDRLCKSLGVRKVSVMDVSKKY-GIEYRLLHAIT REGGSRCLTGRDIMSFWDRLCKVLHVRKVTVMDISKKH-GMEYRLLHAIT REGGSSVMTGSQLINFWDRLCRYLRVRKVSVIDVSKKY-GADYRSLHAVA REAGSMTLSGAQLMGLWETICYQLRAREVSVEDVSQKY-GVELRLLNPVS MESGSKYLCGREIVDFWDRLCNSLGARMITVEDLAKKR-SVELRLLYGVA REGGSKYLCGREIMDLWDRICASLRTRKITVEDVSKKR-SMDLRLLYGIA DLDDPNHLSGDQIMDFWDRLCSTLHTRKISLDDTSKKG-AMDLRLLHGVA IEANSKFLHGKELMNLWDHLCSILQTREISVEDLSKKAGSMDLRLLHGVA RDGGSTALSGRDIMDIWDCLCSALRARAVSVVDFSQKR-SLDLRLLLSVA IETGSDLTG-HQVMDLWDRLCTGLKARKIGLNDASHKK-GMELRLLHGVA MEGGSNLAG-CQIMEFWDRLCTGLRARKVSLNDISQKR-SMELRLLHGVA LEGGSDFVSGHQIMDLWDRICSALHVRTVSLVDTARKG-HMELRLLHGVA.. :: :*: :* *.* : * ::* : * * :: 2

5 RGCSWYSEWGYEFKSGSYALTKEAYQSAVDTLSAIPLSEFLF KGHSWYGGWGYEFGCGSFAITLDAYKKAVESISSVPLAPLLF KGHSWYGDWGYEFGAGSFGLTVYAYKSAVETLSSLPLSIFLS KGHSWYGDWGYEFGAGSFGLTVDAYKPAVETLSSLPVSIFLS SGHPWYGEWGYKFGAGSFALTSDTYQEAVDTLSGIQLALYFS TGCSWYGLWGFKLSSGSFGITPQDYSKAVESLSSVPLSNFFP RGATWYGTYGYIFGKGSFGNTLMTHKRATELLRKFPLRQLRNDFVIVKDL YGHSWFGRWGYKFCCGSFGVTKNEYENAIEALGSLEIDQIEFDFGELR-- YGHPWFGRWGYKFCHGSFGVTEPIYFKAIEILSSMELEKIIQDFSDTS-- YGRPWFGKWDYMFSHGSFGVKKDLYWRAILTLSSIEVDKILEELSGTS-- YGRTWFGKWGYNFSRGSFGVTQQKYERAIQILSSLDVSKIIHDFINKR-- HGDTWFTRWGYCLARGCFCVSTSTYAASVEALAALPVDYLR KGEPWFGRWGYRFGSGTYGVTQKIYEKALESVRNIPLCLLNHHLTSL--- FSEPWFGRWGYKFGRGSFGVTQPMHQKAIETIQGMPLCILVHYLGNS--- YGETWFGRWGYRYGRPSYGVALPSYRQSLHVLGSMPLCVLVPHLSCF---..*: :.: : : : :. : QGRKPRTQLHSIISFYQSLSCSELVTVKDLFSFLLQMIRENSSK RGRGPQTRLQALISFYRSLSDSELVTIKDLFSFLSSLIHASNES EGQKLQTRLLDIIKFYQSLSDRELVNIRDLFCYLTSLIHDAHKS EGQKTHTRLQDTIKFYQSLSDHELVNTRDLFCYLTSLIHDAHKS HRQPIRTPLQNTIALYWALSDRQLVTVRDLFRFIMHLLHQARKK HTRSPRNQLQNTIAFYQSLSRHPLTTIRELFAYIMELATG---- LDKEKKEGDVDMIELVDKYITVLGKAGVAINTLGELVNQVMKLQRDIRLA QSKEINQVFRYYREMSEGHLKTFRDLLRFMLIIKSHASPQ LSKSIKQIIHYYKDLSPTQLITFKDLLRFMLAIRSCPCVW KGRVMKKIIDFYRGSTESPLATLSDLLRFMLGFISKAPIE QGELVKRIINIYRDASETRLVTISDLFQFMLALNSTPLIR SRHVRRVVTIYRRLSNKPLVTVREFLRCLLDWKHLEPPL NRETPILLSKYQSLSTEPLITLSDLFRFMLHLHSRLPRD NHHIPVILSRYQTVSDHSLVTLGDLFRFMLELKTHLPEE SQELPMVVTKYQAISGHKLLSLGDLLRFMLELRARLPAT : :. ::. : P L PSR TSG NE VKKGQRAPERLALHGVAAVLAGVEVIEKGAEYKRNVTDSPKMARRPPPSG KLLPVTPPLLTDSPHQKRSSRL LLKKSDVADN KKQSMTATTTSKPPINIVLRRK PLIK RKTAMALVAMSLDHVSYPTLRADENSEVCTSPDQESDDNGYESGRDTVLD RKIALTLAAIPSKSSTHSAQQP----ETCLFKD QLPPVKTCTRLQFMLPKPSVMKR NYMSNSRNQII NCVDS SVTAMDYRG EPSAKKMKTNLAPTQPSAPPRTERARALRPREEETPPNSAQRQLKINLPS DHNT T

6 ----ASKS SVLCAWSKSDVERVQQTMVKILKASGRP--QAN ----ASKATLKNLKPAANILCAWTRDDVERVQQAMIKVLAAASG---KTN ---VNDSSCKKRCIYASGISSSWSKSDIERVEEAMFRVLRAVSG----SN ---VDDSSCKKCPFYAPGISPSWTRGDIERVEEAMFRVLRAVSG----SN ---TSKPTTDEHKEVASNVLCKWTKEDIDRAETAMLKVLRVVQP----GQ -----KSVHKEKMAYDANYQEEFPEEELQRATNIALKILRAADR EAMVQWDARARDTRSLPASASRWSEARLNVAANACVLALRDAQN-----K ---DKSPKYRNYSTVAANLGSRWPVRRLIFAAEVIVESLKEMKALKQN-- ---EKCMKYRNFSSLVGTMDSRWPTRRLQYAAEVIVDALKAKKEDKHSQE TSGIKPPQYYSFDDLSRREHSRWPGRRLNDAAQAVLKVFKERNS PNHHSSFSFIAKFDESRWPARRLDDVVRVVLTTLEANGS HRQQPCQRFEDVIDLLQCRWSKKRLLDAAEVVVDRLLEHGSG----A SIDSTNCRWSQKRIQMAIKVVIESLKRVE-----YR HIVKPTCRWSPKRVEMATRVIVEALKRAE-----FR IMSEASCRWSAKRVDMAARAVVDALRRAEPA---AR :. :.. : WVTRWALKR-SICKSASP-QLIDYCLKHFGGVLVDDGS---RVVSSRCNP WVTRHALKG-VMCKTASP-ELLDYCLKHLGGKLAASG----TMVWARCNP WVSWRALRG-AVCKVAPP-ELLDHCLKEIGGKFAADG----MIVRSRCNP WVSWRALRG-AVFKVAPP-ELLDHCLKELGGKFAADG----MIVSSRCNP WVSWRALRG-AASKAVDSQELLDYSLRGLGGKLMDDG----HFIAVRCNA WVATRTLKA-VTSHPIGSPQLVDYCIKTLGGTRTDDG----MVVAIRCNS WISRQEVRSRAREKGIGDTGLLDHILKTVNDVKVILPSGERGIVLRRQGA GMTRQDVRD-SARLHIGDTGLLDYVLKSMNNVVVGDVLVRRYVDPITRIL GMTRQDVRD-AARMHIGDTGLLDYVLKSMNNVVVGKYVVQRAVNPKTRIL TISRQDLRE-AVRSSIGDTGLIDFLLKHIDKVLIGDQIVQRSTNPKSRML KMDRQTLRD-AVRQHIGDTGLIDFVIKNIDKVAVENRFIHRVVNPVTRKL EMTRQAVRD-AARGAIGDTGLLDFVIKSLGDTIVGNYIVRRVPDAETRVL WISRQEVRD-AARNYIGDTGLLDFVLKSLGNQVVGNYLVRRSLNPVKKVL WVSRQDVRD-AARAYIGDTGLLDFVLKSLGNHVVGNYLVRRCLNPVTKVL WVTRQEVRD-AARAYIGDTGLLDFVLKSLGNHIVGNYVVRRTMNPVTKVL : :: *:*. ::.. GSNDFEYR LESVNNVHRLSNQDV-----N NSFDIEFRX LEPPSFKHNGNGLDS-----N SSGAFEYR LEPGNPSLN-SPATTGSSVIT SSGAFEYR LEPGNPSLNSTAAAIGSTVIT ETSAIEYR LEDNSNQSVDAAAFG EMNTVEYRL TTETLFPPNNVCTLT TQMEFTLDLSSKQQANGDVIMTTIKKDTNVEVRTESLAHCRATHSARSID HYTIQDLDDA VKAKEPKKKEAVVLEEITP-LRILTP EYSIDEFGDG IIP VKSEPESE-TVPAQP QFSLRTINSR VQEQKRKKKRKVKPQETS----ECTS VISLQDVVHE GKSEK KMESHA----DIPA HFSLEEYEEP TPALLD------VEVECTP-LPPVVR EYSLEDISNL LPSSNNELITLQNQNSMGKMATNGHN EYCLEDVSNV HPEQQGLVINNSK MKGRY EYCLEDVSSV LPAVAAGGGVPAQG------KMRVRF NASVEHVKQDLRYLYETLLHPQTMAEFRSR------ATREKMIDAATKIL QPSKEHIIGDLKFLFDSLVHPETMVNYRPL------VTREDVIDSATKLL CPSEGNLIQDLRFLYDNMLHPQTMMSYGPE------ATSDA-ISSARKLL CPSEENLIQDLRFLYENMLLPQTMLSFGHE------VTSDAGIISARKLL -PSVDHLLHDLKFLYNALLNPETMLASQPE------VIGASSHSAAAKIL ---QDHLFRDIKFLYDALLYPYTMHPYMPE------ENYQHAKRSAMILL ELTAAEVDRDLLALYHDVLENYKPARAQQKDGQRVTVKGAPLVDAARILL LKPGADVYGDLLLLYTNVLLNYPESEL VRSATQAIL LLPGADVNADVVFVYENVLFNYPESEL VEVATQAIL TTPGLSPYDDILYLYQNLLLTYPDSDL YSEASQVIL LEPGLDVNKDLHFLYNYVLLGYPDSHS VTLAIRAIL WPSTVEVERDLRAVYR------AMVEV RSEAAQAVL KITRGQVMKDMFYFYKHILMDYKGVLGPIG------GILNQIGMASRAIL RMTRPQLMKDMLYLYRCILKDQKPTMN--Q------GILSAIPAATRIIL QLTRAQLMRDLVHLYRHVLKEPSQALT--G------GAFGAIPVAVRMVL *:.: : :* 4

7 DCKHFIKDYLSS TVNP DCKQFVKDYCRPDKR MVDNP DCKQFMKDYNKCETTS SPSNP DCKQFMKDYNKCETT VASVP DCKQFIKHYDQHTPR APLNP DCKQFIKHYDLEEDF LPQNP DTKQFVKIYTAVDDLLDTTPASKLTKPAANAQKAIRIIATAVIHTRSQGP DSKHFIKEWPIWDN NDTVLQ DSKHFVKEWPFRVE NDQLLS KCKSFVKEWSYQ EQNHLT DSKHFAKQWQFKGNND HEDSFLG DCKHWVKWWGLGDE SDDQLR DAKYFIKEYHYIRDTSAKTLHLD RGE-ELG DTKYLVKEYNGELPWKIHHTGHE EAG-KMT DIKHFVKDYHEGQAAASSNGGGG FGHPHIN. * * : VAINLWCCVELSDELKE-SPAPPPERLVLPLNATVSDLKIEAAKAFQEVY FAIQLWCYVEFSEHLKE-DPTIPPELIVLPSNATVADLKTEATKAFQQVY PTICLSCQVEIVDQLEENVPDLPPEIIVLPSNATVFDLKLEASRAFQDVY NTIFLSCEVEIVDQLEENVPNLPPETVVLPSNATVFDLKREASRAFQDVY FLLSVRCSIELLDHPKD-YTAPPVELVLLPASATLAELKIQATRAFQETY SQLHIWCQVELADQVDD-PPCIPAELITLPQAATIADLKGEAARAFRDIY APSGLGPNVAKSRQKIGTITRPPPELVFLPSEPTLGDLKRVTSKAFRDLY FLCRINPSLVDVRSEQTT-ELPPGELVTVPLQATVYDLKQAIEETFRDTY FICQVMPTWNDLEAKFHR-KAPPGEIIVLPLHASVLELKQEAESALRDTY VSCQVLPNHEELLRDFTR-LLPPGDLVAVPENATIRELKSAAEKVLRDTY LVLRLRPSYNELVKELTR-PLPPGELLVVPQYATVDELKLMVQCALRDTY FFVEWQPQPWEA-AELIR-PMPLGEIVVVPLHASMGELLVESEHALRDTY IFCTIAWKCHHHNNEIK---VPPQECIVVKKDATLSEVYGEAERVFRDIY LYCTVFLRDKQESNEVMKKAMPPLECITLKNNATFNELKLEVERKFRELY LCCTLLVSNGSPE------LAPPYETVTLPAHATVGELKWEAQRVFSEMY : : :.:. :: : : * AMFKRFEVEELLGYGSIDDSITLKFLVGTNGVIRIKGRCS AMLKRFEARELVDYGPLEDSITIKFLVGQSGTVRIKGTCPS LMFRRFHAEELLGYSGVDDSTQVKLLIGSTESVRVQGRCLG LMFRRFHAEELLGYSGVDDSTQVKLLIGSAESVQFRGRCLG LMFQSYQVEQLPDFPNFSDTTLVKHVLGSSQLVRVRGRCTGD LMLQSFVADQLLDCATASDTTQIKLLFGAKGAVHIKGRCIGG VVLSDFKVTRVKGHEGQSDKTRLGWRKMHGASVEVQGDGAD CILSNFVVTEIDE--VEEDMS------LIGSCSALTVRG CMLERFVVIEIEH--MENLDDKDLLCKFVESGAEIFVKG CITETFEVLEIRNRYLEK--LDDNVSLKSQGNTEFMVKG YVMDKFAVKDVQIGKIEEKEYQDGVMCEVEQGVQVWVRG YFFEEFQAEGLHG--IAGEKWDPVMLGGAENGDTISVYG WELRDVVVESVVGGQIEITRVDEM-ALNGNKGLVLEGNVGMMMN WGLKSFVVESIMNLNVKGTDLVSE-VVEVGQKIVLEGSNAESGT LGLRSFAADSVVGVGADQEGLPVLGLVDVGSAVVVQGSVGEQINGEDHER. :. --KHGLLRYRMERGVDNWKVDCKCGTKDDDGERMLACDGCGVWHHTRCIG --KYALSHFRMEKGQENWIVDCMCGAKDDDGERMLACDTCGVWQHTRCAG --KNGLGKFRMERGTERWTVDCRCGAKDDDGERMLACDVCGVWQHTRCSG --KNGLGKFRMERGTEGWTVDCCCGAKDDDGERMLACDVCGVWQHTRCSG --NRRIVQFRMERGLENWTVDCTCGAKDDDGERMLACDVCGVWQHTRCSG --ERRFAIYRMERGVDKWTVKCSCGATDDDGERMLSCDTCHMWMHTRCAG ----VASELRYQGGFEQWTVQCMCGTGDDDGERMIACDKCGVWMHTRCVG HGIDLESKLKCQGGCDTWMVKCICRARDDDGERMISCDVCEVWQHTRCCG YGMDINSQLRYEGGSDNWKVRCECGACDDDGERMVECDICEVWQHTRCNG FGLDIGTELRYEGGFDDWTVDCKCGARDDDGERMVACDACKVWHHTLCNS CGLDLDTKLRYQGGADDWTVDCGCGAKDDDGERMVACDVCHVWQHRRCNS NGADMETELRCHGGLDLWEVRCVCGAQDDDGERMIACDACDVWHHTRCVG --IEVTKCYEDDDKKKDKRIECECGATEEDGERMVCCDICEVWQHTRCVG --INELIYECG---VNNRVVDCACGAKEDDGERMISCDICEVWQHSRCVQ KEEAAAAAVCEGSGGGERVVDCACGAVDDDGERMACCDICEAWQHTRCAG : * * : ::***** ** * * * * 5

8 INNADALPSKFLCFRCIELYSKKPKQSKKERGSSQVPK--AGFVCRGESA IDNCDEIPEKFECMRCINSYLNKSERHLKE IPDSDPVPAKFVCVGC IPDSDSVPAKFVCLRCRGSSL ISDFDDVPEKFICRKCASPRRGKGRGGGGGNGGSRMDVSAAGRCKDEIGS ISDFDRVPKRYVCKSCKLTHKPKSSGPRLMYSSAPNKRCKTGTGSFSNAA IKDNKNAPSHWICPNCEPRQEEEKLGASKKSRPAPRRREK IDDSDTLPPLFVCSNCCEEFAEQQRKVLQPKYEFPSSENVFLLESADDFF IDDADTVPQLFICSGCCDSLL IEDDEAVPSVFLCNMCYGDSLRSKKRNLSIR IKDNEAAPRMFVCCRCKNGRKKY IADSEPVPPLFLCVLCGGALMAAGPINS VQHNEEVPRIFLCQSCDQHLIPLSFLP IPNNQEMPPIFLCSRCEKEIMILPSMP IADTEDAPHVFLCSRCDNDVVSFPSFNC :.. * : * * AMGSGSNLSVTLRVG SVGGAGKFGRMATVG-- EGLLWPQIS GDQRCLGMIFPEENYLL

9 Supplemental Table 1 Complete list of genes whose expression was affected by the mutation. 1 Transcript Probe Set DEX TargetP Gene Title 2 Possible induction 3 prediction 4 function 5 young 6 old 6 young 7 old 7 At1g _at Examined S expressed protein 17.9± ± ± ±54.2 At1g _at N.D. lipase class 3 family protein 34.9± ± ± ±29.7 At1g _at Examined S cysteine proteinase, putative protease 28.1± ± ± ±134.1 At1g _at Examined S GDSL-motif lipase/hydrolase family protein 4.6± ± ± ±23.5 At1g _at Examined cytochrome P450 family protein lipid, CYP86C3 24.2± ± ± ±42.9 At1g _at N.D. S cytochrome P450, putative lipid, CYP86C4 13.0± ± ± ±19.1 At1g _at N.D. family II extracellular lipase, putative 22.5± ± ± ±104.7 At1g _at N.D. caleosin-related family protein PC 9.5± ± ± ±205.4 At1g _at Examined caleosin-related PC 3.1± ±4.3 7± ±22.2 At1g _at N.D. expressed protein 15.6± ± ± ±39.2 At1g _at N.D. expressed protein 13.5± ± ± ±7.1 At1g _at N.D. expressed protein 0.9± 55.0± ± ±91.6 At1g _at N.D. expressed protein 6.7± ± ± ±15.3 At1g _at Examined expressed protein 6.6± ± ±25.8 8±47.4 At1g _at N.D. expressed protein 15.7± ± ± ±55.0 At1g _at Examined hypothetical protein 0.9± ± ± ±5.1 At1g _at N.D. expressed protein 14.0± ± ± ±24.6 At1g _at N.D. S expressed protein 12.1± ± ± ±8.9 At1g _at N.D. S expressed protein 196.8± ± ± ±155.0 At1g _at N.D. S cytochrome P450, putative CYP705A24 7.9± ± ± ±184.2 At1g _s_at N.D. expressed protein 9.8± ± ± ±3.9 At1g

10 Transcript Probe Set DEX TargetP Gene Title 2 Possible induction 3 prediction 4 function 5 young 6 old 6 young 7 old 7 At1g _at N.D. expressed protein 26.6± ± ± ±87.6 At1g _at N.D. S pectate lyase family protein 13.5± ± ± ±112.8 At1g _at Examined cellulose synthase family protein 1.4± ± 35.6± ±8.2 At1g _at N.D. M hypothetical protein 75.2± ± ± ±15.2 At1g _at Examined S expressed protein 26.0± ± ± ±182.1 At1g _at N.D. S expressed protein 1.6± ± ± ±93.1 At1g _s_at N.D. auxin-responsive GH3 family protein 8.2± ± ± ±11.6 At4g37390 At1g _at Examined no apical meristem (NAM) family protein TF 6.2± 10.5± ± ±49.4 At1g _at N.D. caffeoyl-coa 3-O-methyltransferase, putative PP 7.1± ± ± ±195.1 At1g _at Examined S expressed protein 0.8± ± ± ±307.3 At1g _at N.D. S beta-ketoacyl-coa synthase family protein lipid 25.9± ± ±8 21.5±21.2 At1g _at Examined cytochrome P450, putative PP, CYP98A8 5.5± ± ± ±31.8 At1g _at N.D. cytochrome P450, putative PP, CYP98A9 40.7± ± ± ±15.6 At1g _at N.D. S family II extracellular lipase 4 (EXL4) PC 20.2± ± ± ±181.5 At1g _at Examined S family II extracellular lipase 5 (EXL5) PC 11.6± ± ± ±55.4 At1g _at N.D. S family II extracellular lipase 6 (EXL6) PC 3.3± 61.4 ± ± ±350.4 At1g _at Examined S glycosyl hydrolase family 1 protein / anther-specific protein ATA ± ± ± ±243.5 At1g _at N.D. cinnamoyl-coa reductase family PP 22.4± ± ± ±22.6 At2g _at Examined aspartyl protease family protein protease 4.5± ± ± ±18.1 At2g _at N.D. late embryogenesis abundant domain-containing protein / LEA 96.9± ± ± ±91.1 domain-containing protein At2g _at N.D. C late embryogenesis abundant domain-containing protein / LEA 51.2± ± ± ±

11 Transcript Probe Set DEX TargetP Gene Title 2 Possible induction 3 prediction 4 function 5 young 6 old 6 young 7 old 7 domain-containing protein At2g _at N.D. S gibberellin-responsive protein, putative 29.9± ± ± ±23.2 At2g _at Examined transferase family protein PP 8.3± ±2.1 38± ±18.3 At2g _at N.D. dehydrin family protein 2.5± ± ± ±217.4 At2g _at Examined S geranylgeranyl pyrophosphate synthase (GGPS2) (GGPS5) / GGPP PT 1± ± ± ±178.9 synthetase / farnesyltranstransferase At2g _at Examined C methyltransferase-related 40.2± ± ± ±41.8 At3g _s_at N.D. phosphoesterase family protein 6.6± ± ± ±30.2 At3g03530 At3g _at N.D. glutamate dehydrogenase, putative 21.9± ± ± ±20.8 At3g _at Examined S expressed protein 1± ± ± ±33.4 At3g _at S glycosyl hydrolase family 17 protein 20.3± ± 235.3± ±18.2 At3g _at Examined S xyloglucan:xyloglucosyl transferase, putative / xyloglucan 19.1± ± ± ±173.0 endotransglycosylase, putative / endo-xyloglucan transferase, putative At3g _at N.D. cytochrome P450, putative lipid, CYP86C2 64.6± ± ± ±166.7 At3g _at Examined S lipid transfer protein, putative lipid 22.1± ± ± ±211.5 At3g _at Examined beta-ketoacyl-coa synthase family protein lipid 2± ± ± ±31.8 At3g _at N.D. S purple acid phosphatase (PAP21) 0.9± ± ± ±25.0 At3g _at N.D. eukaryotic rpb5 RNA polymerase subunit family protein 4.4± ± ± ±3.4 At3g _at N.D. glyoxal oxidase-related 44.2± ±4.8 21± ±19.4 At3g _at N.D. OTU-like cysteine protease family protein protease 108.8± ± ± ±92.1 At4g _at N.D. sugar transporter family protein 21.6± ± ± ±8.5 At4g _at N.D. S protease inhibitor/seed storage/lipid transfer protein (LTP) family protein 67.2± ± ± ±66.2 3

12 Transcript Probe Set DEX TargetP Gene Title 2 Possible induction 3 prediction 4 function 5 young 6 old 6 young 7 old 7 At4g _s_at Examined S pectate lyase family protein 0.4± ± ± ±11.8 At4g22090 At4g _at N.D. M UbiA prenyltransferase family protein PT 49.2± ± ± ±23.0 At4g _at N.D. ABC transporter family protein 36.4± ± ± ±16.2 At4g _at N.D. multi-copper oxidase type I family protein 56.8± ±3.5 17± ±11.6 At4g _at Examined lipid transfer protein, putative lipid 30.4± ± ± ±7 At4g _at N.D. S ammonium transporter, putative 1.7± ± ± ±12.3 At4g _at N.D. transferase family protein 22.1± ± ± ±12.8 At4g _at N.D. glycine-rich protein 23.4± ± ± ±196.9 At5g _at N.D. expressed protein 5.6± 3.1± ± ±4.6 At5g _at N.D. S glycine-rich protein (GRP14) PC 7.4± ± ± ±81.5 At5g _at N.D. S glycine-rich protein (GRP18) PC 9.9± ± ± ±105.5 At5g _at Examined glycine-rich protein (GRP17) PC 10.3± ± ± ±455.9 At5g _at N.D. S glycine-rich protein (GRP16) PC 26.4± ± ± ±143.7 At5g _at N.D. S glycine-rich protein (GRP19) PC 25.7± ± ± ±889.6 At5g _at N.D. glycine-rich protein (GRP20) PC 8.1± ± ± ±519.3 At5g _at N.D. C expressed protein 100.6± ± ± ±58.8 At5g _at Examined auxin-responsive GH3 family protein 9.0± ± ± ±34.5 At5g _at N.D. esterase/lipase/thioesterase family protein 18.9± ± ± ±5.1 At5g _at N.D. NADP-dependent oxidoreductase, putative 16.4± ± ± ±41.8 At5g _at Examined S FAD-binding domain-containing protein 6.3± ± ± ±56.2 At5g _at N.D. S expressed protein 28.3± ± ± ±12.2 At5g _at N.D. S expressed protein 35.4± ± ± ±79.7 4

13 Transcript Probe Set DEX TargetP Gene Title 2 Possible induction 3 prediction 4 function 5 young 6 old 6 young 7 old 7 At5g _at N.D. beta-ketoacyl-coa synthase family protein lipid 14.2± ± 112.8± ±5.6 At5g _at N.D. glycosyltransferase family protein 6.1± ± ± ±4.1 At5g _at Examined senescence-associated protein-related 1.4± ± ±8 80.9±56.4 At5g60500 At5g _s_at Examined undecaprenyl pyrophosphate synthetase family protein / UPP synthetase family protein PT 1.3± ± ± ±12.9 At5g _at N.D. S expressed protein 8.3± ± ± ±209.8 At5g _at Examined myb family transcription factor (MYB99) TF 9.2± ± ± ±7.9 At5g _at N.D. short-chain dehydrogenase/reductase (SDR) family protein 19.6± ± ± ± We have used available microarray data (Experiment ID: NASCARRAYS-23) at After eliminating genes with signal values less than 15.0 in young, we have selected genes with more than 5-fold higher expression in young than in old. Among them, we have further selected genes with more than 3-fold higher expression in young than in young. In total, we have isolated 95 genes. 2 These columns are the same as the original TIGR ATH database. 3 Examined, 32 genes out of 95 were examined for DEX inducibility by using the DEX-induction system as shown in Fig. 6C and Supplemental Figure 1. N.D., not determined. 4 Potential subcellular localization signal was predicted by using TargetP program (Emanuelsson et al., 2000; Localization to secretory pathway (S), chloroplast (C) and mitochondrion (M) with prediction reliability class 1-3 is indicated. 5 Possible function of a gene product involved in lipid biosynthesis and transfer (lipid), phenylpropanoid biosynthesis (PP), pollen coat protein (PC), cytochrome P450 family (CYP), protease, transcription factor (TF) or prenyltransferase (PT). 6 These columns show average of signal values (± standard deviations) from 3 independent hybridization slides done by NASCArray. 7 These columns show average of signal values (± standard deviations) from 5 independent hybridization slides done by NASCArray. 5

14 Supplemental Table 2 Ct values in Figure 6 and Supplemental Figure 1 Ct values in Figure 6C Lane At1g06260 At1g13140 At1g23250 At1g61110 At1g74540 At1g75920 At2g03200 At2g19070 At2g23800 At3g51590 At3g52160 At4g28395 At5g07530 At5g60500/ At5g60510 At5g ± ± ± ± ± ±2 8.02± ± ± ± ± ± ± ± ± ± ± ±6 3.37± ± ± ± ± ± ± ±8-0.15± ± ± ± ± ± ± ± ± ± ± ± ± ± ±4 6.63± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.55 Ct±Standard deviation Ct values in Figure 6D X-axis At2g19070 At1g61110 At3g51590 At3g52160 At5g62320 At4g hr (Mock) 8.85± ± ± ± ± ± hr (Mock) 9.76± ±2 9.44±0 9.47± ± ± hr (Mock) 9.93± ± ± ± ± ± hr (Mock) 10.58± ± ± ± ± ± hr (DEX) 9.09± ± ± ± ± ± hr (DEX) 11.28± ± ± ± ± ±0 12 hr (DEX) 9.02± ± ± ± ± ± hr (DEX) 5.09± ± ± ± ± ±0.25 Ct±Standard deviation 6

15 Ct values in Figure 6E Lane At4g17500 At3g16720 At5g ± ± ± ±8 7.80±9 8.12± ±7 1.52± ± ± ± ±0.45 Ct±Standard deviation Ct values in Supplemental Figure 1 Lane At1g02813 At1g06990 At1g23590 At1g23610 At1g32180 At1g47980 At1g68875 At1g75940 At2g41040 At3g20450 At3g25050 At4g22080 /At4g22090 At5g13380 At5g44400 At5g ± ± ± ± ± ± ± ± ± ±0.17 3± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±7 8.15± ± ± ±0.70 Undetermined 12.41± ± ± ± ± ± ± ±0 Undetermined 8.59± ± ± ±7 8.14±0.34 Undetermined 12.84± ± ± ± ± ± ±0.22 Undetermined Undetermined 8.43± ± ± ± ± ± ± ± ±8 8.12± ± ± ± ±0.36 Undetermined 8.59± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.59 Ct±Standard deviation 7

16 Supplemental Table 3 Primers used in the real-time PCR experiment. Primer name Sequence (from 5 to 3 ) Application omc1533 GGT AAC ATT GTG CTC AGT GGT GG Forward primer for actin gene omc1534 AAC GAC CTT AAT CTT CAT GCT GC Reverse primer for actin gene omc2291 AGA GGA CGG GTC ATA AGG TTG A Forward primer for At1g02813 gene omc2292 GGC TGT CCA CGA AAT ACT TGC T Reverse primer for At1g02813 gene omc2293 TCA AGG AAA ATG CGG AGG TT Forward primer for At1g06260 gene omc2294 TCC GCC ACT ACA GCC TTT GT Reverse primer for At1g06260 gene omc2305 GCA GAA CGA GAG ACG ATG CA Forward primer for At1g06990 gene omc2306 GGC CAT GTC GAA CAA TGC T Reverse primer for At1g06990 gene omc2285 AAC CAG GTT CGT GGA GCA TT Forward primer for At1g13140 gene omc2286 GGC GAT GCA GAT GTT GTC AA Reverse primer for At1g13140 gene omc2283 GTG TCC TTC TTT GAC CGA AAC G Forward primer for At1g23250 gene omc2284 TTG AAC CGG ACG AGT TTT CTG Reverse primer for At1g23250 gene omc2255 ACT CGT CAG TGA CAA GTC TTG G Forward primer for At1g23590 gene omc2256 ATT TAT CGG CAT ACC TCC AGA CC Reverse primer for At1g23590 gene omc2257 AGA ATT GCA TCG ATG CCT TCT AC Forward primer for At1g23610 gene omc2258 CAA GTG GTA ACA TCA CTT TTG TGG Reverse primer for At1g23610 gene omc2321 CGT GAT CTC GTG CTG GTA TGA Forward primer for At1g32180 gene omc2322 GAA TGC GTC TGG CTC TGT CA Reverse primer for At1g32180 gene omc2299 GAT CAG GCG ACG ACA AAC TGT Forward primer for At1g47980 gene omc2300 TTG GCC CTC CCA TTG TTA GA Reverse primer for At1g47980 gene omc2259 AAT GGA AGG CAT GCT CTC AAA Forward primer for At1g61110 gene omc2260 TCG GTA TTG ACC ACA CAA GGA A Reverse primer for At1g61110 gene omc2297 TGT TTT CCC GGT CTC TGC AT Forward primer for At1g68875 gene omc2298 CAC ATG AGC CAC CAT AAC AAG TC Reverse primer for At1g68875 gene omc2295 CAT TCT TTC TCG GAG TGG TCA GA Forward primer for At1g74540 gene omc2296 TTG CGA TCC TGT GTC GGT TA Reverse primer for At1g74540 gene omc2289 TCA ATA GAA GCT GTC CGG AAT GA Forward primer for At1g75920 gene omc2290 CGG TCG GTA TTT TTG AGT CGA A Reverse primer for At1g75920 gene omc2287 ACT TAT CGC CGC CAA TGA GT Forward primer for At1g75940 gene omc2288 CCA CAT GCT TGG ACC TCT ACA TC Reverse primer for At1g75940 gene omc2253 TCA TGG AGG AAG CGG TGA GT Forward primer for At2g03200 gene omc2254 GTC AAA AAT CGG AGT TGG TTG ATC Reverse primer for At2g03200 gene omc2247 CAA CGT GGA GCG GTA GGT TT Forward primer for At2g19070 gene omc2248 ACT AGC ACA CGG CTC AAC GA Reverse primer for At2g19070 gene 8

17 Primer name Sequence (from 5 to 3 ) Application omc2249 GCC AAT ACT CTG CCT TGC TTC T Forward primer for At2g23800 gene omc2250 CTT TCC ACG ACG CAA ATC G Reverse primer for At2g23800 gene omc2251 TGC GCA GTC TGG CAA ATA CTC Forward primer for At2g41040 gene omc2252 GAA AGG GAG GCG AGA AAC G Reverse primer for At2g41040 gene ti0043 CGT TCA GAC CCG AAG ACA ACA Forward primer for At3g16720 gene ti0044 CGA GCA CGG AGG AGA TAC CA Reverse primer for At3g16720 gene omc2301 AAG GTC GAG CAC CAC TGG TT Forward primer for At3g20450 gene omc2302 CGA TTG GCA AAC ATG ACT TGA Reverse primer for At3g20450 gene omc2303 ACG CCT ACG GAT CAG GTC TCT Forward primer for At3g25050 gene omc2304 ACC GGT TTT CCG TTG TTG TTC Reverse primer for At3g25050 gene omc2309 CAT GGC ATC CCC AAC AGA GT Forward primer for At3g51590 gene omc2310 CGG TGT GGT CTG AGC CAA TT Reverse primer for At3g51590 gene omc2263 GTG CTG GAC CAA TCA GGG ATA Forward primer for At3g52160 gene omc2264 TGA TTT TGG TCG CTG CAA GA Reverse primer for At3g52160 gene ti0033 CGG CGG ATT CTC AAT CTG AT Forward primer for At4g17500 gene ti0034 TTC GTC CGT ACA CCG GTT TAA Reverse primer for At4g17500 gene omc2307 AAG TCT GAC TGG GCT GCC AAT Forward primer for At4g22080 gene omc2308 CGT ACC GCA AAG AAC CTG GTT Reverse primer for At4g22080 gene omc2261 GGA TCG AGG AAT AGG CAG GAT Forward primer for At4g28395 gene omc2262 CGG AGA TGG GAA AGC TGA GA Reverse primer for At4g28395 gene omc2311 CTG CCG CAG CAT TAG TCG TA Forward primer for At5g07530 gene omc2312 ACA AGC CAC ATG ATG AGA CCA A Reverse primer for At5g07530 gene omc2313 CCT CAC CGG TCA ACT CGA TAA Forward primer for At5g13380 gene omc2314 CGT GGT GGC CAA GAG AAG AC Reverse primer for At5g13380 gene omc2315 GGT TCA ATC TGG TGC CAC TGT Forward primer for At5g44400 gene omc2316 ACC ATA TTT CCG CAT CAT GGA Reverse primer for At5g44400 gene omc2317 TGG CGT ATG CCG GAG TAA AT Forward primer for At5g57810 gene omc2318 CCG TAA ATG AGG TCG TTG CA Reverse primer for At5g57810 gene omc2319 CCA CGC GTT TGG TAT GGA TAA Forward primer for At5g60500 gene omc2320 ATC CAA ATC ACC GGC AAA GA Reverse primer for At5g60500 gene omc2265 GCT GGA GAG ACG TGC CAA AA Forward primer for At5g62320 gene omc2266 CCA AGG CGA GCA TGA AGA TC Reverse primer for At5g62320 gene 9