THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY

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1 THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO: GDP 020 MOD: 1st Issue Page: 1 of 5 Procedure Type: General Discipline Procedure Title: Handling Material for Autoclaving 1. Risk Assessment: This risk assessment has been prepared using the National Safety Council of Australia Risk Score Calculator and risks have been determined to be as stated. This Risk Assessment is to be used as a general guide and as such, cannot accommodate all the varying factors that may be encountered when using this procedure. Therefore, personnel are requested to conduct their own Risk Assessment before using this procedure to include any extra hazards introduced by the task performed. TASK PERFORMED Preparing materials for autoclaving and transporting to the autoclave on Level 2 Life Sciences. 1. Handling infectious materials 2. Transporting infectious materials HAZARDS RISK ASSESSMENT 1. The risk of handling infectious materials and transporting infectious materials is low provided that instructions given in the Risk Control are followed RISK CONTROL 1. Wear gloves and safety glasses when handling infectious materials. 2. Place all infectious waste in a waste container, either a bag or a sharps bin which has been designated as suitable for autoclaving. 3. Ensure that all residual chemicals have been removed from tubes before placing them in the waste containers. 4. Do not leave bleach in any item which is to be autoclaved. 5. When transporting infectious materials out of the laboratory use a leak-proof secondary container with a lid so that any spill can be contained and disinfected. 2. Purpose: 2.1. To describe the manner in which materials should be prepared for autoclaving and transported to the autoclave on Level 2 Life Sciences. 3. Equipment: 3.1. Bucket and Lid

2 THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO: GDP 020 MOD: 1st Issue Page: 2 of 5 Procedure Type: General Discipline Procedure Title: Handling Material for Autoclaving litre Container and Lid 4. Materials: 4.1. Autoclave bags litre Contaminated Waste bins / Sharps bins litre Contaminated Waste bins / Sharps bins 4.4. Liquid Containers 5. Set Up: 5.1. Any person dealing with Autoclavable Waste should have read the GDP 007 Microbiological Safety Procedure Read the Waste Disposal section 5.14 of the General Laboratory Safety Procedure GDP Tasks and Methods: 6.1. Infectious and Genetically Modified Solid Waste Infectious or genetically modified solid waste should be collected into bins which have been double lined with clear plastic autoclave bags When autoclave bags are two-thirds full they should be removed from the bin and sealed with either a wire tie or tape Bags must be clearly identified with the name of the generator and a contact phone number Bags should then be placed into the 60 litre plastic container and its lid should be put in place to seal the container Infectious and Genetically Modified Liquid Waste Infectious or genetically modified liquid waste (such as that in vacuum traps) may be autoclaved to sterilise before disposal, however if it has been treated with a chemical disinfectant such as bleach, then it MUST NOT be sent for autoclaving Infectious or genetically modified liquid waste containers may be placed in either the bucket or 60 litre container with its respective lid in place, for transport to the autoclave Bottles must be clearly identified with the Contents, name of the generator and a contact phone number Infectious and Genetically Modified Sharps

3 THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO: GDP 020 MOD: 1st Issue Page: 3 of 5 Procedure Type: General Discipline Procedure Title: Handling Material for Autoclaving Any infectious or genetically modified sharp must be placed into a yellow sharps container which has been designated as suitable for autoclaving Infectious or genetically modified sharps may include such items as scalpels, needles and broken glass. Other less obvious infectious items such as pipette tips or 10 ml disposable pipettes may also be treated as sharps if there is the possibility that they would puncture an autoclave bag Sharps containers should be sealed with their own accompanying lid before being placed in either the bucket or container with its respective lid in place, for transport to the autoclave Containers must be clearly identified with the name of the generator and a contact phone number Non-infectious Material for autoclaving Non-infectious material may also be autoclaved to ensure sterile conditions Material can be prepared either by placement in an autoclave bag which is subsequently sealed with autoclave tape, or by placing autoclave tape over the lid of the item The autoclave tape will develop black stripes if it has reached 100 o C during the autoclave process There is no requirement for secondary containment of non-infectious items for autoclaving Liquids must be clearly labelled with the Contents, Contact name and phone number Dry goods must have the Contact name and phone number Transport to Autoclave The autoclave which Medical Biochemistry uses, is situated on Level 2 of the Life Sciences Building, room LS2.18 Corridor 3. It is operated by the Teaching Lab staff Items for autoclaving should be placed on the Medical Biochemistry trolley and transported on the trolley to the autoclave See Attachment 9.1 for the recommended route to follow to the autoclave Transfer of Items to Secondary Containment in the Autoclave room There are three large white plastic containers on the floor of the autoclave room.

4 THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO: GDP 020 MOD: 1st Issue Page: 4 of 5 Procedure Type: General Discipline Procedure Title: Handling Material for Autoclaving All bagged infectious material should be placed into these containers when it is brought to the autoclave room All non-bagged infectious material should be placed on the bench above the container, on the left hand side All non-infectious material for sterilisation should be placed on the benches above the containers, on the right hand side Staff who have been trained in the operation of the autoclave will proceed with further handling of material brought to the autoclave room Recovery of material after autoclaving After it has been autoclaved, bagged waste will be placed into the contaminated waste wheelie bin in the autoclave room by the staff operating the autoclave All other material should be picked up as soon as possible after autoclaving. Check Attachment 9.2 for autoclave scheduling Carry material back from the autoclave in the same manner as it was taken. (ie. in a secondary container and following the route in reverse) After autoclaving liquid waste may be disposed of as is appropriate for the particular reagent involved. Refer to the MSDS for details. Agar should be left to solidify and be discarded into a contaminated waste bag. 7. Maintenance: 7.1. Check the stocks of autoclave bags, sharps containers and autoclave tape when obtaining these items, order if required. 8. Shutdown: 8.1. Return the trolley, 60 litre container and bucket to Level 3 Life Sciences If any material leaks into the secondary containers, disinfect with 5% Bleach solution Check the integrity of the secondary containers. If there are any cracks report this to the Laboratory Manager so that they can be replaced. 9. Attachments 9.1. Map of recommended route for transportation of infectious waste to autoclave on Level 2 Life Sciences Guidelines for the use of Autoclaves 9.3. Laboratory Waste Disposal (LSB)

5 THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO: GDP 020 MOD: 1st Issue Page: 5 of 5 Procedure Type: General Discipline Procedure Title: Handling Material for Autoclaving 10. References: Microbiological Safety Procedure GDP General Laboratory Safety Procedure GDP Change History: 11.1 Issue Number: 1st Issue 11.2 Issue Number: Date Issued: Date Issued: Reason for Change:

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8 Attachment 9.2: USE OF AUTOCLAVES IN LIFE SCIENCES BUILDING In order to provide sterilisation of equipment, media etc., in a cost and labour effective manner and to provide a regular autoclave service, the following is proposed for your consideration and comments. GUIDELINES FOR THE USE OF THE AUTOCLAVES GENERAL INFORMATION: The autoclaves will be operated by Stephanie van Horck who works on Level 2, rm 2-21, phone 17824, e-mal bishav@alinga.newcastle.edu.au One or two people from each user group should be trained to use the autoclaves so that if necessary, on weekends or public holidays someone will be available for unexpected runs. Please advise Steph if you need to train a new person or you want a refresher. Stephanie should be the only person to use the autoclaves except for weekends or public holidays when she might not be around. Stephanie will log all runs and check sterility with test strips in each run. Class materials will be given priority as they have to run to a timetable. The autoclaves will be blown down at 4:30pm daily. CONTAMINATED WASTE All staff are responsible for the disposal of their own laboratory waste. All waste bags should be delivered to the autoclave room and placed in the tub under the bench, but must be clearly identified with the name of the generator and a contact phone number. Once autoclaved, bagged waste will be discarded in yellow bin in autoclave room. _Bottled waste should be delivered to the autoclave room clearly labelled as waste and left in the area designated for it. Contaminated waste will be autocloaved DAILY. ALL bags should be DOUBLE-BAGGED. WET AND DRY CLEAN GOODS There will be a Liquids run at 10am, 12noon and 2pm DAILY. All goods brought down other than for these times, will be autoclaved as space becomes available. Bear in mind that media, particularly rich media will be contaminated if left on the bench for several hours before autoclaving. Note tha 3:30pm is the last opportunity to start a run. Media will always be autoclaved on the day of delivery unless after 3:30pm. Any agars that need to be kept liquid can be put into a 50 C waterbath or Steph can ring you, you just need to leave these details on your container. All goods must be picked up as soon as possible, but at least on the next day. A normal run takes approx 1 hour. Dry goods will be autoclaved as soon as there is a reasonable load and may be held over to the next day if insufficient items are waiting to make an economical run. ALL items to be autoclaved MUST be identifiable:- Liquids MUST be labelled with the CONTENTS, CONTACT NAME AND PHONE NUMBER. Dry goods MUST have the NAME AND CONTACT PHONE NUMBER.

9 The above are guidelines and are flexible to allow for all users requirements. Please speak to Steph about specific requirements. Attachment 9.3: LABORATORY WASTE DISPOSAL (LSB) GENERAL WASTE, such as packing, paper and paper towel is placed into the grey waste bags provided. Large boxes should be placed in the corridor. Noncontaminated glass (broken glass, washed bottles) should be packed in a clearly labeled (Caution: glass) box and placed in the corridor. The cleaners remove all general waste daily. LABORATORY-GENERATED WASTE (excluding categories mentioned below) is placed into yellow contaminated waste bags (medical store codes B076). When ¾ full, seal with tape and place in the yellow whiz bin in your area. SHARPS are placed in sharps bins (medical store codes C021/B670) after use. Only fill up to the line shown on the bin. When the bin is full, seal and place in the yellow contaminated waste whiz bin in your area. NB All full yellow whiz bins are to be locked and taken down to level 0 for collection by Cleanaway (Pick-up time is Monday morning). CELL CULTURE LIQUID WASTE is autoclaved or diluted in bleach (medical store code A030) for a minimum of 30 minutes before being washed down the sink with water. LIQUID MICROBIAL/GENETICALLY MODIFIED WASTE is autoclaved down in the autoclaves on level 2 (operated by teaching lab staff), before disposal down the sink with water. SOLID MICROBIOLOGICAL/GENETICALLY MODIFIED WASTE is placed into autoclavable contaminated waste bags (medical store code B075) or sealed in autoclavable vessels and autoclaved down in the autoclaves on level 2 (operated by teaching lab staff). They are then placed in the yellow contaminated waste whiz bin for disposal. ANIMAL CARCASSES, TISSUE etc are placed into black body bags, (provided by the animal house) and transported to the freezer in the animal house area, MSB level 2. You will need a key/animal house staff to unlock the door to the corridor. Fill in the animal house logbook provided. RADIOACTIVE WASTE all staff working with radioactive material, receive instruction on safe use, monitoring and disposal from the Radiation Officer

10 (Lynn Herd) who is responsible for ensuring the safe operation of the Hot Lab (LSB 318). Radioactive Waste bags (B175) and plastic liners (B176) are ordered through the Medical Store. CHEMICAL WASTE- refer to MSDS. See the Resource Manager to organise the pickup of hazardous waste. No hazardous material should be poured down the sink. MM 3/2001