Shifts in Diversity and Function of Sediment Bacterial Community in the Hengshi River upon Acid Mine Drainage Pollution

Transcription

1 Shifts in Diversity and Function of Sediment Bacterial Community in the Hengshi River upon Acid Mine Drainage Pollution Song Tang SETAC Orlando Nov 8, 2016

2 Mining Activities Mining The extraction of valuable minerals or other geological materials from the earth, from an ore body, vein or (coal) seam. Mining of metals has been a human activity since pre-historic times. World Mining Map In 2015, the global gold market had a value of $131.5 billion, an increase of 57% since Harmful Environmental Effects of Mining Contamination of Soil, Groundwater, Surface Water, Crop and Plant Acid Mine Drainage (AMD)

3 AMD Formation Pyrite (FeS 2 ) AMD 4FeS H 2 O + 15 O 2 4Fe(OH) H SO 4 2-

4 Features of AMD

5 Microbial Communities in AMD Systems Huang LN et al., Trends Microbiol Jul;24(7):

6 AMD-contaminated River Few studies systematically explore the diversity and function of microorganisms along the AMD pollution gradients in a open lotic ecosystem, such as river. Dabaoshan Mine Guangdong Province Built in 1958 Full-scale in 1970s Mining activity

7 Sampling Sites ph Acidic Neutral

8 Objectives I. Characterize the spatial dynamics of the bacterial community structure and composition along the AMDpolluted Hengshi River II. Explore the relationships between bacterial structure and different geophysicochemical variables in Hengshi River I. Study the functions of bacterial communities along the AMD pollution gradients

9 Experimental Workflow 27 Samples 4 Samples Barcode PCR Ion Torrent OUT Clustering Sequence data analysis Extract edna Sediment Hiseq-2500 Water Sampling Sediment Physicochemical analysis Packages: phyloseq, ggplot2, edger, vegan, etc. Operational Taxonomic Unit (OTU) table

10 Physicochemical Results Aq. -ph Aq. -Cu Aq. Zn Aq. -Cd Aq. -Pb Sd. -ph Sd. -Cond. Sd. -Cu Sd. -As Sd. -Pb

11 Bacterial Communities

12 Bacterial Abundance Hengshi vs. Reference River Hengshi River Reference River

13 Alpha Diversity

14 Beta Diversity

15 Canonical Correspondence Analysis (CCA) CCA

16 Comparative Metagenomics ph Acidic Neutral

17 Comparative Metagenomics

18 Metal Resistance Genes

19 Summary I. Geophysicochemical parameters of water and sediments significantly differed among samples collected from environments that differed in their extent of contamination with heavy metals. II. III. IV. 16S rrna sequencing results revealed that alpha diversity of bacterial community gradually increased along the river with decreasing contaminant levels, which was supported by beta diversity. At the phyla level, Proteobacteria was significantly higher in the Hengshi River, while Firmicutes and Actinobacteria were significantly higher in the Reference River. ph is the major geochemical determinant of the bacterial community diversity. V. Furthermore, comparative metagenomic analysis showed diverse metabolic capacities for heavy metal-detoxification was found along the pollution gradient of Hengshi River. VI. Our findings offer a deep insight into the patterns of microbial community structure and function along the river system contaminated by AMD.

20 Acknowledgements Prof. Markus Hecker Prof. John P. Giesy Dr. Hui Peng Prof. Mao Wang Xiaohui Zhang Yuwei Xie Prof. Hongling Liu Prof. Hongxia Yu Prof. Weimin Sun

21 Key References Caporaso, J. G., J. Kuczynski, J. Stombaugh, K. Bittinger, F. D. Bushman, E. K. Costello, N. Fierer, A. G. Pena, J. K. Goodrich and J. I. Gordon (2010). "QIIME allows analysis of high-throughput community sequencing data." Nature methods 7(5): Huang, L. N., W. H. Zhou, K. B. Hallberg, C. Y. Wan, J. Li and W. S. Shu (2011). "Spatial and Temporal Analysis of the Microbial Community in the Tailings of a Pb-Zn Mine Generating Acidic Drainage." Applied and Environmental Microbiology 77(15): Kuang, J.-L., L.-N. Huang, L.-X. Chen, Z.-S. Hua, S.-J. Li, M. Hu, J.-T. Li and W.-S. Shu (2013). "Contemporary environmental variation determines microbial diversity patterns in acid mine drainage." ISME J 7(5): Larson, C. (2014). "China gets serious about its pollutant-laden soil." Science 343(6178): Wang, M., H. Song, W.-Q. Chen, C. Lu, Q. Hu, Z. Ren, Y. Yang, Y. Xu, A. Zhong and W. Ling (2011). "Cancer mortality in a Chinese population surrounding a multi-metal sulphide mine in Guangdong province: an ecologic study." BMC Public Health 11(1): 1. Wang, M., Y. Xu, S. Pan, J. Zhang, A. Zhong, H. Song and W. Ling (2011). "Long-term heavy metal pollution and mortality in a Chinese population: an ecologic study." Biol Trace Elem Res 142(3): Huang, L. N., J. L. Kuang and W. S. Shu (2016). "Microbial Ecology and Evolution in the Acid Mine Drainage Model System." Trends in Microbiology 24(7): Johnson, D. B. and K. B. Hallberg (2003). "The microbiology of acidic mine waters." Research in Microbiology 154(7): Johnson, D. B. and K. B. Hallberg (2005). "Acid mine drainage remediation options: a review." Science of the total environment 338(1): 3-14.

22 Thanks! TP223