AGENDA AND ABSTRACTS. An Aquafeed.com conference sponsored by:

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1 AGENDA AND ABSTRACTS An Aquafeed.com conference sponsored by: And supported by the Thai Department of Fisheries, in association with Victam International. MARCH 27, 2018, BITEC, BANGKOK, THAILAND

2 AGENDA 09:00 09:30 10:00 Welcome Dr. Juadee Pongmaneerat, Thai Dept of Fisheries, Thailand; Meredith Brooks, Associate Editor, Aquafeed.com LLC, Hawaii, USA Extruded Aquafeed Quality Management; Relationship Between Technology and Extruded Aquafeed Quality Thomas Ellegaard Mohr Aqua Feed Production How Extrusion Reliability Affects Factory Performance Rob Strathman 10:30 COFFEE BREAK 11:00 11:30 12:00 Optimizing Design and Operation of the Pre-drying Product Transport Process Anders Fjeldbo Haubjerg, Sr., PhD. Advancements in Micro Aquafeed Extrusion Ramesh Gangatharan, PhD. Mycotoxin Challenge in Aquaculture Feeds Maarten Jay van Schoonhoven 12:30 LUNCH 13:30 14:00 14:30 Balancing Nutrient Levels Through the Application of Functional Additives: Survey of Nutrient Levels in Commercial Shrimp Feeds in India Peter Coutteau, PhD. Comparing Novel Single Cell Protein Technologies For Economical Fishmeal Replacement David Tze Insects: The Missing Link in Aquaculture Tarique Arsiwalla 15:00 COFFEE BREAK 15:30 16:00 16:30 Aquafeed Probiotics - Challenges and Opportunities Benedict Standen PhD. Nucleotides and Bioactive Peptides: Boosting the Functionalities of Aquafeeds Francisco González, PhD. Immunity - The Next Frontier in Aquaculture Nutrition Kabir Chowdhury PhD.

3 EXTRUDED AQUAFEED QUALITY MANAGEMENT RELATIONSHIP BETWEEN TECHNOLOGY AND EXTRUDED AQUAFEED QUALITY Thomas Ellegaard Mohr, Global Application & Sales Manager, ANDRITZ Feed & Biofuel, Denmark One of the keys to successful fish farming lies in correctly adapted feed formulas: high quality ingredients, controlled homogenous content and optimum physical feed pellet properties. In order to keep pace with these increasing demands, industrially produced feed is undergoing a continuous quality improvement process. The different types of feed are subject to different demands, for example, the feed s functionality on fish farms in terms of or sinkability and the pellets durability to assist mechanical handling without generating fines. As the increased demand for feed also influences the availability of "classic" raw material, this gives new challenges to ensure that the extrusion technologies and process control are developed to adapt accordingly. AQUAFEED PRODUCTION HOW EXTRUSION RELIABILITY AFFECTS FACTORY PERFORMANCE Rob Strathman, Vice President, Famsun-USA Design & Engineering, USA In aquafeed production there exist many performance indicators for evaluating the corresponding factory performance. Thus, understanding how these KPIs define, monitor, and measure the overall factory efficiency can play an important role in equipment, process, and factory design. Additionally, overall factory performance is hindered by inflexible and unreliable hardware and software used in the extrusion systems of today. By identifying these obstacles, we can redesign the systems of the future, allowing significant improvements in factory performance to be realized. The need to find efficiency in every aspect of production has driven other manufacturing industries to dramatically change course. For instance, the simple assembly line of the auto industry of old, is all but gone. The onset of new technologies, such as Robotics, 3-D Printing, IIOT, AI, Big Data and Big Analytics have propelled the creation of Digital Factories. Like these other industries, the future of aquafeed production will be digital. OPTIMIZING DESIGN AND OPERATION OF THE PRE-DRYING PRODUCT TRANSPORT PROCESS Anders Fjeldbo Haubjerg, Sr., PhD., Process Engineer, Graintec A/S; Assistant Professor, University of Southern Denmark, Denmark Recent research by the author on the drying of extruded feed, particularly as related to technical product quality, has identified a connection between drying parameters and mechanical durability. It has been demonstrated that the initial phase of the drying is important to avoid early and spatially uneven glass transition following evaporative cooling. It follows that utilization of high air velocity, high temperature and high humidity are recommended. Extruded feed products start to flash off and dry immediately when exiting the extruder, hence focus needs to be put on the design

4 and operation of the different transport mechanisms immediately upstream of the dryer. Validated model simulations visualize how many existing airlifts and conveyor belts could damage the pellets when transported to the dryer. Different process design options for new as well as existing production lines, are presented and compared in terms of energy efficiency, capital and operational expenditure, impact on mechanical durability, flexibility and hygienic design. It follows that the airlift using heated air is superior for durability of the product, for existing production lines. Energy efficiency can optionally be optimized by invoking recirculation of the transport air. For new production facilities, process designs facilitating stacked dryer and extruder layout are superior. ADVANCEMENTS IN MICRO AQUAFEED EXTRUSION Ramesh Gangatharan, PhD.,Technical Sales Advisor, Wenger Manufacturing Inc., USA Traditionally aquaculture professionals have given more importance to hatchery feeds as they deal with highly sensitive larval /fry and post-larval stages of fish and shrimp. With the growing intensification of aquaculture systems and moving indoors, there has arisen the need for feeding specialty diets for the nursery stages as well. To accomplish this, they have to move over from standard pelleted and crumbled feeds to high quality complete extruded diets. Micro aquafeeds can be classified as those feeds which fall in the size range of 0.5mm to 1.2mm dia. To make these feeds, a series of meticulous steps must be followed right from the selection of high quality ingredients to specific grinding, sifting, mixing, precision twin screw extrusion and optimal drying process. Advanced computer controls, variable frequency drives, unique die designs and specially selected dryer screens all contribute to a smooth process at high production capacities. In-line moisture and density monitoring systems can be fixed at critical points to monitor the production process so as to minimize production of out of spec product and to have a final product that is uniform in size, shape and density. Feeding of this micro extruded aquafeed will help the aquatic animals in the nursery phase attain the optimal nutrition, good health and better growth performance with the best FCR. MYCOTOXIN CHALLENGE IN AQUACULTURE FEEDS Maarten Jay van Schoonhoven, Aqua Care manager, Olmix group, Netherlands There is a trend to use increasing amounts of plant ingredients in aquaculture feeds. With it, the risk of mycotoxin contamination also increases. These toxins are produced as secondary metabolites by fungi and can have serious detrimental effects on fish and shrimp, ranging from mortalities in some cases, to economic impacts such as reduced growth, reduced immunity, decreased feed efficiency. They can be caused by a single mycotoxin, or as is more common, by more mycotoxins working together synergistically causing even more damage. Research into the effects of mycotoxins in aquaculture is Increasing but more research is needed to accurately describe the negative impacts of mycotoxin in aquaculture production. Clinical signs of mycotoxicosis can easily be overlooked or misinterpreted, most often leading to a wrong approach in dealing with the negative effects. Knowledge and awareness of the role of mycotoxins in aquaculture production is necessary, just

5 as adequate strategies with a wide spectrum are necessary to deal with mycotoxin risk management. BALANCING NUTRIENT LEVELS THROUGH THE APPLICATION OF FUNCTIONAL ADDITIVES: SURVEY OF NUTRIENT LEVELS IN COMMERCIAL SHRIMP FEEDS IN INDIA Alexander van Halteren and *Peter Coutteau, PhD., Business Unit Manager Aquaculture, Nutriad International NV, Belgium The optimal nutrient profile of a shrimp feed depends on many factors; as a result, research in optimizing feed formulations under practical conditions continues to be a major objective for feed producers. We can also expect a wide variation in nutritional specifications among commercial shrimp feeds as composition may depend on the target market. Increasing cost and fluctuating availability of raw materials in combination with an increasingly competitive market demands a creative mind from the shrimp feed formulator. The nutritional strategy is key to maintain or gain market share. The present study investigated the different nutritional strategies in commercial shrimp feeds during 2016, when the number of shrimp feed suppliers increased sharply. Feed samples of eight major brands were collected in the market and analyzed for proximate composition as well as a number of essential nutrients. Some of the nutrient levels detected during the survey, revealed the potential for functional feed additives to optimize nutrient utilization in shrimp feeds in India. COMPARING NOVEL SINGLE CELL PROTEIN TECHNOLOGIES FOR ECONOMICAL FISHMEAL REPLACEMENT David Tze, CEO Novo Nutrients, USA Ongoing growth in aquaculture continues to pressure the decades-long increase in the real cost of fishmeal. With vegetable proteins closing in on maximum beneficial feed inclusion rates, new technologies are emerging to take on the growing opportunity to replace the remaining fishmeal portion, in whole or in part. Given the relative simplicity and efficiency of microbes, compared with reduction fishery species, plants, or insects, it makes clear sense to consider the merits of various microbial single cell protein technologies. These newer technologies include fermentation of bacteria, microalgae, yeast, and other fungi. The substrates may be purchased or received as waste streams. Those inputs might be solid, liquid, or even gas. What challenges will these new technologies face as they break from the lab and race to scale to commercialization? What advantages does each have? Can any economically be produced? If so, what global scale are they likely to achieve? What might be the effect on the feed industry and players within it?

6 INSECTS: THE MISSING LINK IN AQUACULTURE Tarique Arsiwalla, Founder, Protix, Netherlands The aquaculture industry faces severe challenges in sourcing sustainable proteins at a predictable quality and price. Insects are a natural source of nutrients for many fish species and can be produced sustainably. The EU has approved the use of processed insect proteins in aquaculture since July 1, 2017 as it is convinced this new feed ingredient can be produced in a safe manner and offer the quality needed for strong performance and health of the fish. The presentation will focus on the use of insect proteins in diets for shrimp, Atlantic salmon and trout. Trial results will be shown as well as first commercial use of the products in Europe. The opportunities for the Asian market include the use of not only insect proteins, but also insect lipids in diets for young animals and challenged species. The potential for antibiotics reduction will also be touched upon. AQUA FEED PROBIOTICS - CHALLENGES AND OPPORTUNITIES Benedict Standen PhD, Product Manager, BIOMIN GmbH, Austria Beneficial bacteria, probiotics, are becoming increasingly popular in aquaculture. Extensive scientific studies document the advantages of using these additives. Amongst other benefits, probiotics can boost immunity, improve disease resistance, reduce feed conversion ratio, improve growth performance and survival and improve water quality. The benefit obtained is largely dependent on the probiotic formulation. Different probiotic strains have different modes of action, thus they can bring different benefits to the host. For example, Bacillus spp. are capable of producing enzymes contributing to improved digestibility and feed conversion, whereas lactic acid bacteria (LAB) have functions in intestinal colonization and immunity. However, since probiotics are `live' microbial components, they are sensitive to heat and pressure, which makes their inclusion in aqua feeds difficult, regardless of whether pelleting or extrusion technologies are used. Generally speaking, post pelleting application (PPA) is necessary. Utilizing PPA methods, BIOMIN has been developing a novel application for probiotic usage in fish and shrimp feeds, guaranteeing high probiotic viability without compromising the shelf life of the compound feed. This presentation will discuss the benefits of in feed probiotics and summarize results from recent trials, including probiotic application at the feed mill. NUCLEOTIDES AND BIOACTIVE PEPTIDES: BOOSTING THE FUNCTIONALITIES OF AQUAFEEDS Francisco González, Product and Export Manager for APAC in Bioiberica S.A.U., Spain Aquaculture is currently facing several challenges that require novel approaches aimed at providing means to ensure a sustainable and profitable future of the sector. Among other measures, applying innovative solutions such as immunomodulation through nutrition and the use of bioactive peptides as a functional high-quality source of hydrolyzed protein can contribute to achieving health benefits in marine species. The beneficial effects of dietary nucleotides of yeast origin and bioactive peptides from intestinal mucosa, both developed by Bioiberica, have already been

7 demonstrated in several studies in shrimp, salmon, tilapia, meager and sea bream, among other species. Nonetheless, further research is warranted. On-going scientific studies at Bioiberica are currently focused in evaluating the effects of bioactive peptides on growth, nutrient utilization, digestibility, intestinal health and meat quality of rainbow trout (Oncorhynchus mykiss) fed with high levels of vegetable protein, and the effects of dietary nucleotides in white shrimp (Litopenaeus vannamei) affected by Acute Hepatopancreatic Necrosis Disease (AHPND). New product developments are also undergoing and should soon lead to specific formulations for other marine species of high commercial value, such as the sea cucumber. IMMUNITY - THE NEXT FRONTIER IN AQUACULTURE NUTRITION Kabir Chowdhury PhD, Global Technical Manager - Aquaculture, Jefo Nutrition Inc., Canada Sporadic outbreaks of diseases in major commercially important farmed aquatic species have been a major hindrance to the sustainable growth of global aquaculture. Many chemotherapeutics such as antibiotics, drugs and pharmaceutically active compounds are being used either in water or in-feed to control the diseases. However, use of such chemotherapeutics are strongly discouraged because of the fear of resistance to antibiotic or such compunds. In recent years, there has been increasing attention to natural immunostimulants or organic acids or other bio-active compounds for antibiotic free production. Immunostimulants are naturally occurring compounds that modulate the immune system by increasing the host's resistance against diseases caused by pathogens under duress. Several products such as beta-glucans, chitins, mannoproteins, peptidoglycans, alginates, bacterial compounds (lipo-polysaccarides - LPS) and some phytogenic compounds are being used as immunostimulants. This presentation will cover mainly the stress related factors in major farmed species and some relevant immuno-stimulatory solutions available today to the industry. You must register online for Victam Asia 2018 (Trade show registration is free) as well as Aquafeed Horizons Asia conference. Register using the Victam Asia registration portal here: REGISTER If you have any difficulties registration, please contact us here: CONTACT