Potato virus research and the CSU Plant Diagnostic Clinic. Ana Cristina Fulladolsa Research Fellow Bioagricultural Sciences and Pest Management

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1 Potato virus research and the CSU Plant Diagnostic Clinic Ana Cristina Fulladolsa Research Fellow Bioagricultural Sciences and Pest Management

2 Sources: Previous research Potato virus Y University of Wisconsin-Madison Specialty Crops Research Initiative WI Potato and Vegetable Growers Association

3 Sources: Previous research Potato virus Y THE PROBLEM New strains PVY O, PVY NTN, PVY N:O, PVY N-Wi Symptomless carriers ( Typhoid Mary, problem varieties ) Few resistant cultivars

4 Sources: Previous research Potato virus Y THE PROBLEM New strains PVY O, PVY NTN, PVY N:O, PVY N-Wi Symptomless carriers ( Typhoid Mary, problem varieties ) Certified seed RESISTANCE Good diagnostic tools Few resistant cultivars

5 Current research Potato virus Y resistance breeding Potato mop-top virus management Powdery scab management and biology Dr. Amy Charkowski Specialty Crops Research Initiative Plant disease diagnostics CSU Plant Diagnostic Clinic

6 Sources: Potato virus Y resistance breeding Potato virus Y University of Wisconsin Potato Breeding Program RESISTANCE

7 PVY resistance in Solanum chacoense CHC 39-7 Found in Argentina, has genetic resistance to all strains of PVY USDA-SCRI project objective: to develop molecular markers to be used for breeding cultivars with this resistance OUTCOMES: Two linked markers Two diploid germplasm releases One genome sequence

8 Potato mop-top virus and powdery scab management Potato mop-top virus Symptoms continue to develop after storage: Reds > russets Temp fluctuations may accelerate symptom development Sources: Gau et al. 2013, Kalyandurg et al

9 Potato mop-top virus and powdery scab management Powdery scab Cool, wet soil conditions PS lesion Root galls Sources: potato: Sss structures: Gau et al. 2013, PS tuber symptoms and lesion: Melodie Putnam, OSU Plant Clinic; root galls:

10 Potato mop-top virus and powdery scab management Powdery scab Cool, wet soil conditions What we know Spongospora subterranea subsp. subterranea F temp Constant soil moisture > 15% No resistance, some tolerance Resting spores can survive in soil for > 10 years Found worldwide Found in potting mix (peat moss) Sources: potato: Sss structures: Gau et al. 2013, PS tuber symptoms and lesion: Melodie Putnam, OSU Plant Clinic; root galls:

11 2017 powdery scab field trial To evaluate Sss spore accumulation in soil after planting different potato cultivars and using chemical management methods

12 2017 powdery scab field trial To evaluate Sss spore accumulation in soil after planting different potato cultivars and using chemical management methods 3 soil treatments: Omega, RIDEZ, Enhand 6 potato cultivars: Satina, Mozart, Cyclamen (Ciklamen), Soraya, Russet Norkotah 8, and Canela Russet Powdery scab incidence Sss quantification in soil PMTV incidence

13 Current research Potato virus Y resistance breeding Potato mop-top virus management Powdery scab management and biology Plant disease diagnostics CSU Plant Diagnostic Clinic

14 CSU Plant Diagnostic Clinic Potato disease PCR testing services Bacterial ring rot Clavibacter michiganensis detection Cores, tubers Black dot Colletotrichum coccodes detection Tubers, soil, potting mix Blackleg and soft rot Pectobacterium/Dickeya detection Cores, tubers, water Powdery scab Spongospora subterranea detection and quantif Soil, potting mix, water Let us know what you need! NDSU workshop, June 2017

15 CSU Plant Diagnostic Clinic Clinic Contact Ana Cristina Fulladolsa Plant Diagnostic Clinic Lab phone: (970) Clinic mailing address Plant Diagnostic Clinic E215 Plant Sciences Building 1177 Campus Delivery Colorado State University Fort Collins, CO Let us know what you need!