Colorado Potato Certification Service. Annual Summary

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1 Colorado Potato Certification Service Annual Summary 2015 Crop In conjunction with CCPGA Annual Meeting February 8, 2016 Staff Listing page 1 Bulk Shipment History Chart page 2 Acres Entered/Accepted Table page 3 Acres Entered/Accepted Chart page 4 Variety acreage entered 2014/2015 comparison page 5 Grower Entry/Full Certified Acreage Comparison Chart page 6 Grower Entry/Full Certified Acreage Tables page 7-8 Disease Testing Laboratory Summary page 9-10 Tissue Culture Laboratory Summary page Crop Post Harvest Test Summary page PCR Laboratory Summary page PCS General Budget Worksheet Fiscal YTD page 16

2 Colorado Potato Certification Service Colorado State University located at San Luis Valley Research Center 0249 East County Road 9 North Center, CO Fax Manager Andrew Houser, MS Extension 26 andrew.houser@colostate.edu Plant Pathologist Robert D. Davidson, Ph.D. robert.davidson@colostate.edu Disease Lab Supervisor Greg Hess Extension 15 greg.hess@colostate.edu Tissue Culture Specialist Carolyn Keller Extension 29 carolyn.keller@colostate.edu Fieldman Steve Keller Extension 25 steven.keller@colostate.edu Disease Lab Manager Teresa Almeida Extension 18 teresa.almeida@colostate.edu Lab Technician Sarah Shawcroft Extension 18 sarah.shawcroft@colostate.edu Accounting Technician Sharon Yust Extension 17 sharon.yust@colostate.edu Administrative Pro/Coordinator Michelle Leckler Extension 10 michelle.leckler@colostate.edu potatoes.colostate.edu 1

3 Bulk Shipment History Year Out of valley % Out of Valley Local Shipments % Local Totals Acres Accepted ,397 47% 527,603 53% 1,003,000 11, ,075 62% 383,931 38% 1,018,006 12, ,790 56% 522,298 44% 1,191,088 10, ,081 64% 451,906 36% 1,260,987 13, ,194,801 76% 370,519 24% 1,565,320 14, ,013,069 72% 386,727 28% 1,399,796 15, ,683 73% 342,880 27% 1,250,563 13, ,109,101 76% 349,678 24% 1,458,779 15, ,078,482 75% 359,283 25% 1,437,765 13, ,760 60% 441,006 40% 1,093,766 12, ,933 62% 407,103 38% 1,078,036 12, ,781 60% 443,984 40% 1,114,765 12, ,893 55% 523,111 45% 1,166,004 10, ,336 60% 421,660 40% 1,056,996 12, ,789 57% 404,465 43% 932,254 11, ,594 60% 471,898 40% 1,194,492 12, ,880 57% 445,445 43% 1,027,325 13, ,588 54% 401,741 46% 866,329 13, ,425 48% 452,131 52% 866,556 9, ,421 56% 361,573 44% 823,994 10,975 1,400,000 Shipping History c w t S h i p p e d 1,200,000 1,000, , , , , Year Out of valley Local Shipments 2

4 Colorado Potato Certification Program Cumulative Results Acres Acres Year Entered Approved % BRR % PLRV % MO % BL % Admin. % ,595 5, ,529 5, ,573 5, ,620 5, ,898 6, ,184 5, ,931 6, ,597 9, , ,867 8, , ,552 7, ,113 6, ,256 7, ,572 8, , ,671 7, , ,233 9, , ,142 11, , ,977 9, , ,385 10, , ,224 10, ,438 10, ,210 9, ,271 9, ,702 10, ,508 9, ,878 10, ,928 11, ,225 12, ,571 10, ,678 13, ,883 14, ,831 15, ,906 13, , , , ,400 15, , ,705 13, ,933 12, , ,289 12, , ,268 12, ,429 10, , ,373 12, , ,326 11, , ,122 12, ,998 13, ,918 13, , ,256 9, , ,730 10, , ,667 9, ,

5 Acres Entered/Accepted 18,000 16,000 Acres Entered Acres Accepted 14,000 12,000 10,000 8,000 6,000 4,000 2,

6 Acres Entered Comparison, Top 50 Cultivars Cultivar 2014 Acreage 2015 Acreage Change Russet Norkotah % Canela Russet % Classic Russet % Rio Grande Russet % Teton Russet % Centennial Russet % Fortress Russet % Chipeta % Alegria % LaRatte % Satina % Yukon Gold % Innovator % Atlantic % Soraya % Mozart % Desiree % Latona % Chieftain % NY % Molli % Rose Finn Apple % Mesa Russet % Lamoka % Russet Nugget % Asterix % DT6063-1R % Anuschka % CalWhite % Midnight Moon % Red Thumb % Austrian Crescent % Jelly % Colorado Rose % Elfe % Cherie % Yukon Gem % Purple Majesty % Masquerade % Red LaSoda Sel % Ludmilla % Allora % Mercury Russet % German Butterball % Nicola % French Fingerling % Crestone Russet % Kennebec % NY % 5

7 Acres Entered: Grower Entry as compared to Full Certified T o t a l A c r e a g e Grower Entry Full Certified Year 6

8 2015 FULL CERTIFIED GROWERS CERTIFICATION PROGRAM RESULTS ACREAGE REJECTED OR WITHDRAWN TOTAL ACREAGE REASON CULTIVAR Entered Rejected WithdrawnTotal LR MO BL VM ADMIN AC W Alegria CalWhite Canela Russet Centennial Russet Chipeta CO RU CO W/Y CO R CO RU CO RU CO R Colorado Rose Desiree Elfe Fortress Russet Kennebec Mercury Russet Mesa Russet Molli Purple Majesty Red LaSoda Red McClure Red Sonia Red Thumb Rio Grande Russet Rose Finn Apple Russet Norkotah Russet Norkotah Russet Norkotah Russet Norkotah Russet Norkotah Soraya Yukon Gold Misc TOTAL LR=Leafroll, MO=Mosaic, BL=Blackleg, VM=Variety mix, Admin=Administrative reasons 2015 FULL CERTIFIED GROWERS CERTIFICATION PROGRAM RESULTS DOWNGRADED ACRES TOTAL ACREAGE CULTIVAR Entered Total LR MO BL VM ADMIN Russet Norkotah Russet Norkotah Rio Grande Russet Mesa Russet Red LaSoda Sel Latona Canela Russet Russet Nugget Chipeta Purple Majesty Atlantic Centennial Russet Crestone Russet CO R/R Banana AC R/Y ATC W/Y CO W Miscellaneous Total

9 Grower Entry Colorado Potato Certification Program Cumulative Acres TOTAL ACREAGE REASON Cultivar Entered Rejected Withdrawn Total LR MO BL VM ADMIN Blossom Butte Canela Russet Caribe Chieftain Ciklamen Classic Russet Fortress Russet Garnett Chili Keuka Gold Ozette Purple Peruvian Purple Viking Red Companion Red Norland Russet Norkotah Russet Nugget Satina Yukon Gold Misc TOTAL LR=Leafroll, MO=Mosaic, BL=Blackleg, VM=Variety mix, Admin=Administrative reasons Grower Entry - Colorado Potato Certification Program Cumulative Results Acres Acres Year Entered Approve % BRR % PLRV % MO % BL % Admin. % ,496 2, ,647 3, ,828 2, Full Certified Grower - Colorado Potato Certification Program Cumulative Results Acres Acres Year Entered Approve % BRR % PLRV % MO % BL % Admin. % ,760 7, ,082 7, ,838 7,

10 2015 PCS Disease Testing 11 CCPGA Greenhouse Growers 67 greenhouse crops grown in 2015 (2 inspections per crop) 1.7 million plants produced ELISA Testing for 2016 *Decrease in summer testing *Increase in greenhouse testing *Increase in clonebank and PHT sprout testing Projections for anticipating additional commercial lots being tested at post harvest in conjunction with the Colorado Seed Act Bacterial Testing for PHT, Greenhouse tubers and clonebanks for PHT Clavibacter michiganensis (BRR) tests (for possible export) Pectobacterium (Erwinia) tests for Greenhouse tubers 307 Clavibacter michiganensis (BRR) tests for Greenhouse tubers 695 Bacterial tests for PCS Clonebank 900 Potato Spindle Tuber Viroid Test Disease Confirmation Testing - Calendar Year 2015 Special Request Testing from Individual Growers Suspect pathogens tested: (Scientific Name) Candidatus Liberibacter Sol. Meloidogyne spp. Tobravirus Furovirus Clavibacter michiganensis Tospovirus Fusarium Pectobacterium Collectotrichum Helminthosporium solani Phytophthora infestans Phythium Alternaria Phytophthora erythroseptica Spongospora Subterranea (Common Name) Zebra Chip Root Knot Nematode Tobacco Rattle Virus Potato Mop Top Virus Bacterial Ring Rot Tomato Spotted Wilt Virus Dry Rot Erwinia/Soft Rot Black Dot Silver Scurf Late Blight Leak Early Blight Pink Rot Scab, Common / Powdery Scab 21 requests from CCPGA Seed Growers 29 requests from Commercial Growers 9

11 Year Acres Entered Summer field leaves picked Summer Elisa wells Insp. Samp. PCS CB Elisa wells ELISA TESTING DATA Growers GH/CB Elisa wells. Breeding SLVRC GH # of wells(pht leaves and Sprouts) Addiional N ational Surveys PVYn PCR Research Commercial Testing PHT PVY-n Leaves Wells , , ,182 10,058 21,004 5, , , ,150 90,873 13,672 20,697 17, , , ,882 86,763 9,994 23,201 26, , , , ,511 7,304 17,631 11,517 2, , , ,265 98,782 13,485 19,148 57, , , , ,194 12,848 17,054 12,516 16, , , ,980 96,692 10,842 20,944 34, , , ,688 97,402 9,141 17,209 18,122 7, , , ,340 92,231 24,560 15,125 47,228 9, , , ,070 74,860 23,280 17,821 49, , , ,400 88,178 10,595 23,830 17, , , ,643 97,282 6,429 30,608 12, , , ,385 87,909 2,168 7,324 25,509 13, , , ,554 84,936 1,995 9,204 26,208 24, , , ,000 69,646 1,590 6,781 22,081 36,789 15, , , , ,552 1,911 6,571 20,188 2,064 15,738 10, , ,667 31,030 12,594 2,174 8,360 21, ,032 1,996 2, ,088 69,481 Totals wells 10

12 Tissue Culture Lab Potato Certification Service 2015 Summary Active Clone Bank: Cultivars actively propagated and maintained 200 Cultivars: 4 test tubes each = 800 tubes Each tube is cut 3 times/year Also cut twice/year for testing/grow out/tuber identification. Long Term Storage Clone Bank: Maintenance of cultivars rarely used 320 Cultivars: 4 test tubes each = 1,280 Tubes 1/3 of the clone bank is cut each year Miscellaneous Cultivars Not In Clone Bank: Primarily experimental cultivars for breeding purposes 48 Varieties: 4 test tubes each = 192 Tubes Each tube is cut 3 times/year Approximate Number of Plantlets Sent Out Year Colorado SLVRC Out of Misc. Growers State Labs ,760 44, , ,582 44, ,602 Initiation & Clean Up Starting from tubers, initiated 8 new cultivars into tissue culture. Used heat and chemical treatment to clean viruses from 5 of these. New Cultivars Received 48 additional varieties in tissue culture for growers. Breeding Program/Special Projects Initiated new cultivars into tissue culture, maintained varieties kept for breeding/research purposes, and cut plantlets for the breeding program greenhouse. Continued to spend some time on the cryotherapy project. A cooler malfunction froze some varieties which led to many extra hours of work. Record Keeping Extensive paperwork to document all testing done on active and long term storage clone banks, orders cut for growers and other labs, and to track the virus clean up process. 11

13 2015 PHT Results Summary (PHT Readings taken December 8 23, 2015 in Oahu, Hawaii.) All Seed Lots Mosaic level # lots % lots # acres % acres 0% % % >0% 3% % % % % % % % % 5.1% 8.0% % % >8.0% % % Total Full Certified Lots Grower Entry Lots Mosaic level # lots % lots # acres % acres Mosaic level # lots % lots # acres % acres 0% % % 0% % % >0% 3% % % >0% 3% % % % % % % % % % % % % 4 4.7% % 5.1% 8.0% % % 5.1% 8.0% 3 3.5% % >8.0% % % >8.0% 5 5.9% % Total Total Total number of lots & acres with PVY N/NTN in 2015 PHT (only lots with 1 5% PVY were tested). These leaves were tested using ELISA in Hawaii. This testing was done on seed lots originating in Colorado in order to be in compliance with the Colorado Seed Law. Lots with PVY N/NTN % lots tested out of all lots with # lots % lots PVY # acres % acres % 48.4% % Acres with PVY N/NTN % acres tested out of all lots with PVY 43.2% Leaves with PVY N/NTN # plants # plants with % PVY N/NTN in overall tested PVY N/NTN PVY population % 12

14 2015 PHT Results Percent Acreage Under 5% for the Top Six Russet Cultivars % 3.1 5% 70.0 % Acreage Norkotahs Canela Russet Teton Russet Rio Grande Russet % Mosaic Classic Russet Centennial Russet 1,363 Acres 838 Acres 574 Acres 383 Acres 355 Acres 340 Acres 13

15 2015 PCR Disease Testing Summary Prior to 2015, PCS sent samples out-of-state for PCR (Polymerase Chain Reaction) required viral & bacterial diagnostics. In 2014 and 2015 Greg has been focusing on implementing laboratory molecular procedures for the detection of potato pathogens. PCR testing protocols and procedures have been developed, and are being developed, for pathogens affecting the SLV potato industry. The following is a summary of the PCR testing conducted during This summary includes: testing requested by growers, specialty crop grant PVY survey testing, and testing performed for the validation of PCR protocols. Overall Testing Performed Target Pathogen Toatal Reactions BACTERIA BRR/CMS Clavibacter michiganensis 1, ,629 Soft Rot/Black Leg Pectobacter/Dickeya spp VIRUSES PVY Potato Virus Y (N,O,N:O,Wi,NTN) 13,595* 4,000+ 5,900+ Corkey (TRV) Spraing (PMTV) Tobacco Rattle Virus Potato Mop Top Virus Grower Leaves Tubers Other Virus PVA, M, S, X, etc * both reverse transcription (RT) and forward qpcr reactions BACTERIA Testing: validate, training, research, grower samples,& contols [99% CMS] Total Bac. Reactions Grower Lots Gower Reactions Grower Tubers Jan Feb Mar Apr M/Jn/Jl Aug Sep Oct Nov Dec TOTALS: ,999 3,200 1, ,629 VIRUS TESTING: PVY Survey Summary by county COUNTY GROWERS VARIETIES LOTS COMMERCIAL SEED ACRES PLANTS TUBERS ALAMOSA COSTILLA RIO GRANDE SAGUACHE TOTAL UNIQUE PVY Survey by variety Strain percent of PVY infections detected VARIETY LOTS ACRES SAMPLES N:Wi O NTN N:O N MULTI 90M ATLANTIC CANELA R CENTENNIAL R CHIEFTAIN CHIPETA CLASSIC CO. ROSE MESA R R. NORK R. NORK R. NORK R. NORK R. NORK R. NUGGET PACIFIC R RIO GRANDE R TETON R OTHER VAR Total % 35.90% 0.02% 0.00% UND 0.12% 14

16 PRICE LIST for PCR Testing 1 Sample/Well Each Additional Well 400 tuber sample TEST PATHOGEN Leaf/Sprout/Core Leaves 1 Cores 1,2 Leaves 3 Cores 2 Blackleg BL/SR (Pectobacterium, Dickeya ) NA NA NA NA NA CMS Bacterial Ring Rot (C. michigenensis ) $40 NA $40 $320 AMV Alfalfa Mosaic Virus NA NA NA NA NA LB Late Blight (P. infestans ) NA NA NA NA NA PLRV Potato Leaf Roll Virus NA NA NA NA NA PMTV Potato Mop Top Virus $35 $13 $15 $650 $750 TRV Tobacco Rattle Virus $35 $13 $15 $650 $750 PMTV & TRV Potato Mop Top Virus & Tobacco Rattle Virus $50 $20 $25 $750 $850 PVA Potato Virus A NA NA NA NA NA PVM Potato Virus M NA NA NA NA NA PVS Potato Virus S NA NA NA NA NA PVX Potato Virus X NA NA NA NA NA PVY PVY Strain ID $35 $13 $15 $650 $750 PVY Potato Virus Y $35 $13 $15 $650 $750 PVY N/NTN Potato Virus Y (N/NTN Strain) $35 $13 $15 $650 $750 TSWV Tomato Spotted Wilt Virus NA NA NA NA NA 15

17 2015 Crop January 31, /2016 Fiscal Year To Date Expenses Colorado Potato Certification Service (PCS) General Budget Worksheet SP # PCS Approved Budget (7/1/15-6/30/16) $757, Fiscal YTD Payments to CSU by CCPGA $197, PCS Current Income for services rendered: Acreage Fees $321, Disease Testing $43, PCR Testing $ Post Harvest Tests $32, Bulk Certificates $69, Tissue Culture $1, Misc $ Total: $469, PCS Expenses to date: $471, PCS current receipts over current expense: -$1, PCS Current Income less CCPGA Payments: $271, PCS Current Income vs. approved budget: -$287,