Haemostasis Antibodies

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Marjory Hensley
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1 HAEMOSTASIS ANTIBODIES FROM CEDARLANE

2 NEW! The study of haemostasis and thrombosis has become one of the most rapidly growing fields in medical research. An unprecedented amount of research is currently focused on the study of the regulatory mechanisms involved in blood clot formation, subsequent lysis, and potential pharmacological intervention of these processes. Cedarlane offers a complete line of antibodies to haemostasis related antigens. The high quality and purity of these antibodies make them valuable tools for applications including ELISA, immunohistochemistry, immunoblotting (Western blot) and immunoprecipitation techniques (including immunodiffusion and rocket immunoelectrophoresis). Cedarlane offers an expanding range of new monoclonal antibodies to various haemostasis related antigens, providing customers a more comprehensive array of tools for the study of haemostasis and thrombosis. These antibodies have been tested for suitability for use in Western Blotting and ELISA. Featured Antibodies Factor VII/VIIA Purified AA-3 mouse IgG1 Human E, WB 200 μg CL2763AP 259 Factor VII/VIIA Biotin AA-3 mouse IgG1 Human E, WB 100 μg CL2763B 199 Factor VII/VIIa Purified AD-1 mouse IgG1 Human E, WB 200 μg CL2764AP 259 Factor VII/VIIa Biotin AD-1 mouse IgG1 Human E, WB 100 μg CL2764B 199 Factor VII/VIIa (Calcium Dependent) Purified CaFVII-22 mouse IgG1 Human E, WB 200 μg CL2762AP 259 Factor VII/VIIa (Calcium Dependent) Biotin CaFVII-22 mouse IgG1 Human E, WB 100 μg CL2762B 199 Factor IX/IXa (Calcium Dependent) Purified CaFIX-1 mouse IgG1 Human E, WB 200 μg CL2765AP 259 Factor IX/IXa (Calcium Dependent) Biotin CaFIX-1 mouse IgG1 Human E, WB 100 μg CL2765B 199 Factor X/Xa (Calcium Dependent) Purified CaFX-50 mouse IgG1 Human E, WB 200 μg CL2768AP 259 Factor X/Xa (Calcium Dependent) Biotin CaFX-50 mouse IgG1 Human E, WB 100 μg CL2768B 199 Platelet Factor 4 Purified RTO mouse IgG2b Human E, WB 200 μg CL2766AP 259 Platelet Factor 4 Biotin RTO mouse IgG2b Human E, WB 100 μg CL2766B 199 Platelet Factor 4 (requires heparin for binding) Purified KKO mouse IgG2b Human E, WB 200 μg CL2767AP 259 Platelet Factor 4 (requires heparin for binding) Biotin KKO mouse IgG2b Human E, WB 100 μg CL2767B 199 Protein C/APC (Calcium Dependent) Purified CaC-11 mouse IgG1 Human E, WB 200 μg CL2769AP 259 Proteinase Inhibitor 8 Purified anti-pi8k mouse IgG1 Human E, WB 200 μg CL2770AP 259 Prothrombin (Calcium Dependent) Purified CaPro-20 mouse IgG1 Human E, WB 200 μg CL2761AP 259 Prothrombin (Calcium Dependent) Biotin CaPro-20 mouse IgG1 Human E, WB 100 μg CL2761B 199 Legend for Applications: AC=Affinity Chromatography AGG=Agglutination C=Cryostat Sections CT=Cytotoxicity Assay D=Depletion E=ELISA EC=ELISA (Capture) ED=ELISA (Detection) F=Flow Cytometry FN=Functional Assay IC=Immunocytochemistry ID=Immunodiffusion IF=Immunofluorescence IP=Immunoprecipitation IV=In vivo Assay N=Neutralization P=Paraffin-Embedded Sections R=RIA WB=Western Blotting NEW! Cedarlane s Buffer Pack Cat # CL20300 Price (USD): $ Cedarlane s Buffer Pack for Haemostasis Paired Antibodies consists of assay plates, general purpose buffers and materials for the preparation and performance of ELISA assays. This Buffer Pack provides sufficient reagents for 5 x 96 well assays. The components of the Buffer Pack can be used in the development of in-house immunoassays and are especially suited for use with a number of Cedarlane s paired antibody sets, including the following: CL20067K CL20067K-RB CL20111K CL20225K CL20030K CL20403K CL20245K CL20250K CL20055K CL20057K CL20096K CL20106K CL20295K CL20201K CL20220K CL20086K CL20003K CL20002K CL20027K CL20090K CL20200K CL20116K CL20017K CL20048K CL20049K CL20087K 2 HAEMOSTASIS ANTIBODIES FROM CEDARLANE

3 Working concentrations for ELISA and Immunoblotting applications General recommendations only and may not apply to all products listed. For more information, please contact or your local distributor. Format Immunoblotting (ECL detection) ELISA (capture) ELISA (detecting) Purified (Whole IgG) 5 25 μg/ml μg/ml NA Affinity Purified μg/ml 10 μg/ml NA HRPO (Peroxidase-labelled IgG) 5 μg/ml NA 10 μg/ml HRPO (Aff. Pur. Peroxidase-labelled IgG) 1 μg/ml NA 2 μg/ml α1-antitrypsin Purified sheep IgG Human IP 10 mg CL20000A 96 α1-antitrypsin Affinity Purified sheep IgG Human E 0.5 mg CL20000AP 184 α1-antitrypsin HRPO (Affinity Pur) sheep IgG Human E 0.1 mg CL20000APHP 162 α1-antitrypsin Purified goat IgG Human IP 10 mg CL20001A 108 α1-antitrypsin Affinity Purified goat IgG Human E 0.5 mg CL20001AP 184 α2-antiplasmin Purified goat IgG Human IP, D 5 mg CL20005A 136 α2-antiplasmin Affinity Purified goat IgG Human E 0.5 mg CL20005AP 262 α2-antiplasmin HRPO goat IgG Human E 0.2 mg CL20005HP 209 α2-antiplasmin Purified sheep IgG Human E 10 mg CL20006A 146 α2-macroglobulin Purified goat IgG Human IP 10 mg CL20010A 96 α2-macroglobulin Affinity Purified goat IgG Human E, WB 0.5 mg CL20010AP 184 α2-macroglobulin HRPO (Affinity Pur) goat IgG Human E, WB 0.1 mg CL20010APHP 162 Antithrombin III Inhibitor and Control Antibodies Human N 10 det CL20007N 116 Antithrombin III Purified sheep IgG Human N, D, IP 10 mg CL20015A 80 Antithrombin III Affinity Purified sheep IgG Human E, WB, IP 0.5 mg CL20015AP 209 Antithrombin III HRPO (Affinity Pur) sheep IgG Human E, WB 0.1 mg CL20015APHP 186 Antithrombin III Biotin sheep IgG Human E, WB 0.1 mg CL20015B 186 Antithrombin III FITC sheep IgG Human IF 0.1 mg CL20015F 186 Antithrombin III Purified goat IgG Human IP, N, D 10 mg CL20016A 96 Antithrombin III Affinity Purified goat IgG Human E, IP 0.5 mg CL20016AP 236 Antithrombin III Purified sheep IgG Rabbit E, IP 10 mg CL20018A-RB 117 Antithrombin III Affinity Purified sheep IgG Rabbit E, IP 0.5 mg CL20018AP-RB 249 Antithrombin III HRPO sheep IgG Rabbit E 0.2 mg CL20018HP-RB 209 Complement Component C1 Esterase Inhibitor (C1 Inactivator) Complement Component C1 Esterase Inhibitor (C1 Inactivator) Complement Component C1 Esterase Inhibitor (C1 Inactivator) Purified goat IgG Human IP 5 mg CL20023A 162 Affinity Purified goat IgG Human E, IP 0.5 mg CL20023AP 292 HRPO goat IgG Human E 0.2 mg CL20023HP 209 Factor H Purified OX-24 mouse IgG1 Human E 250 μg CL7672AP 200 Factor H Biotin OX-24 mouse IgG1 Human E 100 μg CL7672B 115 Factor H FITC OX-24 mouse IgG1 Human E 100 μg CL7672F 126 Legend for Applications: AC=Affinity Chromatography AGG=Agglutination C=Cryostat Sections CT=Cytotoxicity Assay D=Depletion E=ELISA EC=ELISA (Capture) ED=ELISA (Detection) F=Flow Cytometry FN=Functional Assay IC=Immunocytochemistry ID=Immunodiffusion IF=Immunofluorescence IP=Immunoprecipitation IV=In vivo Assay N=Neutralization P=Paraffin-Embedded Sections R=RIA WB=Western Blotting HAEMOSTASIS ANTIBODIES FROM CEDARLANE 3

4 (continued) Factor IX Purified sheep IgG Human E 10 mg CL20039A 136 Factor IX Affinity Purified sheep IgG Human EC 0.5 mg CL20039AP 249 Factor IX HRPO (Affinity Pur) sheep IgG Human E 0.1 mg CL20039APHP 182 Factor IX Purified goat IgG Human IP, D, WB 10 mg CL20040A 122 Factor IX Affinity Purified goat IgG Human E 0.5 mg CL20040AP 249 Factor IX HRPO (Affinity Pur) goat IgG Human E, WB 0.1 mg CL20040APHP 182 Factor IX Biotin goat IgG Human E, WB 0.1 mg CL20040B 182 Factor IX FITC goat IgG Human IF 0.1 mg CL20040F 182 Factor IX HRPO goat IgG Human E, WB 0.2 mg CL20040HP 182 Factor IX/IXa (Calcium Dependent) Purified CaFIX-1 mouse IgG1 Human E, WB 200 μg CL2765AP 259 Factor IX/IXa (Calcium Dependent) Biotin CaFIX-1 mouse IgG1 Human E, WB 100 μg CL2765B 199 Factor V Purified sheep IgG Bovine IP, D 10 mg CL20025A-B 136 Factor V Affinity Purified sheep IgG Bovine WB 0.5 mg CL20025AP-B 249 Factor V HRPO sheep IgG Bovine E 0.2 mg CL20025HP-B 209 Factor V Purified sheep IgG Human IP, N, D, WB 10 mg CL20025A 136 Factor V Affinity Purified sheep IgG Human EC 0.5 mg CL20025AP 317 Factor V Biotin sheep IgG Human E 0.1 mg CL20025B 209 Factor V FITC sheep IgG Human IF 0.1 mg CL20025F 209 Factor V HRPO sheep IgG Human E 0.2 mg CL20025HP 209 Factor VII Purified sheep IgG Human IP, N, D 10 mg CL20030A 136 Factor VII Affinity Purified sheep IgG Human E 0.5 mg CL20030AP 257 Factor VII Biotin sheep IgG Human E 0.1 mg CL20030B 209 Factor VII FITC sheep IgG Human IF 0.1 mg CL20030F 209 Factor VII HRPO sheep IgG Human E 0.2 mg CL20030HP 209 Factor VII/VIIA Purified AA-3 mouse IgG1 Human E, WB 200 μg CL2763AP 259 Factor VII/VIIA Biotin AA-3 mouse IgG1 Human E, WB 100 μg CL2763B 199 Factor VII/VIIa Purified AD-1 mouse IgG1 Human E, WB 200 μg CL2764AP 259 Factor VII/VIIa Biotin AD-1 mouse IgG1 Human E, WB 100 μg CL2764B 199 Factor VII/VIIa (Calcium Dependent) Purified CaFVII-22 mouse IgG1 Human E, WB 200 μg CL2762AP 259 Factor VII/VIIa (Calcium Dependent) Biotin CaFVII-22 mouse IgG1 Human E, WB 100 μg CL2762B 199 Factor VIII.vWF (Non-reactive with rat) Ascites 2F2-A9 mouse IgG1 Human IF, C, P, R 0.5 ml CL1303A 299 Factor VIII:C (Non-reactive with mouse) Purified sheep IgG Human E, IP, N, ID 10 mg CL20035A 136 Factor VIII:C (Non-reactive with mouse) Affinity Purified sheep IgG Human E, N, WB 0.5 mg CL20035AP 317 Factor VIII:C (Non-reactive with mouse) Biotin sheep IgG Human E 0.1 mg CL20035B 209 Factor VIII:C (Non-reactive with mouse) FITC sheep IgG Human IF 0.1 mg CL20035F 209 Factor VIII:C (Non-reactive with mouse) HRPO sheep IgG Human E 0.2 mg CL20035HP 209 Factor X Purified goat IgG Human IP, ID 10 mg CL20045A 122 Factor X Affinity Purified goat IgG Human EC 0.5 mg CL20045AP 249 Factor X HRPO goat IgG Human ED 0.2 mg CL20045HP 182 Factor X HRPO rabbit IgG Human WB 0.2 mg CL20046HP 209 Factor X Affinity Purified sheep IgG Human EC 0.5 mg CL20047AP 249 Legend for Applications: AC=Affinity Chromatography AGG=Agglutination C=Cryostat Sections CT=Cytotoxicity Assay D=Depletion E=ELISA EC=ELISA (Capture) ED=ELISA (Detection) F=Flow Cytometry FN=Functional Assay IC=Immunocytochemistry ID=Immunodiffusion IF=Immunofluorescence IP=Immunoprecipitation IV=In vivo Assay N=Neutralization P=Paraffin-Embedded Sections R=RIA WB=Western Blotting 4 HAEMOSTASIS ANTIBODIES FROM CEDARLANE

5 (continued) Factor X Biotin sheep IgG Human EC 0.1 mg CL20047B 182 Factor X FITC sheep IgG Human IF 0.1 mg CL20047F 182 Factor X/Xa (Calcium Dependent) Purified CaFX-50 mouse IgG1 Human E, WB 200 μg CL2768AP 259 Factor X/Xa (Calcium Dependent) Biotin CaFX-50 mouse IgG1 Human E, WB 100 μg CL2768B 199 Factor XI Purified goat IgG Human IP, N, D, WB 5 mg CL20050A 136 Factor XI Affinity Purified goat IgG Human E 0.5 mg CL20050AP 257 Factor XI Biotin goat IgG Human E 0.1 mg CL20050B 209 Factor XI FITC goat IgG Human IF 0.1 mg CL20050F 209 Factor XI HRPO goat IgG Human E, WB 0.2 mg CL20050HP 182 Factor XI Purified sheep IgG Human IP 5 mg CL20051A 136 Factor XI Affinity Purified sheep IgG Human E 0.5 mg CL20051AP 257 Factor XII Purified sheep IgG Human IP, D 5 mg CL20054A 136 Factor XII Affinity Purified sheep IgG Human E 0.5 mg CL20054AP 262 Factor XII Purified goat IgG Human IP, N, D 5 mg CL20055A 136 Factor XII Affinity Purified goat IgG Human E 0.5 mg CL20055AP 262 Factor XII HRPO goat IgG Human E, WB 0.2 mg CL20055HP 182 Factor XIII Purified sheep IgG Human IP, D 10 mg CL20057A 136 Factor XIII HRPO sheep IgG Human E 0.2 mg CL20057HP 209 Factor XIII (A subunit) Purified sheep IgG Human IP, D 10 mg CL20056A 136 Factor XIII (A subunit) Affinity Purified sheep IgG Human E 0.5 mg CL20056AP 268 Factor XIII (A subunit) Biotin sheep IgG Human E 0.1 mg CL20056B 209 Factor XIII (A subunit) FITC sheep IgG Human IF 0.1 mg CL20056F 209 Factor XIII (A subunit) HRPO sheep IgG Human E 0.2 mg CL20056HP 209 Fibrin fragment E Purified sheep IgG Human IP 10 mg CL20065A 122 Fibrin fragment E HRPO sheep IgG Human E 0.2 mg CL20065HP 182 Fibrinogen Ascites 2C2-G7 mouse IgG1 Human FN, E, WB, R 0.5 ml CL1302A 299 Fibrinogen Purified sheep IgG Human P, WB, ID, IP 10 mg CL20067A 122 Fibrinogen Affinity Purified sheep IgG Human EC, WB 0.5 mg CL20067AP 162 Fibrinogen HRPO (Affinity Purified) sheep IgG Human E 0.1 mg CL20067APHP 182 Fibrinogen HRPO sheep IgG Human E, P 0.2 mg CL20067HP 182 Fibrinogen Purified sheep IgG Rabbit IP, P 10 mg CL20067A-RB 122 Fibrinogen Affinity Purified sheep IgG Rabbit E, P 0.5 mg CL20067AP-RB 209 Fibrinogen HRPO sheep IgG Rabbit E, P 0.2 mg CL20067HP-RB 182 Fibrinogen gamma prime (gamma specific) (Peak 2 Fibrinogen) Fibrinogen gamma prime (gamma specific) (Peak 2 Fibrinogen) Purified sheep IgG Human E 5 mg CL20075A 122 HRPO sheep IgG Human E 0.2 mg CL20075HP 182 Fibrinopeptide A (FPA:A 1-16 specific) Purified sheep IgG Human E 10 mg CL20068A 203 Fibrinopeptide A (FPA:A 1-16 specific) Affinity Purified sheep IgG Human E 0.5 mg CL20068AP 317 Fibrinopeptide A (FPA:A 1-16 specific) HRPO sheep IgG Human E 0.2 mg CL20068HP 317 Fibronectin Ascites 2B6-F9 mouse IgG1 Human WB, E, R 0.5 ml CL1301A 299 Fibronectin Purified rabbit IgG Mouse IFA, IHC, E, (SP) RIA, IB/WB 0.5 ml CL54951AP 367 Legend for Applications: AC=Affinity Chromatography AGG=Agglutination C=Cryostat Sections CT=Cytotoxicity Assay D=Depletion E=ELISA EC=ELISA (Capture) ED=ELISA (Detection) F=Flow Cytometry FN=Functional Assay IC=Immunocytochemistry ID=Immunodiffusion IF=Immunofluorescence IP=Immunoprecipitation IV=In vivo Assay N=Neutralization P=Paraffin-Embedded Sections R=RIA WB=Western Blotting HAEMOSTASIS ANTIBODIES FROM CEDARLANE 5

6 (continued) Fibronectin Purified rabbit IgG Human E 0.5 ml CL54911AP 182 Glycoprotein IIb/IIIa Purified sheep IgG Human WB 10 mg CL20069A 162 Heparin Cofactor-II Purified goat IgG Human IP, N 5 mg CL20070A 136 Heparin Cofactor-II Affinity Purified goat IgG Human E, WB 0.5 mg CL20070AP 262 Heparin Cofactor-II HRPO (Affinity Pur) goat IgG Human E, WB 0.1 mg CL20070APHP 186 Heparin Cofactor-II Inhibitor and Control Antibodies Human N 10 det CL20072N 116 Hirudin (Leech) Purified sheep IgG Human IP, D, N 10 mg CL20073A 136 Hirudin (Leech) Affinity Purified sheep IgG Human E 0.5 mg CL20073AP 262 Hirudin (Leech) HRPO sheep IgG Human E 0.2 mg CL20073HP 194 Kininogen Purified sheep IgG Human IP 10 mg CL20027A 136 Kininogen Affinity Purified sheep IgG Human IP 0.5 mg CL20027AP 243 Kininogen HRPO (Affinity Pur) sheep IgG Human E 0.1 mg CL20027APHP 182 KLK14 Serum (Affinity Pur) rabbit IgG Human E 100 μg CLAS Neutrophil Elastase Purified sheep IgG Human IP 10 mg CL20080A 122 Neutrophil Elastase Affinity Purified sheep IgG Human E 0.5 mg CL20080AP 229 Neutrophil Elastase HRPO sheep IgG Human E 0.2 mg CL20080HP 194 Plasminogen Purified goat IgG Human IP 5 mg CL20085A 101 Plasminogen Affinity Purified goat IgG Human E, IP 0.5 mg CL20085AP 182 Plasminogen HRPO (Affinity Pur) goat IgG Human E, WB 0.1 mg CL20085APHP 162 Plasminogen Purified sheep IgG Human IP 5 mg CL20087A 101 Plasminogen Affinity Purified sheep IgG Human E 0.5 mg CL20087AP 182 Plasminogen Activator Inhibitor Type 1 Purified sheep IgG Human E, IP 10 mg CL20140A 141 Plasminogen Activator Inhibitor Type 1 HRPO sheep IgG Human E 0.2 mg CL20140HP 230 Platelet Factor 4 Purified sheep IgG Human IP 10 mg CL20145A 136 Platelet Factor 4 Affinity Purified sheep IgG Human E 0.5 mg CL20145AP 262 Platelet Factor 4 HRPO sheep IgG Human E 0.2 mg CL20145HP 209 Platelet Factor 4 Purified RTO mouse IgG2b Human E, WB 200 μg CL2766AP 259 Platelet Factor 4 Biotin RTO mouse IgG2b Human E, WB 100 μg CL2766B 199 Platelet Factor 4 (requires heparin for binding) Purified KKO mouse IgG2b Human E, WB 200 ug CL2767AP 259 Platelet Factor 4 (requires heparin for binding) Biotin KKO mouse IgG2b Human E, WB 100 ug CL2767B 199 Prekallikrein Purified sheep IgG Human IP, D, N 10 mg CL20090A 136 Prekallikrein Affinity Purified sheep IgG Human IP, D, N 0.5 mg CL20090AP 243 Prekallikrein HRPO (Affinity Pur) sheep IgG Human E 0.1 mg CL20090APHP 182 Protein C Purified goat IgG Human IP, D 5 mg CL20095A 136 Protein C Affinity Purified goat IgG Human E 0.5 mg CL20095AP 265 Protein C Purified sheep IgG Human IP, D 10 mg CL20096A 136 Protein C Affinity Purified sheep IgG Human E, WB, C 0.5 mg CL20096AP 257 Protein C HRPO sheep IgG Human E 0.2 mg CL20096HP 182 Protein C Purified mouse IgG Human E, WB 0.5 mg CL20098A 254 Protein C Inhibitor Purified goat IgG Human IP, D 10 mg CL20100A 136 Legend for Applications: AC=Affinity Chromatography AGG=Agglutination C=Cryostat Sections CT=Cytotoxicity Assay D=Depletion E=ELISA EC=ELISA (Capture) ED=ELISA (Detection) F=Flow Cytometry FN=Functional Assay IC=Immunocytochemistry ID=Immunodiffusion IF=Immunofluorescence IP=Immunoprecipitation IV=In vivo Assay N=Neutralization P=Paraffin-Embedded Sections R=RIA WB=Western Blotting 6 HAEMOSTASIS ANTIBODIES FROM CEDARLANE

7 (continued) Protein C Inhibitor Affinity Purified goat IgG Human E 0.5 mg CL20100AP 262 Protein C Inhibitor HRPO goat IgG Human E 0.2 mg CL20100HP 262 Protein C/APC (Calcium Dependent) Purified CaC-11 mouse IgG1 Human E, WB 200 μg CL2769AP 259 Protein S Purified sheep IgG Human IP, E, D 10 mg CL20105A 136 Protein S Affinity Purified sheep IgG Human E 0.5 mg CL20105AP 257 Protein S HRPO sheep IgG Human E, WB 0.2 mg CL20105HP 182 Protein S Purified goat IgG Human IP 5 mg CL20106A 136 Protein S HRPO goat IgG Human E, WB 0.2 mg CL20106HP 136 Protein Z Purified sheep IgG Human IP 10 mg CL20107A 154 Protein Z Affinity Purified sheep IgG Human E 0.5 mg CL20107AP 317 Protein Z HRPO sheep IgG Human E 0.2 μg CL20107HP 209 Proteinase Inhibitor 8 Purified anti-pi8k mouse IgG1 Human E, WB 200 μg CL2770AP 259 Prothrombin (Calcium Dependent) Purified CaPro-20 mouse IgG1 Human E, WB 200 μg CL2761AP 259 Prothrombin (Calcium Dependent) Biotin CaPro-20 mouse IgG1 Human E, WB 100 μg CL2761B 199 Prothrombin (Factor II) Purified sheep IgG Human IP, D, WB 10 mg CL20110A 101 Prothrombin (Factor II) Affinity Purified sheep IgG Human E, WB 0.5 mg CL20110AP 209 Prothrombin (Factor II) HRPO sheep IgG Human E, WB 0.2 mg CL20110HP 209 Prothrombin (Factor II), Fragment I Affinity Purified sheep IgG Human E 0.5 mg CL20111AP 262 Prothrombin (Factor II), Fragment II Affinity Purified sheep IgG Human E, WB 0.5 mg CL20112AP 249 rpro-kallikrein 5 (hk5) Serum rabbit IgG Human WB 200 μl CLAS ß2 Glycoprotein-I (Apolipoprotein-H) Purified goat IgG Human IP 5 mg CL20021A 154 ß2 Glycoprotein-I (Apolipoprotein-H) Affinity Purified goat IgG Human E, WB 0.5 mg CL20021AP 249 ß2 Glycoprotein-I (Apolipoprotein-H) HRPO goat IgG Human E 0.2 mg CL20021HP 199 Thrombin Purified sheep IgG Bovine IP 10 mg CL20121A-B 115 Thrombin Affinity Purified sheep IgG Bovine E, WB 0.5 mg CL20121AP-B 268 Thrombin HRPO sheep IgG Bovine E, WB 0.2 mg CL20121HP-B 209 Thrombin Purified sheep IgG Rabbit IP 10 mg CL20121A-RB 125 Thrombin Affinity Purified sheep IgG Rabbit E, WB 0.5 mg CL20121AP-RB 275 Thrombin HRPO sheep IgG Rabbit E, WB 0.2 mg CL20121HP-RB 214 Thrombin (Factor IIa) Affinity Purified sheep IgG Human E, WB 0.5 mg CL20119AP 268 Thrombin (Factor IIa) Purified sheep IgG Human IP, N, WB 10 mg CL20120A 115 Thrombin (Factor IIa) Biotin sheep IgG Human WB 0.1 mg CL20120B 209 Thrombin (Factor IIa) FITC sheep IgG Human IF 0.1 mg CL20120F 209 Thrombin (Factor IIa) HRPO sheep IgG Human E, WB 0.2 mg CL20120HP 209 Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Purified sheep IgG Human D 10 mg CL20125A 136 Affinity Purified sheep IgG Human E 0.5 mg CL20125AP 262 HRPO sheep IgG Human E 0.2mg CL20125HP 209 Tissue Factor Purified sheep IgG Human WB, E 10 mg CL20150A 154 Tissue Factor HRPO sheep IgG Human E 0.2 mg CL20150HP 182 Legend for Applications: AC=Affinity Chromatography AGG=Agglutination C=Cryostat Sections CT=Cytotoxicity Assay D=Depletion E=ELISA EC=ELISA (Capture) ED=ELISA (Detection) F=Flow Cytometry FN=Functional Assay IC=Immunocytochemistry ID=Immunodiffusion IF=Immunofluorescence IP=Immunoprecipitation IV=In vivo Assay N=Neutralization P=Paraffin-Embedded Sections R=RIA WB=Western Blotting HAEMOSTASIS ANTIBODIES FROM CEDARLANE 7

8 (continued) Tissue Factor Biotin 320 mouse IgG2a Human E 0.1 mg CL20151B 209 Tissue Factor FITC 320 mouse IgG2a Human F 0.1 mg CL20151F 209 Tissue Factor Pathway Inhibitor Purified sheep IgG Human WB 10 mg CL20155A 203 Tissue Plasminogen Activator (tpa) Purified sheep IgG Human IP, D 10 mg CL20115A 136 Tissue Plasminogen Activator (tpa) Affinity Purified sheep IgG Human E 0.5 mg CL20115AP 257 Tissue Plasminogen Activator (tpa) Biotin sheep IgG Human E 0.1 mg CL20115B 209 Tissue Plasminogen Activator (tpa) FITC sheep IgG Human IF 0.1 mg CL20115F 209 Tissue Plasminogen Activator (tpa) HRPO sheep IgG Human E 0.2 mg CL20115HP 209 Vimentin Purified sheep IgG Human IP, E 10 mg CL20165A 136 Vimentin Affinity Purified sheep IgG Human C, WB, E 0.5 mg CL20165AP 262 Vimentin HRPO (Affinity Pur) sheep IgG Human E, WB 0.1 mg CL20165APHP 194 Vimentin Purified VI-01 mouse IgM Mammal WB, IC 0.1 mg CLX138AP 211 Vimentin Dyomics 547 VI-01 mouse IgM Mammal WB, IC 0.1 mg CLX138DY Vimentin Purified VI-RE/1 mouse IgG1 Human F, WB. IC, E 0.1 mg CLX184AP 211 Vimentin PE VI-RE/1 mouse IgG1 Human F, WB. IC, E 0.1 mg CLX184PE 277 Vimentin Purified VI-10 mouse IgM Human IP, WB, P, IC, 0.1 mg CLX247AP 211 Vimentin DY547 VI-10 mouse IgM Human IP, WB, P, IC, 0.1 mg CLX247DY Vitronectin (S Protein) Purified sheep IgG Human IP, ID, IF 10 mg CL20170A 136 Vitronectin (S Protein) Affinity Purified sheep IgG Human E 0.5 mg CL20170AP 262 Vitronectin (S Protein) HRPO (Affinity Pur) sheep IgG Human E 0.1 mg CL20170APHP 182 von Willebrand Factor Purified sheep IgG Canine IP 5 mg CL20176A-C 154 von Willebrand Factor HRPO sheep IgG Canine WB 0.2 mg CL20176HP-C 182 von Willebrand Factor Purified goat IgG Human IP, E, D, C, P 2.5 mg CL20175A 108 von Willebrand Factor Affinity Purified goat IgG Human E, C, P, WB 0.5 mg CL20175AP 249 von Willebrand Factor Biotin goat IgG Human E, WB 0.1 mg CL20175B 182 von Willebrand Factor FITC goat IgG Human IF 0.1 mg CL20175F 182 von Willebrand Factor HRPO goat IgG Human E, WB 0.15 mg CL20175HP 182 von Willebrand Factor Purified sheep IgG Rat/Mouse IP, E, C 5 mg CL20176A-R 154 von Willebrand Factor HRPO sheep IgG Rat/Mouse WB 0.2 mg CL20176HP-R 182 Legend for Applications: AC=Affinity Chromatography AGG=Agglutination C=Cryostat Sections CT=Cytotoxicity Assay D=Depletion E=ELISA EC=ELISA (Capture) ED=ELISA (Detection) F=Flow Cytometry FN=Functional Assay IC=Immunocytochemistry ID=Immunodiffusion IF=Immunofluorescence IP=Immunoprecipitation IV=In vivo Assay N=Neutralization P=Paraffin-Embedded Sections R=RIA WB=Western Blotting Negative Controls for Haemostasis Negative Control Format Size Cat # Price $ Goat IgG (non-immune) Purified 10 mg CLGT00 42 Goat IgG (non-immune) FITC 0.1 mg CLGT01 99 Goat IgG (non-immune) Biotin 0.1 mg CLGT15 99 Rabbit IgG (non-immune) Purified 10 mg CLRB00 42 Sheep IgG (non-immune) Purified 20 mg CLSP00 42 Sheep IgG (non-immune) FITC 0.1 mg CLSP02 99 Sheep IgG (non-immune) Biotin 0.1 mg CLSP HAEMOSTASIS ANTIBODIES FROM CEDARLANE

9 Species Cross Determined by Immunodiffusion Each antibody listed has been tested for reactivity to the plasma of a variety of species. The double immunodiffusion (DID) technique used tests the ability of an antibody to precipitate an antigen in an agarose gel. The DID technique has limitations and a negative result should not necessarily be interpreted as non-recognition of an antigen. A number of antibodies may react with fewer recognition sites on the antigen of another species and be unable to form an insoluble immune complex (this is observed with many monoclonal antibodies). An antibody may demonstrate a greater spectrum of cross reactivity in applications such as immunoblotting and immunohistochemistry where antigen precipitation is not required. Below are examples of typical results seen in historical lots. See product insert for lot specific testing results. Negative Controls for Haemostasis (+++ strong positive, + weak positive, negative, NT not tested) Cat # Antibodies to: Human Rat Mouse Rabbit Canine Feline Porcine Bovine CL20025A-B Bovine Factor V +++ CL20121A-B Bovine Thrombin +++ CL20000A Human alpha1-antitrypsin CL20001A Human alpha1-antitrypsin CL20005A Human alpha2-antiplasmin +++ CL20015A Human Antithrombin III +++ CL20016A Human Antithrombin III CL20023A Human C1-Inhibitor +++ CL20022A Human C1s +++ CL20040A Human Factor IX CL20025A Human Factor V CL20045A Human Factor X CL20050A Human Factor XI +++ CL20055A Human Factor XII +++ CL20056A Human Factor XIII (A subunit) CL20057A Human Factor XIII Purified CL20065A Human Fibrin fragment E +++ NT CL20067A Human Fibrinogen CL20070A Human Heparin Cofactor-II CL20085A Human Plasminogen CL20090A Human Prekallikrein NT ++ CL20095A Human Protein C +++ CL20096A Human Protein C +++ CL20100A Human Protein C Inhibitor CL20105A Human Protein S +++ CL20106A Human Protein S CL20110A Human Prothrombin (Factor II) NT CL20120A Human Thrombin (Factor IIa) CL20125A Human Thrombin Activatable Fibrinolysis I +++ CL20170A Human Vitronectin +++ CL20175A Human von Willebrand Factor CL20010A Human β2macroglobulin CL20021A Human β2glycoprotein-i (Apo-H) CL20067A-RB Rabbit Fibrinogen NT CL20121A-RB Rabbit Thrombin +++ Paired and ELISA HAEMOSTASIS ANTIBODIES FROM CEDARLANE 9

10 Paired and ELISA Paired and ELISA Cedarlane s paired antibody sets are formulated for researchers to develop their own ELISAs in-house. Each paired antibody set comes with an affinity purified capture antibody and an HRPO conjugated detection antibody along with a step-by-step protocol for sandwich ELISA, as well as recipes for all necessary buffers. Alternatively, our buffer pack can be purchased separately (see page 11). Paired Antibodies (E=ELISA) Specificity Format Species alpha-1 Antitrypsin Aff.Pur./HRPO Human E 2 vials CL20002K 340 Antithrombin III Aff.Pur./HRPO Human E 2 vials CL20016K 340 Antithrombin ATIII Aff.Pur./HRPO Mouse E 2 vials CL20017K 340 APC-Alpha 1 Antitrypsin Complex Aff.Pur./HRPO Human E 2 vials CL20003K 384 APC-Protein C Inhibitor Complex Aff.Pur./HRPO Human E 2 vials CL20201K 384 Beta2-Glycoprotein-I Aff.Pur./HRPO Human E 2 vials CL20220K 365 Factor IX Aff.Pur./HRPO Human E 2 vials CL20041K 340 Factor IX Aff.Pur./HRPO Canine E 2 vials CL20044K 395 Factor V Aff.Pur./HRPO Human E 2 vials CL20225K 340 Factor VII Aff.Pur./HRPO Human E 2 vials CL20030K 384 Factor VIII Aff.Pur./HRPO Canine E 2 vials CL20036K 481 Factor VIII:C Aff.Pur./HRPO Human E 2 vials CL20035K 481 Factor X Aff.Pur./HRPO Mouse E 2 vials CL20049K 340 Factor X Aff.Pur./HRPO Human E 2 vials CL20245K 340 Factor XI Aff.Pur./HRPO Human E 2 vials CL20250K 340 Factor XII Aff.Pur./HRPO Human E 2 vials CL20055K 384 Factor XIII Aff.Pur./HRPO Human E 2 vials CL20057K 384 Fibrinogen Aff.Pur./HRPO Mouse E 2 vials CL20048K 365 Fibrinogen Aff.Pur./HRPO Human E 2 vials CL20067K 340 Fibrinogen Aff.Pur./HRPO Rabbit E 2 vials CL20067K-RB 506 Heparin Cofactor II Aff.Pur./HRPO Human E 2 vials CL20071K 340 Kininogen Aff.Pur./HRPO Human E 2 vials CL20027K 395 Plasminogen Aff.Pur./HRPO Human E 2 vials CL20086K 340 Plasminogen Aff.Pur./HRPO Mouse E 2 vials CL20087K 340 Prekallikrein Aff.Pur./HRPO Human E 2 vials CL20090K 395 Protein C Aff.Pur./HRPO Human E 2 vials CL20096K 340 Protein C Inhibitor Aff.Pur./HRPO Human E 2 vials CL20295K 340 Protein S Aff.Pur./HRPO Human E 2 vials CL20106K 340 Prothrombin Aff.Pur./HRPO Human E 2 vials CL20111K 340 Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Aff.Pur./HRPO Human E 2 vials CL20125K 458 Thrombin Activatable Fibrinolysis Inhibitor (TAFI), Complete ELISA ELISA Kit Human E Kit CL20126E 458 Thrombin-Antithrombin Complex Aff.Pur./HRPO Human E 2 vials CL20018K 415 Thrombin-Heparin Cofactor II Complex Aff.Pur./HRPO Human E 2 vials CL20200K 415 Tissue Plasminogen Activator Aff.Pur./HRPO Human E 2 vials CL20116K 340 von Willebrand Factor Aff.Pur./HRPO Human E 2 vials CL20403K HAEMOSTASIS ANTIBODIES FROM CEDARLANE

11 Species Cross- Using Paired for ELISA The values given are the reactivities relative to human reference plasma (W.H.O. traceable) when the test plasmas are assayed under the conditions specified in the protocol for each assay. Plasmas with reactivities of less than 1% are listed as negative. Optimization of the assay conditions may be required to efficiently utilize these assays for the measurement of these analytes in animal plasmas. Below are examples of typical results seen in historical lots. Species Recognition Cat # Analyte Human Rat Mouse Rabbit Canine Feline Porcine Bovine CL20016K Antithrombin antigen 100% 13% 25% neg 9% 21% 4% neg CL20035K Factor VIII antigen 100% neg neg neg neg NT neg neg CL20041K Factor IX antigen 100% neg neg 14% 1% NT neg neg CL20067K Fibrinogen antigen 100% 66% 18% NT 30% 44% 14% neg CL20067K-RB Rabbit Fibrinogen antigen 47% 19% 19% 100% 28% 26% 14% 17% CL20086K Plasminogen antigen 100% 4% 5% 11% 33% NT NT 3% CL20096K Protein C antigen 100% neg neg neg neg neg neg neg CL20106K Protein S antigen 100% neg neg 2% neg 2% neg neg CL20111K Prothrombin antigen 100% neg neg 17% neg neg neg neg CL20125K Thrombin Activatable Fibrinolysis Inhibitor 100% 3% 7% neg neg NT 5% neg CL20245K Factor X antigen 100% neg 1% neg 1% NT neg neg CL20403K von Willebrand Factor antigen 100% 15% 6% 34% 31% NT 31% neg Paired and ELISA Cedarlane s Buffer Pack Description Size Cat # Price $ Buffer Pack for Haemostasis Paired Antibodies Reagents sufficient for 5 x 96 well assays CL HAEMOSTASIS ANTIBODIES FROM CEDARLANE 11

12 For more information, contact: In Canada 4410 Paletta Court, Burlington, ON L7L 5R2 Toll Free: T: (289) , F: (289) In USA 1210 Turrentine Street, Burlington, NC Toll Free: T: (336) , F: (336) Pricing listed is in USD and subject to change without notice.