Product Datasheet. 5-HT3A Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 3

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1 Product Datasheet 5-HT3A Antibody NB Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 3 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 8/7/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB HT3A Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer Target Molecular Weight 0.1 mg 0.5 mg/ml Store at -20C. Avoid freeze-thaw cycles. Polyclonal 0.02% Sodium Azide IgG Immunogen affinity purified Tris (ph 7.3), 0.5% BSA 55 kda Page 1 of 4 v.20.1 Updated 8/7/2018 Product Description Host Goat Gene ID 3359 Gene Symbol Species Specificity/Sensitivity Immunogen Product Application Details Applications HTR3A Human, Rat This antibody is expected to recognise all three reported isoforms (NP_ ; NP_ ; NP_ ). Peptide with sequence C-GPQDFEKSPRDR corresponding to internal region according to NP_ , NP_ , NP_ Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA Recommended Dilutions Western Blot ug/ml, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry-Paraffin 3.75 ug/ml, Immunohistochemistry- Frozen 1:10-1:500, Peptide ELISA Detection limit 1: Application Notes ICC/IF successful usage for rat neurons is reported in scientific literature (PMID: ). WB: Approx. 55 kda band observed in human colon lysates (calculated MW of 55.3 kda band according to NP_ An additional band of unknown identity was also consistently observed at 200 kda. This band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Small Intestine.

3 Images Western Blot: 5-HT3A Antibody [NB ] - Staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Page 2 of 4 v.20.1 Updated 8/7/2018 Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB ] - staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer ph 6, AP-staining. Immunohistochemistry-Frozen: 5-HT3A Antibody [NB ] - Analysis of 5-HT3A in C57BL/6 mouse retina section using anti-5-ht3a antibody. Image from verified customer review. Publications Dremencov E, Weizmann Y, Kinor N et al. Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response. Curr Drug Targets 2006 Feb [PMID: ] Lee SO, Abdelrahim M, Yoon K et al. Inactivation of the orphan nuclear receptor TR3/Nur77 inhibits pancreatic cancer cell and tumor growth. Cancer Res Sep 1 [PMID: ] (WB) Details: HTR3A (IMG-30127) and SP1 (IMG-3860). WB: Endogenous p300 was immunoprecipitated with anti-p300 antibodies and the resulting immunoprecipitates were analyzed by WB using the Sp1 antibody (IMG-3860), Fig 6C. WB: Nuclear extracts from Panc1 cells were subject to WB with the TR3 and Sp1 antibody, Fig 5D. WB: Panc1 cells treated with Sp1 sirna, Fig 4D. WB: Panc1 cells treated with TR3 sirna, Fig 1D.Note: The Sp1 and TR3 antibodies was sirna validated by WB in Panc1 cells, Figs 1D & 4D, respectively. Lu CL, Hsieh JC, Dun NJ et al. Estrogen rapidly modulates 5-hydroxytrytophan-induced visceral hypersensitivity via GPR30 in rats. Gastroenterology 2009 Sep [PMID: ]

4 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NBL HAF109 HAF017 NBP mg 5-HT3A Overexpression Lysate (Native) Donkey anti-goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat IgG F(ab)2 Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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