Roche Sequencing Solutions CHANGING SCIENCE CHANGING LIVES

Transcription

1 Roche Sequencing Solutions CHANGING SCIENCE CHANGING LIVES

2 Roche Sequencing Solutions Roche is helping to shape the future of personalized medicine by integrating best-in-class sequencing technologies into efficient workflows that deliver robust molecular profiles

3 AVENIO ctdna Analysis Kits The complete NGS liquid biopsy solution Day 1 Day 2 Day 3 Day 4 Day 5 AVENIO cfdna Isolation Kit AVENIO ctdna Library Prep Kit AVENIO ctdna Enrichment Kit Illumina NextSeq 500/550 AVENIO ctdna Analysis Software AVENIO Post- Hybridization Kit cfdna Isolation Library Generation Target Enrichment Sequencing Analysis & Report NextSeq 500/550 instruments and associated sequencing reagents are manufactured and sold by lllumina and are not supplied by Roche. Targeted Panel Expanded Panel Surveillance Panel NCCN Guidelines Clinical Trials Tracking Genes genes Size 80kb 200kb 200kb Mutation Classes SNVs, CNVs, Indels, Fusions Data on file / Research Use Only. Not for diagnostic use. Advantages Guideline-driven and emerging biomarkers Three NGS liquid biopsy assays All four mutation classes in a single panel Streamlined, end-to-end workflow 3

4 Roche Cell-Free DNA Collection Tube.

5 Roche Sample Prep DNA Isolation cfdna Collection Tubes, cfna Buffer Kits, MagNA Pure 24 and MagNA Pure 96 MagNA Pure 24 System cfna Pure Buffer Set MagNA Pure 96 System Advantages Reliable NA Prepratation Scalable Extraction True walkaway automation 5

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7 Roche KAPA NGS Kits Enzyme Evolution Technology Application / Sample Type gdna Quant and QC Kits HTP/LTP Library Prep Kits HyperPrep Kits HyperPlus Kits RNA HyperPrep Kits mrna HyperPrep Kits RNA HyperPrep Kits with RiboErase Library Prep Kits for Ion Torrent Library Amplifcation Kit Single-Indexed Adapters Pure Beads Library Quantifition Kit Whole-genome Sequencing (human) Whole-genome Sequencing (small genomes) Exome Sequencing Amplicon Sequencing ChIP-Seq Methyl-Seq FFPE DNA Cell-free DNA Whole Transcriptome mrna-seq Non-coding RNA FFPE RNA Targeted RNA-Seq Ion Torrent Sequencing Advantages Reliable concentration and quality information with a single assay Reliable conversion rates and RNA depletion Reliable and sensitive quantification of all sequencingcompetent library molecules 7

8 Target Regions Genomic DNA Prepare with Next-Gen Sequencing Adaptors Library Preparation Hybridization SeqCap EZ Probes (Solution Captture) SeqCap EZ Probes (Solution Captture) Capture & Washing Amplify DNA and Enrichment QC Amplification and QC Sequence DNA on a Next-Gen Sequencer Sequencing

9 Target Enrichment Sequence Capture / HEAT-Seq SeqCap EZ Choice SeqCap EZ Developer Custom Panels SeqCap EZ MedExome System Whole Exome SeqCap RNA System Transcriptome Sequencing SeqCap Epi System DNA Methylation assessment at singlebase resolution Create your design using HEAT-Seq Enrichment System with fast and easy workflow for powerful SNV detection by MIP-Technology Advantages Reduce sequencing costs by pre-capture multiplexing Maximize automation with HyperCap SeqCap EZ HyperCap: Performance. Speed. Automatability. 9

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11 Automation SeqCap EZ HyperCap Workflow enables easy automation Vendor Instrument KAPA Library Prep Kit Hyper Prep Kit Hyper Plus Kit SeqCap EZ v5.1 HyperCap v1.0 Sciclone Perkin Elmer Zephyr NGS Express Beckmann FX Tecan EVO NGS Hamilton STAR NGS Agilent Bravo B Eppendorf epmoption TMX 5075 Refers to methods in development Automation platforms available for use with SeqCap EZ enrichment products. Note: The availability of Standard Solutions is constantly changing. Contact your instrument providers or see KAPA s Automation webpage to obtain the most up-to-date list of available applications. Advantages Speed with streamlined workflow Easily automatable workflow with broad platform compatibility On-site Support and Hotline Service 11

12 cfdna Collection Tubes cfna Buffer Kits MagNA Pure 96 & MagNA Pure Roche KAPA NGS Kits Target Enrichment Automation AVENIO ctdna Analysis Kits Roche Diagnostics Deutschland GmbH Sandhofer Straße Mannheim AVENIO, KAPA, NIMBLEGEN, SEQCAP und MAGNA PURE sind Marken von Roche. NEXTSEQ ist eine Marke von lllumina. Andere Produktnamen und Marken gehören dem jeweiligen Eigentümer. Nur für Forschungszwecke. Nicht für diagnostsche Anwendungen geeignet Roche Diagnostics. Alle Rechte vorbehalten.