DEPARTMENT OF PLANT BREEDING AND GENETICS

Transcription

1 DEPARTMENT OF PLANT BREEDING AND GENETICS Course No. PBG 511 (Principles of Genetics and Cell Biology ) Cr. Hr. 3(2+1) Teacher Dr. D.K.Gothwal Extra chromosomal inheritance Concepts of Eugenics and Epigenetics Genetic disorders and Behavioral genetics Cell, its ultrastructures, differences between eukaryotic and prokaryotic cells Macromolecules of cell Structure and function of cell wall, nuclear membrane and plasma membrane Cellular Organelles nucleus, Cellular Organelles plastids- chloroplasts and chromoplast Practical

2 Demonstration of genetic principles using laboratory organisms Induction and detection of mutations through genetic tests Principles of microscopy: parts of a microscope and their functions, types Phase contrast microscopy Autoradiography and Electron microscopy Differential centrifugation for isolating macroscopic components Preparation and use of different killing and fixation reagents used in cytological studies Preparation of important stains like aceto caramine, aceto orecin and fuelgen stain. DEPARTMENT OF PLANT BREEDING AND GENETICS Course No. PBG 512 (Principles of Plant Breeding) Cr. Hr. 3(2+1) Teacher Dr. M.L.Jakhar Multiline method Population breeding in self-pollinated crops (diallel selective mating approach) Breeding methods in cross pollinated crops; Population breeding-mass selection and ear-to-row methods S1 and S2 progeny testing, progeny selection schemes

3 Recurrent selection schemes for intra and inter- population improvement I Recurrent selection schemes for intra and inter- population improvement II Development of synthetics and composites Hybrid breeding - genetical and physiological basis of heterosis and inbreeding Production of inbreds, breeding approaches for improvement of inbreds, 24 predicting hybrid performance Seed production of hybrid and their parent varieties/inbreds Breeding methods in asexually/clonally propagated crops, clonal selection and apomixes, Male sterility in crop plants and their commercial exploitation Self-incompatibility in crop plants and their commercial exploitation Concept of plant ideotype and its role in crop improvement Practical Analysis of variance (ANOVA) through RBD Analysis of variance (ANOVA) through LSD Estimation of components of variance, heritability and genetic advance Maintenance of experimental records DEPARTMENT OF PLANT BREEDING AND GENETICS Course No. PBG 513 (Molecular Genetics) Cr. Hr. 3(3+0) Teacher Dr. D.K.Gothwal Historical background of molecular genetics Genetic material in organisms- Discovery & introduction Proof for DNA as genetic material Proof for RNA as genetic material Structure of nucleic acid: DNA, B- DNA, Z- DNA etc.

4 Transposable genetic elements : in prokaryotes Transposable genetic elements : in eukaryotes Split genes Overlapping genes Pseudogenes Oncogenes Gene families and clusters Mutation: introduction and types Molecular mechanisms of mutation: tuatomerism, DNA repair and suppression Molecular chaperones & Gene expression Regulation of gene activity in prokaryotes: positive and negative Gene regulation in eukaryotes RNA splicing and editing Genomics and Proteomics Functional, pharmacogenomics and Metagenomics Signal transduction DEPARTMENT OF PLANT BREEDING AND GENETICS Course No. PBG 531 (Mutagenesis and Mutation Breeding) Cr. Hr. 3(2+1) Teacher Dr. Dhirendra Singh

5 Individual plant based mutation analysis and working out effectiveness and efficiency in M3 generation Comparative evaluation of physical and chemical mutagens for creation of variability in the same species Case studies Use of mutagens for creating oligo and polygenic mutations In vitro mutagenesis callus and pollen irradiation Handling of segregating genrations and selection procedures Validation of mutants Mutation breeding for various traits (disease resistance, insect resistance, quality improvement,etc) in different crops Practical

6 Treating plant propagules (seeds) with graded dose of different chemical mutagens, Computing LD50 for different cops of interest Learning combined mutagenic effect, raising the crop for estimating the mutagenic efficiency and effectiveness using data from the literature Study of M1 generation Parameters to be observed Study of M2 generation Parameters to be observed Procedure for detection of mutations for polygenic traits in M2 and M3 generations. Course No. PBG 532 (Heterosis Breeding) Cr. Hr. 3(2+1) Teacher Dr. Dhirendra Singh Problems and prospects; Apomixis in fixing heterosis-concept of single line 24 hybrid Application of biotechnology in heterosis breeding- molecular markers, doubled 25 haploids, somatic hybridization Organellar heterosis and complementation Creation of male sterility through genetic engineering and its exploitation in 27 heterosis Heterosis breeding in wheat and rice Heterosis breeding in cotton

7 Practical Hybrid seed production in fibre crops Hybrid breeding at National and International level Identification of maintainers and restorers (maize, sorghum, bajra, wheat, rice and brassica Identification and characterization of important hybrids in field crops Field visit of hybrid seed production plots DEPARTMENT OF PLANT BREEDING AND GENETICS Course No. PBG 621 (Genetic Engineering) Cr. Hr. 3(2+1) Teacher Dr. K.Ramakrishna

8 Gene cloning in plasmids Agrobacterium plasmids (Ti and Ri) Agrobacterium plasmids (Ti and Ri) Methods of direct gene transfer-elctroporation, microinjction, use of particle gun Construction of genomic libraries: Genomic and cdna library, Strategy, use of phage vector for library PCR and its use Genetic manipulation Practical: Tri-parental mating Tri-parental mating Tri-parental mating Course No. PBG 624 (Breeding Designer Crops) Cr. Hr. 3(2+1)

9 Development of varieties producing targeted compounds Development of varieties producing nutraceuticals and industrial products Success stories in vaccines through biopharming Success stories in modified sugars through biopharming Success stories in gums through biopharming Success stories in starch through biopharming Practical: Techniques of chlorophyll estimation Protein electrophoresis Heat shock proteins Crop genotypes known for heat stress tolerance : demonstration of protein using Protein electrophoresis using sensitive & resistant genotype of the crop.