Novel Developments in Cytometry. Martin Adelmann Marketing Manager Cellular Analysis & Life Science Beckman Coulter EMEAI

Transcription

1 Novel Developments in Cytometry Martin Adelmann Marketing Manager Cellular Analysis & Life Science Beckman Coulter EMEAI 1

2 Innovation in Flow Cytometry Cellios EPICS 1V 2 lasers, 3 colors EPICS Profile II benchtop Cytometer EPICS XL Fully automated clinical cytometer FC500 advanced cytometer Navios/Gallios EPICS II First Laser Analyzer TPS EPICS laser sorter Q-PREP TM introduced 1st automated flow prep EPICS Altra TM Sorter PrepPlus TM Sample preparation for flow cytometry FP1000 cytometry auto prep Beckman Coulter- In flow cytometry from the start- In it it for the duration 2

3 Developing a New Clinical Flow Cytometer Simplify Introduction Automate Innovate Simplification, Automation and Innovation are key drivers for our business 3

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5 The Solution- Why More Colors? Increase information Quality results Improved efficiency Analytics Faster turnaround Consistency More More colors colors to to improve improve accuracy accuracy and and efficiency efficiency 5

6 Gallios Flow Cytometer Available in 3 configurations 6 color 2 Laser (5+1) 8 color 2 Laser (5+3) 10 color 3 Laser (5+3+2) 638nm Analytics 488nm 405nm Modular Modularto tofit fityour yourneeds, needs,today todayand andtomorrow tomorrow 6

7 Laser Design Innovative laser cartridges Picomotor driven beam steering optics Laser control to conserve laser life Allow for easy future integration of new lasers Analytics Design Designfor forlaser laserflexibility flexibility 7

8 Optics Designed for Sensitivity Blue loss Blue power Red loss Red power Violet loss Violet power 638 Analytics Efficient delivery of light Solid state lasers Direct laser light delivery in air Optimized beam steering optics Increased power at flow cell Gallios Cytometer A Cytometer B Inefficient optics design can lose greater than 50% laser power at the flow cell More More power power at at the the flow flow cell cell means means greater greater resolution resolution in in your your applications applications 8

9 Fluorescence Resolution regard the picket fences in our displays as if they bore the sign beware of the data, and not try to play with what is behind them. Practical Flow Cytometry, 4 th edition bit resolution is analogous to increasing the number of pixels in digital photography 1 Megapixel LSR II 262,144 channel display Analytics 4 Megapixel Gallios 1,048,576 channel display More More channels channels of of resolution resolution to to separate separate even even the the dimmest dimmest of of populations populations 9

10 Speed and Yield Patented electronics design incorporates fast, accurate measurement of signals over a wide range of event rates Fast pulse recovery %Yield Gallios Minimize linearity errors Sensitivity of dim particles Adjusts for temperature variability Minimize dead time %Yield Data Rate Gallios Cytometer B Analytics Efficiency Cytometer A Data Rate Accuracy at high event rates Yield Yield enables enables you you to to get get accurate accurate results results in in less less time, time, with with less less cells cells 10

11 Sensitivity and Resolution Analytics Show video Gallios Galliosoptics optics and and electronics electronics deliver deliver superior superior performance performance 8-peak Rainbow beads, Dr Schiemann, Technical University, Munich 11

12 Optical Design for Stability Changes in ambient temperature can affect measurements by more than 25% Mean channel and CV Reproducibility Compensation Quality Customized temperature regulation optimizes the internal settings to maintain optical consistency despite ambient temperature changes Improved Improved stability stability for for accuracy accuracy and and reproducibility reproducibility 12

13 Solastra - Expanding The Universe Of Multicolor Flow Cytometry 13

14 Innovation in Flow Cytometry Tandem Conjugation APC Alexafluor 700 APC Alexafluor 750 PE-Texas Red PE-Cy PE-Cy5 PE-Cy5.5 Beckman Coulter --First First With With Tandems 14

15 Performance Matters: Patented Production Process Native Native State State Unfold Unfold Couple Couple Acceptor Dye Dye Performance Standardization Refold Refold Tandem Video Patented Process Delivers Maximum Brightness 15

16 What Does This Mean? Uncompensated Compensated BCI Vendor A % Comp. (FL5- FL2) Compensation Settings % Comp. (FL2- FL5) Performance Clear Resolution Of Of Low Low Density Antigens 16

17 Performance Matters: Control of Non-Specific Binding Pre Formulation Post Formulation Analytics Monocyte binding of Cyanine dye Unequivocal Identification Of Of Target Cells 17

18 Additional Benefit of Proprietary Blocking Agent SS Lin SS Lin CD14-PC7 CD14-PC7 Without Blocking Agent With Blocking Agent Specificity: Unquestionable Positivity of of Labeled Cells 18

19 Performance Matters: Impact Of Photo-degradation Control Amber Vial + light BCI Vial + light CD19-ECD Performance FL2 log CD3-PC5 CD3-PC7 Patented Packaging Protects Against Photo-degradation 19

20 Performance Matters: Manufacturing Expertise Competitor Conjugates CD3-APC-Alexa Fluor 750 S/N=51.5 S/N=28 CD3-APC-Cy7 CD8-APC Alexa Fluor 700 S/N=36.8 CD8-PerCy5.5 S/N=100 Analytics Beckman Coulter Conjugates CD3-APC-Alexa Fluor 750 CD3-APC-Cy7 CD8-APC Alexa Fluor 700 CD8-PC5.5 S/N=73.5 S/N=83 S/N=159 S/N=282 Consistently Brighter Tandems 20

21 Solastra 5-Color Panel Solutions* B-Cell Kit Kappa Lambda CD19 CD5 CD45 CD20 CD10 CD19 CD38 CD45 T-Cell Kit CD2 CD56 CD7 CD5 CD45 CD8 CD4 CD3 CD45 CD15 CD11b CD16 CD14 CD45 Myeloid Kit HLADR CD56 CD34 CD117 CD45 CD7 CD13 CD34 CD33 CD45 * In Development Aligned with with Bethesda Recommendations 21

22 Bone Marrow: AML AML: CD34+/CD117+/DR+/ with CD7 & CD56 aberrant expression Tube 2 Tube 3 22

23 Kaluza Analysis Software Explore new dimensions in flow analysis 23

24 Challenges of Flow Analysis Today Today, more colors requires more plots, events, protocol complexity and ultimately increased analysis time complex time events plots colors Kaluza software challenges today s solutions with a new vision of what flow cytometry analysis should be Kaluza Analysis Software is under development, not yet available for in vitro diagnostic use. 24

25 Intelligent Workspace Design Simple Intuitive Intelligent Load data directly from Windows Explorer Or double-click like opening a MS Word file Kaluza Kaluzathat works works with with you you not not against against you you 25

26 Multiparametric Analysis Bivariate plotting becomes inadequate when exploring data in more than 4 or 5 dimensions Kaluza Kaluzaa better better way way to to help help visualize multiparameter data data 26

27 Revolutionizing Analysis Instant phenotypic breakdown of data Each phenotype can be used for gating Reduce hundreds of plots into one with the tree plot 27

28 Revolutionizing Analysis Radar plots bring order to chaos by enabling pattern recognition The The Radar Radar plot plot enables enables multi-parametric multi-parametric pattern pattern visualization visualization without without gating gating 28

29 Breaking Down the Barriers with Solutions Speed Multiparametic analysis Complexity Publishing Compatibility Reporting 29