More Capabilities, New Possibilities in Mass Spectrometry

Transcription

1 More Capabilities, New Possibilities in Mass Spectrometry Boston, Oct 14, 2011 Iain Mylchreest, Ph.D San Jose, CA

2 Increasing App s in Life Sciences Research and Applied Markets Proteomics Biomarker discovery Pathway analysis Protein identification PTM s Metabolite ID/Profiling,pK Accelerated drug development and compound assessment BioPharma Biologics discovery and development Expanding Application Base Environmental Water, soil analysis Metabolomics/Lipidomics Comprehensive study of metabolites for disease and therapeutic assessment Food Safety Inspection, nutrition Chemical contaminant, microbial detection Clinical/Toxicology Disease markers Pain management Drugs of abuse 2

3 Dionex: Leading Chromatography Provider Ion Chromatography Systems Leading technology and market presence, especially in environmental and food safety Liquid Chromatography Systems UHPLC-ready across entire platform, comprehensive columns offering Chromatography Data Systems Gold standard performance and complementary to our leading LIMS Complements Our Leading Mass Spectrometry Offering Deep applications expertise for compelling, high productivity, integrated solutions Leading technology and performance across entire portfolio 3

4 Highest Performance LC Portfolio on the Market Dual LC Systems Two systems in one UHPLC Systems 1000 to 1250 Bar Nano LC Solutions Flexibility and ease-of-use Standard Systems Range of detectors Transcend UHPLC Solutions For multiplexed workflows For all applications & configurations, standalone HPLC to MS front end 4

5 The Industry s Leading Portfolio of MS Solutions Non-targeted Analysis ESI-Orbitraps High Resolution Accurate Mass Fast Scanning Hybrid Orbitraps Quantitative Proteomics Metabolomics Bioanalysis Clinical / Toxicology Environmental Food Safety Applied Markets Research Qualitative Proteomics Biomarker Discovery Metabolism Metabolomics Lipidomics PTM Analysis Fast Scanning MS and MSn Triple Quads Targeted Analysis Ion Traps 5

6 Orbitrap: Continually Extending MS Performance Dallas Proof-of-the-principle for the Orbitrap mass analyzer Chicago Orbitrap with external accumulation developed LTQ launch Denver Exactive benchtop LC-Orbitrap launch Denver Orbitrap Velos Pro Orbitrap Elite Q Exactive Long Beach Resolving power >100,000 demonstrated on concept instrument San Antonio First Commercial LTQ Orbitrap launch Philadelphia LTQ Orbitrap Velos Original 1999 Orbitrap patent US 5,886,346 ASMS 2011: The biggest launches in Orbitrap history 6

7 Orbitrap: Unmatched Resolution vs. TOFs 400,000 Protein + Peptide Fragments Peptides Drugs Thermo SCIENTIFIC 300, , ,000 FWHM 2000 m/z 1000 m/z 200 m/z Routine Orbitrap Resolution A B C 7

8 Strengthening Our Leading Position in Mass Spectrometry Revolutionary Q Exactive The Quanfirmation Instrument June 2011 ASMS Launches Extending Ion Trap Leadership: Velos Pro OT Elite Quantum Leap in Orbitrap Performance Metabolite profiling, peptide sequencing, toxicology Sensitivity equal to high performance triple quads Identify, quantify and confirm in one analysis 8 Metabolite ID, peptide sequencing Fastest and most sensitive ion trap on market Ideal hybrid front-end New fragmentation capability Diverse Technology Introductions Proteomics, detailed structural analysis High field Orbitrap most significant advance since first Orbitrap Resolution and scan speed 4X current Orbitrap

9 Velos Pro: Raising the Bar in Ion Trap Performance The Fastest, Most Sensitive Ion Trap in the World Fastest: first ion trap with 66,000 amu/sec. scan speed, 2X improvement New high dynamic range detection system dramatically enhances peptide/small molecule quantitation New Generation II ion optics for improved robustness Unique Trap HCD fragmentation, gives triple quad like fragments for improved molecule structural identification Ultimate in ion trap speed, sensitivity and flexibility 9

10 Orbitrap Elite: The New Performance Standard Unprecedented mass resolution Technology Breakthroughs: New High Field Orbitrap Resolving power of >240,000 4X scan speed improvement Advanced signal processing New 7 Hz HCD capability New secondary detection system for enhanced dynamic range Multiple fragmentation techniques for comprehensive peptide and protein ID; unambiguous small molecule identification Ultimate in top down protein analysis A quantum leap in ultra high resolution and accurate mass analysis 10

11 Benefits of New High Field Orbitrap Analyzer Smaller size delivers 2X improved resolution and scan speed Advanced signal processing gives 2X improved resolution and scan speed With multiple fragmentation capabilities, significantly enhanced performance + High-field Orbitrap Ultra high resolution and fragmentation flexibility for complex analysis 11

12 IgG Analysis: Orbitrap Elite gg e5_ 0u _ 5 _ 5e _tube e s 0_ e g ad e t _ t # T: FTMS + p ESI sid=45.00 Full ms [ ] Orbitrap R Relative Abundance m/z 12

13 Orbitrap Elite: Raw Spectrum zoom-in gg e5_ 0u _ 5 _ 5e _tube e s 0_ e g ad e t _ t # T: FTMS + p ESI sid=45.00 Full ms [ ] Glycoforms Δm = 162 Da Relative Abundance m/z 13

14 14 OT Elite IgG ReSpect Deconvoluted Spectrum

15 Q Exactive: Redefining Qual/Quan Workflows Trace-level metabolites, contaminants, peptides/proteins in complex mixtures Quanfirmation TM : Simultaneously identify, quantify and confirm in one analytical run Resolving power of >140,000 New high performance quadrupole parent ion selection capability New S Lens ion optics 3X sensitivity Targeted & non-targeted screening 3 workflows in one analytical run New data dependent scanning Multiple ion filling capabilities > speed Exceptional MS/MS sensitivity, speed and data quality 15

16 It is a simple instrument! (Makarov) C-trap Quadrupole mass filter Bent Flatapole HCD collision cell Outer electrodes Injection Flatapole Central electrode Z-lens S-Lens assembly Figures and design courtesy J. Rykl, Thermo Fisher Scientific, Capillary

17 Exactive: Speed Resolution Setting: 35,000 Resolution Setting: 70, Relative Abundance Relative Abundance Time (min) Time (min) Peakwidth (FWHM) = 0.8 sec Scans/peak = 21 Peakwidth (FWHM) = 1 sec Scans/peak = 11 17

18 More Possibilities, Powered by Orbitrap Performance Exactive Mass range m/z Mass accuracy: <2ppm Max resolution: 100,000 LTQ Orbitrap XL Mass range m/z Mass accuracy <1ppm Q-Exactive Mass Resolution >100,000 Mass range m/z Mass accuracy: <2ppm Max resolution: 140,000 Orbitrap Velos Pro Mass range m/z Mass accuracy <1ppm Mass Resolution >100,000 Orbitrap Elite Mass range m/z Mass accuracy <1ppm Mass Resolution >240,000 Screening Affordable Flexibility Research 18

19 New Software to Support Workflows Exactfinder 1.0 Integrated HR/AM qual/quan workflow analysis Targeted and non targeted screening MetQuest 1.0 For HR/AM qual/quan metabolic screening Unbiased full-scan data reveals unexpected metabolites Tracefinder 1.1 Automate environmental, food safety & clinical workflows Supports Triple Quad, Ion Trap and Exactive experiments Xcalibur 2.1 Core software control for all Thermo Scientific MS systems Now supports TLX/multiplexing and Turboflow automation New leading edge software for challenging MS workflows 19

20 Novel MS Technologies for Proteins: Applications Growth Drivers Advances in Hardware, Software and Reagents Move Applications to Higher Throughput Structural accuracy at high sensitivity results in complete protein sequence determination including PTMs High sensitivity, accuracy, precision multiple protein quantitation in biological media High sensitivity, accuracy, precision multiple protein quantitation in biological media many experiments in one run Qualitative Quantitative Quantitative /Multiplexed Hardware: VelosPro OrbitrapElite Hardware: TSQVantage QExactive Hardware: VelosPro ObitrapElite Software Software Software Reagents Reagents Reagents Technology improves applications growth follows 20

21 New Proteomics Tools Proteome Discoverer 1.3 Comprehensive mass informatics platform for data mining Integrate biological information from multiple sources SIEVE 2.0 For label-free, semiquantitative, differential expression Expanded to support metabolomic & lipodomic workflows Pinpoint 1.2 Enhances speed and confidence in targeted quantitation. Supports multiple experimental approaches including HRAM Thermo Scientific Pierce Enrichment Kits Enrich, capture and identify proteins, peptides, kinases Peptide Calibration kits and libraries, ActivX kinase probes Easy-nLC 1000 Split-free, nano-flow LC optimized for biomolecule separation Effortless ultra high pressure operation (1000 bar, 15,000 psi) Leading edge tools for proteomics research 21

22 New Enabling Technologies IBI has developed proprietary enabling technology that solves a critical bottleneck in quantitative detection of protein biomarkers. It is a simple, yet powerful, approach. The Mass Spec-Immuno-Assay (MSIA) tip format delivers greater sensitivity, better cost effectiveness and higher throughput compared to other available methods. Pre-MS Proteomic Research Market Sample preparation for Life Science Researchers & Biotech / Pharma Protein Biomarker & Quantitative Immunoassay Research Market Life Science Researchers, Biotech / Pharma & CLIA Testing Clinical Research New tools for quantitative MS-based proteomics Clinical diagnostic research 22

23 MSIA Platform Workflow What we will deliver Targeted Antibodies biofluid (plasma, serum, urine, CSF, tissue lysate) MSIA Pipette Tip Affinity Extraction Proprietary micro-columns pipette tips for enrichment of even Low abundance proteins & variants extracted directly from biofluids Versette ALH Sample Processing Target protein capture, rinse and elution step requires multiple aspiration and dispense cycles enabled by Versette TSQ Vantage MS Mass Spec Analysis Quantitative analysis - Ability to detect different isoforms (splice variants and post translational modifications) using MRM technology & Pinpoint SW. Combining Best in Class enabling technologies for an integrated and automated work flow solution for reproducible, high throughput sample preparation and biomarker quantitation in normal and disease states. 23

24 Summary Dionex acquisition: comprehensive HPLC portfolio complements leading MS technologies Velos Pro Ion Trap: leadership extended with high speed quantitative capabilities High field Orbitrap Elite: with >240,000 resolution, the most significant advancement since first Orbitrap Q Exactive: Revolutionary Quanfirmation: screen, identify, confirm and quantify in one run Workflow based software: comprehensive new suites for all applications 24