Protein Xtra 2018 Protein Workflow Simplified

Transcription

1 Protein Xtra 2018 Protein Workflow Simplified Laboratory Essentials Page 11 SAVE 15% Isolation & Cloning SAVE 10% Page 2 up to 15% Protein Purification Page 6 Sample Preparation SAVE up to 25% Page 3 SAVE 12% Protein Analysis Distributor GE Healthcare Page 8 Prices valid until: 31 December 2018

2 Isolation & Cloning Cell Isolation Percoll Density Gradient Media Percoll is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 g). 10% Ficoll-Paque PLUS Density Gradient Media Ficoll-Paque PLUS is a ready to use, sterile medium for isolation of lymphocytes in high yield from peripheral blood using a simple and rapid centrifugation procedure. It maintains the viability and a representative distribution of B and T lymphocytes. Ficoll-Paque PREMIUM Density Gradient Media Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells. Manufactured under a Quality Management System certified to ISO 13485:2012 Optimised for the isolation of mononuclear cells from human peripheral blood PCR Amplification illustra PuReTaq Ready-To-Go PCR Beads Freeze-dried PCR reagents mix in a bead format Long-term ambient-temperature stable: no freezer space required time: simply add template DNA solution and primers, and cycle More reproducible results: less risk of pipetting errors and contamination 10% DNA Polymerisation Mix dntp Set (20 mm each A, C, G, T) Free from DNase, RNase, and nicking enzyme activity Greater than 99% triphosphate purity (by HPLC) for optimum performance and consistency Buffer free and ready-to-use solutions in multiple formats PERCOLL 250 ML GEHE % $ $ PERCOLL 1 L GEHE % $ $ FICOLL-PAQUE PLUS 6X100ML 6 ITEMS GEHE % $ $ FICOLL-PAQUE PLUS 6X500ML 6 ITEMS GEHE % $1, $ FICOLL-PAQUE PREMIUM 6X100ML 6 ITEMS GEHE % $ $ FICOLL-PAQUE PREMIUM 6X500ML 6 ITEMS GEHE % $1, $1, illustra PURETAQ PCR BEADS 0.2ML 96 TESTS GEHE % $ $ illustra PURETAQ PCR BEADS (0.5ML TUBES) 100 TESTS GEHE % $ $ illustra PURETAQ PCR BEADS (0.2ML TUBES) 96 TESTS GEHE % $ $ MIX DNTP SET 4X10UMOL (20MM A,C,G,T) 1 SET GEHE % $ $ SET DNTP 4X25UMOL (100MM EACH A,C,G,T) 1 SET GEHE % $ $

3 Isolation & Cloning PCR Purification illustra ExoProStar ExoProStar 1-Step PCR and Sequence Reaction Clean-Up reagent provides a simple, singlestep method for removing unincorporated primers and nucleotides prior to downstream analysis. illustra Exonuclease I and Alkaline Phosphatase enzymes are combined in a single, stable mix requiring just one pipetting step to set up the clean-up reaction. Two separate tubes for maximum flexibility Fast 15 min protocol, different reaction sizes Need a one-tube option? Choose ExoProStar S 10% illustra GFX PCR DNA and Gel Band Purification Kit Isolation and concentration of DNA fragments from PCR mixtures, agarose gel bands, and enzyme digests in under 10 min. Flexible elution volume for different DNA concentrations DNA for use in sequencing, PCR, labeling, digestion, and cloning Nucleic Acid Purification illustra NAP Columns Disposable columns prepacked with Sephadex G-25 DNA Grade which require only gravity to run. For rapid and efficient purification of DNA and oligonucleotides 10-mers in less than 15 minutes. Available in three sizes depending on sample volume: 0.5ml, 1ml or 2.5ml Useful for small-scale purification, desalting and buffer exchange 10% illustra EXOPROSTAR 1-STEP 100 REACTIONS 100 TESTS GEHEUS % $ $ illustra EXOPROSTAR 1-STEP 500 REACTIONS 500 TESTS GEHEUS % $ $ illustra EXOPROSTAR 1-STEP 2000 REACTION 2,000 TESTS GEHEUS % $2, $1, illustra EXOPROSTAR S 20 REACTIONS 1 KIT GEHEUS % $30.00 $27.00 illustra EXOPROSTAR S 100 REACTIONS 1 KIT GEHEUS % $ $ illustra EXOPROSTAR S 500 REACTIONS 1 KIT GEHEUS % $ $ KIT PURIFICATION GFX DNA/GEL BAND 100RXN 1 KIT GEHE % $ $ KIT PURIFICATION GFX DNA/GEL BAND 250RXN 1 KIT GEHE % $ $ COLUMN illustra NAP ITEMS GEHE % $ $ COLUMN illustra NAP ITEMS GEHE % $ $ COLUMN illustra NAP-5 50 ITEMS GEHE % $ $

4 Sample Preparation Collection and Storage DNA Sample Collection and Storage FTA cards and FTA Elute cards utilise patented Whatman technology that simplifies collection, transportation, and storage of biological samples and processing of nucleic acids at room temperature. Simply apply your sample to the card, which contains chemicals that lyse cells, denature proteins, and protect nucleic acids from nucleases. The released nucleic acids are entrapped and preserved in the fibres of the matrix. Analytical Sample Preparation 1 Chr Chromatography Paper Whatman cellulose chromatography papers are used worldwide for chromatography. Produced from the highest quality cotton linters with no additives, the papers are of the purity, high quality, and consistency needed. The papers are manufactured and tested specifically for chromatographic techniques and undergo rigorous quality control to ensure uniformity. 1 Chr has a smooth surface, 0.18mm thick with a linear flow rate (water) of 130mm/30min. Good resolution for general analytical separations. GF/F Glass Microfibre Filter Manufactured from pure borosilicate glass. GF/F offers very fine particle retention and good loading capacity and flow rate. 0.7μm nominal particle retention Served as the basis filter for EPA standard for the toxicity characteristic leaching procedure Whatman Uniflo Syringe Filters Now extended to include sterile 13mm and 25mm syringe filters. Whatman Uniflo Syringe Filters are disposable filter units designed to provide clean filtrate from small volumes up to 100ml. They are available in a variety of membrane choices with a polypropylene overmold housing. Whatman Uniflo syringe filters are available with: 13 or 25mm diameters 0.2μm, 0.22μm or 0.45μm pore sizes Sterile or non-sterile options Individual printing on the filter for easy identification Bench top space saving packaging 15% 15% up to 25% FILTER SPARTAN RC 0.45UM 30MM 500 ITEMS WHAT % $1, $1, FILTER SPARTAN RC 0.2UM 30MM 500 ITEMS WHAT % $1, $1, REZIST 30/0.2 PTFE 100/PK 100 ITEMS WHAT % $ $ REZIST 30/0.45 PTFE 100/PK 100 ITEMS WHAT % $ $ REZIST 30/1.0 PTFE 100/PK 100 ITEMS WHAT % $ $ REZIST 50/0.2 PTFE 50/PK 50 ITEMS WHAT % $ $ MUP 0.2UM RC 100/PK 100 ITEMS WHATUN203NPERC 25% $ $ MUP 0.45UM RC 100/PK 100 ITEMS WHATUN203NPURC 25% $ $ MUP 0.2UM PTFE 100/PK 100 ITEMS WHATUN203NPEORG 25% $ $ MUP 0.45UM PTFE 100/PK 100 ITEMS WHATUN203NPUORG 25% $ $ MUP G2 0.2UM RC 100/PK 100 ITEMS WHATGN203NPERC 25% $ $ MUP G2 0.45UM RC 100/PK 100 ITEMS WHATGN203NPURC 25% $ $ MUP G2 0.2UM PTFE 100/PK 100 ITEMS WHATGN203NPEORG 25% $ $ MUP G2 0.45UM PTFE 100/PK 100 ITEMS WHATGN203NPUORG 25% $ $

5 Sample Preparation Puradisc FP 30 Sterile Syringe Filters The FP 30 filter range contains a low protein binding cellulose acetate membrane, for sterile filtration of cell, tissue culture media and samples containing proteins. Available in different pore sizes. up to 25% SPARTAN HPLC Certified Syringe Filters Reproducible filtration for ultra-high performance liquid chromatography (UHPLC) and high performance liquid chromatography (HPLC) sample preparation. SPARTAN filters can also be used in capillary electrophoresis, protein filtration, UV/VIS analysis, and any application requiring a chemically resistant, low proteinbinding, hydrophilic membrane in a polycarbonate housing. Hydrophilic regenerated cellulose membrane exhibits low protein binding Excellent chemical resistance to common aqueous and organic solvents Tested and certified for the absence of UV-absorbing substances at wavelengths of 210 and 254nm with water, methanol, and acetonitrile Documented batch-to-batch quality and consistency, ensuring reproducible results ReZist Syringe and Venting Filters Specifically designed to be resistant to organic solvents. Highly suitable for the clarification of aggressive organic solvents. ReZist 30mm filters can also be used as a venting filter for small vessels. Mini-UniPrep Syringeless Filters Provide a faster and easier way to remove particulates from samples being prepared for (U)HPLC. Allin-one device filtration process allows samples to be processed in one third the time. G2 glass version also available. Compatible with most major autosamplers. FTA CLASSIC CARD 100 ITEMS WHATWB % $ $ INDICATING FTA CLASSIC CARD 100 ITEMS WHATWB % $ $ FTA ELUTE MICRO CARD 100 ITEMS WHATWB % $ $ PAPER CHROMA GRADE 1 CHR 460 X 570MM 100 ITEMS WHAT % $ $ FILTER GLASS MICROFIBRE GF/F 25MM DIAM 100 ITEMS WHAT % $61.20 $52.02 FILTER GLASS MICROFIBRE GF/F 47MM DIAM 100 ITEMS WHAT % $ $ FILTER GLASS MICROFIBRE GF/F 55MM DIAM 100 ITEMS WHAT % $ $ FILTER SYRINGE CA 0.45UM 30MM STERILE 50 ITEMS WHAT % $ $ FILTER SYRINGE CA 0.2UM 30MM STERILE 50 ITEMS WHAT % $ $ FP 30/0.2 CA S MLL 50/PK 50 ITEMS WHAT % $ $ FILTER SYRINGE CA 0.8UM 30MM STERILE 50 ITEMS WHAT % $ $ FILTER SYRINGE CA 1.2UM 30MM STERILE 50 ITEMS WHAT % $ $ WHATMAN UNIFLO 13MM 0.22 PVDF 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 13MM 0.45 PVDF 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 25MM 0.22 PVDF 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 25MM 0.45 PVDF 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 13MM 0.22 NYLON 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 13MM 0.45 NYLON 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 25MM 0.22 NYLON 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 25MM 0.45 NYLON 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 13MM 0.22 PTFE 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 13MM 0.45 PTFE 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 25MM 0.22 PTFE 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 25MM 0.45 PTFE 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 13MM 0.22 PES 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 13MM 0.45 PES 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 25MM 0.22 PES 500/PK 500 ITEMS WHAT % $ $ WHATMAN UNIFLO 25MM 0.45 PES 500/PK 500 ITEMS WHAT % $ $

6 Protein Purification NEW ÄKTA SYRINGE FILTER Protein Prep syringe filter for ÄKTA systems 13mm or 30mm diameter 0.2μm or 0.45μm pore size NEW Tips for choosing the right filter Use 13mm diameter filter for sample volumes <10ml Use 0.2μm pore size filter if the particle size of the chromatography resin is <30μm HiPrep Columns Quick and easy scale-up of your protein purification. HiTrap Columns Convenient and reliable columns for fast and easy purifications either alone or connected in series. HiLoad Columns Convenient & reliable columns for preparative SEC. To receive a 10% discount on any size column, please lifescience@bio-strategy.com 6

7 Protein Purification High Resolution SEC Columns High-resolution columns including the new Superdex 200 Increase, Superose 6 Increase, and Superdex 75 Increase columns for polishing and characterisation of biomolecules. Vivaspin Vivaspin spin columns are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Sephadex G-25 in PD-10 Desalting Columns PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation. PROTEIN PREP FILTER FOR ÄKTA SYSTEMS 13MM 0.2UM 150 ITEMS WHAT % $ $ PROTEIN PREP FILTER FOR ÄKTA SYSTEMS 13MM 0.45UM 150 ITEMS WHAT % $ $ PROTEIN PREP FILTER FOR ÄKTA SYSTEMS 30MM 0.2UM 150 ITEMS WHAT % $ $ PROTEIN PREP FILTER FOR ÄKTA SYSTEMS 30MM 0.45UM 150 ITEMS WHAT % $ $ DISPOSABLE PD-10 DESALTING COLUMNS, 8.3ML, MM 30 ITEMS GEHE % $ $ SAMPLE CONCENTRATORS, VIVASPIN 500, 10KD 25 ITEMS GEHE % $ $ SAMPLE CONCENTRATORS, VIVASPIN 500, 100KD 25 ITEMS GEHE % $ $ SAMPLE CONCENTRATORS, VIVASPIN 2, 10KD 25 ITEMS GEHE % $ $ SAMPLE CONCENTRATORS, VIVASPIN 2, 30KD 25 ITEMS GEHE % $ $ SAMPLE CONCENTRATORS, VIVASPIN 20, 10KD 12 ITEMS GEHE % $ $ SAMPLE CONCENTRATORS, VIVASPIN 20, 30KD 12 ITEMS GEHE % $ $ SAMPLE CONCENTRATORS, VIVASPIN 6, 50KD 25 ITEMS GEHE % $ $ SAMPLE CONCENTRATORS, VIVASPIN 6, 100KD 25 ITEMS GEHE % $ $

8 Analysis Western Blotting Detection ECL Western Blotting Detection Reagents: ECL based on horseradish peroxidase (HRP)-conjugated secondary antibodies has become the most commonly used detection method for Western blotting. It is a sensitive detection method, where the light emission is proportional to protein quantity. Minute quantities of proteins can be detected and quantitated. Longer shelf life: 18 month shelf life on ECL Select and Prime products Stability: ECL products are stable and stored at room temp 12% Amersham ECL Western Blotting Detection Reagents Amersham ECL Western blotting detection reagents Product Amersham ECL start Amersham ECL Amersham ECL Prime Amersham ECL Select Amersham ECL Plex Chemiluminescence detection reagent for detection of high to medium abundant proteins For reliable detection of high to medium abundant proteins For quantitative analysis of high to low abundant proteins For quantitative analysis of medium to very low abundant proteins For multiplex protein analysis and quantitation of high to very low protein levels Detection type Chemiluminescence Chemiluminescence Chemiluminescence Chemiluminescence Fluorescence Optimal membrane Amersham Protran Amersham Protran Amersham Hybond P Amersham Protran Amersham Hybond P Amersham Protran Amersham Hybond LFP Amersham Protran Premium Recommended detection Amersham Hyperfilm (X-ray film) or ImageQuant LAS 500 Amersham Hyperfilm (X-ray film) or ImageQuant LAS 500 Amersham Imager 600 series X-ray film is also an option Amersham Imager 600 series X-ray film is also an option Typhoon FLA series Amersham Imager 600RGB Signal duration < 3 h < 2 h < 24 h < 2 h > 3 months Typical applications Routine protein detection High expressed proteins e g house keeping proteins Tagged proteins Routine protein detection Protein confirmation Overexpressed proteins Tagged proteins Protein quantitation Protein confirmation Protein regulation and expression analysis Protein quantitation Protein confirmation Protein regulation and expression analysis Multiplex analysis Normalization to housekeeping protein Analysis of post translational modifications and isoforms 10 µg 320 ng 5 µg 40 ng 5 µg 20 ng 1.2 µg 5 ng 10 µg 5 ng Typical results WB ERK ½: LOD 320 ng; DR WB ERK ½; LOD: 40 ng; DR: 2.1 WB ERK ½; LOD: 20 ng; DR: 2.4 WB ERK ½; LOD: 5 ng; DR: 2.4 WB ERK ½ (Cy5 on FLA 9500); LOD 10 ng; DR: 3 Description AMERSHAM ECL START, WESTERN BLOTTING DETECTION REAGENTS, (2*200ML) AMERSHAM ECL START, WESTERN BLOTTING DETECTION REAGENTS, (2*100ML) AMERSHAM ECL PRIME, WESTERN BLOTTING DETECTION REAGENT FOR 1000CM2 AMERSHAM ECL SELECT, WESTERN BLOTTING DETECTION REAGENT FOR 1000CM2 Pack Size Code Protein Xtra 1 KIT GEHERPN % $ $ KIT GEHERPN % $ $ KIT GEHERPN % $ $ KIT GEHERPN % $ $

9 Analysis Western Blotting Membranes Amersham Western Blotting Membranes GE Healthcare Life Sciences offers a broad selection of nitrocellulose (NC) and polyvinylidine difluoride (PVDF) Western blotting membranes, with pore sizes ranges to suit your application requirements. Optimised for chemiluminescent and fluorescent detection Excellent protein binding capacity over a wide size range New larger pack sizes reduce your price per blot by up to 30% 12% Amersham Protran NC membranes with high affinity for proteins, great blocking ability, and compatibility with a range of detection methods. Amersham Protran Supported NC membranes supported by an inert polyester nonwoven material, giving the membranes exceptional handling characteristics that allows for stripping and reprobing of the membrane. Amersham Hybond P PVDF membranes with low background and high sensitivity and resolution, for all labeling and detection systems, but particularly recommended for chemiluminescence detection. Amersham Hybond LFP PVDF membranes specifically optimised for fluorescence detection methods. WESTERN BLOTTING MEMBRANES BLOTTING MEMBRANES, AMERSHAM PROTRAN 0.2ΜM NC, 1 ITEM GEHE % $ $ MM 4M BLOTTING MEMBRANES, AMERSHAM PROTRAN 0.45ΜM NC, 1 ITEM GEHE % $ $ MM 4M BLOTTING MEMBRANES, AMERSHAM PROTRAN PREMIUM, 1 ITEM GEHE % $ $ ΜM NC, 300MM 4M BLOTTING MEMBRANES, AMERSHAM PROTRAN PREMIUM, 1 ITEM GEHE % $ $ ΜM NC, 300MM 4M BLOTTING MEMBRANES, AMERSHAM PROTRAN 0.2ΜM NC, 1 ITEM GEHE % $ $ MM 4M BLOTTING MEMBRANES, AMERSHAM PROTRAN 0.45ΜM NC, 1 ITEM GEHE % $ $ MM 4M BLOTTING MEMBRANES, AMERSHAM HYBOND 0.2ΜM PVDF, 1 ITEM GEHE % $ $ MM 4M BLOTTING MEMBRANES, AMERSHAM HYBOND 0.45ΜM PVDF, 1 ITEM GEHE % $ $ MM 4M BLOTTING MEMBRANES, AMERSHAM HYBOND LFP 0.2ΜM 1 ITEM GEHE % $ $ PVDF, 254MM 4M BLOTTING MEMBRANES, PROTRAN BA85, NITROCELLULOSE, 0.45ΜM, Ø=82MM 50 ITEMS WHAT % $ $

10 Analysis Amersham Molecular Weight Markers 12% Amersham ECL Full-Range Rainbow Molecular Weight Markers Ready-to-load markers with sharp, intense bands on gels and blots enable monitoring of electrophoresis progress and convenient confirmation of transfer to blotting membranes Comprised of 10 individually coloured proteins in the molecular weight range of 12,000 to 225,000. Each 250µl pack size is sufficient for use with 50 mini-gels (10x8cm) or 25 large gels (20x20cm) Amersham ECL Secondary Antibody Highly species-specific HRP-conjugated antibodies optimised for use with Amersham ECL Western blotting detection reagents. Whatman Blotting Paper Whatman 3mm Chr Blotting Papers Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind Ensures that no contamination will occur during the transfer step Whatman 3mm Chr considered the industry standard for blotting procedures AMERSHAM MOLECULAR WEIGHT MARKERS FULL-RANGE RAINBOW AMERSHAM MOLECULAR WEIGHT 250 µl GEHERPN800E 12% $ $ MARKERS HIGH-RANGE RAINBOW AMERSHAM MOLECULAR WEIGHT 250 µl GEHERPN756E 12% $ $ MARKERS LONG-RANGE RAINBOW AMERSHAM MOLECULAR WEIGHT 250 µl GEHERPN755E 12% $ $ MARKERS ECL PLEX AMERSHAM FLUORESCENT RAINBOW MARKERS 120 µl GEHERPN850E 12% $ $ WESTERN BLOTTING MARKERS, AMERSHAM ECL DUALVUE 1 KIT GEHERPN810 12% $ $ SECONDARY ANTIBODY ECL MOUSE IGG, HRP-LINKED WHOLE 1 ML GEHENA931-1ML 12% $ $ AB (FROM SHEEP) SECONDARY SHEEP ANTI-MOUSE IGG POLYCLONAL ANTIBODY 1 ML GEHENA9310-1ML 12% $ $ (HRP) SECONDARY DONKEY ANTI-RABBIT IGG POLYCLONAL 1 ML GEHENA934-1ML 12% $ $ ANTIBODY (HRP) SECONDARY SHEEP ANTI-HUMAN IGG POLYCLONAL ANTIBODY 1 ML GEHENA933-1ML 12% $ $ (HRP) SECONDARY GOAT ANTI-RAT IGG POLYCLONAL ANTIBODY (HRP) 1 ML GEHENA935 12% $1, $ ANTI-GST ANTIBODY (HRP) 1 KIT GEHERPN % $ $ CELLULOSE CHROMATOGRAPHY PAPERS, WHATMAN, GRADE 100 ITEMS WHAT % $ $ MM CHR, MM CELLULOSE CHROMATOGRAPHY PAPERS, WHATMAN, GRADE 3MM CHR, MM 100 ITEMS WHAT % $ $

11 1 Laboratory Essentials Benchkote Laboratory Surface Protector Whatman Benchkote is an absorbent lab paper that protects surfaces against hazardous spills. After use, the bench protector sheet is incinerated or disposed of according to local regulations. Benchkote Plus is a thicker, more absorbent paper for demanding or hazardous applications. It can absorb more than 0.75 liters of water per square meter. Grade 556 Drypads Absorbent paper strips for the removal of excess liquids, e.g., from microscope specimens. Grade 2122 Weighing Paper Reliably allows samples to be transferred to scales without adding unwanted substances that could impact analytical results. Whatman Lens Cleaning Tissue High-quality lens cleaning tissue, which is chemically pure and free from silicones and other additives. 15% 15% BENCHKOTE P+SHT 50X60CM 50/PK 50 ITEMS WHAT % $ $ BENCHKOTE SHEET 46X57CM 50/PK 50 ITEMS WHAT % $ $89.90 BENCHKOTE SHEET 46X57CM 100/PK 100 ITEMS WHAT % $ $ BENCHKOTE REEL 46CMX50M 1/PK 50 M WHAT % $ $ DRYPADS 37X100MM 100/PK 100 ITEMS WHAT % $ $ PAPER WEIGHING SHEETS 2122, 150X150MM 500 ITEMS WHAT % $ $ PAPER WEIGHING SHEETS 2122, 100X100MM 500 ITEMS WHAT % $85.99 $ X15CM 25X25/PK LENSTISSUE 25 ITEMS WHAT % $ $ NEW WHATMAN CATALOGUE NOW AVAILABLE Laboratory Filtration Guide - request your copy today! The new Whatman Laboratory Filtration Guide includes all the products to get the results you want in your work! Whether you re in the Food and Beverage, Pharmaceutical, Environmental monitoring or analytical testing markets, this guide has all your filtration needs. Contact our Customer Service team or your local Bio-Strategy Sales Representative today! Distributor GE Healthcare Laboratory filtration Product guide gelifesciences.com 11

12 Prices & discounts in this brochure may be subject to change. Promotions are not valid with any other special offer or discounts and cannot be applied in retrospect. All offers subject to availability and while stocks last. All prices are in NZD excluding GST and local shipping & delivery charges. Promotion code (if stated) must be referenced on your purchase order to receive offer prices. Prices valid until: 31 December 2018 Contact us for more information and to place your orders Bio-Strategy Ltd. T: E: General Electric Company All rights reserved. GE, the GE Monogram, ÄKTA, Amersham, Benchkote, ECL, ECL Select, ExoProStar, FTA, Ficoll, Ficoll-Paque, GFX, HiLoad, HiPrep, HiTrap, Hybond, illustra, MiniUniPrep, Percoll, Protran, Ready-To-Go, ReZist, Sephadex, SPARTAN, Uniflo and Whatman are trademarks of General Electric Company. VivaSpin is trademark of Sartorius Stedim Biotech GmbH. All other third-party trademarks are the property of their respective owners. GE Healthcare reserves the right to make changes in specifications and features shown herein, or discontinue the product described at any time without notice or obligation. Contact your GE Healthcare representative for the most current information. For local office contact information, visit gelifesciences.com/contact GE Healthcare Bio-Sciences Corp., Marlborough, MA *The Polymerase Chain Reaction (PCR) is covered by patents owned by Roche Molecular Systems and F Hoffmann-La Roche Ltd. A license to use the PCR process for certain research and development activities accompanies the purchase of certain reagents from licensed suppliers such as GE Healthcare and affiliates when used in conjunction with an authorised thermal cycler. Phi 29 DNA polymerase and its use for DNA synthesis is covered by US patent numbers 5,854,033. Ready to Go RT-PCR Beads: Use of this product is covered by applicable US patents and corresponding patent claims outside the US held by Roche. The purchase of this product includes a limited, nontransferable immunity from suit under the patent claims for using only this amount of product for the purchaser s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Additional rights for use in non-diagnostic field require a license from Life Technologies. Diagnostic uses require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting Outlicensing at Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, CA 92008; outlicensing@lifetech.co