Product Datasheet. TfR (Transferrin R) Antibody NB Unit Size: 0.1 ml

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1 Product Datasheet TfR (Transferrin R) Antibody NB Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 5 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 8/29/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB TfR (Transferrin R) Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity 0.1 ml mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Polyclonal 0.05% Sodium Azide IgG Buffer PBS (ph 7.2) Target Molecular Weight Product Description Host Immunogen affinity purified 89 kda Rabbit Gene ID 7037 Gene Symbol Species Reactivity Notes Marker Immunogen TFRC Human, Mouse, Rat Rat reactivity reported in customer review Recycling Endosome Marker Page 1 of 4 v.20.1 Updated 8/29/2017 Synthetic peptide corresponding to the residues surrounding Tyrosine 20 of human Transferrin Receptor Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin Western Blot 1:500-1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry- Paraffin 1:50-1:200 Western blot (WB) analysis of Transferrin Receptor (Y20) pab in extracts from 293 cells. Immunohistochemistry (IHC) analyzes of Transferrin Receptor (Y20) pab in paraffin-embedded human brain tissue. Immunocytochemistry/Immunofluorescence was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

3 Images Western Blot: TfR (Transferrin R) Antibody [NB ] - Analysis of TfR1 (Y20) pab at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate Page 2 of 4 v.20.1 Updated 8/29/2017 Immunocytochemistry/Immunofluorescence: Transferrin Receptor Antibody [NB ] - Transferrin receptor antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NB ] - Paraffin-embedded human colorectal carcinoma tissue at 1:50. Western Blot: Transferrin Receptor Antibody [NB ] - Western blot (WB) analysis of Transferrin Receptor (Y20) Antibody in extracts from 293 cells

4 Immunohistochemistry: Transferrin Receptor Antibody [NB ] - Immunohistochemistry (IHC) analyzes of Transferrin Receptor (Y20) Antibody in paraffin-embedded human brain tis Page 3 of 4 v.20.1 Updated 8/29/2017 Publications Miyazawa A, Kuo S, Feinberg SE. Production of progenitor cells from primary human epithelial cell monolayer cultures In Vitro Cell. Dev. Biol. Anim. May :00AM [PMID: ] (Human) Zarruk JG, Berard JL, Passos Dos Santos R et al. Expression of iron homeostasis proteins in the spinal cord in experimental autoimmune encephalomyelitis and their implications for iron accumulation Neurobiol. Dis Feb 25 [PMID: ] (IHC, Mouse) Mishra SK, Funair L, Cressley A et al. High-affinity dkk1 receptor kremen1 is internalized by clathrin-mediated endocytosis PLoS One 2012 [PMID: ] (WB, Human) Peramo A, Feinberg SE, Marcelo CL. Characterization of cultured epithelial cells using a novel technique not requiring enzymatic digestion for subculturing Cell Tissue Bank 2012 Nov 13 [PMID: ] (ICC/IF, Human) Marcelo CL, Peramo A, Ambati A, Feinberg SE. Characterization of a unique technique for culturing primary adult human epithelial progenitor/""stem"" cells BMC Dermatol 2012 Jun 24 [PMID: ] (IHC, Human)

5 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NBP HAF008 NB7156 NBP TfR (Transferrin R) Knockout HeLa Cell Lysate Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody Rabbit IgG Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: