Supplementary Figure 1. (A) Cell proliferative ability and (B) the invasiveness of

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1 LEGEND FOR SUPPLEMENTARY FIGURES Supplementary Figure 1. (A) Cell proliferative ability and (B) the invasiveness of LLC-1, LLC-3, and LLC-5 cell line series were quantified by BrdU assay after 72 h of incubation. The invasiveness of cells was assessed by a cell invasion kit. *Significant difference with the controls, by Student's t test (p<0.05). All experiments were performed independently at least three times. Supplementary Figure 2. Decreased AMOT mrna expression in LLC-5 as determined by (A) microarray and (B) qrt-pcr cells. *Significant difference with the controls, by Student's t test (p<0.05). All experiments were performed independently at least three times. Supplementary Figure 3. (A) Knockdown of AMOT mrna and (B) protein expression by shrna transfection in CL1-0 cells. Inhibition of AMOT increased cancer cell (C) proliferation and (D) migration as determined by WST-1 and wound-healing assay, respectively. (E) Reduced AMOT expression changed the cell morphology in CL1-0 cells. The knockdown of AMOT in CL1-0 cells was performed using AMOT shrna lentiviral particles. The AMOT mrna levels were measured by qrt-pcr. *Significant difference with the controls, by the Student's t test (p<0.05). All experiments were performed independently at least three times.

2 Supplementary Figure 4. Knockdown of AMOT decreased (A) AMOT protein levels, and increased (B) cell proliferation, (C) migration, and (D) EMT in a dose-dependent manner. CL1-0 cells were transfected with various amount of AMOT shrna plasmid (control shrna plasmid, or 3, 6, or 9 μg AMOT shrna plasmid /6 cm dish. The cells were submitted for BrdU incorporation and migration after puromycin selection and pooling. The levels of various proteins were assessed by immunoblotting. *Significant difference with the controls, by the Student's t test (p<0.05). All experiments were performed independently at least three times. Supplementary Figure 5. (A) Ectopic expression of AMOT (p130) changed the cell morphology in CL1-5 cells. The cells were transfected either with pcmv or pcmv-amot (p130) cdna plasmid. Stable clones were created by G418 section. Transfection of AMOT (p130) increased (B) AMOT (p130) level and decreased (C) cell proliferation and (D) migration and led to (E) MET in a dose-dependent manner in CL1-5 cells. CL1-5 cells were transfect various amount of AMOT (p130) cdna plasmid (control plasmid, or 0.75, 1.5, or 3 μg AMOT cdna plasmid /6 cm dish. The cells were submitted for BrdU incorporation and migration after G418 selection and pooling. The expression of AMOT was assessed by immunoblotting. *Significant difference with the

3 controls, by the Student's t test (p<0.05). All experiments were performed independently at least three times. Supplementary Figure 6. The inhibition efficacy of (A) YAP and (B) TAZ sirna in AMOT knockdown CL1-0 cells. Cells were transfected with either non-target, YAP, TAZ or YAP plus TAZ sirna (10 nm) for 48 h. The cells were collected and mrna transcript was assessed by qrt-pcr. *Significant difference with the controls, by the Student's t test (p<0.05). All experiments were performed independently at least three times. Supplementary Figure 7. The effect of YAP and TAZ sirna transfection in LLC-5 cells. LLC-5 cells were transfected with either control, YAP or TAZ sirna (10 nm) for 48 h. The mrna transcripts of YAP and TAZ were determined by qrt-pcr. *Significant difference with the controls, by the Student's t test (p<0.05). All experiments were performed independently at least three times. Supplementary Figure 8. (A) The effect of AMOT shrna in LLC-1 cells. LLC-1 cells were transfected with either control shrna or AMOT shrna plasmid, and the stable clones were selected by puromycin. The AMOT mrna transcript was determined by qrt-pcr. (B) Inhibition of AMOT increased Cyr61 mrna transcripts, whereas (C) over-expression of AMOT decreased Cyr61 level. The Cyr61 levels in AMOT knockdown CL1-0 cells (clones 17 and 18) or AMOT

4 over-expressing CL1-5 cells (clones 4 and 8) were assessed by qrt-pcr. The experiments were performed independently at least three times. *Significant difference with the controls, by the Student's t test (p<0.05). Supplementary Figure 9. Inhibition of YAP and TAZ by sirna decreased (A) YAP and TAZ mrna transcripts in CL1-5 cells. Inhibition of YAP and TAZ reduced (B) cell proliferation, (C) migration, and (D) EMT in LLC-5 and CL1-5 cells. CL1-5 and LLC-5 cells were transfected with either non-target, YAP, or TAZ sirna. After 48 h transfection, cell proliferation was determined by BrdU assay. Cell migration was analyzed as previously described. The expressions of various proteins were assessed by immunoblotting. *Significant difference with the controls, by Student's t test (p<0.05). All experiments were performed independently at least three times. Supplementary Figure 10. The efficiency of Cyr61 sirna transfection in (A) AMOT-knockdown LLC-1 cells. AMOT knockdown LLC-1 cells were transfected with non-target or Cyr61 sirna (10 nm), while the level of Cyr61 and CTGF mrna transcripts were assessed by qrt-pcr. *Significant difference with the controls, by Student's t test (p<0.05). All experiments were performed independently at least three times. Supplementary Figure 11. Inhibition of AMOT increased the (A) cell proliferation,

5 (B) migration and (C) EMT in LLC-1 cells. The proliferation of AMOT-knockdown LLC was assessed by BrdU incorporation analysis after 72 h of incubation. Cell migration was analyzed as previously described. The expressions of various proteins were assessed by immunoblotting. *Significant difference with the controls, by Student's t test (p<0.05). All experiments were performed independently at least three times.

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