Product Datasheet. GRB2 Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 1

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1 Product Datasheet GRB2 Antibody NB Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Publications: 1 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 8/7/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB GRB2 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer 0.1 mg 0.5 mg/ml Store at -20C. Avoid freeze-thaw cycles. Polyclonal 0.02% Sodium Azide IgG Immunogen affinity purified Tris (ph 7.3), 0.5% BSA Page 1 of 4 v.20.1 Updated 8/7/2018 Product Description Host Goat Gene ID 2885 Gene Symbol Species Reactivity Notes Specificity/Sensitivity Immunogen GRB2 Human, Mouse, Rat, Porcine Predicted cross-reactivity based on sequence identity: Canine, Bovine. This antibody is expected to recognize both reported isoforms (NP_ and NP_ ). Peptide with sequence C-PRNYVTPVNRNV corresponding to C-Terminus according to NP_ Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA Western Blot 1-3 ug/ml, Immunohistochemistry ug/ml, Immunohistochemistry-Paraffin ug/ml, Peptide ELISA detection limit 1:32000 WB: Approx. 26 kda band observed in MOLT4 lysates, of human thymus and of human, mouse, rat, porcine brain and spleen lysates (calculated MW of 25.2 kda band according to human NP_ and according to rat NP_ ). IHC-P: Human tonsil stains mantle zone and to a lessser extent germinal centre. Data obtained by previous batch (different goat). Islets of Langerhans of human pancreas is specifically stained. Images Western Blot: GRB2 Antibody [NB ] - (0.05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

3 Immunohistochemistry-Paraffin: GRB2 Antibody [NB ] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody. Page 2 of 4 v.20.1 Updated 8/7/2018 Western Blot: GRB2 Antibody [NB ] - (0.03ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Western Blot: GRB2 Antibody [NB ] - (0.05ug/ml) staining of Human Thymus (A) and Molt4 (B) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Immunohistochemistry-Paraffin: GRB2 Antibody [NB ] - (1ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer ph 6, HRP-staining. Publications Lowenstein EJ, Daly RJ, Batzer AG et al. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell 1992 Aug 7 [PMID: ]

4 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NBP HAF109 HAF017 NBP mg GRB2 Knockout HeLa Cell Lysate Donkey anti-goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat IgG F(ab)2 Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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