Schedule of Accreditation issued by United Kingdom Accreditation Service High Street, Feltham, Middlesex, TW13 4UN, UK

Transcription

1 21-47 High Street, Feltham, Middlesex, TW13 4UN, UK Issue No:034 Issue date: 17 March 2010 Shardlow Business Park London Road Shardlow Derby Derbyshire DE72 2GD Contact: Mr I Johnson Tel: +44 (0) Fax: +44 (0) ijohnson4347@ilsmail.co.uk Website: Testing performed at the above address only DETAIL OF ACCREDITATION BEVERAGES Chemical and Physical Tests Beers, wines and spirits (and foodstuffs) Determination of ethanol content ( % v/v) using gas chromatography with flame ionisation detection (GC- FID) Beers, wines and spirits Alcohol using steam distillation Sorbic acid using gas chromatography by FID Wines Volatile Acid as Acetic Acid using steam distillation Liquids Density/specific gravity/original gravity/present gravity/residual gravity Chloride Ochratoxin A Nitrate/Nitrite using Anton Paar densitometer using Potentiometric Titration by HPLC with Fluorescence Detection by anion exchange chromatography Assessment Manager: LH1 (Testing Single_034) Page 1 of 12

2 ANIMAL FEEDS Microbiological Tests Detection: Salmonella spp and species using selective enrichment in RVS broth and plating on XLD and BGA to meet the requirements of the Animal By Products Regulations (ABPR) 2005 Presumptive Enterobacteriaceae based on BS ISO :2004 in accordance with the Animal By Products Regulations (ABPR) 2005 ENVIRONMENTAL SWABS Microbiological Tests Detection: Listeria spp and species 1) using Buffered Listeria Enrichment Broth and plating on Oxford agar. (CCFRA Technical Methods & 3.2.7) 2) using Half Fraser Broth and Buffered Listeria Enrichment Broth followed by Clearview ELISA. (CCFRA Technical Manual No 43, 5 th Edition 2007, Method 3.2.7) Salmonella spp and species 1) based on ISO 6579:2002 using single selective enrichment in RV Broth and plating on XLD and BGA 2) based on BS EN ISO 6579:2002 Enumeration: Coliforms based on BS ISO 4832:2006 Presumptive Enterobacteriaceae based on BS ISO :2004 Assessment Manager: LH1 (Testing Single_034) Page 2 of 12

3 ENVIRONMENTAL SWABS (cont d) Microbiological Tests (cont d) Enumeration: Escherichia coli Escherichia coli (ß-glucuronidase positive) a) Customer specified method based on BS :1998 b) based on BS ISO :2001 using membrane method Presumptive Enterococci (Group D) Coagulase positive Staphylococci based on BS EN ISO :1999 using latex kit for confirmation Total viable count (mesophilic) based on BS EN ISO 4833:2003 FOOD and FOOD PRODUCTS Chemical and Physical Tests Vitamins: B6 (Pyridoxine) B12 Cyanocobalamin B3 (Nicotinic Acid) B5 (Pantothenic Acid) Folic Acid Biotin Acidity Ash Documented In-house methods using Turbidimetric Microbiological Assay by titrimetry using gravimetric method Ash plus moisture Available carbohydrate Chloride as sodium chloride Cholesterol using titrimetric method using gas chromatography Assessment Manager: LH1 (Testing Single_034) Page 3 of 12

4 FOOD and FOOD PRODUCTS Chemical and Physical Tests Dietary fibre ERH/A w Elemental analysis: 1) using modified Englyst method by gas chromatography 2) AOAC method using Sigma Total Dietary Fibre (AOAC) Kit using Novasina Thermoconstanter Arsenic using hydride generation - atomic Calcium Copper Iron Lead Magnesium Potassium Sodium Zinc using flame atomic using flame atomic using flame atomic using flame atomic using flame atomic using flame atomic absorption using flame atomic using flame atomic Fat 1) Werner-Schmid 2) Rose Gottlieb 3) by NMR Assessment Manager: LH1 (Testing Single_034) Page 4 of 12

5 FOOD and FOOD PRODUCTS Chemical and Physical Tests Elemental analysis: Total Fat Fatty acid profile Hydroxyproline (collagen) Meat content (apparent), energy value and total carbohydrates using Acid Hydrolysis using Soxcap/soxtec extraction using gas chromatography colorimetric, based on BS 4401 Part II by calculation Moisture 1) by gravimetric method 2) Dean and Stark 3) by microwave drying Moisture by vacuum Nitrite Nitrate / Nitrite Nitrogen/protein Phosphate (phosphorous) using gravimetric method using colorimetry by anion exchange chromatography Dumas method using Leco FP using colorimetric method ph value using electrometric method Refractometer solids Saccharin Selenium, Arsenic Mercury Sorbic acid by refractometer using high performance liquid chromatography using atomic fluorescence using atomic fluorescence using high performance liquid chromatography Assessment Manager: LH1 (Testing Single_034) Page 5 of 12

6 FOOD and FOOD PRODUCTS Chemical and Physical Tests Elemental analysis: Starch - native and chemically modified Sugars - total Vitamins A, E Vitamin C using enzyme hydrolysis and titrimetry (Lane and Eynon) using titrimetry (Lane and Eynon) by HPLC using UV/VIS by HPLC using UV/VIS DAIRY PRODUCTS Low level Lactose using Ion Chromatography Cereals, Nuts, Spices and Dried fruit Aflatoxin B 1, B 2, G 1, G 2 Ochratoxin A with HPLC Fluorescence Detection by HPLC Fluorescence Detection Cooked Meat & Meat Products Meat Species using ELISA Uncooked Meat, Meat Products & Milk FOODS AND BEVERAGES Meat Species Inorganic anions - choride - nitrate - sulphate - phosphate Casein/milk protein Total sugars for the purposes of the Food Labelling Regulations 1996 Sulphur dioxide using ELISA using anion exchange chromatography by ELISA method by HPLC i) by distillation ii) by DIHM based on Monier Williams method Black and white pepper Piperine based on BS : 1983, ISO 5564: 1982 Assessment Manager: LH1 (Testing Single_034) Page 6 of 12

7 FOOD and FOOD PRODUCTS Chemical and Physical Tests RAW and COOKED FOODSTUFFS Peanut content STM using Biokits Peanut Assay kit Almond content Hazelnut content Gluten STM using Biokits Almond Assay kit STM using Biokits Hazelnut Assay kit STM using Biokits Gluten Assay kit FISH, CHEESE, WINE Histamine using ELISA FOODS and FOOD PRODUCTS Microbiological Tests Detection: Escherichia coli O157 1) based on BS EN ISO using IMS technique (Dynalbeads) 2) In-house method using selective enrichment in modified EC medium and plating on Sorbitol MacConkey and CT-SMAC agars. Assessment Manager: LH1 (Testing Single_034) Page 7 of 12

8 FOODS and FOOD PRODUCTS Microbiological Tests Detection: Listeria spp and species 1) using Half Fraser Broth and Buffered Listeria Enrichment Broth followed by Clearview ELISA. (CCFRA Technical Manual No 43, 5 th Edition 2007, Method 3.2.7) FOODS and FOOD PRODUCTS (excluding raw meats) Salmonella spp and species 2) using Buffered Listeria Enrichment Broth and plating on Oxford agar (CCFRA Technical Methods & 3.2.7) 3) customer specified method using Buffered Listeria Enrichment Broth and plating on Oxford agar 4) using selective enrichment in Buffered Listeria Enrichment Broth and plating onto Oxford agar. (FDA Bacteriological Analytical Manual January 2003) 1) based on BS EN ISO 6579:2002 2) In-house method based on ISO 6579:2002using single selective enrichment in RV Broth and plating on XLD and BGA Campylobacter spp based on BS EN ISO :2006 Vibrio parahaemolyticus Coliforms Escherichia coli based on Practical Food Microbiology, 3 rd Edition, 2003, Method based on Practical Food Microbiology, 3 rd edition, 2003, Method 4 using LTSB based on Practical Food Microbiology, 3 rd edition, 2003, Method 4 using LTSB Assessment Manager: LH1 (Testing Single_034) Page 8 of 12

9 FOODS and FOOD PRODUCTS (cont d) Microbiological Tests (cont d) Enumeration: Bacillus cereus Presumptive Clostridia - sulphite reducing Presumptive and confirmed Clostridium perfringens Coliforms based on HPA Standard Method F15, issue 1 using PEMBA agar based on BS ISO 15213: based on CCFRA Technical Method ) based on BS ISO 4832:2006 2) based on ISO 4831:2006, by Most Probable Number Presumptive Enterobacteriaceae based on BS ISO :2004 Escherichia coli Escherichia coli (ß-glucuronidase positive) 1) Customer specified method based on BS :1998 2) based on BS ISO : 2001 using membrane method Presumptive Enterococci (Group D) Lactic acid bacteria Listeria spp and species based on BS EN ISO 15214: based on Practical Food Microbiology, 3 rd Edition, 2003, Method 3 Pseudomonas spp based on BS :1996 Spore count (aerobic) Spore count (anaerobic) based on CCFRA Technical Method based on CCFRA Technical Method Assessment Manager: LH1 (Testing Single_034) Page 9 of 12

10 FOODS and FOOD PRODUCTS Microbiological Tests Enumeration: Coagulase positive Staphylococci based on BS EN ISO :1999 using latex kit for confirmation Total viable count (mesophilic) Total viable count (thermophilic) Total viable count (anaerobic mesophilic) Total viable count (anaerobic thermophilic) Yeasts and Moulds based on BS EN ISO 4833:2003 using PCA or MPCA based on CCFRA Technical Method using PCA, MPCA or Dextrose Tryptone agar based on CCFRA Technical Method using Fastidious Anaerobe agar or Reinforced Clostridial agar Pour Plate based on CCFRA Technical Method using Fastidious Anaerobe agar Pour Plate based on ISO 7954:1987 & CCFRA Technical Manual No 43, 5 th Edition 2007, Method 2.1 is in lab s method using RBCA agar MEDICAL EQUIPMENT Microbiological Tests (as appropriate for the product category as detailed in relevant pharmacopoeial monograph) Sterility test Bacterial endotoxins Antibiotic assay Specifications and methods detailed in the British Pharmacopoeia (BP), the European Pharmacopoeia (EP), and the US Pharmacopoeia (USP), supplemented by in house documented procedures ILS USP by direct inoculation by Limulus test using gel clot in accordance with BP/EP/USP by large plate assay Assessment Manager: LH1 (Testing Single_034) Page 10 of 12

11 PHARMACEUTICAL PRODUCTS Microbiological Tests (as appropriate for the product category as detailed in relevant pharmacopoeial monograph) Efficacy of antimicrobial preservatives Specifications and methods detailed in the British Pharmacopoeia (BP), the European Pharmacopoeia (EP), and the US Pharmacopoeia (USP), supplemented by in house documented procedures 1) in accordance with EP/BP 2) in accordance with USP Microbial contamination 1) in accordance with EP/BP 2) in accordance with USP Microbial examination of non-sterile products Sterility Bacterial endotoxins in accordance with harmonised EP/USP method in accordance with EP/BP/USP- Membrane Filtration or Direct Inoculation by Limulus test using gel clot WATER (Potable, Process) Microbiological Tests Enumeration of: Clostridium perfringens and Sulphite Reducing Clostridia Colony Count at 22 C & 37 C Faecal Streptococci Pseudomonas aeruginosa Coliforms & E. coli Documented In House Methods based on The Microbiology of Drinking Water (MDW) Environment Agency using membrane filtration, based on MDW 2004, Part by pour plate, based on MDW 2007, Part using membrane filtration, based on MDW 2002, Part using membrane filtration, based on MDW 2002, Part using Colilert, based on MDW 2002, Part 4 Assessment Manager: LH1 (Testing Single_034) Page 11 of 12

12 WATER (Potable, Process) (cont d) FOODS AND FOODSTUFFS (unspecified) Microbiological Tests Detection of: Legionella spp and of Legionella pneumophila sero group 1 and groups 2-14 Salmonella spp and species Qualitative screening for genetically modified plant material: Round- up- Ready Soya Maximiser Maize 35S Promoter Gene NOS Terminator Gene Documented In House Methods based on The Microbiology of Drinking Water (MDW) Environment Agency Documented In-House Method based on BS :1998; ISO 1731:1998 Documented In House Method based on The Microbiology of Drinking Water 2004 Documented In-House Methods Nos / / using extraction, multiplication and electrophoresis of DNA END Assessment Manager: LH1 (Testing Single_034) Page 12 of 12