Product Data Sheet - TRUEMAB

Transcription

1 Name:LOX mouse monoclonal antibody, clone OTI9G2 (formerly 9G2) Product Data Sheet - TRUEMAB Catalog: TA Components: LOX mouse monoclonal antibody, clone OTI9G2 (formerly 9G2) ( WB positive control also available: 20ug myc-ddk tagged LOX HEK293T over-expression lysate lyophilized in RIPA buffer (LC400843). (Reconstitute into 20ul of 1x SDS sample buffer before loading; load 5ul per lane as WB control or as desired) Amount: 100ul Immunogen: Human recombinant protein fragment corresponding to amino acids of human LOX (NP_002308) produced in HEK293T cell. Host: Mouse Isotype: IgG1 Species Reactivity: Human Guaranteed Applications: WB, IHC, IF, FC Suggested Dilutions: WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100, Concentration: 0.77 mg/ml Buffer: PBS (ph 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Purification: Purified from mouse ascites fluids by affinity chromatography Storage Condition: Shipped at -20C or with ice packs. Upon delivery store at -20C. Dilute in PBS (ph7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. Target Target Name: Homo sapiens lysyl oxidase (LOX), transcript variant 1 Alternative Name: Database Link: Function: MGC NP_ Entrez Gene 4015 Human The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this

2 gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

3 Validation Data HEK293T cells were transfected with the pcmv6- ENTRY control (Left lane) or pcmv6-entry LOX (RC213323, Right lane) cdna for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- LOX. Adenocarcinoma of Human breast tissue using anti-lox Human colon tissue within the normal limits using anti- LOX mouse monoclonal antibody. (Heat-induced 10min,

4 Adenocarcinoma of Human colon tissue using anti-lox Human Kidney tissue within the normal limits using anti- LOX mouse monoclonal antibody. (Heat-induced 10min, Carcinoma of Human kidney tissue using anti-lox

5 Carcinoma of Human lung tissue using anti-lox mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, ph6.0, 100C for 10min, Adenocarcinoma of Human ovary tissue using anti-lox Human pancreas tissue within the normal limits using anti-lox mouse monoclonal antibody. (Heat-induced 10min,

6 Carcinoma of Human pancreas tissue using anti-lox Human thyroid tissue within the normal limits using anti- LOX mouse monoclonal antibody. (Heat-induced 10min, Carcinoma of Human thyroid tissue using anti-lox

7 Adenocarcinoma of Human endometrium tissue using anti-lox mouse monoclonal antibody. (Heat-induced 10min, Human prostate tissue within the normal limits using anti-lox mouse monoclonal antibody. (Heat-induced 10min, Carcinoma of Human prostate tissue using anti-lox

8 Carcinoma of Human bladder tissue using anti-lox Human lymph node tissue within the normal limits using anti-lox mouse monoclonal antibody. (Heat-induced 10min, Human lymphoma tissue using anti-lox mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, ph6.0, 100C for 10min,

9 Anti-LOX mouse monoclonal antibody ( immunofluorescent staining of COS7 cells transiently transfected by pcmv6-entry LOX(RC213323). Flow cytometric Analysis of Hela cells, using anti-lox antibody(,(red), compared to a nonspecific negative control antibody,(blue). Flow cytometric Analysis of Jurkat cells, using anti-lox antibody(,(red), compared to a nonspecific negative control antibody,(blue). * More validation images may be available on our website: