The History of The Binding Site

Transcription

1 Systems ebook

2 Contents 3. History 4. RID Reader 5. Software 7. Calibration methods 9. Technology 11. Complement Assays 13. Immunoglobulin Assays 15. Human Complement Functional Assays 15. Human Protein Assays 16. Human Coagualtion Protein Assays

3 The History of The Binding Site The Digital RID Reader is designed to simplify & speed-up the process of reading precipitin radial immunodiffusion rings. The Binding Site is built on the firm foundations of scientific evidence and a flexibility that allows us to respond to the needs of clinicians and researchers alike. Radial Immunodiffusion is a well-established technique based on the complex formed between antigen and antibody, producing a visible precipitin ring in the gel. Since the early 1970 s, we continually strive to expand our knowledge and understand the challenges facing patients and medical professionals in our industry. The concentration of specific proteins can be determined efficiently and accurately by measuring the precipitin ring diameter using our RID Reader. The Binding Site provides a solution to determine the precipitin ring diameter using the RID Reader or a jewellers eye piece. Using a range of quantitative calibration methods, the RID Reader provides a diameter reading tailored for each assay kit. 53 4

4 With advanced software, the RID Reader is more efficient and customer friendly, meeting all your customer needs. The use of this software allows: Accuracy in reading Efficiency in measurement Confidence in results Software used to enhance your experience Choose from 3 methods of curve calibration to suit your needs. Pre-enter data to generate calibration curve and final sample concentration accordingly. Storage capabilities useful for training and quality control. 5 6

5 Bringing Simplicity to a Proven Science The Binding Site RID Reader provides you with 3 methods of Calibration Curve. This allows you total control and confidence in the concentration generated. The linear curve fit allows reading for RID plates that have been allowed to run to their stated completion time before reading the rings. The point-to-point curve fit is an alternative to the second order curve fit, for non-linear curves. The points of the curve are joined by straight lines. The second order curve fit allows reading when RID plates are to be read prior to their stated completion time, producing a binomial curve. The second order (binomial) curve fit is used to calculate a non-linear curve. 7 8

6 Using advanced technology to bring you defining rings Possessing a built in video camera providing clear imagery of your rings. Enhanced visualisation on-screen. Automatic ring diameter recordings. Accurate and Efficient Accurate measurement of precipitin rings with enlarged imagery. Minimise error and save time associated with manual calculations. Reduces cost with RID reference table by maximising well use. 9 10

7 Complement The Complement system is a complex part of the immune system comprising of numerous proteins which act as a cascade. These assays are efficient as screening tools to detect complement deficiencies. Complement is involved in initiating an inflammatory response and destroying certain bacteria and viruses. Where a complement deficiency is suspected it maybe necessary to test for the specific components of the complement system. C1 Inactivator NL RID Kit RN109.3 Range: mg/l C1q NL RID Kit RN020.3 Range: mg/l** C2 NL RID Kit RN022.3 Range: mg/l C3 NL RID Kit RN023.3 Range: mg/l C4 NL RID Kit RN025.3 Range: mg/l C4 Binding Protein NL RID Kit RN026.3* Range: mg/l C5 NL RID Kit RN027.3 Range: mg/l C6 NL RID Kit RN102.3* Range: mg/l C7 NL RID Kit RN103.3* Range: mg/l C8 NL RID Kit RN089.3* Range: mg/l C9 NL RID Kit RN028.3* Range: mg/l Factor B NL RID Kit RN029.3 Range: mg/l Factor H (β 1H) NL RID Kit RN030.3* Range: mg/l Factor I NL RID Kit RN031.1* Range: 7 70 mg/l ** Diluted sample applied - assay range may be extended using neat sample. Manufacturing date to expiry ranges from 13 to 26 months, unless otherwise * For research use only

8 IgG NL RID Kit RN004.3 Range: mg/l IgG ML RID Kit RL004.3* Range: mg/l Immunoglobulins The Binding Site Immunoglobulin assays measure the amount of IgA, IgG, and IgM in the blood. Immunoglobulins are a critical part of the immune response by recognising and binding to a particular antigen. Determination of individual subclasses is also relevant in assessing primary immunodeficiency or immune responses. IgG1 Subclass SD RID Kit RN106.3 Range: mg/l** IgG2 Subclass SD RID Kit RN107.3 Range: mg/l** IgG3 Subclass SD RID Kit RN108.3 Range: mg/l IgG4 Subclass SD RID Kit RN109.3 Range: mg/l IgG Subclass COMBI SD RID Kit RK021 One plate each of IgG1, IgG2, IgG3 and IgG4 IgA NL RID Kit RN010.3 Range: mg/l IgA ML RID Kit RL010.3* Range: mg/l IgA UL RID Kit GL010.3* Range: mg/l IgA Subclass COMBI kit NL RID Kit RK015 (IgA1 X2 & IgA2 X2) IgA1 Range: mg/l** IgA2 Range: mg/l** Secretory IgA NL RID Kit RN148.3* Range: mg/l IgM NL RID Kit RN012.3 Range: mg/l IgG, IgA, IgM COMBI NL RID Kit One plate each of IgG, IgA, IgM RK002 IgD NL RID Kit RN013.3 Range: mg/l * For research use only. ** Diluted sample applied - assay range may be extended using neat sample. Manufacturing date to expiry ranges from 13 to 26 months, unless otherwise stated

9 Human Complement Functional Assays Functional assays are effective as screening tools to detect complement deficiencies and aid in the monitoring of total complement activity. These assays are based on the haemolysis of red blood cells following activation of the complement system. Human Coagulation Proteins Coagulation proteins are the core components of the coagulation system. Coagulation testing is carried out to determine if an individual has enough coagulation activity to control the blood clotting process and to measure the function or amount of these proteins in the blood. Total Haemolytic Complement Kit RC001.1/RC001.2/RC001.3 Antithrombin III NL RID Kit RN040.3 Range: mg/l Alternative Pathway Haemolytic Complement Kit RC003.1*/RC003.3* Fibrinogen NL RID Kit RN056.3 Range: mg/l Functional C1 Inactivator Kit RC002.3 Plasminogen NL RID Kit RN065.3 Range: mg/l Functional C1 Inactivator COMBI Kit RK019 Two plates of functional C1 Inactivator, One plate of C1 Inactivator Protein C RID Kit GT118.3 Range: mg/l * for research use only Human Proteins Human proteins are useful diagnostic tests for the detection of immune system disorders. Albumin NL RID Kit RN032.3 Range: mg/l** Albumin- ML RID Kit RL032.3* Range: mg/l α 1 Antitrypsin NL RID Kit RN034.3 Range: mg/l β2 Microglobulin EL RID Kit GT043.3 Range: 1 10 mg/l Haptoglobin NL RID Kit RN058.3 Range: mg/l Lysozyme NL RID Kit GT073.3 Range: mg/l Transferrin NL RID Kit RN070.3 Range: mg/l ** Diluted sample applied - assay range may be extended using neat sample. Manufacturing date to expiry ranges from 13 to 26 months, unless otherwise stated

10 Contact us RID Reader, Nanorid and Bindarid are trademarks of The Binding Site Group Ltd, Birmingham UK. Not for use in China. 2018, The Binding Site Group Ltd, Birmingham, UK.