THE NATURAL CHOICE FOR CELL CULTURE

Transcription

1 THE NATURAL CHOICE FOR CELL CULTURE UPM BIOMEDICALS

2 Nature s solution a bio-friendly hydroel extracted from birch and sourced from sustainable and responsibly manaed forests. GrowDex is a ready to use hydroel that mimics the extracellular matrix (ECM), supportin cell rowth and differentiation with consistent results. The versatility of GrowDex has seen it used in numerous applications ranin from: 3D cell culture and oran-on-a-chip models, throuh to dru release studies and 3D printin. GrowDex is ideal for these and other applications due to its unique properties: ANIMAL FREE Xeno-free with no detectable endotoxins, GrowDex is ph neutral and supplied in a user friendly syrine for convenience and ease of use. ROOM TEMPERATURE STABLE Stored, shipped and used at room temperature, this stable hydroel eliminates the need for temperature control, costly transportation and is ideal for use in liquid handlin system for the automation of any cell-based assay. READY TO USE GrowDex truly is a ready-to-use hydroel. No cross-linkin, no elation, no sonication or any other steps required prior to use. Just mix with your media and cells, dispense and incubate. REPRODUCIBLE LOTS Raw material supply, production procedures and quality control checks, to ensure that GrowDex is manufactured reproducibly to the hihest standards. BIOCOMPATIBLE The biocompatible nanofibrillar network allows easy diffusion of small molecules such as nutrients and oxyen supportin the culture of various cell types. TUNABLE VISCOSITY The viscosity stiffness of GrowDex can be tuned by dilution with media. As different cell types thrive in different micro-environments, determinin the optimum conditions for your cells is imperative. Bein ready to use, dilution curves with GrowDex are quick and easy to prepare. MICROSCOPE & IMAGING COMPATIBLE GrowDex is not auto-fluorescent, is compatible with microscope and cell imain systems and can be used for brihtfield, phase contrast or fluorescence imae capture. ONE-STEP CELL RECOVERY GrowDase enzyme allows easy recovery of cells, spheroids or other sample from GrowDex without disruptin or destroyin the structure. VISIT FOR MORE INFORMATION, VIEW THE LATEST PUBLICATIONS AND TO DOWNLOAD OUR APPLICATION NOTES.

3 Culture your cells with confidence Bein a completely xeno-free hydroel, GrowDex enables you to have complete control over the culture environment for you cells. This flexibility has enabled GrowDex to be used in a wide variety of assays culturin cells from many different sources, such as primary hepatocytes for in vitro dru screenin or patient derived cells in a personalized medicine study. Other examples include: 3D oranoid formation Hepatocyte toxicity, induction and metabolism Stem cell proliferation and differentiation, ES, ips & MSCs Tumor cell miration and invasion Neurite outrowth and network formation Patient specific cells and biopsies Human corneal cells Lun adenocarcinoma Osteoblast and chondrocyte Melanoma Co-culture of various cell types Reenerative medicine and tissue enineerin Mix & use Ready to use GrowDex makes cell culture simple. Just mix media, cells and GrowDex toether and dispense. What could be easier! Harvest your cells Add media Add GrowDex Mix Add cells Mix Dispense into culture vessel & incubate at 37 C Recoverin your cells or oranoids post culture is a one step process. Add our GrowDase enzyme to your culture and incubate at 37 C. The enzyme diests the GrowDex leavin your cells in solution for re-platin or downstream processin Spheroids cultured in GrowDex GrowDase added, sample incubated at 37 C GrowDex deraded to lucose, spheroids are in solution Orderin information Visit our webshop at or contact us at rowdex.sales@upm.com. CATALOGUE CODE DESCRIPTION GrowDex 5 ml syrine GrowDex 10 ml syrine Multi-pack GrowDex 3 x 5 ml syrine GrowDex 5 ml syrine + GrowDase 2.5 ml Combo pack GrowDase 2.5 ml, 10 m/ml

4 Not just for cell culture GrowDex is not limited to 2D and 3D plate-based assays, but it has been used successfully in a number of other areas. Here are a few examples to ive you an insiht: Oran-on-a-Chip Models Cell Barrier The thixotropic nature of GrowDex allows the GrowDex at hih concentration can be used to form hydro el to be dispensed into the channels of a barrier preventin cell miration but allowin nutrient, a microfluidic device. GrowDex is able to retain dru or metabolite diffusion. its structure once in place makin it ideal for use with oran-on-a-chip models. 3D Printin The interest in 3D printin, especially in reenerative Dru Delivery Vehicle medicine, has called for a reliable raw material with The biocompatible hydroel can be mixed with reproducible batch quality. GrowDex has both the test dru for use in dru release studies. physical qualities and supply credentials to support this rapidly rowin area. Human embryonic stem cell (hesc) derived neuronal cells Biomeditech, Finland Co-culture human primary hepatocytes and Kupffer cells Admescope, Finland HCE-T Human corneal epithelial cells Experimentica, Finland HepG2 spheroid University of Helsinki, Finland MDA-MB231 (breast cancer) and NIH3T3 (fibroblasts) co-culture Mahatma Gandhi-Doerenkamp Center, India

5 Tunable Ready-to-use Tumor cells Recover cells Innovation with care Automation-friendly fast set up Sustainable Primary cell Biofore Responsible Eco-friendly Reproducible Advanced Transparent Innovative Renewable Simple protocols Natural Reenerative medicine Sterile Natural Animal free 3D cell studies Biocompatible Room-temperature Hydroel Dilute and use

6 UPM Biomedicals Alvar Aallon katu 1 PO Box Helsinki, Finland For Research Use Only. Not for use in dianostic or therapeutic procedures.