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Chastity Morton
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4 Supplementary Figures Figure S1.1. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae MGH

5 Figure S1.2. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0363.

6 Figure S1.3. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0364.

7 Figure S1.4. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0365.

8 Figure S1.5. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0366.

9 Figure S1.6. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0367.

10 Figure S1.7. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0368.

11 Figure S1.8. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0369.

12 Figure S1.9. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0370.

13 Figure S1.10. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0371.

14 Figure S1.11. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0372.

15 Figure S1.12. Ethidium bromide whole cell accumulation assay for isolate K. pneumoniae CFS0373.

A D B E C F Klebsiella pneum oniae M G H 78578 CFS0363

presence of varying concentrations of NMP at 0, 125, 250

Panels D through F, show a different trace curve for the

16 A D B E C F Klebsiella pneum oniae M G H CFS0363 CFS0364 CFS0365 CFS0366 CFS0367 CFS0368 CFS0369 CFS0370 CFS0371 CFS0372 CFS0373 Figure S2. Klebsiella pneumoniae growth curves in MH broth in the presence of varying concentrations of NMP at 0, 125, 250 µg/ml (shown in panels A through C). Panels D through F, show a different trace curve for the reference strain (MGH 78578) and the two most sensitive bacterial isolates to the action of NMP (Klebsiella pneumoniae CFS0364 and CFS0368).

Control M IC C IP M IC C IP + 2 5 0 µ g /m L N M P Figure S3.

presence of ciprofloxacin at the drug concentrations for each isolate in their planktonic state (MIC CIP) and in M9 minimal media with

Biofilms were grown statically at 37 C for 16-18 hours. Biofilm biomass (expressed in OD570) quantified by crystal violet staining.

17 Control M IC C IP M IC C IP µ g /m L N M P Figure S3. Reversal of ciprofloxacin-resistant biofilm in Klebsiella pneumoniae grown in M9 minimal media (control); M9 minimal media in the presence of ciprofloxacin at the drug concentrations for each isolate in their planktonic state (MIC CIP) and in M9 minimal media with 250 µg/ml NMP combined with ciprofloxacin at the drug concentrations for each isolate in their planktonic state (MIC CIP µg/ml). Biofilms were grown statically at 37 C for hours. Biofilm biomass (expressed in OD570) quantified by crystal violet staining. Each isolate was grown in different conditions and was subsequently compared with the control biofilm using ANOVA analysis with Tukey's multiple comparisons - *) P<0.05, **) P<0.01, ***) P<0.001, ****) P<

Control M IC M X F M IC M XF + 250 µ g /m L N M P Figure S4.

presence of moxifloxacin at the drug concentrations for each isolate in their planktonic state (MIC MXF) and in M9 minimal media with

18 Control M IC M X F M IC M XF µ g /m L N M P Figure S4. Reversal of moxifloxacin-resistant biofilm in Klebsiella pneumoniae grown in M9 minimal media (control); M9 minimal media in the presence of moxifloxacin at the drug concentrations for each isolate in their planktonic state (MIC MXF) and in M9 minimal media with 250 µg/ml NMP combined with moxifloxacin at the drug concentrations for each isolate in their planktonic state (MIC MXF µg/ml). Biofilms were grown statically at 37 C for hours. Biofilm biomass (expressed in OD570) quantified by crystal violet staining. Each isolate was grown in different conditions and was subsequently compared with the control biofilm using ANOVA analysis with Tukey's multiple comparisons - *) P<0.05, **) P<0.01, ***) P<0.001, ****) P<