Current Challenges in Protein Expression

Transcription

1 September 24 Session I Membrane Proteins Frank Bernhard Goethe University Frankfurt, DE Peter J.F. Henderson Astbury Centre for Structural Molecular Biology, UK David Drew Imperial College London, UK Andreas Plückthun University of Zürich, CH Christopher G. Tate MRC Laboratory of Molecular Biology, UK Nicola Burgess-Brown SGC, University of Oxford, UK Sponsored lunch time seminars Session II High-throughput Approaches & Mammalian Expression Darren J. Hart European Molecular Biology Laboratory, FR Ray J. Owens Oxford Protein Production Facility, UK Joop van den Heuvel Helmholtz Centre for Infection Research, DE Mark Howarth University of Oxford, UK Session III - Complexes & Baculoviruses Imre Berger European Molecular Biology Laboratory, FR Linda King Brookes University, UK Monique van Oers University of Wageningen, NL Reingard Grabherr Vienna Institute of BioTechnology, AT September 25 Session IV - Current Advances in Expression of Mammalian-derived Proteins & Biologics Bernd Voedisch Novartis Pharma AG, CH Majid Mehtali Vivalis, FR Volker Sandig ProBioGen, DE Bernie Sweeney UCB Pharma, UK Catherine Huntington MedImmune, UK Jörg Brockmann MorphoSys, DE Session V Novartis Tour & Round Table Discussions O R G A N I Z E R S RENÉ ASSENBERG & SABINE GEISSE Novartis Institutes for Biomedical Research, Basel, CH Rene.assenberg@novartis.com Tel: +41(0) R E G I S T R A T I O N - FREE OF CHARGE! On-line, please use the following link: s=1743&lang=2 Includes attendance to the entire MipTec conference Please select the user group meeting Current Challenges in Protein Expression during registration.

2 08:00 08:15 Welcome and Opening Remarks M O N D AY 2 4 TH S E P T E M B E R Session I Membrane Proteins Chair : Myriam Duckely, Novartis Institutes for Biomedical Research, CH 08:15 08:45 Synergies of cell-free expression and nanodisc technology for the production and characterization of membrane proteins Frank Bernhard, Goethe University Frankfurt, DE 08:45 09:25 KEYNOTE LECTURE Membrane transport proteins - the rocky road from gene to molecular mechanism Prof. Peter J.F. Henderson, Astbury Centre for Structural Molecular Biology, UK 09:25 09:55 Benchmarking membrane protein detergent stability to accelerate crystal structures David Drew, Imperial College London, UK 09:55 10:15 Coffee Break 10:15 10:45 Evolving GPCRs for detergent stability and high-yield expression in E. coli Prof. Andreas Plückthun, University of Zürich, CH 10:45 11:15 Engineering GPCRs for improved thermostability and expression to facilitate structure determination Christopher G. Tate, MRC Laboratory of Molecular Biology, UK 11:15 11:45 A high-throughput baculovirus pipeline enabling structural determination of integral membrane proteins Nicola Burgess-Brown, SGC, University of Oxford, UK 11:45 12:45 Lunch

3 M O N D AY 2 4 TH S E P T E M B E R Sponsored Lunch Time Seminars Chair : Rene Assenberg, Novartis Institutes for Biomedical Research, CH 12:45 13:00 Increasing transient protein yields an average of 4- to 5-fold by developing a novel high density 293 culture and transfection system Henry C Chiou Senior Product Manager, Life Technologies Inc, USA 13:00 13:15 Increased CHO Cell Antibody Productivity using the MaxCyte STX Scalable Transfection System Gram Level Quantities of Antibodies from CHO Cells in Less than Two Weeks from as little as 2L of Culture using Scalable Transient Gene Expression (TGE). James Brady Director of Technical Applications, MaxCyte Inc, USA 13:15 13:30 Engineering Genes for Predictable Protein Expression Claes Gustafsson Founder, Chief Commercial Officer, DNA2.0, USA 13:30 14:00 Purification of a recombinant miniature spidroin protein expressed in E. coli Application by means of our novel ÄktaTM pure John Flensburg & Lars Haneskog GE Healthcare, SE

4 M O N D AY 2 4 TH S E P T E M B E R Session II High-throughput Approaches & Mammalian Expression Chair : Imre Berger, European Molecular Biology Laboratory, FR 14:00 14:30 ESPRIT: Library based construct screening for soluble expression of challenging proteins Darren J. Hart, European Molecular Biology Laboratory, FR 14:30 15:00 Streamlining the production of glycoproteins for structural studies Ray J. Owens, Oxford Protein Production Facility, UK 15:00 15:30 Rapid selection of stable animal production cell lines optimized for recombination mediated cassette exchange and high level protein expression Joop van den Heuvel, Helmholtz Centre for Infection Research, DE 15:30 16:00 Building ultra-stable protein assemblies with tools from flesh-eating bacteria Mark Howarth, University of Oxford, UK 16:00 16:20 Coffee Session III Complexes & Baculoviruses Chair : Ian Hunt, Novartis Institutes for Biomedical Research, USA 16:20 17:00 KEYNOTE LECTURE MultiBac: Expanding the research toolbox for multiprotein complexes Imre Berger, European Molecular Biology Laboratory, FR 17:00 17:30 Viruses, vaccines and VLPs; recent developments in the baculovirus expression system Prof. Linda King, Oxford Brookes University, UK 17:30 18:00 Development of a baculovirus virion-free expression system: Exploitation of the essential capsid protein VP80 Monique van Oers, University of Wageningen, NL 18:00 18:30 Insect cells for the production of vaccines: limitations, challenges and needs Reingard Grabherr, Vienna Institute of BioTechnology, AT 18:30 Closing remarks

5 Session IV Current Advances in Expression of Mammalian-Derived Proteins & Biologics Chair : Sabine Geisse, Novartis Institutes for Biomedical Research, CH 08:30 08:40 Opening remarks 08:40 09:10 A new player in the game: The applicability of CAP-T cells to transient protein production for research purposes Bernd Voedisch, Novartis Institutes for Biomedical Research, CH 09:10 09:40 Building on the unique biological properties of embryonic stem cells for the industrial manufacture of vaccines and antibodies Majid Mehtali, Vivalis, FR 09:40 10:10 Fine-tuning glycan composition in CHO Cells - Fucose depletion and beyond Volker Sandig, ProBioGen, DE 10:10 10:30 Coffee Break 10:30 11:00 Optimisation of UCB s transient expression platform Bernie Sweeney, UCB Pharma, UK 11:00 11:30 Influence of expression host cell lines on PK profiles of non-igg recombinant proteins Catherine Huntington, MedImmune, UK 11:30 12:00 IgG production platforms for discovery and development using human cell lines Jörg Brockmann, MorphoSys, DE 12:00 Closing remarks 12:00 14:00 Lunch on your own 14:00 15:15 Novartis Campus Tour T U E S D AY 2 5 TH S E P T E M B E R 15:30 17:00 Round table discussions (Novartis Campus)

6 T U E S D AY 2 5 TH S E P T E M B E R Novartis Tour & Round table Discussions A sign-up list and further info will be made available during the workshop please make sure to register! Program 13:30 Meet outside the congress center 13:35 Take Tram 14 (Messeplatz stop) to Novartis Campus (see maps below) 13:45 Meet at the Novartis Campus Security Desk for badge collection 14:00 Start of the Tour 15:15 For those who did not join the tour: meet at the Novartis Campus Security Desk 15:15 Meet in front of the Canteen: Those wishing to depart will be guided back to the Main Gate Those wishing to attend the discussions will be guided to the cafeteria in the canteen 15:30 Start of the discussions (includes a coffee break, with light refreshments) 17:00 End & Transfer to the main gate Round table discussions Discussions will be led by a panel of experts, who are there to guide the discussions on membrane proteins, mammalian expression & high throughput approaches, protein complexes and biologics. The event will take place in a large space, with several tables dedicated to each topic. The idea is to have it as informal as possible, and to allow people to move easily between discussions if they wish to do so. Some catering will also be provided.

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