CELL LINES TEST TOXICITY, PROTEIN INTERACTIONS, GENE ACTIVATION & INHIBITION. Explore at the cellular level.

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1 CELL LINES Reporter Lines Stable Lines Custom Services TEST TOXICITY, PROTEIN INTERACTIONS, GENE ACTIVATION & INHIBITION Explore at the cellular level

2 USE THESE CELL LINES FOR: BINDING STUDIES DRUG DISCOVERY CO- CULTURE EXPERIMENTS IMMUNOPRECIPITATION REPORTER GENE ASSAYS ANTIBODY SCREENING COMPOUND SCREENING MONITORING SIGNALING PATHWAY ACTIVITY CHARACTERIZING PROTEIN- LIGAND INTERACTIONS AND MORE TYPES OF CELL LINES OFFERED: CELL SIGNALING PATHWAYS ION CHANNELS & TRANSPORTERS PHOSPHODIESTERASES G PROTEIN- COUPLED RECEPTORS IMMUNOTHERAPY ANTIBODY- DEPENDENT CELL- MEDIATED CYTOTOXICITY (ADCC) Table of Contents Ordering Information... Supplementary Products... The Transfection Collection... Uses and Advantages Cell Line Rental 11 Program...

3 Choose from our existing product portfolio OR Customize using our Stable Cell Line Development Service BPS can generate custom stable cell lines for expression verification and functional assays. We can develop high quality cell lines that express your protein at high, homogeneous levels. WHY BPS CELL LINES? Extensive offering - Over 100 cellular assays and corresponding supplements. More than 60 parental cell types available - Including Jurkat, Raji, HEK, Hela, A Large range of back ground cell species available - Including human and other mammalian sources (monkey, CHO, murine, rat, etc.) Produced in house - Get customized, personal support directly from the experts. 1

4 ORDERING INFORMATION Immunotherapy Immunotherapy ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line ADCP Bioassay Effector Cell FcγRIIa (H variant) /NFAT Reporter-Jurkat CD137/NF-κB Reporter - HEK293 Recombinant Cell Line CD137L (4-1BBL) CHO-K1 Recombinant Cell Line CD155 (PVR) - HEK293 Recombinant Cell Line CD155 / TCR Activator - CHO Recombinant Cell line CD27 CHO-K1 Stable Recombiant Cell Line CD40/NF-κB Reporter (Luc) - HEK293 Recombinant Stable Cell Line CD47 - HEK293 Cell Line CD47 CHO-K1 Cell line CTLA4 - HEK293 Recombinant Cell Line Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line GITR / NF-κB Luciferase Reporter (Luc) - Jurkat Cell Line GITR - HEK293 Recombinant Cell Line GITRL CHO-K1 Recombinant Cell Line HEK293 - T-REx Recombinant Cell Line HVEM - CHO Recombinant Cell Line HVEM - HEK293 Recombinant Cell Line HVEM/NF-κB Reporter Jurkat Recombinant Cell Line IDO1 Cell-Based Assay Kit IDO1 Cellular Activity QuickDetect Supplements IDO1 Cellular Activity QuickDetect Supplements IDO1-HEK293 Recombinant Cell line (Human) IDO2 - HEK293 Recombinant Cell Line (Human) IDO2 Cellular Activity QuickDetect Supplements IDO2 Cellular Activity QuickDetect Supplements IL-2-Luciferase Reporter (Luc) - Jurkat Cell Line LAG3 / NFAT Reporter - Jurkat Recombinant Cell Line LIGHT-CHO Recombinant Cell Line NET CHOK1 Recombinant Cell Line NF-κB Luciferase Reporter (Luc) - Jurkat Recombinant Cell Line PD-L1 - CHO Recombinant Cell Line PD-L1 / TCR Activator - CHO Recombinant Cell Line SIRP-α / HEK293 Recombinant Cell Line SIRP-α Jurkat Recombinant Cell Line TCR Activator - CHO Recombinant Cell line TCR Activator - Raji Cell Line TCR Activator / PD-L1 Mammalian Expression Kit TCR Activator / PD-L2 Mammalian Expression Kit TDO Cell-Based Assay Kit TDO Cellular Activity QuickDetect Supplements TDO Cellular Activity QuickDetect Supplements TDO-HEK293 Recombinant Cell line TIGIT - HEK293 Recombinant Cell Line TIGIT / NFAT Reporter - Jurkat Cell Line TLR8/ NFkB Reporter HEK293 Recombinant Cell Line TLR9/ NF-κB Reporter HEK293 Recombinant Cell Line Ion Channel CFTR HEK 293 Recombinant Cell Line Empty vector control - HEK293 Recombinant Cell line herg (Kv11.1) - HEK293 Recombinant Cell line IKCA1 (KCNN4) - HEK293 Recombinant Cell Line NaV1.7 HEK293 Recombinant Cell Line Nav1.8/β2 - HEK293 Recombinant Cell Line TMEM16A-HEK293 Recombinant Cell Line TMEM16B (ANO2) - HEK293 Recombinant Cell line TRPC3-HEK293 Recombinant Cell Line TRPC7-HEK293 Recombinant Cell Line TRPM2-HEK293 Recombinant Cell Line Ion Transporter SLC5A5 HEK293 Recombinant Cell line NFAT Reporter (Luc) Jurkat Recombinant Cell Line Phosphodiesterase Normal Human Peripheral Blood Mononuclear Cells, Frozen OX40 - HEK293 Recombinant Cell Line OX40 / NF-κB Reporter HEK293 Recombinant Cell Line PAI-1 Reporter (Luc) Mv1 Lu Cell Line PD-1 - HEK293 Recombinant Cell Line PD-1 / NFAT Reporter - Jurkat Recombinant Cell Line PD-1:PD-L1/PD-L2 Cell-Based Inhibitor Screening Assay Kit PDE10A-HEK293 Recombinant Cell line PDE1B-HEK293 Recombinant Cell line PDE7A-HEK293 Recombinant Cell Line PDE7A/CRE Reporter - HEK293 Recombinant Cell Line PDE7B-HEK293 Recombinant Cell Line rpde7a-hek293 Recombinant Cell Line sales@bpsbioscience.com bpsbioscience.com 2

5 ORDERING INFORMATION Cell Signaling Pathway AP-1 Reporter HEK293 Recombinant Cell Line (JNK signaling pathway) ARE reporter cell line (Nrf2 antioxidant pathway) CRE/CREB Reporter (Luc) HEK293 Recombinant Cell Line (camp / PKA Signaling Pathway) Dual Luciferase (Firefly-Renilla) Assay System Dual Luciferase (Firefly-Renilla) Assay System Dual Luciferase (Firefly-Renilla) Assay System Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line GAL4 Reporter (Luc)-HEK293 Recombinant Cell Line GITR / NF-κB Luciferase Reporter (Luc) - Jurkat Cell Line Gli Reporter NIH3T3 Recombinant Cell line (Hedgehog Pathway) GR-GAL4 Reporter (Luc)-HEK293 Recombinant Cell Line (Glucocorticoid Receptor Pathway) Hippo Pathway TEAD Reporter - MCF7 Recombinant Cell Line ISRE Reporter HEK293 Recombinant Cell Line (JAK pathway) Myc Reporter (Luc) HCT116 Recombinant Cell Line (Myc Signaling Pathway) NF-κB Reporter (Luc) - CHO-K1 Recombinant Cell Line NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line NF-κB Luciferase Reporter (Luc) - Jurkat Recombinant Cell Line NF-κB Reporter (Luc) - A549 Recombinant Stable Cell Line NF-κB reporter (Luc) - HEK293 Recombinant Cell line NF-κB Reporter Kit (NF-κB Signaling Pathway) NFAT Reporter (Luc) Jurkat Recombinant Cell Line NFAT Reporter Hek293 Cell Line (PKC/ Ca2+ Pathway) Notch Signaling Pathway Notch1/CSL Reporter - HEK293 Recombinant Cell Line ONE-Step Luciferase Assay System - 10 ml ONE-Step Luciferase Assay System - 1L

6 SUPPLEMENTARY PRODUCTS One-Step luciferase detection system: Used to detect firefly luciferase expression following activation of luciferase reporter cell lines. Dual Luciferase Assay System: Used to monitor firefly (Phot inus pyralis) and Renilla luciferase activity in cultured mammalian cells. Ideal for highthroughput drug screening using the Firefly-Renilla dual luciferase reporter. QuickDetect Supplements: Used with IDO or TDO recombinant cell lines to activate IDO or TDO and detect conversion of tryptophan to kynurenine. OneStep Luciferase Assay System vs another commercially available luciferase reagent. JAK/STAT pathway ISRE Reporter? HEK293 Cells (BPSCat. # 60510) were seeded in a 96- well plate and treated with or without IFN to activate the JAK/STAT pathway. Next day, luciferase reagents were added to the cells and luminescence measured. Data shown as background subtracted luminescence. Media & Reagents Dual Luciferase (Firefly-Renilla) Assay System Dual Luciferase (Firefly-Renilla) Assay System Dual Luciferase (Firefly-Renilla) Assay System IDO1 Cellular Activity QuickDetect Supplements IDO1 Cellular Activity QuickDetect Supplements ONE-Step Luciferase Assay System - 10 ml ONE-Step Luciferase Assay System - 1L ONE-Step Luciferase Assay System - 10 ml ONE-Step Luciferase Assay System ml ONE-Step Luciferase Assay System ml TDO Cellular Activity QuickDetect Supplements TDO Cellular Activity QuickDetect Supplements Thaw Medium Thaw Medium Thaw Medium Thaw Medium Thaw Medium Thaw Medium Thaw Medium

7 SUPPLEMENTARY PRODUCTS Neutralizing Antibodies Block your cellular pathways by blocking protein interact ions in cellular assays. Recombinant monoclonal proteins. True clones, not contaminated by other antibodies. Greater specificity and consistency. Sequences are known and can be modified to include human Fc region (or other species). Highly suitable for specific cellular assays. Many are biotin-labeled with Avi-tag technology. Media & Reagents Dual Luciferase (Firefly-Renilla) Assay System Dual Luciferase (Firefly-Renilla) Assay System Dual Luciferase (Firefly-Renilla) Assay System IDO1 Cellular Activity QuickDetect Supplements IDO1 Cellular Activity QuickDetect Supplements ONE-Step Luciferase Assay System - 10 ml ONE-Step Luciferase Assay System - 1L ONE-Step Luciferase Assay System - 10 ml ONE-Step Luciferase Assay System ml ONE-Step Luciferase Assay System ml TDO Cellular Activity QuickDetect Supplements TDO Cellular Activity QuickDetect Supplements Thaw Medium Thaw Medium Thaw Medium Thaw Medium Thaw Medium Thaw Medium Thaw Medium

8 THE TRANSFECTION COLLECTION The Transfection Collection provides scientist a versatile and convenient package for their research needs. Bundle and save. Stable Cell Pack Contents: 1. Cell Line and/or cell line pairs 2. One Step detection reagent to detect luciferase activities (BPS #60690) 3. Specialized medium for growing and assaying the cells 4. Reporter vectors with firefly luciferase gene 6

9 THE TRANSFECTION COLLECTION The Transfection Collection Cell Packs bundle cell lines and components into single convenient packages for a one stop solution for: Monitoring signaling pathways via luciferase expression Transfecting cell lines for activator expression Measuring cellular binding and activation The Transfection Collection Transfection Collection : ISRE Transient Pack JAK/STAT Signaling Pathway Transfection Collection : AP1 Transient Pack (JNK Signaling Pathway) Transfection Collection : CRE/CREB Transient Pack (camp/pka Cell Signaling Pathway) Transfection Collection : GAL4 Transient Pack Glucocorticoid Receptor Pathway Transfection Collection : Myc Transient Pack (Myc Signaling Pathway) Transfection Collection : NF-κB Transient Pack ( NF-κB Signaling Pathway) Transfection Collection : NF-κB Reporter Cellular Assay Pack (CHOK1) Transfection Collection : NF-κB Reporter Cellular Assay Pack (HCT116) Transfection Collection : NF-κB Reporter Cellular Assay Pack (HEK293) Transfection Collection : Notch1/CSL Transient Pack (Notch Signaling Pathway) Transfection Collection : PDE4D Transient Pack Transfection Collection : RARα Reporter Cellular Assay Pack Transfection Collection : RARβ Reporter Cellular Assay Pack Transfection Collection : RARγ Reporter Cellular Assay Pack Transfection Collection : SBE Transient Pack (TGFβ/SMAD Signaling Pathway) Transfection Collection : SRE Transient Pack (MAPK/ERK Signaling Pathway) Transfection Collection : TCF/LEF Transient Pack Wnt / β-catenin Signaling Pathway

10 USES AND ADVANTAGES EXPRESSION CELL LINES ANTIBODY SCREENING FACS ANALYSIS BINDING STUDIES Example: OX40-HEK293 Recombinant Cell Line (Cat# 60682) SAMPLE DATA Flow cytometry analysis of cell surface expression of OX40 in OX40- HEK293 cells. OX40-HEK293 cells (green) or control HEK293 cells (blue) were stained with PE labeled Anti-OX40 Antibody (Cat# 72064) and analyzed by FACS. 8

11 USES AND ADVANTAGES CO-STIMULATORY CELL LINES INHIBITOR SCREENING ANTIBODY AFFINITY CO-CULTURE STUDIES EXAMPLES PD-1/ NFAT Reporter - Jurkat Recombinant Cell Line (Cat# 60535) PD-L1/ TCR Activator -CHO Recombinant Cell Line (Cat# 60536) SAMPLE DATA Anti-PD-L1 neutralizing antibody induced NFAT luciferase reporter activity in PD-1/NFAT Reporter- Jurkat cells co-cultured with HEK293 cells overexpressing PD-L1 and TCR activator. Anti-PD-1 neutralizing antibody induced NFAT luciferase reporter activity in PD-1/NFAT Reporter- Jurkat cells co-cultured with HEK293 cells overexpressing PD-L2 and TCR activator. 9

12 USES AND ADVANTAGES REPORTER CELL LINES MONITOR SIGNALING ACTIVITY MEASURE PROMOTER ACTIVITY MEASURE TRANSCRIPTION FACTOR ACTIVITY USE AS CONTROL CELL LINES EXAMPLE NFAT Reporter (Luc) - Jurkat Recombinant Cell LIne (Cat# 60621) SAMPLE DATA 10

13 CELL LINE LEASING OPTION: Try Before You Buy: Reliable assays Reproducible results Well validated and optimized We are confident that out cell lines will deliver and we want you to feel confident too! This program allows you a low-cost, low-risk option for validating the suitability of these unique cell lines in your own laboratories. Lease some of our most popular immunotherapy cell lines for up to three months. Following the rental period, the entire cost of leasing may be applied to the eventual purchase of the cells Contact a sales representative for details sales@bpsbioscience.com bpsbioscience.com/cell-line-rentals 11

14 BIOMOL GmbH Waidmannstr Hamburg Germany Fon: +49 (0) Fax: +49 (0)