Bringing Coatings to Life. It s Not Just a Good Slogan, It s A Great Business Plan. Bio-based Biocides. Fouling s New Arch Enemy

Transcription

1 Bringing Coatings to Life It s Not Just a Good Slogan, It s A Great Business Plan Bio-based Biocides Fouling s New Arch Enemy

2 Business Plan & Strategy for Product Innovation Integration of Two Industrial Sciences Biotechnology RSL Material Sciences DeFoul Anti-fouling marine coatings Caduceus Self-repairing materials Innovation ReNew Coatings re-uptaking releasable components FOR SALE FOR SALE (EPA) FOR SALE Anti-Microbial Enzymes Enzyme based antimicrobial materials OPDtox ProteCoat Coming Soon DeGreez Multi-Functional Materials Combinations of different additives Switchable Coatings Coatings whose RSL product activity is on/off induced 2

3 3 DeGreez

4 DeGreez Coating & Laminates that Self-Clean Common Oils Control DeGreez at work DeGreez Continuously hydrolyzes naturally-occurring oils upon contact Used in kitchen surfaces, air ducts, laminates, floor coatings Used in automobiles, touch screens 4

5 DeGreez in Action 5

6 NOW AVAILABLE erace Touch Screen Protectors with DeGreez Additives 6 6

7 7 OPDtox

8 OPDtox Detoxifies Nerve Agents and Pesticides Used for military vehicle coatings (tanks, aircraft, etc ) Used in textiles (uniforms, gear, etc of soldiers) Protects soldiers who may come in contact with contaminated surfaces Using actual weapons grade nerve agents, NATO has shown OPDtox coatings work. 8

9 OPDtox (WMDtox coatings) In Action 9

10 10 Protecoat

11 ProteCoat in Action 3-Year Exterior Exposure Testing Control Exterior Paint Developed for antimicrobial use Developed to be effective against bacteria, fungi & algae Designed for use in hospitals, industrial, architectural and hygienic coatings, automobiles, textiles, cements, mortars ProteCoat Exterior Paint 11

12 12 DeFoul

13 The Maritime Market Average Anti-Fouling surface area on ship approx 20,000 sq meters Requiring 1800 gals/ship Approx 16,000 ships at sea at any time If improve fuel consumption by 3 to 5 %, tremendous cost savings to the maritime industry Or, stated another way, increase of at least 2% in slip through the water viewed as a major improvement 13 Daily Shipping Activity Into North American Ports

14 One Month Static Shallow Submergence Results Based on ASTM D3623 Place of Immersion: Avery Point, Connecticut Depth of Immersion: 0.5 meters Length of Immersion: 4 weeks All results were the average of triplicate panels 14 14

15 One Month Static Shallow Submergence Results 15 15

16 One Month Static Shallow Submergence Results 16 16

17 One Month Static Shallow Submergence Results Industry-leading International Paint s Inter-Sleek 17 17

18 One Month Static Shallow Submergence Results 18 18

19 Tuning Anti-fouling Coatings Including Combinatorial Cocktails and Synergistic Killing 19 19

20 Building Combinatorial Libraries of Antimicrobial Peptides 6-mers were excellent anti-fungals but poor anti-bacterials. By adding only one AA, 7-mers also became excellent anti-bacterials 20

21 Synergism with Traditional Organic Biocides N2000, ppm AMP ProteCoat, ppm AMP-7, ppm 21

22 Synergism with Medical Antimicrobials NanoSilver Organic Biocide ProteCoat 22 22

23 Logarithmic Scale Quaternary Silane Nano Silver ProteCoat Synergism with Industry Leading Coating Biocides minute contact 8 hour contact 23

24 24 ReNew

25 Other Peptide Bioadditives Being Developed Metal (e.g., Copper) Binding Peptides for Use in Marine Coatings (i.e., DeFoul, ReNew) The Peptide may bind an Anti- Fouling Metal Either Released from the Marine Coating (e.g., a Metal Pigment) and /or Extract Dissolved Metal Ions from Sea Water

26 Natural Anti-fouling Functionalities 26

27 Coating with Metal Chelated Peptide A vinyl latex coating blended with a His6 metal binding peptide bound more free metal in solution than peptide free control coatings

28 Take Home Lessons to Date Bio-based additives work in anti-fouling coatings to reduce fouling in commercially significant amounts The anti-fouling effects of bio-based additives are likely caused by control of biofilm-generating microbes Industry-leading anti-fouling coatings can significantly benefit from adjuvant bio-based additives There are apparently different microbial targets for different bio-based additives Peptide additives that can be tuned to be more effective against specific microbes can likely be tuned to be more effective against marine microbes responsible for biofilm fouling 28 28

29 We Need To Do Next... Your help would be much appreciated... Including repeating and expanding static submersion testing in diverse sites and diverse seasons Including dynamic rotational (horizontal prop) submersion testing Industry input regarding augmenting existing antifouling coatings with bio-based adjuvant additives Including industry input regarding multi-cellular fouling organisms and relation to bio-film microorganisms... And... wee bits of funding to allow us to divert some resources to these studies 29

30 With Thanks to Our Collaborators: University of Southern Mississippi The Accelerator - USM Innovate Mississippi William-Carey University Mississippi Polymer Institute

31 31 Extra Reference Slides

32 Remember... virtually every manmade object in your personal space... Thus, just on a length by width basis, they represent a huge surface area surrounding you... But, the surface area they truly possess is anything BUT merely obtained using a calculation of their length and width... 32

33 33 1X view of a coating like on your computer or cell phone casing

34 34 An 800X close-up of that same coating

35 35 A 1600X cross-section of that same coating

36 Sizes of Bio-engineered Additives. Active Ingredient Enzyme Component of OPDtox,71.76 kilodaltons, 662 Amino acids Active Ingredient Peptide Component of ProteCoat, 959 daltons, 7 amino acids TiO 2 Pigment Typical bacterial cell, 2um x 0.45um, with a volume cubic micrometers PU film, pigment cubic micrometers, TiO cubic micrometers 36

37 ProteCoat Tested Against Traditional Biocides in Medical Coatings 37 37

38 NOW AVAILABLE erace Touch Screen Protectors with DeGreez Additives 38 38

39 Bio-based Coating Appliques Any Size, Any Shape, Any Surface 39

40 Reactive Surfaces Patent Thicket Foreign & US Patent Applications INNOVATION OPDtox DeGreez 2009 Caduceus 2010 Antimicrobial Enzymes 2008 DeFoul / ReNew 2010 Protecoat Switchable Coatings 40

41 OPDtox Antifouling Activity Prior Results About 3-5% (weight) OPDtox, a non-specific alkaline protease, or Protecoat were evaluated in a 2K emulsion blend water dispersed resin marine coating. The coatings were applied to steel surfaces, and cured hrs. Dry film thickness was 5-6 mils Coated steel plates were continuously submerged in Atlantic Ocean s waters for 1 month in the summer. All samples maintained the same adhesion and hardness properties relative to control samples lacking the bioadditives. No samples had corrosion. All samples were fouled. However, the fouling organisms/biofilms had reduced adherence on the OPDtox samples (e.g., mechanical scrapping was not needed to remove barnacles) relative to the other samples

42 Proposed Research Plan Combining Advanced Polymer Coatings and Reactive Surfaces Expertise Reactive Surfaces has sought possible 3 rd parties that could conduct foul release assays quickly and thoroughly to give options in conducting the assays needed to develop the coating. Based on our research, we recommend the following assays to evaluate foul release activity: a) A laboratory scale barnacle larvae (cyprid) adhesion assay. This would use a coated petri dish and Balanus amphitrite Darwin cyprids to evaluate the minimum additive needed to reduce attachment in a coating. Positive control coatings having known antifouling agents would be used for comparison. This is a 2 week assay

43 Proposed Research Plan Combining Advanced Polymer Coatings and Reactive Surfaces Expertise b) A dynamic seawater flow coated panel immersion assay. i. Coated panels for each formulation would be horizontally sliced though seawater at 15, 20 and 25 knots (at least 9 experimental coating panels and 9 additive free control panels). The water flow would be continuous for 11 hour intervals, plus 1 hour to do maintenance. The panels would be evaluated for barnacle adhesion at least one a month (biweekly if desired) for up to 4 months. ii. An alternating static vs. dynamic sea water flow coated panel assay. The panels would be 1 st immerged in seawater for a month (2 weeks minimum) to allow barnacle attachment, followed by a month of dynamic seawater exposure as described above. Another month of static sea water contact, followed by dynamic sea water contact, would provide a multi-cycle evaluation of foul release activity. A reduction of fouling after 5 hours of dynamic water contact at 20 knots is indicative of a commercially relevant product

44 Proposed Research Plan Combining Industry and Reactive Surfaces Expertise 44 c) A static seawater coated panel immersion assay. Coated panels for each formulation would be immersed in sea water to allow attachment of fouling organisms. At least 10 experimental coating panels and 10 additive free control panels would be evaluated for fouling on a monthly basis (biweekly if desired) for up to 4 months. d) An ecotoxicity assay of 3 coated panels to measure mortality over 24 to 48 hours for various marine organisms (e.g., fish, macroinvertebrates). Either static or flow through sea water may be used to elute any toxins from the coated panels. c) Upon achieving positive results, it is expected that continuous refinement and enhancements of activity would occur through multiple cycles of the testing described above. Additionally, other additives (e.g., Protecoat, and mixtures of enzyme(s) and peptide(s) would be evaluated to produce coatings with further enhanced foul release activity. 44