Plates available individually or as a set of 6. Each set contains 336 unique gene primer pairs deposited in one 96-well plate.

Transcription

1 ExProfile TM Human General Toxicology Related Gene qpcr Array For focused group profiling of human general toxicology genes expression Cat. No. QG020-A (4 x 96-well plate, Format A) Cat. No. QG020-B (4 x 96-well plate, Format B) Cat. No. QG020-C (4 x 96-well plate, Format C) Cat. No. QG020-D (4 x 96-well plate, Format D) Cat. No. QG020-E (4 x 96-well plate, Format E) Plates available individually or as a set of 6. Each set contains 336 unique gene primer pairs deposited in one 96-well plate. Introduction The ExProfile human general toxicology related gene qpcr array profiles the expression of 336 human genes related to the metabolic responses against drugs and other toxins. These genes are carefully chosen for their close pathway correlation based on a thorough literature search of peer-reviewed publications, mainly including genes involved in DNA damage and repair processes, apoptosis, cell cycle, electron transport, growth and proliferation, metabolite transporters, as well as stress response genes. This array allows researchers to study the pathway-related genes to gain understanding of their roles in the metabolic responses against drugs and other toxins. QG020 plate 01: 84 unique gene PCR primer pairs QG020 plate 02: 84 unique gene PCR primer pairs QG020 plate 03: 84 unique gene PCR primer pairs QG020 plate 04: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A (96-well) Applied Biosystems 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) B (96-well) Applied Biosystems 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7 (Fast block) C (96-well) Bio-Rad Laboratories icycler iq,myiq,iq 5 1

2 D (96-well) Bio-Rad Laboratories CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 E (96-well) Roche Applied Science LightCycler 480 (96-well block) Quality control 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG020 plate 01 Figure2. Illustration of QG020 plate 02 2

3 Figure3. Illustration of QG020 plate 03 Figure4. Illustration of QG020 plate 04 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. Gene primer list Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ TNFRSF1A QG A02 HQP NM_ TNFRSF11B QG A03 HQP NM_ TNFRSF11A QG A04 HQP NM_ TNFRSF10A QG A05 HQP NM_ TNFAIP3 QG A06 HQP NM_ TNF QG A07 HQP NM_ TH QG A08 HQP NM_ TGFBR1 QG A09 HQP NM_ TGFB2 QG A10 HQP NM_ TGFB1 3

4 4 QG A11 HQP NM_ TGFA QG A12 HQP NM_ TFDP1 QG B01 HQP NM_ TDP1 QG B02 HQP NM_ TDG QG B03 HQP NM_ TCP1 QG B04 HQP NM_ TAP1 QG B05 HQP NM_ SULT1E1 QG B06 HQP NM_ SULT1A1 QG B07 HQP NM_ STAT5A QG B08 HQP NM_ SRD5A2 QG B09 HQP NM_ SOD2 QG B10 HQP NM_ SOD1 QG B11 HQP NM_ SLCO1C1 QG B12 HQP NM_ SLC2A1 QG C01 HQP NM_ SLC27A4 QG C02 HQP NM_ SLC27A1 QG C03 HQP NM_ SLC10A2 QG C04 HQP NM_ SLC10A1 QG C05 HQP NM_ SIRT1 QG C06 HQP NM_ SHH QG C07 HQP NM_ SERPINE1 QG C08 HQP NM_ SDHB QG C09 HQP NM_ SCP2 QG C10 HQP NM_ SCD QG C11 HQP NM_ SAA1 QG C12 HQP NM_ RXRB QG D01 HQP NM_ RXRA QG D02 HQP NM_ RELB QG D03 HQP NM_ RB1 QG D04 HQP NM_ RARA QG D05 HQP NM_ RAG1 QG D06 HQP NM_ RAF1 QG D07 HQP NM_ RAD52 QG D08 HQP NM_ RAD51 QG D09 HQP NM_ RAD50 QG D10 HQP NM_ RAD23A QG D11 HQP NM_ RAD21 QG D12 HQP NM_ RAD18 QG E01 HQP NM_ RAD1 QG E02 HQP NM_ RAC1 QG E03 HQP NM_ PTGS2 QG E04 HQP NM_ PTGS1 QG E05 HQP NM_ PTEN QG E06 HQP NM_ PTCH1 QG E07 HQP NM_ PRDX1 QG E08 HQP NM_ PPARGC1A

5 5 QG E09 HQP NM_ PPARG QG E10 HQP NM_ PPARD QG E11 HQP NM_ POR QG E12 HQP NM_ PON3 QG F01 HQP NM_ POLL QG F02 HQP NM_ POLK QG F03 HQP NM_ POLI QG F04 HQP NM_ POLB QG F05 HQP NM_ PMS2 QG F06 HQP NM_ PLTP QG F07 HQP NM_ PLA2G2A QG F08 HQP NM_ PKLR QG F09 HQP NM_ PGR QG F10 HQP NM_ PERP QG F11 HQP NM_ PCNA QG F12 HQP NM_ ORM1 QG G01 HQP NM_ NRAS QG G02 HQP NM_ NR1I2 QG G03 HQP NM_ NQO1 QG G04 HQP NM_ NOS3 QG G05 HQP NM_ NOS2A QG G06 HQP NM_ NNMT QG G07 HQP NM_ NFKB1 QG G08 HQP NM_ NF1 QG G09 HQP NM_ NDUFV1 QG G10 HQP NM_ NDUFS4 QG G11 HQP NM_ NDUFS1 QG G12 HQP NM_ NAT2 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR QG A01 HQP NM_ NAT1 QG A02 HQP NM_ MYC QG A03 HQP NM_ MVP QG A04 HQP NM_ MTTP QG A05 HQP NM_ MT3 QG A06 HQP NM_ MT2A

6 6 QG A07 HQP NM_ MSH6 QG A08 HQP NM_ MSH2 QG A09 HQP NM_ MPO QG A10 HQP NM_ MPG QG A11 HQP NM_ MMP9 QG A12 HQP NM_ MMP2 QG B01 HQP NM_ MLL QG B02 HQP NM_ MLH1 QG B03 HQP NM_ MIF QG B04 HQP NM_ MGST1 QG B05 HQP NM_ MET QG B06 HQP NM_ MDM2 QG B07 HQP NM_ MCL1 QG B08 HQP NM_ MBL2 QG B09 HQP NM_ MBD4 QG B10 HQP NM_ MB QG B11 HQP NM_ MAPK8 QG B12 HQP NM_ MAPK1 QG C01 HQP NM_ MAOB QG C02 HQP NM_ MAOA QG C03 HQP NM_ LTBR QG C04 HQP NM_ LTA QG C05 HQP NM_ LIG4 QG C06 HQP NM_ LIG1 QG C07 HQP NM_ LDLR QG C08 HQP NM_ LBP QG C09 HQP NM_ JUNB QG C10 HQP NM_ JUN QG C11 HQP NM_ IVD QG C12 HQP NM_ ITGB2 QG D01 HQP NM_ ITGAL QG D02 HQP NM_ INS QG D03 HQP NM_ IL6 QG D04 HQP NM_ IL1B QG D05 HQP NM_ IL1A QG D06 HQP NM_ IL18 QG D07 HQP NM_ IGFBP6 QG D08 HQP NM_ IGFBP3 QG D09 HQP NM_ IGFBP2 QG D10 HQP NM_ IGFBP1 QG D11 HQP NM_ IGF2R QG D12 HQP NM_ IGF1R QG E01 HQP NM_ IGF1 QG E02 HQP NM_ HYOU1 QG E03 HQP NM_ HUS1 QG E04 HQP NM_ HSPD1

7 7 QG E05 HQP NM_ HSPB2 QG E06 HQP NM_ HSPB1 QG E07 HQP NM_ HSPA9 QG E08 HQP NM_ HSPA5 QG E09 HQP NM_ HSPA2 QG E10 HQP NM_ HSPA1L QG E11 HQP NM_ HSPA1B QG E12 HQP NM_ HSPA1A QG F01 HQP NM_ HSP90AA1 QG F02 HQP NM_ HSF1 QG F03 HQP NM_ HPX QG F04 HQP NM_ HPRT1 QG F05 HQP NM_ HMOX1 QG F06 HQP NM_ HIF1A QG F07 HQP NM_ HGF QG F08 HQP NM_ HBEGF QG F09 HQP NM_ HAO1 QG F10 HQP NM_ GTF2H1 QG F11 HQP NM_ GSTA4 QG F12 HQP NM_ GSR QG G01 HQP NM_ GSK3B QG G02 HQP NM_ GPX5 QG G03 HQP NM_ GPX2 QG G04 HQP NM_ GPX1 QG G05 HQP NM_ GPD2 QG G06 HQP NM_ GAP43 QG G07 HQP NM_ GADD45A QG G08 HQP NM_ FXYD2 QG G09 HQP NM_ FOXO3A QG G10 HQP NM_ FOXM1 QG G11 HQP NM_ FOS QG G12 HQP NM_ FMO4 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR QG A01 HQP NM_ FMO3 QG A02 HQP NM_ FMO1

8 8 QG A03 HQP NM_ FGFR2 QG A04 HQP NM_ FGF2 QG A05 HQP NM_ FGF1 QG A06 HQP NM_ FEN1 QG A07 HQP NM_ FDXR QG A08 HQP NM_ FASLG QG A09 HQP NM_ FAS QG A10 HQP NM_ FANCC QG A11 HQP NM_ FANCA QG A12 HQP NM_ FADD QG B01 HQP NM_ FABP7 QG B02 HQP NM_ FABP1 QG B03 HQP NM_ ESR2 QG B04 HQP NM_ ESR1 QG B05 HQP NM_ ERCC3 QG B06 HQP NM_ ERCC2 QG B07 HQP NM_ ERCC1 QG B08 HQP NM_ ERBB3 QG B09 HQP NM_ ERBB2 QG B10 HQP NM_ EPX QG B11 HQP NM_ EPHX1 QG B12 HQP NM_ ELK1 QG C01 HQP NM_ EGR1 QG C02 HQP NM_ EGFR QG C03 HQP NM_ EGF QG C04 HQP NM_ EDNRB QG C05 HQP NM_ EDN1 QG C06 HQP NM_ E2F4 QG C07 HQP NM_ E2F1 QG C08 HQP NM_ DPYD QG C09 HQP NM_ DNAJB1 QG C10 HQP NM_ DNAJA1 QG C11 HQP NM_ DIABLO QG C12 HQP NM_ DHFR QG D01 HQP NM_ DDIT3 QG D02 HQP NM_ DDC QG D03 HQP NM_ DDB2 QG D04 HQP NM_ DCLRE1C QG D05 HQP NM_ DAP QG D06 HQP NM_ CYP8B1 QG D07 HQP NM_ CYP7B1 QG D08 HQP NM_ CYP7A1 QG D09 HQP NM_ CYP51A1 QG D10 HQP NM_ CYP4B1 QG D11 HQP NM_ CYP4A11 QG D12 HQP NM_ CYP46A1

9 9 QG E01 HQP NM_ CYP3A5 QG E02 HQP NM_ CYP3A4 QG E03 HQP NM_ CYP2S1 QG E04 HQP NM_ CYP2F1 QG E05 HQP NM_ CYP2E1 QG E06 HQP NM_ CYP2D6 QG E07 HQP NM_ CYP2C9 QG E08 HQP NM_ CYP2B6 QG E09 HQP NM_ CYP2A13 QG E10 HQP NM_ CYP27B1 QG E11 HQP NM_ CYP27A1 QG E12 HQP NM_ CYP26A1 QG F01 HQP NM_ CYP24A1 QG F02 HQP NM_ CYP1B1 QG F03 HQP NM_ CYP1A2 QG F04 HQP NM_ CYP1A1 QG F05 HQP NM_ CYP19A1 QG F06 HQP NM_ CYP17A1 QG F07 HQP NM_ CYP11A1 QG F08 HQP NM_ CYB5R3 QG F09 HQP NM_ CXCL10 QG F10 HQP NM_ CTPS QG F11 HQP NM_ CTNNB1 QG F12 HQP NM_ CSK QG G01 HQP NM_ CSF2 QG G02 HQP NM_ CPT1A QG G03 HQP NM_ CP QG G04 HQP NM_ COMT QG G05 HQP NM_ COL1A1 QG G06 HQP NM_ CLU QG G07 HQP NM_ CHEK2 QG G08 HQP NM_ CHAT QG G09 HQP NM_ CES1 QG G10 HQP NM_ CDKN2A QG G11 HQP NM_ CDKN1B QG G12 HQP NM_ CDKN1A QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT

10 10 QG H11 PCR QG H12 PCR QG A01 HQP NM_ CDK4 QG A02 HQP NM_ CDK2 QG A03 HQP NM_ CDC6 QG A04 HQP NM_ CDC37 QG A05 HQP NM_ CDC25C QG A06 HQP NM_ CDC25B QG A07 HQP NM_ CDC25A QG A08 HQP NM_ CDC20 QG A09 HQP NM_ CD40 QG A10 HQP NM_ CD36 QG A11 HQP NM_ CCNG2 QG A12 HQP NM_ CCNE1 QG B01 HQP NM_ CCND2 QG B02 HQP NM_ CCND1 QG B03 HQP NM_ CCNC QG B04 HQP NM_ CCNB1 QG B05 HQP NM_ CCNA2 QG B06 HQP NM_ CCL4 QG B07 HQP NM_ CASP9 QG B08 HQP NM_ CASP8 QG B09 HQP NM_ CASP3 QG B10 HQP NM_ BRCA2 QG B11 HQP NM_ BRCA1 QG B12 HQP NM_ BOK QG C01 HQP NM_ BNIP3L QG C02 HQP NM_ BNIP3 QG C03 HQP NM_ BLM QG C04 HQP NM_ BIRC5 QG C05 HQP NM_ BID QG C06 HQP NM_ BCR QG C07 HQP NM_ BCL6 QG C08 HQP NM_ BCL2L1 QG C09 HQP NM_ BCL2 QG C10 HQP NM_ BCHE QG C11 HQP NM_ BAX QG C12 HQP NM_ BAK1 QG D01 HQP NM_ BAG1 QG D02 HQP NM_ BAD QG D03 HQP NM_ ATP2A1 QG D04 HQP NM_ ATM QG D05 HQP NM_ ARNT QG D06 HQP NM_ AR QG D07 HQP NM_ APP QG D08 HQP NM_ APOE

11 11 QG D09 HQP NM_ APOC2 QG D10 HQP NM_ APOA1 QG D11 HQP NM_ APEX1 QG D12 HQP NM_ APC QG E01 HQP NM_ APAF1 QG E02 HQP NM_ AOC3 QG E03 HQP NM_ ANXA5 QG E04 HQP NM_ ALOX5 QG E05 HQP NM_ ALDH1A1 QG E06 HQP NM_ AKT1 QG E07 HQP NM_ AHR QG E08 HQP NM_ ADFP QG E09 HQP NM_ ACAT1 QG E10 HQP NM_ ABP1 QG E11 HQP NM_ ABL1 QG E12 HQP NM_ ABCG8 QG F01 HQP NM_ ABCG5 QG F02 HQP NM_ ABCG2 QG F03 HQP NM_ ABCD3 QG F04 HQP NM_ ABCD2 QG F05 HQP NM_ ABCD1 QG F06 HQP NM_ ABCC6 QG F07 HQP NM_ ABCC5 QG F08 HQP NM_ ABCC3 QG F09 HQP NM_ ABCC2 QG F10 HQP NM_ ABCC1 QG F11 HQP NM_ ABCB4 QG F12 HQP NM_ ABCB11 QG G01 HQP NM_ ABCB1 QG G02 HQP NM_ ABCA1 QG G03 HQP NM_ GAB1 QG G04 HQP NM_ GADD45B QG G05 HQP NM_ GCDH QG G06 HQP NM_ NDUFB7 QG G07 HQP NM_ NTHL1 QG G08 HQP NM_ PKMYT1 QG G09 HQP NM_ PMAIP1 QG G10 HQP NM_ APRT QG G11 HQP NM_ ABCG1 QG G12 HQP NM_ ACADSB QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A

12 QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 12

13 Limited Use License Following terms and conditions apply to use of ExProfile TM Human General Toxicology Related Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX,ViiA TM,StepOnePlus TM (Life Technologies). QG