Avidin/Biotin Blocking Kit

Transcription

1 Version 1 Last updated 25 June 2018 ab64212 Endogenous Avidin/Biotin Blocking Kit For the blocking of endogenous biotin during immunohistochemistry. This product is for research use only and is not intended for diagnostic use. ab64212 Endogenous Avidin/Biotin Blocking Kit 1

2 Table of Contents 1. Overview 1 2. Quick Assay Procedure 2 3. Materials Supplied and Storage 3 4. Materials Required, Not Supplied 4 5. General guidelines, precautions, and troubleshooting 5 6. Reagent Preparation 6 7. Standard Preparation 7 8. Sample Preparation 8 9. Assay Procedure Data Analysis FAQs / Troubleshooting Typical Data Notes 13 ab64212 Endogenous Avidin/Biotin Blocking Kit 2

3 1. Overview In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens. Incubate tissue sections with avidin block for minutes Wash 2 times with buffer Incubate tissue sections with biotin block for minutes Wash 2 times with buffer Apply primary antibody to the tissue sections ab64212 Endogenous Avidin/Biotin Blocking Kit 3

4 2. Materials Supplied and Storage Store kit at 4 C immediately on receipt and check below for storage for individual components. Kit can be stored for 1 year from receipt, if components have not been reconstituted. Aliquot components in working volumes before storing at the recommended temperature. Avoid repeated freeze-thaws of reagents. Item Quantity Storage temperatur e (before prep) Storage temperatur e (after prep) Avidin Block 15 ml 4 C N/A Biotin Block 15 ml 4 C N/A ab64212 Endogenous Avidin/Biotin Blocking Kit 4

5 3. Materials Required, Not Supplied These materials are not included in the kit, but will be required to successfully perform this assay: Buffer of choice for washing tissue sections ab64212 Endogenous Avidin/Biotin Blocking Kit 5

6 4. General guidelines, precautions, and troubleshooting Please observe safe laboratory practice and consult the safety datasheet. For general guidelines, precautions, limitations on the use of our assay kits and general assay troubleshooting tips, particularly for first time users, please consult our guide: For typical data produced using the assay, please see the assay kit datasheet on our website. ab64212 Endogenous Avidin/Biotin Blocking Kit 6

7 5. Reagent Preparation 5.1 Avidin Block Ready to use as supplied. 5.2 Biotin Block Ready to use as supplied. ab64212 Endogenous Avidin/Biotin Blocking Kit 7

8 6. Assay Procedure 6.1 Blocking/staining protocol: 1. Incubate tissue sections with avidin block for minutes. 2. Wash 2 times with buffer. 3. Incubate tissue sections with biotin block for minutes. 4. Wash 2 times with buffer. 5. Apply primary antibody to the tissue sections. ab64212 Endogenous Avidin/Biotin Blocking Kit 8

9 7. FAQs / Troubleshooting General troubleshooting points are found at ab64212 Endogenous Avidin/Biotin Blocking Kit 9

10 ab64212 Endogenous Avidin/Biotin Blocking Kit 10

11 8. Notes ab64212 Endogenous Avidin/Biotin Blocking Kit 11

12 Technical Support Copyright Abcam. All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. Austria wissenschaftlicherdienst@abcam.com France supportscientifique@abcam.com Germany wissenschaftlicherdienst@abcam.com Spain soportecientifico@abcam.com Switzerland technical@abcam.com Deutsch: Français: UK, EU and ROW technical@abcam.com +44(0) Canada ca.technical@abcam.com US and Latin America us.technical@abcam.com Asia Pacific hk.technical@abcam.com (852) China cn.technical@abcam.com Japan technical@abcam.co.jp +81-(0) Singapore sg.technical@abcam.com Australia au.technical@abcam.com +61-(0) New Zealand nz.technical@abc.com +64-(0) Copyright 2018 Abcam. All rights reserved