XXI INTERNATIONAL SCHOOL OF PURE AND APPLIED BIOPHYSICS. Time Resolved Methods in Biophysics. Palazzo Franchetti January 9-13, 2017, Venice, Italy

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1 XXI INTERNATIONAL SCHOOL OF PURE AND APPLIED BIOPHYSICS Time Resolved Methods in Biophysics Palazzo Franchetti January 9-13, 2017, Venice, Italy Organised by: SIBPA - Società Italiana di Biofisica Pura e Applicata IVSLA - Istituto Veneto di Scienze Lettere ed Arti Director of the School Giorgio M. Giacometti (IVSLA and University of Padova) Scientific Coordinators Cristiano Viappiani (Università degli Studi di Parma) Thomas Gensch (Forschungzentrum Jülich, Germany) Giorgio M. Giacometti (IVSLA and University of Padova) The School is held in the magnificent Palazzo Franchetti, the premises of the "Istituto di Scienze Lettere ed Arti" located in the historical centre of Venice, close to Canal Grande and Accademia (Palazzo Cavalli-Franchetti).

2 The focus of the 2017 School is Time Resolved Methods in Biophysics. The possibility of accessing structural, topological or spectroscopic information with the proper time resolution is at the basis of a variety of methods that are currently applied in biophysics. These methods allow unprecedented understanding of many biologically relevant processes such as mechanistics of biochemical reactions, energy transfer and fixation, protein folding, macromolecular conformational changes and cellular trafficking, to name just a few. This school proposes an overview on a selection of current spectroscopic, structural and microscopy methods that are inherently endowed with time resolution. This year the School is organized with the contribution of: The school has the patronage of the University of Parma

3 Program of the school Monday, January STED/RESOLFT optical nanoscopy for the life sciences Ilaria Testa, KTH Royal Institute of Technology, Stockholm, Sweden Infrared Spectroscopy on Biomolecules Welcome drink Tuesday, January 10 Tilman Kottke, University Bielefeld, Germany Applications of Time-resolved FTIR Spectroscopy Tilman Kottke, University Bielefeld, Germany Femtosecond infrared and visible spectroscopy; instrumentation, analysis and theory Jasper van Thor, Imperial College London, UK Time and Space in Super Resolved Microscopy Alberto Diaspro, Istituto Italiano di Tecnologia, Genova, Italy Ultrafast structural dynamics in protein crystals Jasper van Thor, Imperial College London, UK Resources at the Nikon Imaging Center and Eurobioimaging Alberto Diaspro, Istituto Italiano di Tecnologia, Genova, Italy Time-resolved X-ray scattering: a tool to investigate the structural dynamics of proteins in solution. Part Coffee break Matteo Levantino, ESRF, Grenoble, France Time-resolved X-ray scattering: a tool to investigate the structural dynamics of proteins in solution. Part 2. Matteo Levantino, ESRF, Grenoble, France

4 Wednesday, January Coherent Raman Microscopy Dario Polli, Politecnico di Milano, Italy Laser based triggering methods Coffee break Cristiano Viappiani, Università di Parma, Italy Theory and applications of flash photolysis in Biophysics Maria Tesa, Edinburgh Instruments, UK Microscopy Camera Technologies: Understanding CCDs and scmos cameras Hamamatsu Giulio Simonutti Genetically encoded fluorescent biosensors Thomas Gensch, Forschungszentrum Jülich, Germany Time-correlated single photon counting in fluorescence Wolfgang Becker, Becker&Hickl, Germany Single molecule Biophysics: Basics Coffee break Johan Hofkens, KU Leuven, Belgium Single molecule Biophysics: Applications Thursday, January 12 Johan Hofkens, KU Leuven, Belgium Single-molecule super-resolution microscopy in cells Mike Heilemann, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany Lifetime-based fluorescence sensing Ranieri Bizzarri, CNR-Istituto Nanoscienze, Pisa, Italy

5 Determination of [Cl - ] int in acute brain slices using FLIM Verena Untiet, :Forschungszentrum Jülich, Germany Overview of cryo-em and examples of snapshots over time Friday, January 13 Matteo Allegretti, European Molecular Biology Laboratory, Heidelberg, Germany Free afternoon Time-resolved studies of protein structures, the biochemist's point of view Beatrice Vallone, Università di Roma La Sapienza, Roma, Italy Time-resolved serial femtosecond crystallography Martin Weik, Institut de Biologie Structurale, Grenoble, France RapidFLIM - the new and innovative method for ultra-fast FLIM imaging Uwe Ortmann, Picoquant, Berlin, Germany Time-resolved absorption and emission techniques for the study of photosensitisation processes Santi Nonell, Institut Quimic de Sarria, Barcelona, Spain Spatiotemporal fluctuation analysis: a powerful tool for the future nanoscopy of molecular processes. Part 1 Francesco Cardarelli, Istituto Italiano di Tecnologia, Pisa, Italy Spatiotemporal fluctuation analysis: a powerful tool for the future nanoscopy of molecular processes. Part Final remarks Francesco Cardarelli, Istituto Italiano di Tecnologia, Pisa, Italy Further information is available through the scientific coordinators Cristiano Viappiani cristiano.viappiani@unipr.it Thomas Gensch t.gensch@fz-juelich.de