CHARACTERIZATION & PURIFICATION

Transcription

1 Ultra-High Efficiency GFC/SEC BIOMOLECULE CHARACTERIZATION & PURIFICATION AT EXTREMELY AFFORDABLE PRICES AGGREGATES ADC mab BIOSIMILARS NEW 1.8 µm SEC-X1 Replace Waters BEH 1.7 µm SEC columns to: Save Money Minimize Adsorption Maximize Separation Range

2 Welcome to Affordable, Higher Resolution Size Exclusion Chromatography With Yarra SEC Columns you can: Save Money with extremely affordable prices Achieve Better Results through larger exclusion ranges and higher efficiencies Get Enhanced Recovery using more inert Yarra particles and Bio-Inert hardware Gain Time with faster more productive HPLC/UHPLC runs on the new 1.8 µm SEC-X1 Feel at Ease knowing you have an unmatched product guarantee 1 Phenomenex, Inc. All rights reserved.

3 Ultra-High Efficiency GFC/SEC Better Protein/Peptide SEC Starts With Yarra Affordable Pricing and Unmatched Product Guarantee... Dependable Results... NEW 1.8 µm with Bio-Inert Hardware 3 µm Exceptional Separation Power (Insulin / mabs) Expanded Exclusion Range... 8 Better Recovery with Greater Inertness... 9 HPLC/UHPLC System Versatility... 1 Incredible Performance Upgrade Excellent Lifetime and Stability... 1 Simple Upgrade for µm SEC Methods Excellent Lifetime and Stability... 1 µm High Performance Preparative SEC Select the Right Column for Your Application...1 Ordering Information...1 3

4 Affordable Pricing and Unmatched Product Guarantee Yarra SEC columns are highly affordable and are accompanied by a product guarantee that ensures performance satisfaction, making it extremely easy to upgrade current SEC methods! If Yarra analytical columns do not provide you with at least an equivalent separation as any other GFC column of similar porosity, type, and dimensions, return the column with comparative data within days for a FULL REFUND. Yarra vs. Waters BEH SEC Yarra 1.8 µm 1 x. mm SEC-X K-K >3, vs. Particle Size (µm) Pore Size (Å) MW Range in Native Conditions (Da) Efficiency (plates/column) Waters* 1.7 µm 1 x. mm BEH1 SEC BEH SEC K 8K 1K K >3, >3, *Waters specifications taken from Waters website Yarra vs. Tosoh Bioscience TSKgel Yarra 3 x 7.8 mm TSKgel 3 x 7.8 mm SEC- SEC-3 SEC- vs. GSWxl G3SWxl GSWxl K 3K K 7K 1K 1,K 8, 8, 38, Particle Size (µm) Pore Size (Å) MW Range in Native Conditions (Da) Efficiency (minimum theoretical plates) 8 1 K 1K 1K K K 7,K,, 1, All TSKgel specifications were taken from Tosoh Bioscience - Laboratory Products Catalog Comparative separations may not be representative of all applications. Phenomenex l WEB:

5 Expert Manufacturing for Dependable Performance and Reliability Ultra-High Efficiency GFC/SEC Reproducible GFC columns require extreme detail in every aspect of the manufacturing and packing process. First, Yarra silica particles are synthesized using narrow tolerances for pore and particle size. Next, bonding of a proprietary hydrophilic ligand is tightly controlled and packing of each column requires validated recipes tested to high specifications to ensure excellent reproducibility. Batch-to-Batch Reproducibility Yarra 1.8 µm SEC-X1 3 1 mau App ID 33 Column: Yarra 1.8 µm SEC-X1 Dimensions: 1 x. mm Part No.: F-31-E Mobile Phase: 1 mm Sodium Phosphate in Water ph.8 +. % NaN 3 Flow Rate:.3 ml/min Detection: 8 nm Sample: 1. Thyroglobulin (9 kda). IgA (3 kda) 3. IgG (1 kda). Ovalbumin ( kda). Myoglobin (17 kda). Uridine min Batch-to-Batch Reproducibility Yarra 3 µm SEC-3 mau Column: Yarra 3 µm SEC-3 Dimensions: 3 x 7.8 mm Part No.: H-13-K Mobile Phase: 1 mm Sodium Phosphate in Water ph.8 +. % NaN 3 Flow Rate: 1 ml/min Detection: 8 nm Sample: 1. Thyroglobulin (9 kda). IgA (3 kda) 3. IgG (1 kda). Ovalbumin ( kda). Myoglobin (17 kda). Uridine 1 1 App ID min

6 Exceptional Separation Power Low MW Degraded Human Insulin AU Yarra 1.8 µm SEC-X1 AU Waters BEH1 SEC 1.7 µm....1 Greater separation of dimer and high molecular weight aggregate App ID 318. App ID min min Human Insulin Yarra 1.8 µm SEC-X1 Waters BEH1 SEC 1.7 µm Greater Resolution! App ID 319 App ID 31 min Conditions for all columns: Column: Yarra 1.8 µm SEC-X1 BEH1 SEC, 1.7 µm Dimensions: 1 x. mm Mobile Phase: 1 g/l L-Arginine in Water/Acetic Acid/Acetonitrile (:1:) Flow Rate:. ml/min Detection: 8 nm Sample: Human Insulin min Comparative separations may not be representative of all applications.

7 Exceptional Separation Power High MW Ultra-High Efficiency GFC/SEC The greater resolving power provided by the Yarra 1.8 µm SEC-X1 means you will have better separation between the proteins that are of the most interest to you. In this example you can easily see separation of antibody aggregates from the Ab monomer. mau 1 Column: Dimensions: Part No.: Mobile Phase: Yarra 1.8 µm SEC-X1 1 x. mm F-31-E 1 mm Sodium Phosphate in Water ph.8 Flow Rate:.3 ml/min Detection: 8 nm Sample: Mouse Monoclonal Ab Yarra 1.8 μm SEC-X1 provides resolution of multiple aggregate species 8 App ID Phenomenex l 7 min WEB: 7

8 Expanded Separation Window An expanded exclusion range was expertly developed and fine-tuned for the Yarra 1.8 µm, resulting in a wider applicability for popular biomolecule separations. This inherently saves both time and money for scientists by negating the need to spend money on two or more expensive phases.* 1,, Yarra 1.8 µm SEC-X1, 1 x. mm Protein MW (Da) 1, 1, Waters 1.7 µm BEH, 1 x. mm Waters 1.7 µm BEH1, 1 x. mm 1, Relative Elution Volume (ml/cv) Substantial Savings with a Single Column Selection Representative pricing found in USA market. Low MW Biomolecules High MW Biomolecules List Price (1 x. mm) Waters BEH1 SEC 1.7 µm $,3 Waters BEH SEC 1.7 µm $,3 Price to do Low and High MW $,7 Yarra 1.8 µm SEC-X1 $1,9 $1,9 $,777 Savings with Yarra 1.8 µm SEC-X1 Column Waters pricing taken from Waters website on 8//1 at 3:3pm *Comparative separations may not be representative of all applications. 8 Phenomenex l WEB:

9 Better Recovery with Greater Inertness Ultra-High Efficiency GFC/SEC Phenomenex s proprietary surface chemistry provides an inertness that is hard to match by other GFC columns. The result is minimal adsorption of peptides, proteins, and other protein aggregates leading to more accurate quantitation. AU.3.8 Yarra 1.8 µm SEC-X1 Waters BEH SEC 1.7 µm Injection 9 Injection 1 AU..1. Greater. Inertness Means No Priming Necessary! Injection Injection App ID App ID min min Conditions for all columns: Columns: Yarra 1.8 µm SEC-X1 BEH SEC 1.7 µm Dimensions: 1 x. mm Mobile Phase: mm Sodium Phosphate ph./.1 M Sodium Sulfate Flow Rate:.3 ml/min Temperature: Ambient Detection: 8 nm Sample: 1. IgG Dimer. IgG Monomer 3. Albumin New Bio-Inert Hardware In addition to a highly inert particle chemistry, Yarra 1.8 µm SEC-X1 columns utilize a Bio-Inert hardware to further ensure accurate recoveries. Comparative separations may not be representative of all applications. 9

10 Fast GFC/SEC methods on your HPLC or UHPLC Utilize the high performance of the new Yarra 1.8 µm SEC-X1 on the system(s) that you know and are comfortable with! The amazing separation power of the Yarra 1.8 µm SEC-X1 can easily be utilized on any HPLC and UHPLC system. Waters ACQUITY UPLC Shimadzu Nexera Agilent 1 Questions About LC System Compatibility? Contact your local Phenomenex representative for guidance! 1

11 Performance Upgrade Ultra-High Efficiency GFC/SEC Upgrading to better performance from existing SEC columns is easy with the Yarra column line. Yarra 3 µm particle columns are a quick replacement for existing µm columns, giving increased performance and better peak shape without method adjustment. However, large gains in analyses times, resolution and efficiency can be realized with the Yarra 1.8 µm SEC-X1 on the desired HPLC/UHPLC system. Conventional µm 3 x 7.8 mm 1 3 App ID min Yarra 3 μm SEC- 3 x 7.8 mm 3 1 App ID min Yarra 1.8 μm SEC-X1 1 x. mm 3 1 Higher efficiency and much faster analysis App ID min Conditions for all columns: Temperature: Ambient Columns: Conventional µm Yarra 3 µm SEC- Yarra 1.8 µm SEC-X1 Dimensions: As noted in chromatograms Mobile Phase: 1 mm Sodium Phosphate in Water ph.8 +. % NaN 3 Flow Rate: 1 ml/min.3 ml/min (Yarra 1.8 µm SEC-X1 1 x. mm) Comparative separations may not be representative of all applications. Detection: 8 nm Sample: 1. Thyroglobulin (9 kda). IgA (3 kda) 3. IgG (1 kda). Ovalbumin ( kda). Myoglobin (17 kda). Uridine 11

12 Excellent Stability and Lifetime The great care exhibited in both engineering and packing of Yarra columns can produce stable performance over longer lifetimes than other size exclusion columns on the market. By gaining extended lifetime with Yarra columns, you save significant time and money for your lab. Increased Lifetime Under Extreme Conditions (1 % IPA and higher flow rate) mau 3 3 No Change in: Peak shape Efficiency Backpressure 1 1 Time = Hour 1 Time = Hour 1 App ID min Column: Yarra 1.8 µm SEC-X1 Dimension: 1 x. mm Part No.: F-31-E Mobile Phase: 1 mm Sodium Phosphate in Water ph.8 +. % NaN % IPA Flow Rate:. ml/min Temperature: Ambient Detection: 8 nm Sample: 1. Thyroglobulin (9 kda). IgA (3 kda) 3. IgG (1 kda). Ovalbumin ( kda). Myoglobin (17 kda). Uridine Exceptional Stability Over 9 Injections Minimal change in retention and performance Number of Plates per Column Injections Retention time App ID Retention Time (min) Column: Yarra 3 µm SEC- Dimensions: 3 x 7.8 mm Part No.: H-1-K Guard Cartridge: AJ-87 Guard Holder: KJ-8, SecurityGuard Guard Cartridge Kit Mobile Phase: 1 mm Sodium Phosphate in Water ph.8 +. % NaN 3 Flow Rate: 1 ml/min Detection: 8 nm Sample: 1. Thyroglobulin (9 kda). IgA (3 kda) 3. IgG (1 kda). Ovalbumin ( kda). Myoglobin (17 kda). Uridine Plates/column 1

13 High Performance Preparative SEC Ultra-High Efficiency GFC/SEC Enjoy the same selectivity and ultra-high efficiency of Yarra 3 µm for your preparative gel filtration applications. Yarra SEC PREP features a µm particle size version of each Yarra 3 µm chemistry in a 1. mm ID column for preparative purification, desalting, and characterization of biomolecules. Yarra µm PREP is available at an affordable price while maintaining the high performance given with the analytical columns. uv 1, 1, 1, 8,,,, Increasing sample concentrations with fixed injection volume Injection Volume: 1 µl.1 mg/ml.1 mg. mg/ml. mg 1 mg/ml.1 mg mg/ml. mg 1 mg/ml 1. mg App ID min uv 18, 1, 1, 1, Increasing injection volumes with fixed sample concentration 1, 8,,,, Sample concentration: 1 mg/ml 1 µl.1 mg µl. mg 1 µl.1 mg µl. mg 1 ml 1 mg ml mg App ID min Conditions for both applications: Columns: Yarra μm SEC- PREP Dimensions: 3 x 1. mm Part No.: H-19-P Mobile Phase: 1 mm Sodium Phosphate ph.8 Flow Rate: ml/min Temperature: Ambient Detection: 8 nm (ambient) Sample: 1. Bovine Serum Albumin ( kda). Myoglobin (17 kda) 13

14 Select the Right Yarra Column for Your Application With four Yarra phases covering a large exclusion window, the Yarra product line offers the perfect solution for HPLC/UHPLC size exclusion chromatography of biomolecules. Molecular Weight (MW) Separation Ranges Native 1,-1,, 3,-7, 1,-3, X1 1,-, Molecular Weight (Daltons) 1,, Protein MW (Da) 1, Yarra 1.8 µm SEC-X1 Yarra 3 µm SEC- Yarra 3 µm SEC-3 Yarra 3 µm SEC- 1, Relative Elution Volume (ml/cv) Looking for a Yarra µm Analytical Column? We recommend using a 3 µm column to improve the resolution/sensitivity of your µm method. If method optimization is not an option, our BioSep µm GFC/SEC columns are a guaranteed alternative to your current µm column. Visit or contact your local representative for more details. 1

15 Ordering Information Ultra-High Efficiency GFC/SEC Yarra Column Specifications SEC-X1 SEC- SEC-3 SEC- SEC- PREP SEC-3 PREP SEC- PREP Resin Type Silica Silica Silica Silica Silica Silica Silica Particle Size (µm) Pore Size (Å) MW Range (Da) 1K-K 1K-3K K-7K 1K-1K 1K-3K K-7K 1K-1K ph Range Typical Backpressure (psi) 3, 1,3 1,3 1, Max Backpressure (psi) 7, 3, 3, 1,7 1, 1, 1, Typical Efficiency > 3, (1 x. mm) 8, (3 x 7.8 mm) 8, (3 x 7.8 mm) 38, (3 x 7.8 mm) 3, (3 x 1. mm) 3, (3 x 1. mm) 3, (3 x 1. mm) Max. Flow Rate (ml/min) NEW Yarra 1.8 µm SEC Column (mm) Analytical Phase 1 x. Yarra 1.8 µm SEC-X1 F-31-E Yarra 3 µm SEC Columns (mm) Narrow Bore Analytical Analytical SecurityGuard Cartridges (mm) Phases 3 x. 1 x x 7.8 x 3.* /1 pk Yarra 3 µm SEC- H-1-E F-1-K H-1-K AJ-87 Yarra 3 µm SEC-3 H-13-E F-13-K H-13-K AJ-88 Yarra 3 µm SEC- H-1-E H-1-K AJ-89 *SecurityGuard Analytical cartridges require holder, Part No.: KJ-8 for ID. 7.8 mm If Yarra analytical columns do not provide you with at least an equivalent separation as any other GFC column of similar porosity, type, and dimensions, return the column with comparative data within days for a FULL REFUND. Yarra µm PREP SEC Columns (mm) SecurityGuard Preparative Cartridges (mm) Phases 3 x 1. 1 x 1.** /ea Yarra µm SEC- PREP H-19-P AJ-888 Yarra µm SEC-3 PREP H--P AJ-889 Yarra µm SEC- PREP H-1-P AJ-89 **PREP SecurityGuard Cartridges require holder, Part No.: AJ-83 for ID 18 9 mm Terms and Conditions Subject to Phenomenex Standard Terms and Conditions, which may be viewed at Trademarks Yarra, BioSep, and SecurityGuard are trademarks of Phenomenex. TSKgel and Tosoh Bioscience are registered trademarks of Tosoh Corporation. ACQUITY, UPLC, and Waters are registered trademarks of Waters Corporation. Agilent is a registered trademark of Agilent Technologies, Inc. Shimadzu and Nexera are registered trademarks of Shimadzu Corporation. Disclaimer Phenomenex is in no way affiliated with Agilent Technologies, Tosoh Corporation, or Waters Corporation. Comparative separations may not be representative of all applications. SecurityGuard is patented by Phenomenex. U.S. Patent No.,1,3 CAUTION: this patent only applies to the analytical-sized guard cartridge holder, and does not apply to SemiPrep, PREP or ULTRA holders, or to any cartridges. 1 Phenomenex, Inc. All rights reserved. Phenomenex l WEB: 1

16 Ultra-High Efficiency GFC/SEC NEW Yarra 1.8 µm SEC-X1 Replace Waters BEH 1.7 µm SEC columns to: Save Money Minimize Adsorption Maximize Separation Range Australia t: +1 ()-98- f: +1 ()-98- Austria t: +3 () f: +3 () Belgium t: +3 () 3 1 (French) t: +3 () 11 8 (Dutch) f: +31 () beinfo@phenomenex.com Canada t: +1 (8) f: +1 (31) info@phenomenex.com China t: +8 () 8-8 f: +8 () chinainfo@phenomenex.com Denmark t: f: + 81 nordicinfo@phenomenex.com Finland t: +38 () f: + 81 nordicinfo@phenomenex.com France t: +33 () f: +33 () franceinfo@phenomenex.com Germany t: +9 () f: +9 () anfrage@phenomenex.com India t: +91 ()-31 f: +91 () indiainfo@phenomenex.com Ireland t: +33 ()1 7 f: eireinfo@phenomenex.com Luxembourg t: +31 ()3-187 f: +31 () nlinfo@phenomenex.com Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: +31 ()3-187 f: +31 () nlinfo@phenomenex.com New Zealand t: + () f: + ()9-789 nzinfo@phenomenex.com Norway t: f: + 81 nordicinfo@phenomenex.com Puerto Rico t: +1 (8) 1-HPLC f: +1 (31) info@phenomenex.com Spain t: f: espinfo@phenomenex.com Sweden t: + () f: + 81 nordicinfo@phenomenex.com United Kingdom t: + ()1-137 f: + ()1-179 ukinfo@phenomenex.com USA t: +1 (31) 1- f: +1 (31) info@phenomenex.com All other countries Corporate Office USA t: +1 (31) 1- f: +1 (31) info@phenomenex.com Italy t: f: italiainfo@phenomenex.com BR381_W Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com