BRANDplates Microplates

Transcription

1 BRANDplates BRAND has applied its decades of experience in premiumquality molding of lab plastic consumables from pipette tips to volumetric plasticware to cuvettes, to the challenges of microplate design. Plates are designed to minimize crosscontamination, ensure excellent recovery, and offer many options in color, well-count, surface-treatment and well-shape. Modern research methods require high-quality disposables. BRANDplates microplates from BRAND, can be used in all important fields of life science. For this comprehensive line, nine different surfaces have been developed under state of the art production conditions. The product line covers a multitude of standard applications (e.g., homogenous assays, screenings) as well as applications in the fields of immunology and cell culture technique. Non-treated surfaces puregrade puregrade S Immunology surfaces immunograde hydrograde lipograde Non-treated Cell culture surfaces cellgrade cellgrade plus cellgrade premium inertgrade Immunology Cell culture A comprehensive, premiumquality line of plates for cell culture and immunoassay BrandTech Scientific, Inc

2 BRANDplates Surfaces BRANDplates microplates are offered with 9 surface options. The surface modifications are generated via a physical-chemical process that creates stable surfaces for storage at room temperature. To enable easy identification of the surface type and well location, 96-well standard format plates have a color-coded embossing. All BRANDplates microplates are manufactured according to ANSI-/SLAS-Standards 1 to 4, 2004 and are free from endotoxins, DNase, DNA, and RNase. Sterilized plates are guaranteed to an SAL of Non-treated surfaces puregrade puregrade S Immunoassay surfaces immunograde hydrograde lipograde Cell culture surfaces cellgrade cellgrade plus cellgrade premium inertgrade non-treated, medium binding, non-sterile non-treated, medium binding, sterile ideal for immobilization of IgG, high binding, non-sterile hydrophilic, ideal for immobilization of hydrophilic molecules, non-sterile hydrophobic, ideal for immobilization of hydrophobic molecules, non-sterile standard tissue culture surface for the cultivation of adherent cell lines, sterile surface for the cultivation of fastidious adherent cells making serum-reduced cultivation possible, sterile surface treatment with similar properties to poly-d-lysine, serum-reduced or serum-free cultivation is possible, sterile surface that inhibits adhesion of cells, e.g., and reduces protein adsorption for cultivation of suspension and stem cells, sterile Color codes 96-well plates To enable an easy identification of the surface type and to facilitate the legibility of the alphanumeric coding BRANDplates microplates in the 96-well standard format have a color-coded embossing. All microplates have alphanumeric coding. Gray Coded Non-treated microplates for the most diversified applications Blue Coded for immunoassays such as ELISA, RIA, FIA, etc. Orange Coded for cell culture applications 84

3 BRANDplates Well-count, colors, and well-shape BRANDplates microplates are available in a variety of different colors, well-number, and well shapes. Different combinations of these characteristics create the broad product range of BRANDplates microplates. Well number BRANDplates microplates are offered in 96-, 384-, and well formats. BRANDplates microplates with 96-well standard format are manufactured with a chimney well design. Each individual well is separated from each other to reduce cross contamination risk. In addition, lids with condensation rings are available for substantial reduction in evaporative losses. In the 384- and 1536-well microplates, rounded wells allow optimal uniform meniscus formation. Colors and plate types The application to be performed in the plate generally determines the color of the plates used. A basic rule is: transparent for colorimetry; white for luminescence; and black for fluorescence. Plates are available as standard solid color plates or colored with transparent bottoms depending on the optical requirements of the application. 96-well white microplate 384-well black microplate 1536-well clear microplate Transparent bottom microplates Well shape 96-well BRANDplates microplates are offered with four distinct bottom shapes. 384-well and 1536-well microplates are only available with flat-bottom (F-bottom). Round (U-bottom): Round bottom plates have no edges and are suitable for stirring and washing of samples. This bottom shape is used for agglutination and other assays requiring these tasks. Flat (F-bottom): Flat bottom plates are designed for precise optical measurements. Useful for many applications, including cell culture of adherent cell types. Conical (V-bottom): Conical bottom plates are maximized for sample recovery after centrifugation, precipitation assays, and storage applications. Curved (C-bottom): The curved bottom is a flat bottom with curved edges. By combining the advantages of round and flat bottom plates, better sample mixing is obtained with precise optical measurements for immunological assays. Round bottom (U) Flat bottom (F) Conical bottom (V) Curved bottom (C) 85

4 BRANDplates Samples and plate selection Given the broad range of plates available, we would like to provide maximum assistance in the selection of the optimum plate for your application. Use BRAND s online plate selection guide at to select the right plate for your needs. Samples are available without cost or obligation. Barcodes BRAND can provide high-quality and customized barcode labeling for your plate needs. Visit for more information Accessories BRAND offers many accessories for use with microplate products such as lids, sealing films, and more. Lids with and without condensation rings are optimally matched to the corresponding BRANDplates microplate to protect samples. For enhanced protection against contamination and evaporation, self-adhesive sealing films are available in a variety of materials and can be easily applied with a rubber roller. Condensation Rings Height Sterile Pack of Cat. No. Lids for 96-well standard plates Yes 8mm No Pack of 100 (5 lids per bag) $ No 8mm No Pack of 100 (5 lids per bag) For BRANDplates microplates Cat. No. ranges: , , , , , , , , Lids for 96-well plates with transparent bottom and all 384-well plates Pack of 50 lids No 4.5mm No (10 lids per bag) For BRANDplates microplates Cat. No. ranges: , , , , , , , , Lids for all 1536-well plates No 5.5mm No Pack of 50 lids (10 lids per bag) For BRANDplates microplates Cat. No. ranges: , , Application Description Material Pack of Cat. No. Sealing films, Self-adhesive ELISA, PCR single film polypropylene 100 sheets $ ELISA, Real-Time PCR single film, highly transparent polyester 100 sheets Inquire NEW! Cold storage roll with 100 sheets aluminum 1 roll Cold storage single film aluminum 100 sheets Cell and tissue culture single film, gas-permeable rayon 100 sheets Cell and tissue culture single film, gas-permeable, sterile rayon 50 sheets Storage single film, DMSO-resistant polypropylene 100 sheets Automation single film PE/PP 50 sheets Storage, fluorescence measurements single film vinyl 50 sheets Luminescence measurements single film vinyl 50 sheets Roller for sealing films

5 BRANDplates BRANDplates microplates at a glance 96-well Type Non-treated Immunological surfaces Cell culture surfaces Standard transparent U / 330µL transparent V / 360µL transparent F / 350µL transparent C / 350µL white U / 330µL white F / 350µL white C / 350µL black U / 330µL black F / 350µL black C / 350µL With transparent bottom white F / 330µL black F / 330µL Strip plates transparent, F / 360µL without grid transparent, F / 350µL with grid 384-well HTS Type Non-treated Immunological surfaces Cell culture surfaces Standard transparent F / 100µL white F / 100µL black F / 100µL With transparent bottom white F / 120µl black F / 120µl well UHTS Type Non-treated Immunological surfaces Cell culture surfaces Standard transparent F / 10µL white F / 10µL black F / 10µL Interactive Selection Guide with more information about BRANDplates microplates available at For plate selection guide go to 87

6 BRANDplates for Immunoassays Suitable for ELISA, RIA, FIA and Other Immune Testing immunograde TM Optimized for the immobilization of IgG hydrograde TM For the immobilization of hydrophilic molecules Optimized for the immobilization of IgG, offering highest binding capacity for molecules with mixed hydrophilic and hydrophobic regions. The surface of choice for the majority of standard ELISAs. Suitable for solid phase immunoassays. high-binding. PS, non-sterile. Strip plates available (see page 89). Strongly hydrophilic, with high affinity to hydrophilic molecules, such as glycoproteins and peptides, antibodies with predominantly hydrophilic regions, and nucleic acids. An alternative to the immunograde surface when performing solid phase assays. Alternative for homogeneous assays with hydrophobic molecules that remain in solution. PS, non-sterile. For solid phase with hydrophilic molecules and liquid phase with hydrophobic molecules, respectively. immunograde TM Color Bottom Well volume Cat. No. 96-well standard microplates transparent U-bottom 330µL $ transparent V-bottom 360µL transparent F-bottom 350µL transparent C-bottom 350µL white U-bottom 330µL white C-bottom 350µL black U-bottom 330µL black C-bottom 350µL well transparent bottom microplates black F-bottom 330µL well standard microplates transparent F-bottom 100µL white F-bottom 100µL black F-bottom 100µL hydrograde TM Color Bottom Well volume Cat. No. 96-well standard microplates transparent U-bottom 330µL $ transparent F-bottom 350µL transparent C-bottom 350µL white U-bottom 330µL white C-bottom 350µL black U-bottom 330µL black C-bottom 350µL well standard microplates transparent F-bottom 100µL white F-bottom 100µL black F-bottom 100µL

7 BRANDplates for Immunoassays lipograde TM For the immobilization of hydrophobic molecules Strongly hydrophobic (lipophilic), for immobilization of biomolecules with predominantly hydrophobic regions. An alternative to the immunograde surface for the immobilization of molecules such as lipoproteins or peptides. Specially suited for liquid phase assays when the reaction component should stay in solution. The majority of hydrophilic biomolecules are not immobilized on this surface. PS, non-sterile. For solid phase with hydrophobic molecules and liquid phase with hydrophilic molecules, respectively. 96-well standard-volume microplates lipograde TM Color Bottom Well volume Cat. No. 96-well standard microplates transparent U-bottom 330µL $ transparent V-bottom 360µL transparent F-bottom 350µL transparent C-bottom 350µL white U-bottom 330µL white C-bottom 350µL black U-bottom 330µL black C-bottom 350µL well transparent bottom microplates white F-bottom 330µL black F-bottom 330µL well standard microplates transparent F-bottom 100µL white F-bottom 100µL black F-bottom 100µL well transparent bottom microplates white F-bottom 120µL black F-bottom 120µL well transparent bottom microplates 384-well standard-volume microplates 91