Product Datasheet. Perilipin Antibody NB Unit Size: 0.1 ml

Transcription

1 Product Datasheet Perilipin Antibody NB Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 6 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 2/20/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Perilipin Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer Target Molecular Weight 0.1 ml 1.1 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Polyclonal 0.05% Sodium Azide IgG Immunogen affinity purified Tris-Glycine and 0.15M NaCl 60 kda Page 1 of 6 v.20.1 Updated 2/20/2019 Product Description Host Rabbit Gene ID 5346 Gene Symbol Species Immunogen Product Application Details Applications PLIN1 Human, Mouse, Rat, Porcine A synthetic peptide made to a region between residues (C-terminus) of the human Perilipin protein. [Swiss-Prot# O60240] Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin Recommended Dilutions Western Blot 2 ug/ml, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:200 Application Notes This Perilipin antibody is useful for Western blot anlaysis where a band at ~60 kda is seen. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

3 Images Western Blot: Perilipin Antibody [NB ] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS- PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/ml anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence. Page 2 of 6 v.20.1 Updated 2/20/2019 Immunohistochemistry-Paraffin: Perilipin Antibody [NB ] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells. Immunohistochemistry-Paraffin: Perilipin Antibody [NB ] - IHC analysis of formalin fixed paraffin embedded section of fat tissue from mouse with Perilipin antibody at 1:200 dilution. The antibody generated an expected staining in the adipocytes towards periphery of the cells. Immunohistochemistry-Paraffin: Perilipin Antibody [NB ] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.

4 Publications Listenberger L, Townsend E, Rickertsen C et al. Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis. Cells. Nov :00AM [PMID: ] (WB, Mouse) Finlin BS, Memetimin H, Confides AL et al. Human adipose beiging in response to cold and mirabegron. JCI Insight 2018 Aug 9 [PMID: ] (IHC, Human) Details: This citation used the Alexa Fluor 488 version of this antibody. Andrei SR. Characterization of TRP Ion Channels in Cardiac Muscle. Thesis (WB, Mouse) Page 3 of 6 v.20.1 Updated 2/20/2019 Maisano M, Cappello T, Oliva S et al. PCB and OCP accumulation and evidence of hepatic alteration in the Atlantic bluefin tuna, T. thynnus, from the Mediterranean Sea. Mar Environ Res 2016 Oct [PMID: ] Manzano-Roman, R et al. Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism. J Med Microbiol 57(Pt 2): Feb. [PMID: ] (ICC/IF, Human) Kadereit, B et al. Evolutionarily conserved gene family important for fat storage. Proc Natl Acad Sci U S A 105(1): Jan 8. [PMID: ] (WB, Mouse)

5 Procedures Western Blot protocol for Perilipin Antibody (NB ) Western Blot Protocol Page 4 of 6 v.20.1 Updated 2/20/ Perform SDS-PAGE with a 12% gel on samples to be analyzed, loading 30 ug of total protein per lane. 2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus. 3. Rinse membrane with dh2o and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil. 4. Rinse the blot in TBS for approximately 5 minutes. 5. Block the membrane using 5% non-fat dry milk + 1% BSA in TBS, overnight at 4 degrees Celcius. 6. Rinse the membrane in dh2o and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each. 7. Dilute the rabbit anti-perilipin primary antibody (NB ) in blocking buffer and incubate 2 hours at room temperature. 8. Rinse the membrane in dh2o and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each. 9. Apply the diluted rabbit-igg HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature. 10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background). 11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce's ECL). Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of %, provided it does not interfere with antibody-antigen binding.

6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NB NB PEP HAF008 NB7160 NBP Human Liver Whole Tissue Lysate (Adult Whole Normal) Perilipin Blocking Peptide Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody [HRP] Rabbit IgG Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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