CCM Approval: IACUC Approval:

Transcription

1 Title: Investigator Transportation of Live Mice or Rats Outside Holding CCM Approval: IACUC Approval: 1. Purpose: The purpose of this SOP is to describe local transportation of live laboratory mice and rats from CCM facilities to IACUC approved satellites and laboratory use areas Transportation of animals by CCM staff between CCM-managed animal facilities is covered in BWH-SOP-HUS-227, Rodent Transfer Process Animal transport from CCM to an institution outside the CCM program or BWH laboratory spaces requires IACUC approval and may require an endorsed health certificate, e.g., for international or other shipments BL2-N 72 hour and transfer of BL2-N animals to centralized, shared procedure areas (i.e. SAIL, Imaging crescent, BL-1/BL-2 dual use procedure rooms) follow BWH-SOP-RES-507 Removal of BL2N rodents from holding space 2. Scope: Animal care staff Investigative staff 3. Definitions: BSL Biosafety Level BL1-N CCM rodent holding facility Animal biosafety level 1 (standard rodent housing) BL2-N CCM rodent holidng facility Animal Biosafety Level 2 (HIM B53, B54, B55, B56; BTM C, and Thorn 1616, SAIL) Primary container container in contact with the animal. Secondary container rigid, escape proof container with high efficiency particulate filter. Centralized holding facility BWH vivarium managed by the Center for Comparative Medicien Core (HIM basement, BTM 11 th floor, MCP room 65/67 Thorn) Rodent Barrier facility Centralized rodent holding facility that has highest level of bioexclusion practices (HIM, BTM, MCP 65) Rodent non-barrier facility Centralized rodent facility with lower bioexclusion practices (Thorn, HIM in/out B46B, BTM in/out 11-26, SAIL) BWH-SOP-RES-500 V02 Page 1 of 5 Effective Date: 07/11/2017

2 4. Equipment/Supplies: Primary transport container CCM provided cardboard containers or CCM provided static microisolator transport Disposable (Innovive) microisolator caging (for investigator BL2N transport) Moistened food or gel Rubber band or tape Secondary transport container CCM provided paper or plastic bag or lab provided covered transport card or sanitizable bin 5. Procedures: 5.1. Centralized Transport between CCM core vivaria: Complete animal bioware order request for rodent transfer using the following URL px Additional instructions for using bioware can be accessed using the CCM core website animal.bwh.harvard.edu Follow and respond to ed instructions to complete rodent transfer request The following types of rodent transfer/transport must be conducted only by CCM staff after sumitting Bioware transfer request Movement of animals between CCM centralized BL1-N holding facilities Movement of animals from CCM centralized BL1-N to BL2-N holding facilities Movement of animals from CCM centralized BL2-N to BL-2N holding facility 5.2. Investigators my conduct the following types of rodent transfer independently following the procedures outlined in section 5.3: Transfer of rodents from BL1-N central (barrier or non-barrier) rodent holding facility to IACUC approved lab use area for terminal use Transfer of rodents from BL1-N central (barrier or non-barrier) rodent holding facility to IACUC approved lab use area or core for survival procedure and return to CCM centralized non-barrier rodent holding area (i.e. in/out room) Transfer of BL2-N rodents from centralized BL-2N holding facility to IACUC approvced BL-2 lab use area for terminal procedures (transport of BL2 animals must be described in IACUC protocol under Transport within Institution Form ). 1BWH-SOP-RES-500 V02 Page 2 of 5

3 5.3. Transport Procedures when conducted by investigators: Minimize transit time Animals must not be visible to public Avoid public areas as much as possible, including congested passages When possible, avoid human patient areas such as the Pike Avoid transporting during weather and temperature extremes refer to Chart 1. Chart 1: Allowable temperature parameters for outdoor transfers Below 15 F 15 to 32 F 32 to 80 F 80 to 95 F Above 95 F Not permitted* Permitted provided that animals are exposed to the outdoor for no more than 5 minutes* Permitted Permitted provided that animals are exposed to the outdoor for no more than 5 minutes* Not permitted* *Transfers are permitted regardless of outdoor temperature if moved in an IACUC inspected climate-controlled vehicle Avoid transport of the animal with any other animal, substance, or device that may potentially cause injury Minimize vibrations that may stress animals especially when utilizing transport cart Rubberized padding placed under cages significantly reduces cage level vibrations Provide food and a source of water if rodents will be held within the transport container for longer than 4 hours Provide adequate ventilation Only move animals to use areas that are listed on an approved IACUC protocol 5.4. Transport of BL1 animals, nucleic acids, proteins, and non- toxic substances: Use the disposable, cardboard, animal transport containers provided by CCM Secure lid with tape Place cardboard container inside secondary container such as a plastic bin, microisolator cage, or CCM provided paper/plastic bag 5.5. Transport of animals through Human Patient Areas (e.g., Clinical MRI, the Pike) Secondary animal transport containers must include high-efficiency filter media (microisolator) Transport Procedures for animals administered toxic agents 1BWH-SOP-RES-500 V02 Page 3 of 5

4 Use the disposable animal transport containers (opaque cardboard container) provided by CCM Disposable animal transport containers must be placed in a rigid outer carrier with escape proof lid for secondary containment There must be a means to prevent unintended opening, such as a latch or locking mechanism, tape, rubber band or a box-within-a-box Transport carriers must be easily decontaminated or disposable. The disposable components must be disposed of in biohazard waste bins or box 5.7. Transport Procedures for animals infected with BL2 agents The Biosafety Officer (BSO) must be notified and approve the transportation of animals that have been administered BL2 pathogens. Approval is based on a risk assessment and may include additional recommendations beyond what is covered in this SOP and the general transport procedures described here Notify JHEALEY1@partners.org and CCM before these animals are being prepared for transport Use disposable, primary animal cages and secure lids with rubber bands Example of acceptable source of disposable caging The outser surfaces of disposable cages must be disinfected before transport by applying appropriate disinfectant before leaving the animal facility. No visible contamination (blood, tissue, feces, etc) can be present on outer survaces Identify the specific infectious agent with a label and biohazard warning label on the disposable cage Disposable cages must be placed in an opaque container (such as a brown paper bag) so that the animals are not visible A sturdy cart is recommended for movement of cages to remove the risk of dropping The disposable, primary animal cages must be disposed of in BL2 biohazard waste bins or box References: BWH-SOP-HUS Rodent Transfer Process Committee on Guidelines for the Humane Transportation of Laboratory Animals, N.R.C. Guidelines for the Humane Transportation of Research Animals. The National Academies Press, Washington, D.C (Pre-publication copy) 1BWH-SOP-RES-500 V02 Page 4 of 5

5 University of Michigan Medical School Animal Transportation SOP. National Research Council Guide for the Care and Use of Laboratory Animals. 8 th Edition. Washington, D.C.: National Academy Press. National Institutes of Health Animal Transportation Guidelines. Report of the Transport Working Group established by the Laboratory Animal Science Association (LASA). Guidance on the transport of laboratory animals, Laboratory Animals, 39: 1-39, Document History Version Revisions Date 00 New 08/01/ Minor revisions including updates to ABSL-2 language 01/08/ Significant Changes and SOP restructuring 10/24/2017 1BWH-SOP-RES-500 V02 Page 5 of 5